BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0132.p (684 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313469|ref|YP_003777561.1| fimbrial type-4 assembly signal... 883 0.0 gi|300310140|ref|YP_003774232.1| type II/III HrcC transmembrane ... 88 2e-18 >gi|300313469|ref|YP_003777561.1| fimbrial type-4 assembly signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 674 Score = 883 bits (2282), Expect = 0.0, Method: Compositional matrix adjust. Identities = 469/681 (68%), Positives = 548/681 (80%), Gaps = 11/681 (1%) Query: 4 ILFLLALLASHAALAENRLLAIHLHPRADGTTLELQLQAPVDPALVQNFLLIEPPQLVLD 63 +L + L+ S ALA RL A+ + R + TTL L+L + + + +F L++PPQLVLD Sbjct: 5 LLLMGVLILSAPALAGPRLHALDIEARDEATTLTLRLDQTLPLSQIHHFQLLDPPQLVLD 64 Query: 64 LPRSTAAATLARSLSGSGLVKRIQLAEDDDRLRLQVLLKHAAIYQLRAEEDRLLVDLTLQ 123 LP +T AA +AR + SGL+KR+ LA D +RLRL LK YQL+ +++++ V L+ Sbjct: 65 LPDTTLAAGMARQFAASGLLKRVTLASDGERLRLLATLKRPLHYQLQVQDEQVQVALSPL 124 Query: 124 PPSDPVALQAIDLRRGPQGEAHLAVDMGSHAHTVQVRQLGGRLVVDIAGATVPLALRRLV 183 PP P AL I +RG GE L +D+GS A VQVRQ G RL+VDI G T+P R V Sbjct: 125 PPQAPTALGGIGFQRGSAGEGRLDIDLGSTAPVVQVRQQGQRLIVDIGGTTLPTGSARQV 184 Query: 184 ETGQMGTPVLQYRAYGHQDGAXPVQGRRLVLEMQGQWRYQAYQSKRRLMIDVVPVKSALT 243 E G TPV QY+AY +++G RL + QG WRYQA Q ++L IDV+PV + Sbjct: 185 EVGDFATPVTQYQAYPYENGV------RLAIHAQGLWRYQAVQRGQQLRIDVLPVSADPH 238 Query: 244 GIAAAGLQKGKTYGGPRLSLNFQNIEVRTALQVLAEHSGVNIVASDTVTGQLSLRLKDLP 303 IA K Y G RLSLNFQNIEVRTALQVLA+ +G+NI+ASD VTGQLSLRLKD+P Sbjct: 239 RIA-----PDKDYQGARLSLNFQNIEVRTALQVLADFTGLNIIASDAVTGQLSLRLKDVP 293 Query: 304 WEQVLDLILQARGLEMRRHGEVLWIAPRDEMLARERLELEQKAMIADLEPLHAESFQLNY 363 WEQ L++ILQARGL ++R G VLWIAPR+EM ARER ELEQKA++ADLEPLHAE+FQLNY Sbjct: 294 WEQALEMILQARGLALQRSGGVLWIAPREEMAARERQELEQKALLADLEPLHAETFQLNY 353 Query: 364 QRADTLRKALGIGEDGSTQANRRNALLSRRGSAMIDSHTNQLLVTDTLAVLANVRKLIER 423 QRAD LR+ALG+GEDG+ QANRR +LLSRRGSAMIDSHTNQLLVTDTLA LANVRKLIER Sbjct: 354 QRADNLRRALGLGEDGTPQANRRASLLSRRGSAMIDSHTNQLLVTDTLAALANVRKLIER 413 Query: 424 IDVASRQVLIEARIVEADDSFSRNLGIKLGLAATTRGAAVGNGYAAIAEQSGQTPATPDL 483 ID+A+RQV+IEARIVEADDSF+RNLG+KLGLAA T+GAA+GN YA++ E SGQTP Sbjct: 414 IDIAARQVMIEARIVEADDSFARNLGVKLGLAARTQGAALGNSYASVGELSGQTPPAAGT 473 Query: 484 TLREPALNLRADGLSGAAPGSFAFTLFNAAARRFLNVELSALEADGRGKIVSSPRLITAD 543 LREPAL L A L+G+APGSFAFTLFNAAA+RFLNVELSALEA+GRGKIVSSPRL+TAD Sbjct: 474 YLREPALGLGAGALAGSAPGSFAFTLFNAAAQRFLNVELSALEAEGRGKIVSSPRLVTAD 533 Query: 544 QQAALIEQGEEIPYQQSAGNGSTATAFKKANLKLEVTPQITPDGNVILDVDVNKDSRGSV 603 QQAALIEQGEEIPYQQS G+G+TATAFKKANLKLEVTPQITPDGNVILDVDVNKDSRGS Sbjct: 534 QQAALIEQGEEIPYQQSIGSGATATAFKKANLKLEVTPQITPDGNVILDVDVNKDSRGSA 593 Query: 604 TPGGLAINTKHIKTKVQVEDGGTVVIGGIYTQTDNDHETRVPLLGDLPVLGALFRSQSKV 663 TPGGLAINTKHIKTKVQVEDGGTVVIGGIYTQTDN+HETRVPLLG++P+LGALFRS++KV Sbjct: 594 TPGGLAINTKHIKTKVQVEDGGTVVIGGIYTQTDNEHETRVPLLGEIPLLGALFRSRNKV 653 Query: 664 RDKTELLIFLTPRIVAGPRPY 684 RDKTELLIFLTPRIVA PRPY Sbjct: 654 RDKTELLIFLTPRIVAPPRPY 674 >gi|300310140|ref|YP_003774232.1| type II/III HrcC transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 693 Score = 88.2 bits (217), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 87/298 (29%), Positives = 137/298 (45%), Gaps = 46/298 (15%) Query: 399 DSHTNQLLVTDTLAVLANVRKLIERIDVASRQVLIEARIVEADDSFSRNLGIKLGLAATT 458 DS N +++ D +A LI ++DV +QV IEA IV+ D S +G++ G Sbjct: 307 DSSLNAIMIYDDIAKRDMYAALINQLDVKPQQVEIEALIVDIDRSRLAEMGVEWG----A 362 Query: 459 RGAAVGNGYAAIAEQSGQTPATPDLTLREPALNLRADGLSGAAPGSFAFTLFNAAARRFL 518 R V QS T DL L PG+ TL + A RF Sbjct: 363 RAGVVNTTVNGTTTQSSGT----DLPL----------------PGA---TLLISDAARFY 399 Query: 519 NVELSALEADGRGKIVSSPRLITADQQAALIEQGEEIPYQQSAGNGSTATAFKKANLKLE 578 L A+E++G +++++P ++T D AA+++ + Y G + A L Sbjct: 400 -ARLKAMESNGEARVLATPTVLTLDNVAAVLDLSQSA-YVSLIGERVADMSDITAGTMLR 457 Query: 579 VTPQITPDGN---VILDVDVNKDS-------------RGS-VTPGGLAINTKHIKTKVQV 621 V P+I DG V L+VD+ S RG+ + ++ I T+ + Sbjct: 458 VIPRIIDDGGQTRVRLEVDIEDGSLNNGNNDSGSNSSRGTGASTIAASVTRSTINTQAII 517 Query: 622 EDGGTVVIGGIYTQTDNDHETRVPLLGDLPVLGALFRSQSKVRDKTELLIFLTPRIVA 679 + T++IGG ++ ++ +VPLLGDLPV+G LFRS+S+ E L +TPRI + Sbjct: 518 DAQQTLMIGGYRAESLIRNKQKVPLLGDLPVVGGLFRSESRSNSTRERLFLITPRITS 575 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,074,965 Number of extensions: 89683 Number of successful extensions: 303 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 300 Number of HSP's successfully gapped: 2 Length of query: 684 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 592 Effective length of database: 1,184,250 Effective search space: 701076000 Effective search space used: 701076000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)