BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0109.p (298 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313519|ref|YP_003777611.1| 6-phosphogluconate dehydrogenas... 580 e-167 gi|300312370|ref|YP_003776462.1| 3-hydroxyisobutyrate dehydrogen... 161 5e-41 gi|300310292|ref|YP_003774384.1| 3-hydroxyisobutyrate dehydrogen... 112 5e-26 gi|300311332|ref|YP_003775424.1| dehydrogenase [Herbaspirillum s... 69 5e-13 >gi|300313519|ref|YP_003777611.1| 6-phosphogluconate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 298 Score = 580 bits (1496), Expect = e-167, Method: Compositional matrix adjust. Identities = 289/298 (96%), Positives = 294/298 (98%) Query: 1 MSKNVGVIGLGAMGYGVASSLLRAGFNVHACDVRKEVLDKFAAAGGVACANPAELAAKVD 60 MS+NVGVIGLGAMGYGVASSLLRAGFNVHACDVRKEVLDKFAAAGGVACANPAELA KVD Sbjct: 1 MSRNVGVIGLGAMGYGVASSLLRAGFNVHACDVRKEVLDKFAAAGGVACANPAELAGKVD 60 Query: 61 VVITLVVNAAQTEMVLFGENGAVSAMKPGSVVISSATVAPDFAVELGKRLAEKDLLLLDA 120 VVITLVVNAAQTE VLFG+NGAVSAMKPGSVVISSATVAPDFA+ELGKRL EK LLLLDA Sbjct: 61 VVITLVVNAAQTETVLFGDNGAVSAMKPGSVVISSATVAPDFAIELGKRLVEKGLLLLDA 120 Query: 121 PVSGGAARAASGEMTMMTSGPAEVYAKIEDVLAGMAGKVYRLGDTHGIGSKVKIINQLLA 180 PVSGGAARAASGEMTMMTSGPAEVYAKIEDVLAGMAGKVYRLGDTHGIGSKVKIINQLLA Sbjct: 121 PVSGGAARAASGEMTMMTSGPAEVYAKIEDVLAGMAGKVYRLGDTHGIGSKVKIINQLLA 180 Query: 181 GVHIAASAEAMALGLREGVNPDALYDVITHSAGNSWMFENRVPHILNGDYTPLSAVDIFV 240 GVHIAASAEAMALGLREGVNPDALY+VITHSAGNSWMFENRVPHIL+GDYTPLSAVDIFV Sbjct: 181 GVHIAASAEAMALGLREGVNPDALYEVITHSAGNSWMFENRVPHILSGDYTPLSAVDIFV 240 Query: 241 KDLGLVLDTARRSKFPLPLSAAAHQMFMMASTAGHGGEDDSAVIKIFPGIDLPPAKAK 298 KDLGLVLDTARRSKFPLPLSAAAHQMFMMASTAGHGGEDDSAVIKIFPGIDLPPAKAK Sbjct: 241 KDLGLVLDTARRSKFPLPLSAAAHQMFMMASTAGHGGEDDSAVIKIFPGIDLPPAKAK 298 >gi|300312370|ref|YP_003776462.1| 3-hydroxyisobutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 297 Score = 161 bits (408), Expect = 5e-41, Method: Compositional matrix adjust. Identities = 97/294 (32%), Positives = 161/294 (54%), Gaps = 8/294 (2%) Query: 1 MSKNVGVIGLGAMGYGVASSLLRAGFNVHACDVRKEVLDKFAAAGGVACANPAELAAKVD 60 MS N+ IGLG MG +A +L++AGF+V D+ K +DK GG A+ AK D Sbjct: 1 MSANIVFIGLGNMGLPMAQNLVKAGFSVSGHDLVKASVDKLVEVGGKTEADSMAAVAKAD 60 Query: 61 VVITLVVNAAQTEMVLFGENGAVSAMKPGSVVISSATVAPDFAVELGKRLAEKDLLLLDA 120 VVIT++ + E + GE G +++ KPG+++I +T+AP+ A ++ + +LDA Sbjct: 61 VVITMLPASRHVESLYLGEAGVLASAKPGTLLIDCSTIAPEAARKVAAAAKARGFEMLDA 120 Query: 121 PVSGGAARAASGEMTMMTSGPAEVYAKIEDVLAGMAGKVYRLGDTHGIGSKVKIINQLLA 180 PVSGG A +G +T M G E + + L M +Y G++ G G VK+ N +L Sbjct: 121 PVSGGTNGATAGTLTFMVGGSKEAFEAAQPYLQKMGKAIYHAGES-GSGQTVKVCNNMLL 179 Query: 181 GVHIAASAEAMALGLREGVNPDALYDVITHSAGNSWMFE--NRVPHIL-----NGDYTPL 233 G+ + ++EA+ LG+ G++P L ++++ S+G +W E N P ++ + Y Sbjct: 180 GILMIGTSEAIRLGMANGMDPKVLSEIMSKSSGRNWTLEVYNPCPGVMETAPASKGYAGG 239 Query: 234 SAVDIFVKDLGLVLDTARRSKFPLPLSAAAHQMFMMASTAGHGGEDDSAVIKIF 287 VD+ +KDLGL ++ A + +PL A A ++ + S G G D S++ + Sbjct: 240 FGVDLMLKDLGLAVENALATNCAIPLGATARNLYDIHSMNGAGKLDFSSIFNML 293 >gi|300310292|ref|YP_003774384.1| 3-hydroxyisobutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 288 Score = 112 bits (279), Expect = 5e-26, Method: Compositional matrix adjust. Identities = 84/266 (31%), Positives = 132/266 (49%), Gaps = 2/266 (0%) Query: 3 KNVGVIGLGAMGYGVASSLLRAGFNVHACDVRKEVLDKFAAAGGVACANPAELAAKVDVV 62 K +GVIGLG MG G+A SL R G+ V D ++ A G + A LA V++V Sbjct: 2 KKIGVIGLGNMGRGMAFSLQRGGYEVLGYDAQESTRTALAQQGLHCVDSIAALAPAVEMV 61 Query: 63 ITLVVNAAQTEMVLFGENGAVSAMKPGSVVISSATVAPDFAVELGKRLAEKDLLLLDAPV 122 + + + E V+ GE G + PG V++ + T P+ L RL + L+DAPV Sbjct: 62 VLSLPTSDIVEQVVEGEGGLLQHGAPGLVIVDTTTADPNSTRRLAARLQAAGMELVDAPV 121 Query: 123 SGGAARAASGEMTMMTSGPAEVYAKIEDVLAGMAGKVYRLGDTHGIGSKVKIINQLLAGV 182 SGG AA+ M M+ G AE A+IE VLA M+ K +G G G K++N L+ G Sbjct: 122 SGGPKGAATATMGMVIGGSAETVARIEPVLAVMSAKRVHVGPV-GAGHVAKLLNNLVTGT 180 Query: 183 HIAASAEAMALGLREGVNPDALYDVITHSAGNSWMFENRVP-HILNGDYTPLSAVDIFVK 241 H+ + EA + G++ ++ + + NS + E P I N + + + K Sbjct: 181 HLLIAGEAARIAAAAGLDARQIFQGLAAGSANSKVIEVFYPTWIFNEAFDSGFTMKLMRK 240 Query: 242 DLGLVLDTARRSKFPLPLSAAAHQMF 267 DL L ++ ++ PL+ A +++ Sbjct: 241 DLRLAMNLIEETRSTTPLAREAGRLW 266 >gi|300311332|ref|YP_003775424.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 292 Score = 68.9 bits (167), Expect = 5e-13, Method: Compositional matrix adjust. Identities = 73/283 (25%), Positives = 146/283 (51%), Gaps = 2/283 (0%) Query: 4 NVGVIGLGAMGYGVASSLLRAGFNVHACDVRKEVLDKFAAAGGVACANPAELAAKVDVVI 63 + +GLG MG+ +A++L+RAG+ V A + +++ AA G A A PAE AA DV+I Sbjct: 2 KIAFLGLGTMGFPMAANLVRAGYAVRAWNRSPAPVERLAALGAQAAATPAEAAADADVLI 61 Query: 64 TLVVNAAQTEMVLFGENGAVSAMKPGSVVISSATVAPDFAVELGKRLAEKDLLLLDAPVS 123 +++ + TE L A++A++PG++ ++ AT++ A+ L + + + APV Sbjct: 62 SMLADDDATETSLLDAG-ALAALRPGAIHVNMATISVALALRLAGLHQARGVAYVAAPVL 120 Query: 124 GGAARAASGEMTMMTSGPAEVYAKIEDVLAGMAGKVYRLGDTHGIGSKVKIINQLLAGVH 183 G A +G++ ++ +G + A ++ +L + K +RLG + K+ + Sbjct: 121 GRVNVAEAGQLNILAAGEEQALAAVQPLLDVLGQKTWRLGRQPEQANAAKLAMNFMIASA 180 Query: 184 IAASAEAMALGLREGVNPDALYDVITHSAGNSWMFENRVPHILNGDYTPLS-AVDIFVKD 242 I A +EA+AL G++ ++ T +A + +++ I + + P + + +KD Sbjct: 181 IGAMSEAVALVQGYGLDKAGFIELATSTAFAAPVYKGYGQAIADDRFEPAGFKLALGLKD 240 Query: 243 LGLVLDTARRSKFPLPLSAAAHQMFMMASTAGHGGEDDSAVIK 285 + L L+ ++ PL L++A + + G G D +A+ + Sbjct: 241 VRLALEAGEQAHVPLSLASALRDLHIDGLAHGEGHLDWAALSR 283 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 915,882 Number of extensions: 36430 Number of successful extensions: 200 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 195 Number of HSP's successfully gapped: 4 Length of query: 298 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 213 Effective length of database: 1,217,395 Effective search space: 259305135 Effective search space used: 259305135 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)