BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0094.p (260 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313535|ref|YP_003777627.1| amino acid ABC transporter/sign... 496 e-142 gi|300311590|ref|YP_003775682.1| amino acid ABC transporter/sign... 303 1e-83 gi|300310886|ref|YP_003774978.1| amino acid ABC transporter peri... 273 1e-74 gi|300309582|ref|YP_003773674.1| amino acid ABC transporter/sign... 182 2e-47 gi|300313169|ref|YP_003777261.1| amino acid transport periplasmi... 175 3e-45 >gi|300313535|ref|YP_003777627.1| amino acid ABC transporter/signal transduction system periplasmic protein/domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 260 Score = 496 bits (1277), Expect = e-142, Method: Compositional matrix adjust. Identities = 248/260 (95%), Positives = 255/260 (98%) Query: 1 MKLTKLLLGLMAGAMMFSASAARADALDDIQKHGTLRVAVPADFPPFGSVDSDLKPLGFD 60 MKLTKLLLGLMAGAMMFSAS ARADALDDIQKHGTLRVAVPADFPPFGSVD+DLKP G D Sbjct: 1 MKLTKLLLGLMAGAMMFSASVARADALDDIQKHGTLRVAVPADFPPFGSVDADLKPQGLD 60 Query: 61 IDVANLIAKKMGVKVELIPVSSANRIPYLTTKKVDLVISSMGKNAEREKVIDFSAAYAPF 120 IDVA LIAKK+GVKVELIPVSSANRIPYLTTKKVDLVISSMGKNAEREKVIDFSAAYAPF Sbjct: 61 IDVATLIAKKLGVKVELIPVSSANRIPYLTTKKVDLVISSMGKNAEREKVIDFSAAYAPF 120 Query: 121 FNGVFGPADLPVKSAADLAGKTIAVTRGSVEDLELTKIVPASATVKRYEDNNSTISSFLS 180 FNGVFGPADL VKSAADLAGKTIAVTRGSVEDLELTKIVPASATVKRYEDNNSTISSFLS Sbjct: 121 FNGVFGPADLSVKSAADLAGKTIAVTRGSVEDLELTKIVPASATVKRYEDNNSTISSFLS 180 Query: 181 GQVQLVATGNVVAAAIIAKNPPKKPETKFLIKDSPCFIGLNKGEKKLQDKVDAILADAKK 240 GQVQLVATGNVVAAAIIA+NPPKKPETKFLIKDSPCFIGLNKGEKKLQDKVDAILA++KK Sbjct: 181 GQVQLVATGNVVAAAIIARNPPKKPETKFLIKDSPCFIGLNKGEKKLQDKVDAILAESKK 240 Query: 241 DGSLNAISQKWLGLPLPAGL 260 DGSL+AISQKWLG+PLPAGL Sbjct: 241 DGSLSAISQKWLGMPLPAGL 260 >gi|300311590|ref|YP_003775682.1| amino acid ABC transporter/signal transduction system periplasmic protein/domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 267 Score = 303 bits (776), Expect = 1e-83, Method: Compositional matrix adjust. Identities = 150/260 (57%), Positives = 188/260 (72%) Query: 1 MKLTKLLLGLMAGAMMFSASAARADALDDIQKHGTLRVAVPADFPPFGSVDSDLKPLGFD 60 M K +L ++ G ARADAL DIQK+G +R+ VP DF PFGSV++DL+ G D Sbjct: 3 MSPRKTMLAVVLGLAAMQGGLARADALADIQKNGVVRIGVPQDFAPFGSVNTDLQLQGLD 62 Query: 61 IDVANLIAKKMGVKVELIPVSSANRIPYLTTKKVDLVISSMGKNAEREKVIDFSAAYAPF 120 IDVA LIA+KMGVK++L+PV+SANRI YL T K DLVIS++G+NAEREKVIDFS YAP+ Sbjct: 63 IDVARLIAQKMGVKLQLVPVASANRIAYLQTHKADLVISTLGRNAEREKVIDFSQPYAPY 122 Query: 121 FNGVFGPADLPVKSAADLAGKTIAVTRGSVEDLELTKIVPASATVKRYEDNNSTISSFLS 180 N VFG A + V ADLA KT+ V RG+ +DL+LT P SAT+KRYEDNN+ IS+++S Sbjct: 123 NNSVFGVASVKVSGPADLADKTVGVARGTFQDLQLTDTAPKSATIKRYEDNNAMISAYVS 182 Query: 181 GQVQLVATGNVVAAAIIAKNPPKKPETKFLIKDSPCFIGLNKGEKKLQDKVDAILADAKK 240 GQVQLV TG+ VA A+ K P KP K++I++S C +GLNKGE L KV+ L AKK Sbjct: 183 GQVQLVGTGDFVAYALAEKAPNNKPVLKYIIQESACNVGLNKGEPALLAKVNEALTAAKK 242 Query: 241 DGSLNAISQKWLGLPLPAGL 260 G LN I QKWL +PLPA + Sbjct: 243 SGELNGIVQKWLNVPLPAKM 262 >gi|300310886|ref|YP_003774978.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 265 Score = 273 bits (698), Expect = 1e-74, Method: Compositional matrix adjust. Identities = 135/257 (52%), Positives = 173/257 (67%) Query: 1 MKLTKLLLGLMAGAMMFSASAARADALDDIQKHGTLRVAVPADFPPFGSVDSDLKPLGFD 60 M K + G M + A AD L DIQK G +++ VP DF P+GSV SDL+ G D Sbjct: 1 MNFIKTAIAASLGLMAVAVHPAMADTLADIQKAGVVKIGVPQDFAPYGSVTSDLQLQGLD 60 Query: 61 IDVANLIAKKMGVKVELIPVSSANRIPYLTTKKVDLVISSMGKNAEREKVIDFSAAYAPF 120 IDVA L+AK MGVK EL+PV ++R+ +L T K+DL+IS++GKNAEREKVIDFS YAP+ Sbjct: 61 IDVARLVAKGMGVKAELVPVPISSRMAFLQTHKIDLIISTLGKNAEREKVIDFSQGYAPY 120 Query: 121 FNGVFGPADLPVKSAADLAGKTIAVTRGSVEDLELTKIVPASATVKRYEDNNSTISSFLS 180 N VFG A + V ADLA +T+ V R + EDL LT VP S +KRYEDNN I++++S Sbjct: 121 NNSVFGVAAIKVAGPADLANQTVGVARSTFEDLMLTDSVPKSTVIKRYEDNNGLIAAYVS 180 Query: 181 GQVQLVATGNVVAAAIIAKNPPKKPETKFLIKDSPCFIGLNKGEKKLQDKVDAILADAKK 240 GQVQLV TG+ VA A+ K P KP K++I S C++G+NKGE L KV+ L +KK Sbjct: 181 GQVQLVGTGDFVAYALAEKTPANKPLLKYIINVSTCYVGMNKGEPALMAKVNEALTASKK 240 Query: 241 DGSLNAISQKWLGLPLP 257 G LN I +KWL +PLP Sbjct: 241 SGELNGIVKKWLNVPLP 257 >gi|300309582|ref|YP_003773674.1| amino acid ABC transporter/signal transduction system periplasmic binding protein [Herbaspirillum seropedicae SmR1] Length = 269 Score = 182 bits (462), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 94/235 (40%), Positives = 142/235 (60%) Query: 23 RADALDDIQKHGTLRVAVPADFPPFGSVDSDLKPLGFDIDVANLIAKKMGVKVELIPVSS 82 +AD LD I LRVA+ PP+ D L+ +G D++ A +AK G+++E++P + Sbjct: 27 QADQLDTILAAKKLRVAIDLAVPPYSMKDDKLRLIGSDVETAQRMAKDFGLELEVVPTTL 86 Query: 83 ANRIPYLTTKKVDLVISSMGKNAEREKVIDFSAAYAPFFNGVFGPADLPVKSAADLAGKT 142 NR+PYL T K D+VIS+ ER KVIDFS YAP V P + + S ADLAGK Sbjct: 87 TNRVPYLQTNKADIVISAFSITPERAKVIDFSVPYAPILVVVGAPRSMKITSPADLAGKK 146 Query: 143 IAVTRGSVEDLELTKIVPASATVKRYEDNNSTISSFLSGQVQLVATGNVVAAAIIAKNPP 202 I TRG+ D ELTKI P+SAT+ R++D+ +++++ +SGQ + AT ++ + KNP Sbjct: 147 IIATRGTTNDQELTKIAPSSATITRFDDDATSMTAMVSGQADIWATSPMLLKVVNQKNPS 206 Query: 203 KKPETKFLIKDSPCFIGLNKGEKKLQDKVDAILADAKKDGSLNAISQKWLGLPLP 257 + E+K +K + +GL KGE +L++K+D + +G LN I +K+ LP Sbjct: 207 RDIESKLTLKANLLAVGLRKGEPRLKEKIDGWIRTNLSNGELNTIYKKYHEAELP 261 >gi|300313169|ref|YP_003777261.1| amino acid transport periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 268 Score = 175 bits (444), Expect = 3e-45, Method: Compositional matrix adjust. Identities = 93/229 (40%), Positives = 139/229 (60%) Query: 29 DIQKHGTLRVAVPADFPPFGSVDSDLKPLGFDIDVANLIAKKMGVKVELIPVSSANRIPY 88 DI+ + VA+ PPFG ++ L+P G D++ A L+AK +GV++E++ V+ NRIP+ Sbjct: 32 DIKARKKVLVAIDLGAPPFGMTNAKLEPQGSDVETARLLAKDLGVELEIVQVTGPNRIPF 91 Query: 89 LTTKKVDLVISSMGKNAEREKVIDFSAAYAPFFNGVFGPADLPVKSAADLAGKTIAVTRG 148 L T K D+VISS ER+KV+ F+ Y V P +KS DL GK +AV RG Sbjct: 92 LMTGKADMVISSFSITPERQKVVAFTQPYGASQFVVAAPKGENIKSLNDLVGKRVAVVRG 151 Query: 149 SVEDLELTKIVPASATVKRYEDNNSTISSFLSGQVQLVATGNVVAAAIIAKNPPKKPETK 208 +++D LT I P + R++D+ + ++ +SGQV + T N +A+AI +NP K ETK Sbjct: 152 NMQDSLLTPIAPKGTNIVRFDDDATVTAAIISGQVDALCTPNSLASAIAKQNPSKNLETK 211 Query: 209 FLIKDSPCFIGLNKGEKKLQDKVDAILADAKKDGSLNAISQKWLGLPLP 257 F IKD P IGL KGE L+ ++ ++ K+G L I Q+W+G P+P Sbjct: 212 FAIKDIPYAIGLRKGEPALEKWLNEWISANLKNGKLPGIYQQWVGSPMP 260 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.133 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 642,541 Number of extensions: 22910 Number of successful extensions: 119 Number of sequences better than 1.0e-08: 16 Number of HSP's gapped: 104 Number of HSP's successfully gapped: 16 Length of query: 260 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 177 Effective length of database: 1,226,865 Effective search space: 217155105 Effective search space used: 217155105 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)