BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0086.p (215 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313540|ref|YP_003777632.1| transcription regulator protein... 427 e-121 gi|300313534|ref|YP_003777626.1| GntR family transcription regul... 113 1e-26 gi|300313824|ref|YP_003777916.1| GntR family transcription regul... 106 2e-24 gi|300309587|ref|YP_003773679.1| GntR family transcription regul... 65 4e-12 >gi|300313540|ref|YP_003777632.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 245 Score = 427 bits (1097), Expect = e-121, Method: Compositional matrix adjust. Identities = 206/215 (95%), Positives = 211/215 (98%) Query: 1 MYQDIYDAIMEHRLPPRTKLTEQTLCQIYDTARHTVRKVLSRLASEGMVDLEANRGAFIA 60 MYQDIYDAIMEHRLPPRTKLTEQTLCQIYDTARHTVRKVL+RLA+EGMVDLEANRGAFIA Sbjct: 31 MYQDIYDAIMEHRLPPRTKLTEQTLCQIYDTARHTVRKVLARLAAEGMVDLEANRGAFIA 90 Query: 61 SPSHAEVRDMFELRNILEHAVLEKVGREAGTRQIAGLRRMVEEERDCYLNGDRPRWIRLS 120 SPS AEVRDMFELRNILEHAVLEK+G AGTRQIAGLRRMVEEERDCYLNGDRPRWIRLS Sbjct: 91 SPSPAEVRDMFELRNILEHAVLEKLGHTAGTRQIAGLRRMVEEERDCYLNGDRPRWIRLS 150 Query: 121 AQFHLALAELTGNALLVETLRKLVSRTTLMIAKTEAPGHNACSFDEHETVLEALEKGDVA 180 AQFHLALAELTGNALLVETLRKLVSRTTLMIAKTEAPGHNACSFDEHETVLEALEKGD+ Sbjct: 151 AQFHLALAELTGNALLVETLRKLVSRTTLMIAKTEAPGHNACSFDEHETVLEALEKGDIK 210 Query: 181 AAQSHMAHHLHLCEDRVRPDADDQFDLRAVLGKGL 215 AAQ+HMAHHLHLCEDRVRPDADDQFDLRAVLGKGL Sbjct: 211 AAQAHMAHHLHLCEDRVRPDADDQFDLRAVLGKGL 245 >gi|300313534|ref|YP_003777626.1| GntR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 249 Score = 113 bits (282), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 71/194 (36%), Positives = 105/194 (54%), Gaps = 5/194 (2%) Query: 5 IYDAIMEHRLPPRTKLTEQTLCQIYDTARHTVRKVLSRLASEGMVDLEANRGAFIASPSH 64 I +AI+ +L P T+L EQ L ++ +R VR+ L+ L + G+V + A RG F+ P+ Sbjct: 17 ITEAILAKQLAPGTRLGEQQLADLFKVSRTQVREALTHLMARGIVTVSARRGWFVIEPTP 76 Query: 65 AEVRDMFELRNILEHAVLEKVG-REAGTRQIAGLRRMVEEERDCYLNGDRPRWIRLSAQF 123 A+ R+ FE R ++E +L + R G I LR + E D L F Sbjct: 77 AQAREAFEARRVIELGLLRQARLRPVGDTAIGQLREHIAREEAAIAGADVGARSYLLGDF 136 Query: 124 HLALAELTGNALLVETLRKLVSRTTL--MIAKTEAPGHNACSFDEHETVLEALEKGDVAA 181 H+ LAE GN+LL ETLR L +RTTL M+ ++ ++CS EH ++EALE+GD+ Sbjct: 137 HVCLAECLGNSLLAETLRDLTARTTLTAMLHQSSEQAEDSCS--EHVLIVEALERGDLEQ 194 Query: 182 AQSHMAHHLHLCED 195 A+ M HL E Sbjct: 195 AEQLMHDHLQHVES 208 >gi|300313824|ref|YP_003777916.1| GntR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 228 Score = 106 bits (264), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 63/190 (33%), Positives = 96/190 (50%) Query: 1 MYQDIYDAIMEHRLPPRTKLTEQTLCQIYDTARHTVRKVLSRLASEGMVDLEANRGAFIA 60 + DI I RLPP KL E+ L ++Y +R +R L LA + +VDL ++GAF++ Sbjct: 14 IASDIAANIAAQRLPPGAKLPEEALARVYQVSRTKIRAALVMLAKDKLVDLIPDKGAFVS 73 Query: 61 SPSHAEVRDMFELRNILEHAVLEKVGREAGTRQIAGLRRMVEEERDCYLNGDRPRWIRLS 120 P+ E R++F +R LE A++ + A A L +E ER + + L Sbjct: 74 KPTVEETRNIFFVRRTLESAMVREFVARATPEDYARLEAHLELERQAVSDDNMQVRSTLL 133 Query: 121 AQFHLALAELTGNALLVETLRKLVSRTTLMIAKTEAPGHNACSFDEHETVLEALEKGDVA 180 + FH+ LA + GN +L + L KL R+ L+ ++ ACS +EH + A GD Sbjct: 134 SDFHILLARIVGNDVLTDILEKLAGRSALITMLYQSTRDAACSSEEHRAFIAAARAGDTE 193 Query: 181 AAQSHMAHHL 190 A M HHL Sbjct: 194 RAMELMEHHL 203 >gi|300309587|ref|YP_003773679.1| GntR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 249 Score = 65.1 bits (157), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 64/217 (29%), Positives = 95/217 (43%), Gaps = 30/217 (13%) Query: 1 MYQDIYDAIMEHRLPPRTKLTEQTLCQIYDTARHTVRKVLSRLASEGMVDLEANRGAFI- 59 MYQ + + + RLPP L EQ +R ++R+ L L EG V++ G ++ Sbjct: 34 MYQPGFTS--QGRLPPERALAEQ-----LGVSRPSIREALVALELEGRVEIRMGSGVYLT 86 Query: 60 --ASPSHAE-----------VRDMFELRNILEHAVLEKVGREAGTRQIAGLRRMVEE-ER 105 A P E + D+ R I E A++ V A R I LR + + ER Sbjct: 87 QAAPPGQQEEPAPDKEMGNSLSDIMGARAIFEGAIVALVAPLAKPRDIKALRAIYDTMER 146 Query: 106 DCYLNGDRPRWIRLSAQFHLALAELTGNALLVETLRKLVS-RTTLMIAK----TEAPGHN 160 +C G P FH+ +A +TGN +LV+T+ +L R + AK E Sbjct: 147 ECQ-AGQSPAAT--DRDFHIRIARMTGNDVLVQTVARLYDERQDPISAKLLDHYEPEDSW 203 Query: 161 ACSFDEHETVLEALEKGDVAAAQSHMAHHLHLCEDRV 197 + EH +LEA+E D AQ+ M HL + R Sbjct: 204 LTALHEHREILEAIEARDAIQAQAAMQRHLKMSLKRT 240 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.134 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 642,273 Number of extensions: 23779 Number of successful extensions: 105 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 103 Number of HSP's successfully gapped: 11 Length of query: 215 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 134 Effective length of database: 1,236,335 Effective search space: 165668890 Effective search space used: 165668890 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 128 (53.9 bits)