BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0077.p (260 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313548|ref|YP_003777640.1| nitrate/sulfonate/bicarbonate A... 496 e-141 gi|300309803|ref|YP_003773895.1| nitrate/sulfonate/bicarbonate A... 111 6e-26 gi|300312968|ref|YP_003777060.1| nitrate/sulfonate/bicarbonate A... 103 2e-23 gi|300309985|ref|YP_003774077.1| nitrate/sulfonate/bicarbonate A... 92 5e-20 gi|300312684|ref|YP_003776776.1| nitrate/sulfonate/bicarbonate A... 89 4e-19 >gi|300313548|ref|YP_003777640.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 259 Score = 496 bits (1276), Expect = e-141, Method: Compositional matrix adjust. Identities = 252/260 (96%), Positives = 253/260 (97%), Gaps = 1/260 (0%) Query: 1 MKTPFNGKLNRAATTLAPWILLLAILGVWQFICSVWQVSDFIFPSPASILQAMVDYAGPI 60 MKTPF GKLNRAATTLAPW LLL ILGVWQFICSVWQVSDFIFPSP SILQAMVDYAGPI Sbjct: 1 MKTPFQGKLNRAATTLAPWFLLLVILGVWQFICSVWQVSDFIFPSPGSILQAMVDYAGPI 60 Query: 61 TEHALSTFWVTMVGFAIAVAVGVTLGFVIGSSPVLYAAAYPLMTAFNALPKAAFVPVLVV 120 TEHALSTFWVTMVGFAIAV VGVTLGFVIGSSP+LYAAAYPLMTAFNALPKAAFVPVLVV Sbjct: 61 TEHALSTFWVTMVGFAIAVVVGVTLGFVIGSSPMLYAAAYPLMTAFNALPKAAFVPVLVV 120 Query: 121 WFGIGAGPAILTAFLISFFPIMVNIATGLATLEPELEDVLRVLGARRMDILLKVGLPRSM 180 WFGIGAGPAILTAFLISFFPIMVNIATGLATLEPELEDVLRVLGARRMDILLKVGLPRSM Sbjct: 121 WFGIGAGPAILTAFLISFFPIMVNIATGLATLEPELEDVLRVLGARRMDILLKVGLPRSM 180 Query: 181 PYFFASLKVAITLAFVGSTVSEMNASNQGIGYLLVSAGSSMKMPLAFAGLVVIGAXCAMA 240 PYFFASLKVAITLAFVGSTVSEMNASNQGIGYLLVSAGSSMKMPLAFAGLVVIGA AMA Sbjct: 181 PYFFASLKVAITLAFVGSTVSEMNASNQGIGYLLVSAGSSMKMPLAFAGLVVIGA-MAMA 239 Query: 241 MYELFAIIEKRTTGWAHRGK 260 MYELFAIIEKRTTGWAHRGK Sbjct: 240 MYELFAIIEKRTTGWAHRGK 259 >gi|300309803|ref|YP_003773895.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 267 Score = 111 bits (277), Expect = 6e-26, Method: Compositional matrix adjust. Identities = 69/248 (27%), Positives = 128/248 (51%), Gaps = 5/248 (2%) Query: 17 APWILLLAILGVWQFICSVWQVSDFIFPSPASILQAMVDYAGPITEHALSTFWVTMVGFA 76 AP I+ +A+L +WQ +C++ +V ++ P+PA I++ + + L T +T + FA Sbjct: 19 APLIIGIALLLMWQIVCTLLEVPPYLVPTPALIVKTLAADWSMLMASLLVTLKITFLAFA 78 Query: 77 IAVAVGVTLGFVIGSSPVLYAAAYPLMTAFNALPKAAFVPVLVVWFGIGAGPAILTAFLI 136 +AV +GV + FV S + + +P P A P++++W + A ++ Sbjct: 79 LAVVLGVAIAFVFVQSRFIEISLFPYAIILQVTPIVAIAPLIIIWVKDTTAALTVCATIM 138 Query: 137 SFFPIMVNIATGLATLEPELEDVLRVLGARRMDILLKVGLPRSMPYFFASLKVAITLAFV 196 + FPI+ N GL ++ P L ++ R+ A R L ++ +P ++PYFF L+++ LA + Sbjct: 139 ALFPIISNTTLGLRSVNPGLLNLFRLNKATRWQTLRRLRIPSALPYFFGGLRISSGLALI 198 Query: 197 GSTVSEMNASNQGIG----YLLVSAGSSMKMPLAFAGLVVIGAXCAMAMYELFAIIEKRT 252 G+ V+E A G G Y ++ AG + +P FA L++I + + + A+ Sbjct: 199 GAVVAEFVAGTGGTGSGLAYQILQAGFQLNIPRLFAALLLITLTGILLFWLMSALSRAML 258 Query: 253 TGWAHRGK 260 GW H + Sbjct: 259 AGW-HESE 265 >gi|300312968|ref|YP_003777060.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 283 Score = 103 bits (256), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 63/205 (30%), Positives = 103/205 (50%), Gaps = 1/205 (0%) Query: 54 VDYAGPITEHALSTFWVTMVGFAIAVAVGVTLGFVIGSSPVLYAAAYPLMTAFNALPKAA 113 + + I H L T T++ F I G+ +G + SP A P + A N++P+ Sbjct: 73 LSFPAEIYSHLLITLTETVLAFVIGTVFGLAIGLWLALSPTASAILDPYVKAANSMPRVI 132 Query: 114 FVPVLVVWFGIGAGPAILTAFLISFFPIMVNIATGLATLEPELEDVLRVLGARRMDILLK 173 P+ +WFG+G + A + FF + N+ G+ + P + R+LGA +L Sbjct: 133 LAPIFAMWFGLGIWSKVALAVTLVFFIVFFNVYQGVKEVSPVVLANARMLGANARQLLRT 192 Query: 174 VGLPRSMPYFFASLKVAITLAFVGSTVSEMNASNQGIGYLLVSAGSSMKMPLAFAGLVVI 233 V LP + + F+SL +I LAFVG+ V E S +G+GYL++ A + + FAG+VV+ Sbjct: 193 VYLPSATSWVFSSLHTSIGLAFVGAVVGEYLGSARGVGYLILQAEGTFDINTVFAGIVVL 252 Query: 234 GAXCAMAMYELFAIIEKRTTGWAHR 258 A+ + +IEKR W + Sbjct: 253 -TIFALVLDLAVGVIEKRLMKWQPK 276 >gi|300309985|ref|YP_003774077.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 278 Score = 91.7 bits (226), Expect = 5e-20, Method: Compositional matrix adjust. Identities = 65/239 (27%), Positives = 118/239 (49%), Gaps = 3/239 (1%) Query: 19 WILLLAILGVWQFICSVWQVSDFIFPSPASILQAMVDYA--GPITEHALSTFWVTMVGFA 76 WI+ LA L + + + + + P P+ + + A G + H ++ GF Sbjct: 29 WIVPLAFLLLVEAGARLELIPARLLPPPSELAHTLWSLAQRGELLPHIAASLARVAAGFV 88 Query: 77 IAVAVGVTLGFVIGSSPVLYAAAYPLMTAFNALPKAAFVPVLVVWFGIGAGPAILTAFLI 136 I A+ V LG ++G S A P A A+P A++P+L++W GI P ++ + Sbjct: 89 IGSALAVALGLLVGISRRAEALLEPSFQALRAIPSLAWIPLLLLWLGIDETPKVVLIAIG 148 Query: 137 SFFPIMVNIATGLATLEPELEDVLRVLGARRMDILLKVGLPRSMPYFFASLKVAITLAFV 196 +FFP+ +N+ G+ ++ +L +V G ++ + LP S+PY FA L+ ++LA++ Sbjct: 149 AFFPVYLNLVAGIRDIDHKLIEVGTTSGLNTQALIGHILLPASLPYLFAGLRGGLSLAWM 208 Query: 197 GSTVSEMNASNQGIGYLLVSAGSSMKMPLAFAGLVVIGAXCAMAMYELFAIIEKRTTGW 255 +E+ A+ +G+GYLL S G P ++I A L +E+++ GW Sbjct: 209 FLVAAELIAATKGVGYLL-SDGRESSRPDLVLAAILILALLGKLSDGLLRAVEQKSLGW 266 >gi|300312684|ref|YP_003776776.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 284 Score = 89.0 bits (219), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 66/241 (27%), Positives = 115/241 (47%), Gaps = 7/241 (2%) Query: 21 LLLAILGVWQFICSVWQVSDFIFPSPASILQAMVDY------AGPITEHALSTFWVTMVG 74 +L+ +LG W+ + + F F P+ I+ + D+ GP+ L T T++G Sbjct: 40 ILVIVLGGWEVCARIGWIDPFFFSQPSLIVAQIYDWMVEGTSQGPLWTQVLVTLEETVLG 99 Query: 75 FAIAVAVGVTLGFVIGSSPVLYAAAYPLMTAFNALPKAAFVPVLVVWFGIGAGPAILTAF 134 F I GV G V+G + +L + N++P+ + V+ FG+G + A Sbjct: 100 FLIGSVAGVIAGIVLGRNKLLSDVFSLYIQIANSIPRVVLGSIFVIAFGLGMASKVALAV 159 Query: 135 LISFFPIMVNIATGLATLEPELEDVLRVLGARRMDILLKVGLPRSMPYFFASLKVAITLA 194 ++ FF + N G+ + + ++LGA R + + V +P ++ + ASL V+ A Sbjct: 160 VMVFFVVFANAFQGVREADRYMIANAQILGASRRQVTMAVVIPSALSWILASLHVSFGFA 219 Query: 195 FVGSTVSEMNASNQGIGYLLVSAGSSMKMPLAFAGLVVIGAXCAMAMYELFAIIEKRTTG 254 VG+ V E S QGIG L+ +A + FA ++V+ A A+A L +E+R Sbjct: 220 LVGAVVGEFLGSKQGIGLLISTAQGAFNASGVFAAMIVL-AVVALAADWLLHALERRLLK 278 Query: 255 W 255 W Sbjct: 279 W 279 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.139 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 818,987 Number of extensions: 34239 Number of successful extensions: 204 Number of sequences better than 1.0e-08: 16 Number of HSP's gapped: 195 Number of HSP's successfully gapped: 16 Length of query: 260 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 177 Effective length of database: 1,226,865 Effective search space: 217155105 Effective search space used: 217155105 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 129 (54.3 bits)