BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0076.p (482 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313549|ref|YP_003777641.1| ABC transporter ATPase [Herbasp... 931 0.0 gi|300310741|ref|YP_003774833.1| ABC transporter ATPase [Herbasp... 262 5e-71 gi|300310063|ref|YP_003774155.1| sugar ABC transporter ATPase [H... 259 3e-70 gi|300310382|ref|YP_003774474.1| sugar ABC transporter ATPase [H... 256 2e-69 gi|300310371|ref|YP_003774463.1| sugar ABC transporter ATPase [H... 239 4e-64 >gi|300313549|ref|YP_003777641.1| ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 518 Score = 931 bits (2407), Expect = 0.0, Method: Compositional matrix adjust. Identities = 470/506 (92%), Positives = 475/506 (93%), Gaps = 24/506 (4%) Query: 1 VRKAYPGVLANDDVSLAVAPGEIHAVLGENGAGKSTLMKIIFGAVKPDAGELRWNGETVQ 60 VRKAYPGVLANDDVSLAVAPGEIHAVLGENGAGKSTLMKIIFGAVKPDAGE+RWNGE VQ Sbjct: 13 VRKAYPGVLANDDVSLAVAPGEIHAVLGENGAGKSTLMKIIFGAVKPDAGEIRWNGEAVQ 72 Query: 61 IANPQVARKLGIAMVFQHFSLFDTLTVTENIALGLDAKTDLKELAERITRTGEKYGLELE 120 IANPQVARKLGIAMVFQHFSLFDTLTVTENIALGLDAKTDLKELAERITRTGEKYGLELE Sbjct: 73 IANPQVARKLGIAMVFQHFSLFDTLTVTENIALGLDAKTDLKELAERITRTGEKYGLELE 132 Query: 121 PQRHVHTLSVGERQRVEIVRALLTEPQLLILDEPTSVLTPQAVEKLFVTLRRLADEGCSI 180 PQRHVHTLSVGERQRVEIVRALLTEPQLLILDEPTSVLTPQAVEKLFVTLRRLADEGCSI Sbjct: 133 PQRHVHTLSVGERQRVEIVRALLTEPQLLILDEPTSVLTPQAVEKLFVTLRRLADEGCSI 192 Query: 181 LYISHKLDEIRALCHSATVMRGGRVTGNCDPRNESSASLSRMMIGDELVRLQRERNPDSL 240 LYISHKLDEIRALCHSATVMRGGRVTGNCDPRNESSASLSRMMIGDELVRLQRERNP+S+ Sbjct: 193 LYISHKLDEIRALCHSATVMRGGRVTGNCDPRNESSASLSRMMIGDELVRLQRERNPESM 252 Query: 241 RNERKLHVNRLSLPKAHLFATELKDIECEVRAGEIVGIAGVSGNGQQELLAALSG----- 295 RNERKLHVNRLSLPKAHLFATELKDIECEVRAGEIVGIAGVSGNGQQELLAALSG Sbjct: 253 RNERKLHVNRLSLPKAHLFATELKDIECEVRAGEIVGIAGVSGNGQQELLAALSGEDQRA 312 Query: 296 ---VIT--------------RAAGLGLVPEERLGRGAVPGLSLTANMLLSHQKAPYVKRG 338 +I RAAGLGLVPEERLGRGAVP LSLTANMLLSHQKAPYVKRG Sbjct: 313 APHMIMLGSKAVGDLSPNPRRAAGLGLVPEERLGRGAVPTLSLTANMLLSHQKAPYVKRG 372 Query: 339 MIDFGFTRRAAASIIERFKVKAAGPDAPAKSLSGGNLQKFIVGREMERKPAVFVVAQPTW 398 MIDFGFTRRAAASIIERFKVKAAGPDAPAKSLSGGNLQKFIVGREMERKPAVFVVAQPTW Sbjct: 373 MIDFGFTRRAAASIIERFKVKAAGPDAPAKSLSGGNLQKFIVGREMERKPAVFVVAQPTW 432 Query: 399 GVDVGAAAQIHGEILKLKQEGCAVLVISEELDELFALCDRLHVIAKGRLSPSIPIEQATR 458 GVDVGAAAQIHGEILKLKQEGCAVLVISEELDELFALCDRLHVIAKGRLSPSIPIEQATR Sbjct: 433 GVDVGAAAQIHGEILKLKQEGCAVLVISEELDELFALCDRLHVIAKGRLSPSIPIEQATR 492 Query: 459 EQVGVWMSGLWDEPAA--AAAEVNHG 482 EQVGVWMSGLWDEPAA EV +G Sbjct: 493 EQVGVWMSGLWDEPAALDKGTEVGNG 518 >gi|300310741|ref|YP_003774833.1| ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 522 Score = 262 bits (669), Expect = 5e-71, Method: Compositional matrix adjust. Identities = 182/506 (35%), Positives = 271/506 (53%), Gaps = 48/506 (9%) Query: 3 KAYPGVLANDDVSLAVAPGEIHAVLGENGAGKSTLMKIIFGAVKPDAGELRWNGETVQIA 62 K++ A D+VSL + G IHA+LGENGAGKSTL+K + G D G + + V I Sbjct: 13 KSFGSFRALDEVSLKIRAGTIHALLGENGAGKSTLVKGLVGYSPLDQGSILADRREVDIR 72 Query: 63 NPQVARKLGIAMVFQHFSLFDTLTVTENIALGLDAKTDLKELAERITRTGEKYGLE---- 118 + +V +LGI MV+QHF+L +LTV EN+ L A+ DL RI + E+ LE Sbjct: 73 SARVPNQLGIGMVYQHFTLAPSLTVAENLLL---ARGDLP---WRIRWSSERAVLEEFMA 126 Query: 119 -----LEPQRHVHTLSVGERQRVEIVRALLTEPQLLILDEPTSVLTPQAVEKLFVTLRRL 173 L P+R V +LS GE+Q++EI++ L + LILDEPTSVLTPQ +++ + L Sbjct: 127 KMPFKLSPERTVSSLSAGEKQKLEILKQLYLRRRFLILDEPTSVLTPQEADEVLGLMHAL 186 Query: 174 A-DEGCSILYISHKLDEIRALCHSATVMRGGRVTGNCDPRNESSASLSRMMIGDELV-RL 231 + ++L I+HK E+ A TV+R GR+ G+ S L+ M+G + Sbjct: 187 TRQQELTVLMITHKFHEVSAYADDVTVLRKGRLVGSARVAETSPDMLAHWMMGQARAEKA 246 Query: 232 QRERNPDSLRNERKLHVNRLSLPKAHLFATELKDIECEVRAGEIVGIAGVSGNGQQELLA 291 Q R ++ L V L++ A ++ + +VR GEIVG+AG+SGNGQ+EL+ Sbjct: 247 QVARPAVPVQASVGLEVRDLTVNNDRGVAA-VRALSLQVRRGEIVGLAGISGNGQKELVE 305 Query: 292 ALSGVI--------------------TRAAGLGLVPEERLGRGAVPGLSLTANMLLSHQK 331 AL G R + +PEE L + G+S+ NM L + Sbjct: 306 ALLGQRRLLLGEIRVEGAPYAATREEMRRLRVFALPEEPLRNACIAGMSVAENMALRNFD 365 Query: 332 APYVKRG--MIDFGFTRRAAASIIERFKVKAAGPDAPAKSLSGGNLQKFIVGREM----- 384 KRG ID +R A ++I F VK P+ P +LSGGN+Q+ ++ RE+ Sbjct: 366 VAPFKRGGWRIDRSAMKRQAQALISAFNVKPPVPERPIGTLSGGNVQRAVLARELGEDGQ 425 Query: 385 ERKPAVFVVAQPTWGVDVGAAAQIHGEILKLKQEGCAVLVISEELDELFALCDRLHVIAK 444 + V +VA P +G+D + A IH +L+ + G AVL++SE+LDEL L DR+ V+ + Sbjct: 426 DGAANVLIVANPVFGLDFASVADIHARLLQARARGAAVLLVSEDLDELLELSDRILVMTE 485 Query: 445 GRL---SPSIPIEQATREQVGVWMSG 467 GR+ + + E A R +G WM+G Sbjct: 486 GRIVHVAQDVAKEGADRAALGRWMAG 511 >gi|300310063|ref|YP_003774155.1| sugar ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 521 Score = 259 bits (663), Expect = 3e-70, Method: Compositional matrix adjust. Identities = 163/493 (33%), Positives = 265/493 (53%), Gaps = 43/493 (8%) Query: 1 VRKAYPGVLANDDVSLAVAPGEIHAVLGENGAGKSTLMKIIFGAVKPDAGELRWNGETVQ 60 +RK++ LA D+ L + PGEIHA++GENGAGKSTLMK++ G PD GE+ +G V Sbjct: 12 IRKSFGATLALSDMHLTIRPGEIHALMGENGAGKSTLMKVLSGVHAPDQGEILLDGRPVA 71 Query: 61 IANPQVARKLGIAMVFQHFSLFDTLTVTENIALGLDAKT-----DLKELAERITRTGEKY 115 + +P +R GI +++Q ++ ++V N+ +G + +T D + R + Sbjct: 72 LRDPGASRAAGINLIYQELAVAPNISVAANVFMGSELRTRLGLIDHAAMRSRTDAVLRQL 131 Query: 116 GLELEPQRHVHTLSVGERQRVEIVRALLTEPQLLILDEPTSVLTPQAVEKLFVTLRRLAD 175 G LS+ E+Q+VEI RAL+ +++I+DEPT+ L+ + E+LF +RRL D Sbjct: 132 GAGFGASDLAGRLSIAEQQQVEIARALVHRSRIVIMDEPTAALSERETEQLFNVVRRLRD 191 Query: 176 EGCSILYISHKLDEIRALCHSATVMRGGRVTGNCDPRNESSASLSRMMIG---DELVRLQ 232 EG +I+YISH++ E+ AL TV+R G G S + +MM+G E + Q Sbjct: 192 EGLAIIYISHRMAEVYALADRVTVLRDGSFVGELVRDEIDSERIVQMMVGRSLSEFYQHQ 251 Query: 233 RERNPDSLRNERKLHVNRLSLPKAHLFATELKDIECEVRAGEIVGIAGVSGNGQQELLAA 292 R D+ + + V L+ +++ +VRAGE++G AG+ G G+ EL Sbjct: 252 RIAPADAAQLPTVMQVRALA-------GGKIRPASFDVRAGEVLGFAGLVGAGRTELARL 304 Query: 293 LSGVITRA---------------------AGLGLVPEERLGRGAVPGLSLTANMLLSHQK 331 L G R+ AG+ VPE+R G+G +++ AN + + Sbjct: 305 LFGADPRSGGDILLEGRPVHIDQPRAAMRAGIAYVPEDRKGQGLFLQMAVAANATM-NVA 363 Query: 332 APYVKRGMI---DFGFTRRAAASIIERFKVKAAGPDAPAKSLSGGNLQKFIVGREMERKP 388 + + + G++ G RAA I+R VK A P+ P LSGGN QK ++ R +E P Sbjct: 364 SRHTRLGLVRSRSLGGVARAA---IQRLNVKVAHPETPVGKLSGGNQQKVLLARWLEIAP 420 Query: 389 AVFVVAQPTWGVDVGAAAQIHGEILKLKQEGCAVLVISEELDELFALCDRLHVIAKGRLS 448 V ++ +PT GVD+ A ++I+ + +L +G AV+VIS EL E+ +CDR+ V+ +G ++ Sbjct: 421 KVLILDEPTRGVDIYAKSEIYQLVHRLASQGVAVVVISSELPEVIGICDRVLVMREGMIT 480 Query: 449 PSIPIEQATREQV 461 + T+E + Sbjct: 481 GELAGAAITQENI 493 Score = 66.2 bits (160), Expect = 6e-12, Method: Compositional matrix adjust. Identities = 53/226 (23%), Positives = 99/226 (43%), Gaps = 27/226 (11%) Query: 263 LKDIECEVRAGEIVGIAGVSGNGQQELLAALSGV---------------------ITRAA 301 L D+ +R GEI + G +G G+ L+ LSGV +RAA Sbjct: 22 LSDMHLTIRPGEIHALMGENGAGKSTLMKVLSGVHAPDQGEILLDGRPVALRDPGASRAA 81 Query: 302 GLGLVPEERLGRGAVPGLSLTANMLLSHQKAPYVKRGMIDFGFTRRAAASIIERFKVKAA 361 G+ L+ +E P +S+ AN+ + + + G+ID R +++ + Sbjct: 82 GINLIYQEL---AVAPNISVAANVFMGSEL--RTRLGLIDHAAMRSRTDAVLRQLGAGFG 136 Query: 362 GPDAPAKSLSGGNLQKFIVGREMERKPAVFVVAQPTWGVDVGAAAQIHGEILKLKQEGCA 421 D A LS Q+ + R + + + ++ +PT + Q+ + +L+ EG A Sbjct: 137 ASDL-AGRLSIAEQQQVEIARALVHRSRIVIMDEPTAALSERETEQLFNVVRRLRDEGLA 195 Query: 422 VLVISEELDELFALCDRLHVIAKGRLSPSIPIEQATREQVGVWMSG 467 ++ IS + E++AL DR+ V+ G + ++ E++ M G Sbjct: 196 IIYISHRMAEVYALADRVTVLRDGSFVGELVRDEIDSERIVQMMVG 241 >gi|300310382|ref|YP_003774474.1| sugar ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 520 Score = 256 bits (655), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 161/488 (32%), Positives = 267/488 (54%), Gaps = 35/488 (7%) Query: 1 VRKAYPGVLANDDVSLAVAPGEIHAVLGENGAGKSTLMKIIFGAVKPDAGELRWNGETVQ 60 V K +PGVLA D+ +A GE+HA++GENGAGKSTLMKI+ G + D+G++ +G+ V+ Sbjct: 28 VCKRFPGVLALDNCQFELAAGEVHALMGENGAGKSTLMKILSGVYQRDSGDILLDGKPVE 87 Query: 61 IANPQVARKLGIAMVFQHFSLFDTLTVTENIALGLDAK------TDLKELAERITRTGEK 114 I P+ A+ LGI ++ Q +L + L+ +NI +G + + D EL + + Sbjct: 88 ITEPRQAQALGIGIIHQELNLMNHLSAAQNIFIGREPRKAMGLFIDEDELNRQAAAIFAR 147 Query: 115 YGLELEPQRHVHTLSVGERQRVEIVRALLTEPQLLILDEPTSVLTPQAVEKLFVTLRRLA 174 L+++P V L+V +Q VEI +AL + ++LI+DEPT+ L + +LF +R L Sbjct: 148 MRLDMDPSTPVGELTVARQQMVEIAKALSFDSRVLIMDEPTAALNNAEIAELFRIIRDLQ 207 Query: 175 DEGCSILYISHKLDEIRALCHSATVMRGGRVTGNCDPRNESSASLSRMMIGDELVRLQRE 234 +G I+YISHK+DE+R + +VMR G+ + S ++ MM+G L QR Sbjct: 208 AQGVGIVYISHKMDELRQIADRVSVMRDGKYIATVPMQETSMDTIISMMVGRALDGEQR- 266 Query: 235 RNPDSLRNERKLHVNRLSLPKAHLFATELKDIECEVRAGEIVGIAGVSGNGQQELLAALS 294 PD+ RN+ L V L+ +A ++D+ +R GEI+G AG+ G G+ E+ A+ Sbjct: 267 IPPDTSRNDVVLEVRGLNRGRA------IRDVSFTLRKGEILGFAGLMGAGRTEVARAIF 320 Query: 295 G----------------VITRAA-----GLGLVPEERLGRGAVPGLSLTANMLLSHQKAP 333 G VI A G+G + E+R G G+ + AN+ LS Sbjct: 321 GADPLEAGEIIIHGGKAVIKSPADAVAHGIGYLSEDRKHFGLAVGMDVQANIALSSM-GR 379 Query: 334 YVKRGMIDFGFTRRAAASIIERFKVKAAGPDAPAKSLSGGNLQKFIVGREMERKPAVFVV 393 + + G +D R AA + + +K + A+ LSGGN QK ++ + + R + Sbjct: 380 FTRVGFMDQRAIREAAQMYVRQLAIKTPSVEQQARLLSGGNQQKIVIAKWLLRDCDILFF 439 Query: 394 AQPTWGVDVGAAAQIHGEILKLKQEGCAVLVISEELDELFALCDRLHVIAKGRLSPSIPI 453 +PT G+DVGA ++I+ + L ++G A+++IS EL E+ + R+ V+ +GR++ + Sbjct: 440 DEPTRGIDVGAKSEIYKLLDALAEQGKAIVMISSELPEVLRMSHRVLVMCEGRITGELAR 499 Query: 454 EQATREQV 461 AT+E++ Sbjct: 500 ADATQEKI 507 Score = 63.9 bits (154), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 54/222 (24%), Positives = 101/222 (45%), Gaps = 18/222 (8%) Query: 263 LKDIECEVRAGEIVGIAGVSGNGQQELLAALSGVITRAAGLGL-------VPEERLGRGA 315 L + + E+ AGE+ + G +G G+ L+ LSGV R +G L + E R + Sbjct: 38 LDNCQFELAAGEVHALMGENGAGKSTLMKILSGVYQRDSGDILLDGKPVEITEPRQAQAL 97 Query: 316 VPGLSLTANMLLSHQKA---------PYVKRGM-IDFGFTRRAAASIIERFKVKAAGPDA 365 G+ L++H A P G+ ID R AA+I R ++ P Sbjct: 98 GIGIIHQELNLMNHLSAAQNIFIGREPRKAMGLFIDEDELNRQAAAIFARMRLDM-DPST 156 Query: 366 PAKSLSGGNLQKFIVGREMERKPAVFVVAQPTWGVDVGAAAQIHGEILKLKQEGCAVLVI 425 P L+ Q + + + V ++ +PT ++ A++ I L+ +G ++ I Sbjct: 157 PVGELTVARQQMVEIAKALSFDSRVLIMDEPTAALNNAEIAELFRIIRDLQAQGVGIVYI 216 Query: 426 SEELDELFALCDRLHVIAKGRLSPSIPIEQATREQVGVWMSG 467 S ++DEL + DR+ V+ G+ ++P+++ + + + M G Sbjct: 217 SHKMDELRQIADRVSVMRDGKYIATVPMQETSMDTIISMMVG 258 >gi|300310371|ref|YP_003774463.1| sugar ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 518 Score = 239 bits (610), Expect = 4e-64, Method: Compositional matrix adjust. Identities = 173/520 (33%), Positives = 273/520 (52%), Gaps = 55/520 (10%) Query: 1 VRKAYPGVLANDDVSLAVAPGEIHAVLGENGAGKSTLMKIIFGAVKPD---AGELRWNGE 57 + K++PGV A ++V+LAV GEIHA++GENGAGKSTLMK++ G V P +G++ + GE Sbjct: 10 IEKSFPGVKALNNVNLAVREGEIHAIVGENGAGKSTLMKVLSG-VYPHGSYSGDIVYKGE 68 Query: 58 TVQIANPQVARKLGIAMVFQHFSLFDTLTVTENIALGLD-AKTDLKELAERITRTGE--- 113 + + + LGI ++ Q +L L+V EN+ LG + A+ + + + R E Sbjct: 69 VRAFKDIRDSEHLGIIIIHQELALVPLLSVMENLFLGNEQARGGVIDWEQSYVRAKELLA 128 Query: 114 KYGLELEPQRHVHTLSVGERQRVEIVRALLTEPQLLILDEPTSVLTPQAVEKLFVTLRRL 173 K GL+ P V L VG++Q +EI +AL E +LLILDEPT+ L + L L L Sbjct: 129 KVGLKESPLTQVGDLGVGKQQLIEIAKALSKEVKLLILDEPTASLNESDSDALLELLLEL 188 Query: 174 ADEGCSILYISHKLDEIRALCHSATVMRGGRVTGNCDPRNE--SSASLSRMMIGDELVRL 231 +G + + ISHKL+EI + S TV+R G D R E S + + M+G E+ Sbjct: 189 KRQGIASILISHKLNEISKVADSITVLRDGTTVDTFDCRAEPISEDRIIQHMVGREMADR 248 Query: 232 QRERNPD---------SLRNERKLHVNRLSLPKAHLFATELKDIECEVRAGEIVGIAGVS 282 +R+P R LH +RL++ KD+ VRAGEIVGIAG+ Sbjct: 249 YPQRDPQIGEVIFEVRDWRVHHPLHTDRLAI----------KDVNMSVRAGEIVGIAGLM 298 Query: 283 GNGQQELLAALSG---------------------VITRA--AGLGLVPEERLGRGAVPGL 319 G G+ EL ++ G I +A G+ V E+R G G V Sbjct: 299 GAGRTELAKSIFGRAYGKKITGQAFLHGKEVDLSTIEKAIAKGIAYVTEDRKGDGLVLEE 358 Query: 320 SLTANMLLSHQKAPYVKRGMIDFGFTRRAAASIIERFKVKAAGPDAPAKSLSGGNLQKFI 379 + N+ L++ +R +ID + AA ++ +++ + +LSGGN QK + Sbjct: 359 DIKKNISLANL-GGVSERTVIDEAREYKIAADFKQQMRIRCSSVLQKVVNLSGGNQQKVV 417 Query: 380 VGREMERKPAVFVVAQPTWGVDVGAAAQIHGEILKLKQEGCAVLVISEELDELFALCDRL 439 + + + +P V ++ +PT G+DVGA +I+ I KL EG +++IS E+ EL +CDR+ Sbjct: 418 LSKWLFSQPEVLILDEPTRGIDVGAKFEIYNIISKLAAEGKCIIMISSEMPELLGMCDRI 477 Query: 440 HVIAKGRLSPSIPIEQATREQV--GVWMSGLWDEPAAAAA 477 +V+ +G+ +P +AT+E + + + DEP + A Sbjct: 478 YVMNEGQFVGHLPKAEATQENIMRAIMRNKRIDEPGLSKA 517 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,557,729 Number of extensions: 67826 Number of successful extensions: 857 Number of sequences better than 1.0e-08: 132 Number of HSP's gapped: 552 Number of HSP's successfully gapped: 167 Length of query: 482 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 393 Effective length of database: 1,198,455 Effective search space: 470992815 Effective search space used: 470992815 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)