BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0027.0063.p
         (355 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313560|ref|YP_003777652.1| peptide chain release factor 1 ...   705   0.0  
gi|300312096|ref|YP_003776188.1| peptide chain release factor 2 ...   219   3e-58

>gi|300313560|ref|YP_003777652.1| peptide chain release factor 1
           [Herbaspirillum seropedicae SmR1]
          Length = 359

 Score =  705 bits (1820), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 342/355 (96%), Positives = 350/355 (98%)

Query: 1   MLAKLDQLAERLEELNSLLAQEDATSSMDNFRKMTREHAELGPLVALYHDYVQASEDIRT 60
           MLAKLDQLAERLEELNSLLAQEDAT+SMDNFRKMTREHAELGPLVALYH+YVQA+EDIRT
Sbjct: 5   MLAKLDQLAERLEELNSLLAQEDATASMDNFRKMTREHAELGPLVALYHEYVQAAEDIRT 64

Query: 61  AGELLSDPDMKAFAQEEIDGAKARMEALELDLQKMLLPKDPNDERNIFLEIRAGTGGDES 120
           A ELL+DPDMKAFAQEEIDGAKARME LELDLQKMLLPKDPNDERNIFLEIRAGTGGDES
Sbjct: 65  AQELLADPDMKAFAQEEIDGAKARMEGLELDLQKMLLPKDPNDERNIFLEIRAGTGGDES 124

Query: 121 ALFAGDLLRMYTRYAERQRWQVEIVSASESELGGYKEVIARIAGFGAYSRLKFESGGHRV 180
           ALFAGDLLRMYTRYAERQRWQVEIVSASESELGGYKEVIARI G GAYSRLKFESGGHRV
Sbjct: 125 ALFAGDLLRMYTRYAERQRWQVEIVSASESELGGYKEVIARIVGLGAYSRLKFESGGHRV 184

Query: 181 QRVPATETQGRIHTSACTVAVMPEADEVEDVDINPADLRIDTYRASGAGGQHINKTDSAV 240
           QRVPATETQGRIHTSACTVAVMPEADEV DV+INPAD+RIDTYRASGAGGQHINKTDSAV
Sbjct: 185 QRVPATETQGRIHTSACTVAVMPEADEVGDVEINPADIRIDTYRASGAGGQHINKTDSAV 244

Query: 241 RITHLPTGIVVECQDDRSQHKNKAQAMKVLAARIKDVQLREQQSKEAATRKSLIGSGDRS 300
           RITHLPTGIVVECQDDRSQHKNKAQAMKVLAARIKDVQLR+QQS+EAATRKSLIGSGDRS
Sbjct: 245 RITHLPTGIVVECQDDRSQHKNKAQAMKVLAARIKDVQLRQQQSEEAATRKSLIGSGDRS 304

Query: 301 ERIRTYNFPQGRMTDHRINLTLYKLDFIMDGDLDELTNALITEHQAELLAQLGDD 355
           ERIRTYNFPQGRMTDHRINLTLYKLDFIMDGDLDELTNALITEHQAELLAQLGDD
Sbjct: 305 ERIRTYNFPQGRMTDHRINLTLYKLDFIMDGDLDELTNALITEHQAELLAQLGDD 359


>gi|300312096|ref|YP_003776188.1| peptide chain release factor 2
           [Herbaspirillum seropedicae SmR1]
          Length = 337

 Score =  219 bits (558), Expect = 3e-58,   Method: Compositional matrix adjust.
 Identities = 129/344 (37%), Positives = 202/344 (58%), Gaps = 28/344 (8%)

Query: 9   AERLEELNSLLAQEDATSSMDNFRKMTREHAELGPLVALYHDYVQASEDIRTAGELLSDP 68
           +E+LE++N+ L   +  +     + + +E   L  +V            I+   EL    
Sbjct: 3   SEKLEQVNAELEDPNVWNDQKRAQDLGKEKKSLEAIVHTL---------IKIDSELTDAR 53

Query: 69  DMKAFAQEE--------IDGAKARMEAL--ELDLQKMLLPKDPNDERNIFLEIRAGTGGD 118
           D+   A+EE        I+G    ++ +  E++ ++M     P D  N F++I+AG GG 
Sbjct: 54  DLFEMAREEKDEDTIIAIEGDAENLKKMVEEMEFRRMF--SGPMDANNCFIDIQAGAGGT 111

Query: 119 ESALFAGDLLRMYTRYAERQRWQVEIVSASESELGGYKEVIARIAGFGAYSRLKFESGGH 178
           E+  +A  LLR Y RY ER+ ++ EI+  S+ E+ G K    ++ G  AY  L+ E+G H
Sbjct: 112 EAQDWASMLLRQYLRYCERKGFKAEILEQSDGEVAGIKTATIKVEGEYAYGFLRTETGVH 171

Query: 179 R-VQRVPATETQGRIHTSACTVAVMPEADEVEDVDINPADLRIDTYRASGAGGQHINKTD 237
           R V++ P   + GR HTS  ++ V PE DE  +++INPAD+R+DTYRASGAGGQHINKTD
Sbjct: 172 RLVRKSPFDSSNGR-HTSFSSLFVYPEVDESFEIEINPADVRVDTYRASGAGGQHINKTD 230

Query: 238 SAVRITHLPTGIVVECQDDRSQHKNKAQAMKVLAARIKDVQLREQQSKEAATR--KSLIG 295
           SAVR+TH P+GIVV+CQ+DRSQH+N+A+A  +L A++ +++LR++ S++      K+ +G
Sbjct: 231 SAVRLTHAPSGIVVQCQNDRSQHRNRAEAWDMLRAKLFELELRKRMSEQQKLEDSKTDVG 290

Query: 296 SGDRSERIRTYNFPQGRMTDHRINLTLYKLDFIMDGDLDELTNA 339
            G    +IR+Y   Q R+ D R N        ++DGD+D+   A
Sbjct: 291 WG---HQIRSYVLDQSRIKDLRTNFETGNTKAVLDGDIDDFIAA 331


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.316    0.132    0.364 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,075,987
Number of extensions: 44288
Number of successful extensions: 130
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 129
Number of HSP's successfully gapped: 2
Length of query: 355
Length of database: 1,619,870
Length adjustment: 86
Effective length of query: 269
Effective length of database: 1,212,660
Effective search space: 326205540
Effective search space used: 326205540
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 131 (55.1 bits)