BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0027.0055.p
         (233 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313568|ref|YP_003777660.1| glutathione S-transferase [Herb...   461   e-131
gi|300309973|ref|YP_003774065.1| glutathione S-transferase [Herb...   177   7e-46

>gi|300313568|ref|YP_003777660.1| glutathione S-transferase
           [Herbaspirillum seropedicae SmR1]
          Length = 233

 Score =  461 bits (1187), Expect = e-131,   Method: Compositional matrix adjust.
 Identities = 220/233 (94%), Positives = 229/233 (98%)

Query: 1   MQDTELDPVLSETLQKAGRHMPTLVIANKNYSSWSMRPWVTAVAFDIPFKEVRVLLDQPE 60
           MQDTELDPVLSETLQKAGRH+PTLVIANKNYSSWSMRPWV  VAFDIPFKEVR+LLDQ +
Sbjct: 1   MQDTELDPVLSETLQKAGRHLPTLVIANKNYSSWSMRPWVAMVAFDIPFKEVRLLLDQAD 60

Query: 61  TAGEIARYTGSGRVPVLLDGDIAIWDSLSICEYLAEQFPDRHLWPARTDARAIARSICAE 120
           TA EIARYTGSGRVPVLLDGDIAIWDSLSICEYLAEQFPDRHLWPARTDARAIARSICAE
Sbjct: 61  TAAEIARYTGSGRVPVLLDGDIAIWDSLSICEYLAEQFPDRHLWPARTDARAIARSICAE 120

Query: 121 MHSGFTGLRTAMSMDIRGRYPGKGRTADAQADIARVCEIWEECLSQFGHHEFLFGDFSIA 180
           MHSGFTGLR+AMSMDIRGRYPG+GRTA+AQADIARVCEIWEECLSQFGHH+FLFGDFSIA
Sbjct: 121 MHSGFTGLRSAMSMDIRGRYPGQGRTAEAQADIARVCEIWEECLSQFGHHDFLFGDFSIA 180

Query: 181 DAFYAPVVMRFVSYQVPLAPALQAYADRVREHPAVARWIAEALAEKEILPDHQ 233
           DAFYAPVVMRF+SYQVPLAPALQAYADRVREHPAVARWIAEALAEKEILPDH+
Sbjct: 181 DAFYAPVVMRFISYQVPLAPALQAYADRVREHPAVARWIAEALAEKEILPDHK 233


>gi|300309973|ref|YP_003774065.1| glutathione S-transferase
           [Herbaspirillum seropedicae SmR1]
          Length = 218

 Score =  177 bits (449), Expect = 7e-46,   Method: Compositional matrix adjust.
 Identities = 95/206 (46%), Positives = 126/206 (61%), Gaps = 2/206 (0%)

Query: 23  TLVIANKNYSSWSMRPWVTAVAFDIPFKEVRVLLDQPETAGEIARYTGSGRVPVLLDGDI 82
           TL I +KNYSSWS+R W+ A    + F+E  V  D P    EI   + S  VP L  GD+
Sbjct: 5   TLRITSKNYSSWSLRGWLMARFAGLSFEEDIVPPDDPSARAEILLLSSSILVPSLHHGDV 64

Query: 83  AIWDSLSICEYLAEQFPDRHLWPARTDARAIARSICAEMHSGFTGLRTAMSMDIRGRYPG 142
            +WD+L+I EYL E  P   L PA   ARA  RS+C EMHSGF+ LR A+ M+++G +P 
Sbjct: 65  LVWDTLAIAEYLNEIRPAAKLLPADLAARAHCRSVCGEMHSGFSALRAALPMNLKGHFPN 124

Query: 143 KGRTADAQADIARVCEIWEECLSQFGHHEFLFGD-FSIADAFYAPVVMRFVSYQVPLAPA 201
               + A+ADI R+  IW+ECL+++G   FLFG+  ++ADA YAPVV RF++Y V L P 
Sbjct: 125 FKVWSRAEADIVRITTIWKECLAKYG-GPFLFGEQRTVADAMYAPVVTRFMTYDVKLDPI 183

Query: 202 LQAYADRVREHPAVARWIAEALAEKE 227
             AYA R+   P +  WI  A  E E
Sbjct: 184 CTAYAKRIMAMPEMQEWITAAKKEPE 209


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.323    0.136    0.426 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 804,923
Number of extensions: 33843
Number of successful extensions: 99
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 99
Number of HSP's successfully gapped: 3
Length of query: 233
Length of database: 1,619,870
Length adjustment: 82
Effective length of query: 151
Effective length of database: 1,231,600
Effective search space: 185971600
Effective search space used: 185971600
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 129 (54.3 bits)