BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0041.p (374 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313580|ref|YP_003777672.1| outer membrane porin [Herbaspir... 516 e-147 gi|300309527|ref|YP_003773619.1| outer membrane protein (porin) ... 511 e-146 gi|300310328|ref|YP_003774420.1| porin signal peptide protein [H... 294 1e-80 gi|300312191|ref|YP_003776283.1| outer membrane porin [Herbaspir... 268 6e-73 gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) ... 170 2e-43 >gi|300313580|ref|YP_003777672.1| outer membrane porin [Herbaspirillum seropedicae SmR1] Length = 387 Score = 516 bits (1328), Expect = e-147, Method: Compositional matrix adjust. Identities = 289/387 (74%), Positives = 319/387 (82%), Gaps = 13/387 (3%) Query: 1 MKKSLLALAVLGAFAGAAQAQSSVTIYGIVDTGLVYTSKA--VNAT-TGSTGSKFGLNSG 57 MKKSLLALAVLGAFAGAAQAQSSVTIYGIVDTG+ Y+SK +A +TGSKFG+NSG Sbjct: 1 MKKSLLALAVLGAFAGAAQAQSSVTIYGIVDTGIAYSSKVGYTDANGNAATGSKFGINSG 60 Query: 58 IIQGSRIGFKGVEDXGG-LSAVFQLETGFNNDNGALNGQDA-GSTNLFRRKSVVGLSGGF 115 +IQGSRIGFKGVED GG LSAVF LETGF +D+G L G DA S+NLFRRKSVVGLSGGF Sbjct: 61 VIQGSRIGFKGVEDLGGGLSAVFNLETGFTSDDGGLQGSDAVTSSNLFRRKSVVGLSGGF 120 Query: 116 GTVLAGRQTDFADTISAYTSVNDFGGVTGAVGHNLDRLEGTRTNNSISYTTANLSGFTGN 175 GTVL GRQTDFADTISAYT+V DFGGV G +L+RL+GTRTNNS+SYTTANLSGFTGN Sbjct: 121 GTVLLGRQTDFADTISAYTAVADFGGVVANSGSSLNRLQGTRTNNSVSYTTANLSGFTGN 180 Query: 176 LMYGFGETAGKTSAGQSYGIGGKYDNGPLGLGINYYQSKQGSTPSDTSLISTANGAQ--- 232 L+YGFGE AGKTSAGQ+YGIGGKYDNGPLGLGINYYQSK GSTPSDTSLI A Sbjct: 181 LIYGFGEQAGKTSAGQAYGIGGKYDNGPLGLGINYYQSKAGSTPSDTSLIPAAGATNANT 240 Query: 233 ----AGNTAFKGLNVVASYQFGPARVYGNWSRVKQDLNTSTTLAR-SLGNTTKADVYELG 287 AGNTA K LNVVASYQFGPARVY N+SRVKQDL + T+A +L N KAD YE+G Sbjct: 241 ALGVAGNTALKSLNVVASYQFGPARVYANYSRVKQDLKGTPTVATATLANAQKADTYEIG 300 Query: 288 TAYSLSPSLKLLASVQHTRADFANGGNKGKLTQFNLGTDYWLSKRTDLYAFVSNIRATDM 347 TAYSLSPSLKLLA+V H+RA F KGKLTQ +LG DY+LSKRTDLYAFVSN+RA+D+ Sbjct: 301 TAYSLSPSLKLLAAVDHSRATFNGVNTKGKLTQISLGADYFLSKRTDLYAFVSNMRASDI 360 Query: 348 INTGVYGDTTGSDASQTAVAVGVRHKF 374 N GV G TGSDASQTA+ G+RHKF Sbjct: 361 QNPGVTGGATGSDASQTAIITGIRHKF 387 >gi|300309527|ref|YP_003773619.1| outer membrane protein (porin) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 390 Score = 511 bits (1317), Expect = e-146, Method: Compositional matrix adjust. Identities = 285/393 (72%), Positives = 317/393 (80%), Gaps = 22/393 (5%) Query: 1 MKKSLLALAVLGAFAGAAQAQSSVTIYGIVDTGLVYTSKAVNATTGSTGSKFGLNSGIIQ 60 MKKSLLALAVLGAFAGAAQAQSSVTIYGIVDTG+VY SK V TG TGSKF LNSG+IQ Sbjct: 1 MKKSLLALAVLGAFAGAAQAQSSVTIYGIVDTGVVYQSK-VATPTGGTGSKFSLNSGVIQ 59 Query: 61 GSRIGFKGVEDXGG-LSAVFQLETGFNNDNGALNGQDAGSTNLFRRKSVVGLSGGFGTVL 119 GSRIGFKG ED GG LSAVF LE GF+ND GAL+ +T LFRRKSVVGL+G FG+VL Sbjct: 60 GSRIGFKGTEDLGGGLSAVFNLEAGFSNDTGALDDSKTSNT-LFRRKSVVGLAGNFGSVL 118 Query: 120 AGRQTDFADTISAYTSVNDFGGVTGAVGHNLDRLEGTRTNNSISYTTANLSGFTGNLMYG 179 GRQTDFADTIS+YTSV DFGG TGA GHNLDRLEGTRTNNSISYTTANL+GFTGNLMYG Sbjct: 119 LGRQTDFADTISSYTSVQDFGGWTGAAGHNLDRLEGTRTNNSISYTTANLNGFTGNLMYG 178 Query: 180 FGETAGKTSAGQSYGIGGKYDNGPLGLGINYYQSKQGSTPSDTSLIST------ANGAQA 233 FGETAG+TSAGQ++G+GGKYDNGPLGLGINYYQSK GSTP+D SL++ NG Sbjct: 179 FGETAGQTSAGQAFGVGGKYDNGPLGLGINYYQSKAGSTPADASLLAATGTNQFTNGTNN 238 Query: 234 GNTAFKGLNVVASYQFGPARVYGNWSRVKQDLNTSTTLA------------RSLGNTTKA 281 G+TA K LNV ASYQFGPARVYGNWSRVKQDLNT L R+L + KA Sbjct: 239 GSTAQKVLNVAASYQFGPARVYGNWSRVKQDLNTQANLTAAGVGSLATISNRTLAASNKA 298 Query: 282 DVYELGTAYSLSPSLKLLASVQHTRADFANGGNKGKLTQFNLGTDYWLSKRTDLYAFVSN 341 D+YE+GTAYSLSPSLKLLA+V HTRA F K KLTQ +LGTDYWLSKRTDLYAF++ Sbjct: 299 DIYEIGTAYSLSPSLKLLAAVDHTRAQFDGVSGKAKLTQVSLGTDYWLSKRTDLYAFLAY 358 Query: 342 IRATDMINTGVYGDTTGSDASQTAVAVGVRHKF 374 +ATD+ N GV D++G+D +Q AVAVG+RHKF Sbjct: 359 SKATDLNNPGV-ADSSGTDGNQGAVAVGIRHKF 390 >gi|300310328|ref|YP_003774420.1| porin signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 405 Score = 294 bits (752), Expect = 1e-80, Method: Compositional matrix adjust. Identities = 193/414 (46%), Positives = 244/414 (58%), Gaps = 49/414 (11%) Query: 1 MKKSLLALAVLGAFAGAAQAQSS-VTIYGIVDTGLVYTSKAVNATTG-STGSKFGLNSGI 58 MKKSLLALAVLGAFAGAA AQSS +TIYG +D L YT+K AT G +TGS + SG+ Sbjct: 1 MKKSLLALAVLGAFAGAAHAQSSSITIYGAIDASLTYTNKI--ATDGKNTGSLTAVESGL 58 Query: 59 IQGSRIGFKGVEDXGG-LSAVFQLETGFNNDNGALNGQDAGSTNL-FRRKSVVGLSGGFG 116 ++GSR+G KG ED GG L AVF LE GFN D G +AG L + R++ VGLSG FG Sbjct: 59 VKGSRLGIKGSEDLGGGLKAVFTLENGFNVDTG-----EAGQGKLMWGRQTTVGLSGNFG 113 Query: 117 TVLAGRQTDFADTISAYTSVNDFGGVTGAV-GHNLDRLEGTRTNNSISYTTANLSGFTGN 175 TVL GRQ D + I S F GV G V LDR +G R NNSI Y + N +G + + Sbjct: 114 TVLVGRQNDPLNDIGGIASAGGF-GVPGTVHSKGLDRTDGQRLNNSIRYNSPNFNGLSFS 172 Query: 176 LMYGFGETAGKTSAGQSYGIGGKYDNGPLGLGINYYQSKQG---STPSDTSLISTANGA- 231 +YGFGE G+ G+S G+G +Y NGPL LG+ Y+QSK G SD + + NGA Sbjct: 173 GIYGFGEQVGRAVGGRSIGLGSQYVNGPLSLGLGYFQSKMGGGSDGSSDVNAKAACNGAG 232 Query: 232 ---QAGNTAFKGLNVVASYQFGPARVYGNWSRVKQDL----------------------- 265 AG+T K + A+YQFGPA+VYG +S V+ L Sbjct: 233 EKDHAGDTCLKTWTLAAAYQFGPAKVYGAFSHVRLPLAKLHTKVRKDNNFGAMYDAKTGT 292 Query: 266 --NTSTTLARSLG--NTTKADVYELGTAYSLSPSLKLLASVQHTRADFANGGNKGKLTQF 321 N S A S+G N + +++G +Y L PSL L+++VQHTRA F G G+L Q Sbjct: 293 GYNPSDATAFSIGGANHKSSQTFDIGMSYQLMPSLTLMSAVQHTRAKFV-GAASGRLLQL 351 Query: 322 NLGTDYWLSKRTDLYAFVSNIRATDMINTGVYGD-TTGSDASQTAVAVGVRHKF 374 NLG Y LSKRT YA V N+R++DM N G+ D G D SQTA++ G+ H F Sbjct: 352 NLGAKYDLSKRTSAYALVRNLRSSDMYNVGLSKDQEPGIDRSQTAISTGLIHTF 405 >gi|300312191|ref|YP_003776283.1| outer membrane porin [Herbaspirillum seropedicae SmR1] Length = 372 Score = 268 bits (685), Expect = 6e-73, Method: Compositional matrix adjust. Identities = 165/366 (45%), Positives = 219/366 (59%), Gaps = 34/366 (9%) Query: 24 VTIYGIVDTGLVYTSKAVNATTGSTGSKFGLNSGIIQGSRIGFKGVEDXG-GLSAVFQLE 82 VT+YG++D LVYTSK GS+ L+SG SRIG +G E+ G GL+A+FQLE Sbjct: 26 VTVYGVIDMSLVYTSK----VGPDNGSRASLDSGDAMASRIGLRGREELGDGLAAIFQLE 81 Query: 83 TGFNNDNGALNGQDAGSTNLFRRKSVVGLSGGFGTVLAGRQTDFADTI-SAYTSVNDFGG 141 TGF D+GA+ A LF RKSVVGL+G FGT+ GRQTD+ + I YTS FGG Sbjct: 82 TGFAGDDGAM----ATPGTLFDRKSVVGLAGRFGTLTVGRQTDYLEEIGQQYTSFQIFGG 137 Query: 142 --VTGAVGHNLDRLEGTRTNNSISYTTANLSGFTGNLMYGFGETAGKTSAGQSYGIGGKY 199 V A ++LDR+ G R+NNS+ + TA G G+ YG GE AG T +GQS G+GG + Sbjct: 138 GGVRAAHFNSLDRITGARSNNSLRFDTAEYGGLRGSFYYGAGEVAGSTRSGQSLGVGGNF 197 Query: 200 DNGPLGLGINYYQSKQGSTPSDTSLISTANGAQAGNTAFKGLNVVASYQFGPARVYGNWS 259 G +G+G Y+QSK L S A A+AG+T K + ASYQ GPA+++ WS Sbjct: 198 RRGNVGVGAAYFQSK---------LASDALPARAGDTELKTFTLGASYQAGPAKLFAAWS 248 Query: 260 RVKQDLNTSTTLARS----LGNTTKADVYELGTAYSLSPSLKLLASVQHTRADF---ANG 312 ++++ T LA S + + T+A+V ++G Y+L L LLASV H RAD A G Sbjct: 249 QIRR--PTQQPLASSGLVAITSATRANVLDVGVDYALDSKLHLLASVIHDRADLSRAAGG 306 Query: 313 GNKGKLTQFNLGTDYWLSKRTDLYAFVSNIRATDMINTGV----YGDTTGSDASQTAVAV 368 +G TQ NLG D LSKRTD+YA S +A D++N G+ Y D+SQ + V Sbjct: 307 ATRGSTTQVNLGVDLLLSKRTDVYAMASRQQARDVLNPGIISAAYSSAPADDSSQNVLRV 366 Query: 369 GVRHKF 374 G+RHKF Sbjct: 367 GLRHKF 372 >gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 352 Score = 170 bits (431), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 149/389 (38%), Positives = 198/389 (50%), Gaps = 52/389 (13%) Query: 1 MKKSLLALAVLGAFAGAAQAQSSVTIYGIVDTGLVYTSKAVNATTGSTGSKF-GLNSGII 59 MKK+LL A+L A A AQSSV+IYG++D GL Y S A +G G++ ++SGI Sbjct: 1 MKKTLLVAAMLAGGATTAHAQSSVSIYGLIDLGLTYYSSA--PKSGGGGARLLRMDSGIA 58 Query: 60 QGSRIGFKGVEDXGG-LSAVFQLETGFNNDNGALNGQDAGSTNLFRRKSVVGL-SGGFGT 117 QGSRIGFKG ED GG ++A F LETGF D+G+L GQ LF R+S VGL S +G+ Sbjct: 59 QGSRIGFKGTEDLGGGMNAFFTLETGFGADDGSL-GQGG---LLFGRQSFVGLGSTQWGS 114 Query: 118 VLAGRQTDFADTIS------AYTSVNDFG-GVTGAVGH----NLDRLEGTRTNNSISYTT 166 + GRQ DF I A + FG G+ H N G RTNNSI Y + Sbjct: 115 LSVGRQYDFMANIGGQYAMGALSPAGSFGWGLHADAAHGTALNNHIYAGDRTNNSIKYNS 174 Query: 167 ANLSGFTGNLMYGFGETAGKTSAGQSYGIGGKYDNGPLGLGINYYQSKQGSTPSDTSLIS 226 A GF+ M G GETAG +AG++ G+YDNGP + Y K ST S ++ I Sbjct: 175 AKFGGFSFGAMVGLGETAGNNNAGRTVSATGQYDNGPFSGALAYTDIKD-STGSFSTRIY 233 Query: 227 TANGAQAGNTAFKGLNVVASYQFGPARVYGNWSRVKQDLNTSTTLARSLGNTTKADVYEL 286 A YQ G +G ++VK NT+T + KA YEL Sbjct: 234 GGG---------------ARYQIGAFTPFGVVTQVK---NTNT--------SAKATTYEL 267 Query: 287 GTAYSLSPSLKLLASVQHTRADFANGGNKGKLTQFNLGTDYWLSKRTDLYAFVSNIRATD 346 GT Y+L+P L A+ Q + + G + + DY +SKRTDLY R Sbjct: 268 GTVYALTPLWDLAAAYQFQKRNQGVGNAQALVAT----VDYKISKRTDLYVGAVYDRDKG 323 Query: 347 MINTGVY-GDTTGSDASQTAVAVGVRHKF 374 V+ G + +QTA+ +G+RH+F Sbjct: 324 YNAYPVFGGGIQSATGNQTALRLGMRHRF 352 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.313 0.130 0.368 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,150,840 Number of extensions: 53017 Number of successful extensions: 215 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 137 Number of HSP's successfully gapped: 14 Length of query: 374 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 287 Effective length of database: 1,207,925 Effective search space: 346674475 Effective search space used: 346674475 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 131 (55.1 bits)