BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0025.p (533 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313594|ref|YP_003777686.1| alkyl hydroperoxide reductase s... 1064 0.0 gi|300311227|ref|YP_003775319.1| thioredoxin reductase oxidoredu... 150 2e-37 gi|300310953|ref|YP_003775045.1| thioredoxin reductase oxidoredu... 66 8e-12 >gi|300313594|ref|YP_003777686.1| alkyl hydroperoxide reductase subunit F [Herbaspirillum seropedicae SmR1] Length = 533 Score = 1064 bits (2752), Expect = 0.0, Method: Compositional matrix adjust. Identities = 526/533 (98%), Positives = 529/533 (99%) Query: 1 MLDANLKTQLKAYLEKVSHPIEIVASLDDGAKSREMQELLQDIVLLSDRIKLVEQRGADA 60 MLDANLKTQLKAYLEKVSHPIEIVASLDDGAKSREMQELLQDIVLLSDRIKLVEQRGADA Sbjct: 1 MLDANLKTQLKAYLEKVSHPIEIVASLDDGAKSREMQELLQDIVLLSDRIKLVEQRGADA 60 Query: 61 QGRTPSFRLNRPGTPISVEFAAIPMGHEFTSLVLALLQVGGHPVKLDDKVIEQIKNLDGD 120 QGRTPSF+LNRPGT ISVEFAAIPMGHEFTSLVLALLQVGGHPVKLDDKVIEQIKNLDGD Sbjct: 61 QGRTPSFKLNRPGTDISVEFAAIPMGHEFTSLVLALLQVGGHPVKLDDKVIEQIKNLDGD 120 Query: 121 YRFETYVSLTCQNCPEVVQALNVMSIINPRIQSVTIDGALFQGEVEQRQIMAVPTVYMNG 180 YRFETYVSLTCQNCPEVVQALNVMSIINPRIQSVTIDGALFQGEVEQRQIMAVPTVYMNG Sbjct: 121 YRFETYVSLTCQNCPEVVQALNVMSIINPRIQSVTIDGALFQGEVEQRQIMAVPTVYMNG 180 Query: 181 EVFGQGRTGVEEILAKLDTGAAARKGEELSAKEPYDVLIVGGGPAGAAAAIYAARKGIRT 240 EVFGQGRTGVEEILAKLDTGAAARKGEELSAKEPYDVLIVGGGPAGAAAAIYAARKGIRT Sbjct: 181 EVFGQGRTGVEEILAKLDTGAAARKGEELSAKEPYDVLIVGGGPAGAAAAIYAARKGIRT 240 Query: 241 GVVAERFGGQVLDTLAIENFISVQETEGPQFATALEQHVRAYDVDIMNVQRAEALVPGDK 300 GVVAERFGGQVLDTLAIENFISVQETEGPQFATALEQHV+AYDVDIMNVQRAEALVPGDK Sbjct: 241 GVVAERFGGQVLDTLAIENFISVQETEGPQFATALEQHVKAYDVDIMNVQRAEALVPGDK 300 Query: 301 LIEVKLANGGVLKGKTVILSTGARWRNVNVPGEQEYKNKGVAYCPHCDGPLFKGKRVSVI 360 LIEVKLANGGVLKGKTVILSTGARWRNVNVPGEQEYKNKGVAYCPHCDGPLFKGKRVSVI Sbjct: 301 LIEVKLANGGVLKGKTVILSTGARWRNVNVPGEQEYKNKGVAYCPHCDGPLFKGKRVSVI 360 Query: 361 GGGNSGVEAAIDLAGIVAHVTLIEFAAELKADAVLVRKLKSLPNVTIVTSAQTTEITGDG 420 GGGNSGVEAAIDLAGIVAHVTLIEFAAELKADAVLVRKLKSLPNVTIVTSAQTTEITGDG Sbjct: 361 GGGNSGVEAAIDLAGIVAHVTLIEFAAELKADAVLVRKLKSLPNVTIVTSAQTTEITGDG 420 Query: 421 QRVNGISYKERETGEVKHVELEGVFVQIGLVPNTEWLKGTLALSKHGEIEIDAKGQTSIP 480 QRVNGISYKERETGE+KHV LEGVFVQIGLVPNTEWLKGTLALSKHGEIEIDAKGQTSIP Sbjct: 421 QRVNGISYKERETGEIKHVPLEGVFVQIGLVPNTEWLKGTLALSKHGEIEIDAKGQTSIP 480 Query: 481 GVFAAGDVTTVPYKQIVIAVGAGATAGLSAFDHLIRSSADDVAPAVAKEAVAA 533 GVFAAGDVTTVPYKQIVIAVGAGATA LSAFDHLIRSSADDVAPA AKEAVAA Sbjct: 481 GVFAAGDVTTVPYKQIVIAVGAGATAALSAFDHLIRSSADDVAPAAAKEAVAA 533 >gi|300311227|ref|YP_003775319.1| thioredoxin reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 318 Score = 150 bits (379), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 105/317 (33%), Positives = 165/317 (52%), Gaps = 31/317 (9%) Query: 217 VLIVGGGPAGAAAAIYAARKGIR----TGVVAERFGGQVLDTLAIENF----ISVQETEG 268 VLI+G GPAG +AA+YAAR + TGV GGQ++ T +EN+ + VQ G Sbjct: 10 VLILGSGPAGYSAAVYAARANLNPVLITGV---EQGGQLMTTTDVENWPGDPLGVQ---G 63 Query: 269 PQFATALEQHVRAYDVDIMNVQRAEALVPGDKLIEVK---LANGGVLKGKTVILSTGARW 325 P+ L QH + +I+ + KL E + + G +I++TGA Sbjct: 64 PELMQRLLQHAERFKTEIIFDH-----IHTTKLSERPFRLIGDSGEYTADALIIATGASA 118 Query: 326 RNVNVPGEQEYKNKGVAYCPHCDGPLFKGKRVSVIGGGNSGVEAAIDLAGIVAHVTLIEF 385 + + +P EQ + +GV+ C CDG ++GK V+V+GGGN+ VE A+ L+ I + VT+I Sbjct: 119 QYLGLPSEQAFMGRGVSACATCDGFFYRGKEVAVVGGGNTAVEEALYLSNIASKVTIIHR 178 Query: 386 AAELKADAVLVRKLK---SLPNVTIVTSAQTTEITGDGQRVNGISYKERETGEVKHVELE 442 + +A+ +L+ +L S + I E+ GD V GI K + + + + Sbjct: 179 RDKFRAEPILIDRLLHKVSEGKIAIQWHHTLDEVVGDDSGVTGIKIKSTQDDAITEIPVH 238 Query: 443 GVFVQIGLVPNTEWLKGTLALSKHGEIEIDAKGQ-----TSIPGVFAAGDVTTVPYKQIV 497 G+F+ IG PNT +G L++ ++G ++ + TSI GVFAAGDV Y+Q + Sbjct: 239 GLFIAIGHKPNTSIFEGQLSM-QNGYLKTRTGTEGFATATSIDGVFAAGDVQDHVYRQAI 297 Query: 498 IAVGAGATAGLSAFDHL 514 + G G A L A +L Sbjct: 298 TSAGTGCMAALDAQRYL 314 >gi|300310953|ref|YP_003775045.1| thioredoxin reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 357 Score = 65.9 bits (159), Expect = 8e-12, Method: Compositional matrix adjust. Identities = 76/315 (24%), Positives = 132/315 (41%), Gaps = 47/315 (14%) Query: 216 DVLIVGGGPAGAAAAIYAARKGIRTGVVAER--FGGQVLDTLA---IENFISVQETEGPQ 270 D +I+G GP G I+ V+ GGQ ++ I + +V G + Sbjct: 17 DAVIIGAGPVGLFQVFELGLLEIKAHVIDSLPVVGGQCVELYPDKPIYDIPAVPVCTGQE 76 Query: 271 FATALEQHVRAY--------DVDIMNVQRAEALVPGDKLIEVKLANGGVLKGKTVILSTG 322 L + + + +V ++N + D +V+ + G KT+ ++ G Sbjct: 77 LTDNLLKQIEPFSPTFHLGQEVTLVNKRE-------DGRFDVETSTGTKFITKTIFIAAG 129 Query: 323 A---RWRNVNVPGEQEYKNKGVAYCPHCDGPLFKGKRVSVIGGGNSGVEAAIDLAGIVAH 379 + R + V G ++++ V Y D LF+GK + + GGG+S ++ A++ G Sbjct: 130 VGSFQPRTLKVEGIEQFEGSQVFYRVK-DPALFEGKNIVICGGGDSALDWALNFVGKAES 188 Query: 380 VTLIEFAAELKADAVLVRKLKSLPNVTIVTSAQTTEITGDGQRVNGISYKERETGEVK-- 437 V L+ E +A V K+K L + E+ +V G K+ + EVK Sbjct: 189 VILLHRREEFRAAPASVAKMKEL--------CEQYEMQLLIGQVTGTEIKDGKLAEVKVT 240 Query: 438 -------HVELEGVFVQIGLVPN----TEWLKGTLALSKHGEIEIDAKGQTSIPGVFAAG 486 + L+ + V GL P EW G K ++ K +T++PG+FA G Sbjct: 241 GADGVTRRLPLDMLLVFFGLSPKLGPIAEW--GLEIERKQLKVMDTEKFETNVPGIFAVG 298 Query: 487 DVTTVPYKQIVIAVG 501 D+ T P K+ +I G Sbjct: 299 DINTYPGKKKLILSG 313 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.135 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,481,622 Number of extensions: 60482 Number of successful extensions: 253 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 250 Number of HSP's successfully gapped: 4 Length of query: 533 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 443 Effective length of database: 1,193,720 Effective search space: 528817960 Effective search space used: 528817960 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)