BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0207.p (426 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313197|ref|YP_003777289.1| threonine dehydratase [Herbaspi... 860 0.0 gi|300309398|ref|YP_003773490.1| threonine dehydratase [Herbaspi... 110 2e-25 gi|300309629|ref|YP_003773721.1| threonine dehydratase [Herbaspi... 110 2e-25 gi|300311135|ref|YP_003775227.1| threonine dehydratase [Herbaspi... 70 2e-13 >gi|300313197|ref|YP_003777289.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 508 Score = 860 bits (2221), Expect = 0.0, Method: Compositional matrix adjust. Identities = 419/426 (98%), Positives = 424/426 (99%) Query: 1 VALSAARLGCRAVIVMPTTTPQVKIDAVKARGGEVVLFGDSFTDAYEHALTLEKKQKLTF 60 VALSAARLGCRAVIVMPTTTPQVKIDAVKARGGEVVLFGDSFTDAYEHALTLEKKQKLTF Sbjct: 83 VALSAARLGCRAVIVMPTTTPQVKIDAVKARGGEVVLFGDSFTDAYEHALTLEKKQKLTF 142 Query: 61 VHPFDDPYVIAGQGTVGMEILRQHPEPIHAIFVAIGGGGLIAGVASYVKAVRPDIKIIGV 120 VHPFDDPYVIAGQGTVGMEILRQHP+PIHAIFVAIGGGGLIAGVASYVKAVRPDIKIIGV Sbjct: 143 VHPFDDPYVIAGQGTVGMEILRQHPDPIHAIFVAIGGGGLIAGVASYVKAVRPDIKIIGV 202 Query: 121 QTTDSDAMARSLKAGRRVALPDVGLFSDGTAVKLVGEETFRIAKELVDEVIIVDTDAVCT 180 QTTDSDAMARSLKAGRRVALPDVGLFSDGTAVKLVGEETFRIAKELVDEVIIVDTDAVCT Sbjct: 203 QTTDSDAMARSLKAGRRVALPDVGLFSDGTAVKLVGEETFRIAKELVDEVIIVDTDAVCT 262 Query: 181 AIKDIFQDTRSIVEPAGALAVAGAKAYVERAKASKKPIKGESLVTIACGANMNFDRLRFV 240 AIKDIFQDTRSIVEPAGALAVAGAKAYVERAKASKKPIKGESL+TIACGANMNFDRLRFV Sbjct: 263 AIKDIFQDTRSIVEPAGALAVAGAKAYVERAKASKKPIKGESLITIACGANMNFDRLRFV 322 Query: 241 AEMADNGEAREAVFAVTIPEERGSFRRFCETVGPRNVTEFNYRISDAKAAHVFVGIQVSA 300 AEMAD GEAREAVFAVTIPEERGSFRRFCETVGPRNVTEFNYRISDAKAAHVFVGIQVSA Sbjct: 323 AEMADAGEAREAVFAVTIPEERGSFRRFCETVGPRNVTEFNYRISDAKAAHVFVGIQVSA 382 Query: 301 ADEAGKIARNFEKAGFGVLDLTHDELAKVHIRHLVGGKSELAGDELLYRFEFPERPGALM 360 ADEAGKIARNFEKAGFGVLDLTHDELAKVHIRHLVGGKS+L GDELLYRFEFPERPGALM Sbjct: 383 ADEAGKIARNFEKAGFGVLDLTHDELAKVHIRHLVGGKSDLTGDELLYRFEFPERPGALM 442 Query: 361 KFLSSMNPGWNISLFHYRNQGGDVGRILIGVQVPKKEMKEFRAFLAQLGYRHWDETKNPL 420 KFLSSMNPGWNISLFHYRNQGGDVGRILIG+QVPKKEMKEFRAFLAQLGYRHWDE+KNPL Sbjct: 443 KFLSSMNPGWNISLFHYRNQGGDVGRILIGLQVPKKEMKEFRAFLAQLGYRHWDESKNPL 502 Query: 421 YKLFLG 426 YKLFLG Sbjct: 503 YKLFLG 508 >gi|300309398|ref|YP_003773490.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 401 Score = 110 bits (276), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 77/194 (39%), Positives = 114/194 (58%), Gaps = 3/194 (1%) Query: 1 VALSAARLGCRAVIVMPTTTPQVKIDAVKARGGEVVLFGDSFTDAYEHALTLEKKQKLTF 60 VA A RLG A IVMP TP VKI+ + G VVL GD+ A HA L +K+ LTF Sbjct: 84 VAYHAQRLGLPATIVMPRFTPGVKIERTRGFGANVVLHGDTLEAARTHAYELAEKEGLTF 143 Query: 61 VHPFDDPYVIAGQGTVGMEILRQHPEPIHAIFVAIGGGGLIAGVASYVKAVRPDIKIIGV 120 VHP+DD +IAGQGTV +E+++ P+ + + V+IGGGGL+AG+A +A++P+I+I+GV Sbjct: 144 VHPYDDEAIIAGQGTVALEMMKAVPD-LDCLVVSIGGGGLLAGMAVAARAIKPEIEIVGV 202 Query: 121 QTTDSDAMARSLKAGRRVALPDVGLFSDGTAVKLVGEETFRIAKELVDEVIIVDTDAVCT 180 QT M ++K ++G AV G T I DE+++VD + Sbjct: 203 QTRRFPTMVNAVKGTEHPV--GASTIAEGIAVGTPGTLTRAIIAREADELLLVDEGDIEQ 260 Query: 181 AIKDIFQDTRSIVE 194 A+ + + +++VE Sbjct: 261 AVLMLLEIEKTLVE 274 >gi|300309629|ref|YP_003773721.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 326 Score = 110 bits (276), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 81/238 (34%), Positives = 132/238 (55%), Gaps = 10/238 (4%) Query: 1 VALSAARLGCRAVIVMPTTTPQVKIDAVKARGGEVVLFGDSFTDAYEH-ALTLEKKQKLT 59 AL+A LG AVIVMP P K+ A + G EVV + D +T+ E L ++K+T Sbjct: 92 TALAAQLLGIPAVIVMPQDAPAAKVAATRGYGAEVVTY-DRYTEDREAIGARLAAERKMT 150 Query: 60 FVHPFDDPYVIAGQGTVGMEILRQHPEPIHAIFVAIGGGGLIAGVASYVKAVRPDIKIIG 119 + P+D P+V+AGQGT +E+L Q P+ A+FV +GGGGL++G A +A+ P KI G Sbjct: 151 LIPPYDHPHVMAGQGTATLELL-QETGPLDALFVCLGGGGLLSGAALAARALSPACKIYG 209 Query: 120 VQTTDSDAMARSLKAGRRVALPDVGLFSDGTAVKLVGEETFRIAKELVDEVIIVDTDAVC 179 V+ + +SL+AG+ V + +DG + +G+ TF + +ELVD++ V + + Sbjct: 210 VEPQAGNDGQQSLRAGQVVHIDTPKTIADGAQTQHLGKYTFGVIRELVDDIHTVSDEELV 269 Query: 180 TAIKDIFQDTRSIVEPAGALAVAGAKAYVERAKASKKPIKGESLVTIACGANMNFDRL 237 ++ + + +VEP G L A A+ K ++G+ + I G N++ +L Sbjct: 270 AGMRFFAERMKMVVEPTGCLGFAAARKL-------KDQLQGKRVGVIISGGNVDLGKL 320 >gi|300311135|ref|YP_003775227.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 351 Score = 70.5 bits (171), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 57/192 (29%), Positives = 96/192 (50%), Gaps = 2/192 (1%) Query: 1 VALSAARLGCRAVIVMPTTTPQVKIDAVKARGGEVVLFGDSFTDAYEHALTLEKKQKLTF 60 V L+A RLG A IV+P + K A++A G E++ G F ++ E+A L +Q+L Sbjct: 92 VGLAARRLGISATIVVPQGNSREKNAAMRALGVELIEHGSEFQESREYAQQLAAQQQLHM 151 Query: 61 VHPFDDPYVIAGQGTVGMEILRQHPEPIHAIFVAIGGGGLIAGVASYVKAVRPDIKIIGV 120 + P ++AG + ME+ P + + V IG G + G + A+ +IIGV Sbjct: 152 I-PSLHRDLVAGVASYWMELFAAQPG-LDVVLVPIGQGSGMCGAVAARNALGLSTRIIGV 209 Query: 121 QTTDSDAMARSLKAGRRVALPDVGLFSDGTAVKLVGEETFRIAKELVDEVIIVDTDAVCT 180 + + A S +AGR++ P +DG A ++ + + + VDEV+ V D V Sbjct: 210 VSAHALAYKLSFEAGRKIESPVSTCIADGVACRVPDQASLEVLFSEVDEVVAVTDDEVMD 269 Query: 181 AIKDIFQDTRSI 192 A+K + T ++ Sbjct: 270 AMKLAYIATHNV 281 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.138 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,213,824 Number of extensions: 48218 Number of successful extensions: 151 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 146 Number of HSP's successfully gapped: 4 Length of query: 426 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 338 Effective length of database: 1,203,190 Effective search space: 406678220 Effective search space used: 406678220 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)