BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0186.p (294 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313216|ref|YP_003777308.1| LysR family transcription regul... 484 e-138 gi|300311633|ref|YP_003775725.1| LysR family transcription regul... 115 4e-27 gi|300309864|ref|YP_003773956.1| LysR family transcription regul... 66 4e-12 gi|300313217|ref|YP_003777309.1| LysR family transcription regul... 65 5e-12 gi|300309386|ref|YP_003773478.1| LysR family transcription regul... 56 4e-09 >gi|300313216|ref|YP_003777308.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 303 Score = 484 bits (1245), Expect = e-138, Method: Compositional matrix adjust. Identities = 262/282 (92%), Positives = 274/282 (97%) Query: 1 VARASERLHLTAQTISGQISLFEESLGYKLFKRVGRRLELNDEGRMVFDYAERIFSLGEE 60 VARASERLHLTAQTISGQ+SLFEESLGY LF+RVGRRLELNDEGRMVFDYAERIFS+GEE Sbjct: 21 VARASERLHLTAQTISGQLSLFEESLGYPLFRRVGRRLELNDEGRMVFDYAERIFSIGEE 80 Query: 61 LEEALRLRPGGRTLQLRVGVADAVPKAIAYLLLESALAMQDPIRIICREGKLDVLLADLA 120 LEEALRLRPGGR LQLRVGVADAVPKAIAYLLLESALAMQDPIRIICREGKLDVLLADLA Sbjct: 81 LEEALRLRPGGRALQLRVGVADAVPKAIAYLLLESALAMQDPIRIICREGKLDVLLADLA 140 Query: 121 IHKLDIIIADSPMPPNVDVRGYHHLLGECDTSFFATPELARRYRKGFPQSLDAAPFLMPG 180 +HKLDIIIADSPMPPNVDVRGYHHLLGECDTSFFATPELARRYRKGFP S D APFLMPG Sbjct: 141 LHKLDIIIADSPMPPNVDVRGYHHLLGECDTSFFATPELARRYRKGFPGSFDGAPFLMPG 200 Query: 181 EDAAVRPRLLRWFEKEGIRPQITGEFDDGALLLAFGDAGAGIFAAPTAIAGQIRKQYGLV 240 EDAAVRPRLLRWFEKE IRP+ITGEFDDGALLLAFGDAGAGIFAAP AIA QIRKQYGLV Sbjct: 201 EDAAVRPRLLRWFEKEKIRPRITGEFDDGALLLAFGDAGAGIFAAPAAIAAQIRKQYGLV 260 Query: 241 EIGKTDAIREQFYAISVERRLSHPAVLAIQTAARGNLIVGST 282 EIG+T+AIREQFYAISV+R+L+HPAVLAIQ+AARGNLIVGS+ Sbjct: 261 EIGRTEAIREQFYAISVQRKLTHPAVLAIQSAARGNLIVGSS 302 >gi|300311633|ref|YP_003775725.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 306 Score = 115 bits (288), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 89/284 (31%), Positives = 134/284 (47%), Gaps = 19/284 (6%) Query: 1 VARASERLHLTAQTISGQISLFEESLGYKLFKRVGRRLELNDEGRMVFDYAERIFSLGEE 60 + RA+E LH + +S QI E+ LG LF R GRRL L+D G++VF YA+ IF L +E Sbjct: 23 LTRAAEELHTSQSAVSAQIRQLEKHLGEDLFIREGRRLSLSDTGQLVFTYADNIFGLSQE 82 Query: 61 LEEALRLRPGGRTLQLRVGVADAVPKAIAYLLLESALAMQDP-IRIICREGKLDVLLADL 119 + L R G T +LRVG + + L L+ DP + + G+L+ LL L Sbjct: 83 MLGRLEGRSAGVT-RLRVGSVATLSRNYQENWLRPMLS--DPSVALTLESGQLEGLLERL 139 Query: 120 AIHKLDIIIADSPMPPNVDVRGYHHLLGECDTSFFATPELARRYRKG---FPQSLDAAPF 176 H+LD+++A+ +P + D + LG S A R+ G P+ L+ Sbjct: 140 VQHQLDVVLANETVPADPDRPLHCRFLGSQAVSVVGP---AHRWEAGSLRIPEDLEGIDM 196 Query: 177 LMPGEDAAVRPRLLRWFEKEGIRPQITGEFDDGALLLAFGDAGAGIFAAPTAIAGQIRKQ 236 +PG A+R R +RP++ E DD A+L + P + + Sbjct: 197 ALPGPRHALRMRFDALCVSADVRPRLRAEVDDMAMLRLIARDSGWLSVLPEVVVQDELRN 256 Query: 237 YGLVEIGKTDAIREQFYAIS---------VERRLSHPAVLAIQT 271 LV +G+T + E FYAI+ VER L+ A A +T Sbjct: 257 GVLVTVGQTTELVENFYAITTPQHHYGGLVERLLNGSAATARET 300 >gi|300309864|ref|YP_003773956.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 306 Score = 65.9 bits (159), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 70/251 (27%), Positives = 113/251 (45%), Gaps = 25/251 (9%) Query: 3 RASERLHLTAQTISGQISLFEESLGYKLFKRVGRRLELNDEGRMVFDYAERIFSLGEELE 62 RA+ L++T +S QI L E LG +LF+RVGR + L EGR+ A R+ L E+ E Sbjct: 21 RAAALLNMTQPPLSRQIHLLEHRLGVQLFERVGRTVRLTTEGRVFLADASRLLQLSEQAE 80 Query: 63 EALRLRPGGRTLQLRVGVADAV-PKAIAYLLLESALAMQDPIRIICREGKLDVLLADL-A 120 +R G+T ++RVG A + I LL+++ ++ + +DV+L +L + Sbjct: 81 STVRRASQGKTGRVRVGFTGAAGYELIPELLMQARTSLPE----------IDVVLFELIS 130 Query: 121 IHKLDIIIADSP----MPPNVDVRGYHHLLGECDTSFFATPE---LARRYRKGFPQSLDA 173 + +L+ A++ + P + L + + A P+ LA R Q LD Sbjct: 131 VAQLEAFTANTIDIGFLRPVASRQPLELQLVDEEALVLAMPQGHALAAYDRIATTQ-LDG 189 Query: 174 APFLM--PGEDAAVRPRLLRWFEKEGIRPQITGEFDDGALLLAFGDAGAGIFAAPTAIAG 231 PF+M P R++ ++P T D +L+ AG G+ P A Sbjct: 190 QPFIMHSPTHGKYFYERIMAMLGAADVKPDFTQYIDQSHTILSLVRAGLGVGILP---AS 246 Query: 232 QIRKQYGLVEI 242 R Y VE Sbjct: 247 AQRFHYDRVEF 257 >gi|300313217|ref|YP_003777309.1| LysR family transcription regulator [Herbaspirillum seropedicae SmR1] Length = 296 Score = 65.5 bits (158), Expect = 5e-12, Method: Compositional matrix adjust. Identities = 70/255 (27%), Positives = 105/255 (41%), Gaps = 38/255 (14%) Query: 1 VARASERLHLTAQTISGQISLFEESLGYKLFKRVGRRLELNDEGRMVFDYAERIFSLGEE 60 V RA+ERL+ ++ +I E+S+G LF R RR+ L G + DYAERI L +E Sbjct: 27 VTRAAERLNRVQSNVTTRIKQLEQSMGVPLFVRDRRRMVLTAAGEALLDYAERILDLAQE 86 Query: 61 LEEALRLR-PGGRTLQLRVGVADAVPKAIAYLLLESALAMQDP------------IRIIC 107 +A+ + P GR LR+G +ESA A + P +R+ Sbjct: 87 ARQAVAPQSPRGR---LRIGS------------MESAAASRLPGPLAEFHQRWPEVRLEL 131 Query: 108 REGKLDVLLADLAIHKLDIIIADSPMP-PNVDVRGYHHLLGECDTSFFATPELARRYRKG 166 DVL+A + KLD + P+ P D + TP RR R Sbjct: 132 STAPTDVLMAQVRACKLDAALVAGPVSDPAFDATPLFR-----EELLLVTPRSHRRVRA- 185 Query: 167 FPQSLDAAPFLMPGEDAAVRPRLLRWFEKEGIRPQI--TGEFDDGALLLAFGDAGAGIFA 224 P+ + ++ + A R WF G Q+ T E +LA AGAG+ Sbjct: 186 -PEEVMLDTLIVFEQGCAYRRHAEAWFAGHGAGRQLPRTLELASYHAILACVSAGAGVAV 244 Query: 225 APTAIAGQIRKQYGL 239 P ++ + +G Sbjct: 245 VPRSVIAMQMEPHGF 259 >gi|300309386|ref|YP_003773478.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 301 Score = 55.8 bits (133), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 51/229 (22%), Positives = 99/229 (43%), Gaps = 10/229 (4%) Query: 2 ARASERLHLTAQTISGQISLFEESLGYKLFKRVGRRLELNDEGRMVFDYAERIFSLGEEL 61 +RA++ LH+T T+S + EE + L R GRRL+L D GR+V++ ++I + + L Sbjct: 19 SRAADALHVTQSTVSKMVRQLEEEVDAPLLIRDGRRLQLTDTGRVVYEQGQQILAAMQRL 78 Query: 62 EEALRLRPGGRTLQLRVGVADAVPKAIAYLLLESALAMQDPIRIICREGKLDVLLADLAI 121 +R R+ +L +G+ + + +L++ I ++ +E + +A Sbjct: 79 HAEVRDTKALRSGRLEIGIPPMI-NILCTPVLKAFRERHPDIAVVLQEDTGPQIEQRVAN 137 Query: 122 HKLDIIIADSPMPPNVDVR----GYHHLLGECDTSFFATPELARRYRKGFPQSLDAAPFL 177 +L+I + P P + + + + F + R + LD P + Sbjct: 138 GELEIGMTVLPADPELGLETLPVASYPMWAVAQAGSFQKNRASLRCAQ-----LDGMPLV 192 Query: 178 MPGEDAAVRPRLLRWFEKEGIRPQITGEFDDGALLLAFGDAGAGIFAAP 226 + + + L F + GI+P+I + L+A AG G P Sbjct: 193 LLNNEFGLTRSLRGVFREHGIKPEIVAQSAQWDWLVAMALAGMGTALLP 241 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.140 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 808,731 Number of extensions: 30265 Number of successful extensions: 151 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 151 Number of HSP's successfully gapped: 7 Length of query: 294 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 210 Effective length of database: 1,222,130 Effective search space: 256647300 Effective search space used: 256647300 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)