BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0176.p (789 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313226|ref|YP_003777318.1| protein exporter of the Resista... 1498 0.0 gi|300312823|ref|YP_003776915.1| exporter of the RND superfamily... 1333 0.0 gi|300310853|ref|YP_003774945.1| cation/multidrug efflux pump pr... 65 2e-11 >gi|300313226|ref|YP_003777318.1| protein exporter of the Resistance-Nodulation-Cell Division (RND) superfamily protein [Herbaspirillum seropedicae SmR1] Length = 755 Score = 1498 bits (3877), Expect = 0.0, Method: Compositional matrix adjust. Identities = 725/755 (96%), Positives = 747/755 (98%) Query: 35 MAVFSLQLRMDAGFEKQIPSDHEYVATLHQYRDELFGANRLNIVLRARHGSIWTRDGLTR 94 MAVFSLQLRMDAGFEKQIPSDHEYVAT HQYRDELFGANRLNIVLRARHGSIWT++GLTR Sbjct: 1 MAVFSLQLRMDAGFEKQIPSDHEYVATQHQYRDELFGANRLNIVLRARHGSIWTKEGLTR 60 Query: 95 LNAMTQAVMFLPNINRLGVQSLWTPNTFVNEITEEGFRADPVIAGTVTPDNLTPEVIAGI 154 LNAMTQAVMFLPN+NRLGVQSLWTPNTFVNEITEEGFRADPVIAGTVTP++LTPEVIA I Sbjct: 61 LNAMTQAVMFLPNVNRLGVQSLWTPNTFVNEITEEGFRADPVIAGTVTPESLTPEVIASI 120 Query: 155 RDSANNGGFVGTLVSRSQDSAMIIAEINEFDANGKKVDYLAYNKLLNDLRAKYENADFEV 214 RDSANNGGFVGTLVSR QDSAMIIAEINEFDANGKKVDYLAYNKLLNDLRAKYENADFEV Sbjct: 121 RDSANNGGFVGTLVSRKQDSAMIIAEINEFDANGKKVDYLAYNKLLNDLRAKYENADFEV 180 Query: 215 QIIGFAKQIGDVADGAQGVLKFCVMALLLTALAVYWYCRSLPFTLLPIVCSLVSLVWQFG 274 QIIGFAKQIGDVADGAQGVLKFC+MALLLTALAVYWYCRSLPFTLLPIVCSLVSLVWQFG Sbjct: 181 QIIGFAKQIGDVADGAQGVLKFCIMALLLTALAVYWYCRSLPFTLLPIVCSLVSLVWQFG 240 Query: 275 TLHLLGYGLDPLAVLVPFLVFAIGVSHGVQQINYIVRGIAAGMGCDEAARASFRGLLVPG 334 TLHLLGYGLDPLAVLVPFLVFAIGVSHGVQQINYIVRGIAAGMGC+EAARASFRGLLVPG Sbjct: 241 TLHLLGYGLDPLAVLVPFLVFAIGVSHGVQQINYIVRGIAAGMGCEEAARASFRGLLVPG 300 Query: 335 VLALVTAFVSFITLTLIPIPMVKELAITASIGVGYKIITNLVMLPLAASLLKVDKAYADA 394 VLALVTAFVSFITLTLIPIPMVKELAITASIGVGYKIITNLVMLPLAASLLKV+KAYADA Sbjct: 301 VLALVTAFVSFITLTLIPIPMVKELAITASIGVGYKIITNLVMLPLAASLLKVNKAYADA 360 Query: 395 AMRAQQKRARWLQVFARAAHPRNARGIVLVTLLLFAASVWYSSDRLVGTLQAGAPELRAD 454 AMRAQQKRARWLQVFARAAHPRNAR IV+VTL LFAASVWYSSDRLVGTLQAGAPELRAD Sbjct: 361 AMRAQQKRARWLQVFARAAHPRNARVIVVVTLALFAASVWYSSDRLVGTLQAGAPELRAD 420 Query: 455 SRYNLDAKAIAENYDTGLDWLSVILATPENSCDQVAFGRYQDQFDAAMAQVPGVMSVSSF 514 SRYNLDAK+I++NYDTGLDWLSVILATPENSCD+VAFGRYQ+QFDAAM VPGVMSVSSF Sbjct: 421 SRYNLDAKSISDNYDTGLDWLSVILATPENSCDKVAFGRYQEQFDAAMRHVPGVMSVSSF 480 Query: 515 AQQMKIYNEGYNEGNPAMYSVPLDPANYAALAVEIGRVRGFLSKDCSMLATHLYLTDHKA 574 A+QMKIYNEGYNEGNP+MYSVPLDPANYAALAVEIGRVRGFLSKDCSMLATHLYLTDHKA Sbjct: 481 AEQMKIYNEGYNEGNPSMYSVPLDPANYAALAVEIGRVRGFLSKDCSMLATHLYLTDHKA 540 Query: 575 STINGVIAAVKQYRSAHPLPGLSIRLAAGNAGVIAAVNEEVEKSELPMMLYVYAAIVVLV 634 STING+IAAVKQYR+AHPLPGL+IRLAAGNAGVIAAVNEEVEKSELPMMLYVYAAI+VLV Sbjct: 541 STINGIIAAVKQYRAAHPLPGLTIRLAAGNAGVIAAVNEEVEKSELPMMLYVYAAIIVLV 600 Query: 635 FAVYRDLRAVVACCLPLTVATFIGYAFMKQLQIGLTVATLPVMVLAVGIGVDYAFYIYSR 694 FAVYRDLRAVVACCLPLTVATFIGYAFMKQLQIGLTVATLPVMVLAVGIGVDYAFYIYSR Sbjct: 601 FAVYRDLRAVVACCLPLTVATFIGYAFMKQLQIGLTVATLPVMVLAVGIGVDYAFYIYSR 660 Query: 695 LQMHLAAGEAIDVALEKSIQEIGVATIFTAITLAIGVATWSFSQLKFQADMGKLLAFMFI 754 LQMHLAAGEAI VALEKSIQEIGVATIFTAITLAIGVATWSFSQLKFQADMGKLLAFMFI Sbjct: 661 LQMHLAAGEAIAVALEKSIQEIGVATIFTAITLAIGVATWSFSQLKFQADMGKLLAFMFI 720 Query: 755 VNLIMAMTALPAFAVVLERLFPRKGKIKVNSMMSH 789 VNLIMAMTALPAFAVVLER+FPRKGKIKVNSMM+H Sbjct: 721 VNLIMAMTALPAFAVVLERMFPRKGKIKVNSMMNH 755 >gi|300312823|ref|YP_003776915.1| exporter of the RND superfamily protein [Herbaspirillum seropedicae SmR1] Length = 789 Score = 1333 bits (3450), Expect = 0.0, Method: Compositional matrix adjust. Identities = 639/783 (81%), Positives = 706/783 (90%) Query: 7 FLSLARLEALAFRARLPILLALLGFTALMAVFSLQLRMDAGFEKQIPSDHEYVATLHQYR 66 SL RLEA FR R+PIL LL FTA+++ F+LQLRMDAGFEKQ+P+ HEY+ T QYR Sbjct: 7 LFSLDRLEASLFRLRIPILALLLAFTAVLSYFALQLRMDAGFEKQMPTHHEYIDTFKQYR 66 Query: 67 DELFGANRLNIVLRARHGSIWTRDGLTRLNAMTQAVMFLPNINRLGVQSLWTPNTFVNEI 126 D++ GANRLNIVLRARHGSIWT++GLTRL+ MTQAVMFLPNI+RLGVQSLWTPNTFVNEI Sbjct: 67 DDVLGANRLNIVLRARHGSIWTKEGLTRLSEMTQAVMFLPNISRLGVQSLWTPNTFVNEI 126 Query: 127 TEEGFRADPVIAGTVTPDNLTPEVIAGIRDSANNGGFVGTLVSRSQDSAMIIAEINEFDA 186 TEEGFRADPVI GTV P LTPE+I IR++A NGGFVGTL+SR+QDSAMI+AEINEFDA Sbjct: 127 TEEGFRADPVIPGTVIPSALTPEIIEQIRNTAINGGFVGTLISRNQDSAMIVAEINEFDA 186 Query: 187 NGKKVDYLAYNKLLNDLRAKYENADFEVQIIGFAKQIGDVADGAQGVLKFCVMALLLTAL 246 G+KVDYLAYNKLLNDLRAKYENADFEVQIIGFAKQIGDVADGA+GV+KFC+MALLLTA+ Sbjct: 187 KGEKVDYLAYNKLLNDLRAKYENADFEVQIIGFAKQIGDVADGAEGVVKFCIMALLLTAI 246 Query: 247 AVYWYCRSLPFTLLPIVCSLVSLVWQFGTLHLLGYGLDPLAVLVPFLVFAIGVSHGVQQI 306 AVYWYCRSLPFTLLP+ CSLVSLVWQFGTL LLGYGLDPLAVLVPFLVFAIGVSHGVQQI Sbjct: 247 AVYWYCRSLPFTLLPVACSLVSLVWQFGTLRLLGYGLDPLAVLVPFLVFAIGVSHGVQQI 306 Query: 307 NYIVRGIAAGMGCDEAARASFRGLLVPGVLALVTAFVSFITLTLIPIPMVKELAITASIG 366 N IVRGIA+G C+ AARASF+GLL+PGVLALVTA VSF+TL +IPIPMV+ELAITASIG Sbjct: 307 NTIVRGIASGQSCEAAARASFKGLLIPGVLALVTALVSFVTLVMIPIPMVRELAITASIG 366 Query: 367 VGYKIITNLVMLPLAASLLKVDKAYADAAMRAQQKRARWLQVFARAAHPRNARGIVLVTL 426 VGYKIITNLVMLPLAASLLKV K YADAAMRAQQ R RWL V +R+A PR AR IV++TL Sbjct: 367 VGYKIITNLVMLPLAASLLKVGKTYADAAMRAQQARGRWLAVLSRSAQPRVARSIVVITL 426 Query: 427 LLFAASVWYSSDRLVGTLQAGAPELRADSRYNLDAKAIAENYDTGLDWLSVILATPENSC 486 L+ A S WYS DRLVGTLQ GAPEL AD+RYN DA+ IA+NY GLDWL+VILATP NSC Sbjct: 427 LVLAGSAWYSHDRLVGTLQPGAPELHADARYNRDARWIADNYGNGLDWLTVILATPANSC 486 Query: 487 DQVAFGRYQDQFDAAMAQVPGVMSVSSFAQQMKIYNEGYNEGNPAMYSVPLDPANYAALA 546 DQV FGRYQDQFD M VPGV+SVSSFA QMKIYNEGYNEGNPAM++VPLD NYAALA Sbjct: 487 DQVRFGRYQDQFDTVMRHVPGVLSVSSFADQMKIYNEGYNEGNPAMFTVPLDAGNYAALA 546 Query: 547 VEIGRVRGFLSKDCSMLATHLYLTDHKASTINGVIAAVKQYRSAHPLPGLSIRLAAGNAG 606 VEIGRVRGFLSKDC+MLA+HL+L DHKA+TINGVI AVK+YR H L GL IRLAAGNAG Sbjct: 547 VEIGRVRGFLSKDCNMLASHLFLADHKAATINGVIQAVKRYRDQHKLEGLEIRLAAGNAG 606 Query: 607 VIAAVNEEVEKSELPMMLYVYAAIVVLVFAVYRDLRAVVACCLPLTVATFIGYAFMKQLQ 666 VIAAVN+EVEKSELPMMLYVY AIV+LVFAVYRD RA++ACCLPLTVATFIGYAFMKQLQ Sbjct: 607 VIAAVNDEVEKSELPMMLYVYGAIVLLVFAVYRDWRAIIACCLPLTVATFIGYAFMKQLQ 666 Query: 667 IGLTVATLPVMVLAVGIGVDYAFYIYSRLQMHLAAGEAIDVALEKSIQEIGVATIFTAIT 726 IGLTVATLPVMVLAVGIGVDYAFYIY+RLQ+HLAAG AI+ AL +IQE+G+ATIFTAIT Sbjct: 667 IGLTVATLPVMVLAVGIGVDYAFYIYNRLQLHLAAGLAIEEALHNAIQEVGIATIFTAIT 726 Query: 727 LAIGVATWSFSQLKFQADMGKLLAFMFIVNLIMAMTALPAFAVVLERLFPRKGKIKVNSM 786 LAIGVATWSFS LKFQADMGKLLAFMF+VNLIMAMTALPAFAVVL+++FPRK IK+NS Sbjct: 727 LAIGVATWSFSDLKFQADMGKLLAFMFLVNLIMAMTALPAFAVVLDKMFPRKSPIKLNSA 786 Query: 787 MSH 789 ++H Sbjct: 787 LAH 789 >gi|300310853|ref|YP_003774945.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1078 Score = 65.1 bits (157), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 53/177 (29%), Positives = 91/177 (51%), Gaps = 7/177 (3%) Query: 238 VMALLLTALAVYWYCRSLPFTLLPIVCSLVSLVWQFGTLHLLGYGLDPLAVLVPFLVFAI 297 ++++ L + V+ + R+ TL+P V VSL+ FG ++L G+ LD L+++ + Sbjct: 339 ILSVALVIMVVFLFLRNARATLIPAVAVPVSLIGTFGVMYLAGFSLDNLSLMALTIATGF 398 Query: 298 GVSHGVQQINYIVRGIAAGMGCDEAARASFR--GLLVPGV-LALVTAFVSFITLTLIPIP 354 V + + I R I +GM +AA R G V + ++L+ F+ + + I Sbjct: 399 VVDDAIVVLENISRHIESGMKPYKAALLGAREVGFTVLSMSISLIAVFIPILMMEGIVGR 458 Query: 355 MVKELAITASIGVGYKIITNLVMLP-LAASLLKVDKAYADAAMRAQQKRARWLQVFA 410 + +E AIT S+ + ++ +L P + A LL+ D A AD A R Q+R WLQ A Sbjct: 459 LFREFAITLSVAILVSLVVSLTTTPMMCAQLLRHDPA-ADEARR--QRRHPWLQKIA 512 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.138 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,277,243 Number of extensions: 93539 Number of successful extensions: 422 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 418 Number of HSP's successfully gapped: 3 Length of query: 789 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 696 Effective length of database: 1,179,515 Effective search space: 820942440 Effective search space used: 820942440 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 134 (56.2 bits)