BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0171.p (417 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313229|ref|YP_003777321.1| 4-aminobutyrate aminotransferas... 804 0.0 gi|300310415|ref|YP_003774507.1| 4-aminobutyrate aminotransferas... 208 8e-55 gi|300312816|ref|YP_003776908.1| aminotransferase class III prot... 163 3e-41 gi|300310456|ref|YP_003774548.1| family II aminotransferase [Her... 140 2e-34 gi|300312624|ref|YP_003776716.1| acetylornithine aminotransferas... 137 1e-33 >gi|300313229|ref|YP_003777321.1| 4-aminobutyrate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 456 Score = 804 bits (2076), Expect = 0.0, Method: Compositional matrix adjust. Identities = 390/398 (97%), Positives = 394/398 (98%) Query: 1 MIEAELESLNFADAPKMVSSTYPGPKTAAALELSARTESMARGGGRMPIAMDQAFGVTFK 60 MIEAELESLNF DAPKMV+ TYPGPKTAAALELSARTESMARGGGRMPIAMDQAFGVTFK Sbjct: 1 MIEAELESLNFTDAPKMVTPTYPGPKTAAALELSARTESMARGGGRMPIAMDQAFGVTFK 60 Query: 61 DPDGNTFIDLSAGVGVSSVGRCNPRVVEAIRKQSESLMHSMEVNSSKRTELAAKISEIMP 120 DPDGNTFIDLSAGVGVSSVGRCNPRVVEAIRKQSESLMHSMEVNSSKRTELAAKISEIMP Sbjct: 61 DPDGNTFIDLSAGVGVSSVGRCNPRVVEAIRKQSESLMHSMEVNSSKRTELAAKISEIMP 120 Query: 121 EGLRGDCITFFTQGGSDALEAAVKFAKRVTGRHQIIAFHGGYHGIWNASNALTTGTAYRK 180 +GLRGDCITFFTQGGSDALEAAVKFAKRVTGRHQIIAFHGGYHGIWNASNALTTGTAYRK Sbjct: 121 DGLRGDCITFFTQGGSDALEAAVKFAKRVTGRHQIIAFHGGYHGIWNASNALTTGTAYRK 180 Query: 181 GFGPFMGGVIHAPYPYAYRFPFDTSNKSAEQIAGEYVDYLLNTPYTAADDVAAVIVEPVQ 240 GFGPFMGGVIHAPYPYAYRFPFDTS+KSAEQIAGEYVDYLLNTPYTAADDVAAVIVEPVQ Sbjct: 181 GFGPFMGGVIHAPYPYAYRFPFDTSHKSAEQIAGEYVDYLLNTPYTAADDVAAVIVEPVQ 240 Query: 241 GEGGYVPPSPEFLQILRKACDRSGALLIVDEVQAGAGRTGKMWAVEHSGVKPDMLTFGKG 300 GEGGYVPPSPEFLQILRKACDRSGALLIVDEVQAGAGRTGKMWAVEHSGVKPDMLTFGKG Sbjct: 241 GEGGYVPPSPEFLQILRKACDRSGALLIVDEVQAGAGRTGKMWAVEHSGVKPDMLTFGKG 300 Query: 301 IGGDMPMAGLVMRSDLAAKIPDGSQPNTFAANSISAAVALTNISILQDPRLDLVNRAHAL 360 IGGDMPMAGLVMRSDLAAKIPDGSQPNTFAANSISAAVALTNISILQDPRLDLVNRAH L Sbjct: 301 IGGDMPMAGLVMRSDLAAKIPDGSQPNTFAANSISAAVALTNISILQDPRLDLVNRAHTL 360 Query: 361 GLEAQERIRSFNSHWVGEVRGRGLMIGIELVENRETRD 398 GLEAQERIRSFNS WVGEVRGRGLMIGIELVENRETR+ Sbjct: 361 GLEAQERIRSFNSPWVGEVRGRGLMIGIELVENRETRE 398 >gi|300310415|ref|YP_003774507.1| 4-aminobutyrate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 426 Score = 208 bits (529), Expect = 8e-55, Method: Compositional matrix adjust. Identities = 127/337 (37%), Positives = 191/337 (56%), Gaps = 17/337 (5%) Query: 61 DPDGNTFIDLSAGVGVSSVGRCNPRVVEAIRKQSESLMHSME--VNSSKRTELAAKISEI 118 D +G FID +AG+ V + G +P++++A+R Q + H+ V + ELA +I+ + Sbjct: 40 DVEGRRFIDFAAGIAVLNTGHRHPKLLDAMRAQMDKFTHTAYQIVPYASYVELAERINRL 99 Query: 119 MPEGLRGDCITFFTQGGSDALEAAVKFAKRVTGRHQIIAFHGGYHGIWNASNALTTGTA- 177 P G FF+ G ++A+E A+K A+ TGR +IAF GG+HG ALT A Sbjct: 100 TP-GNYPKKTAFFSTG-AEAVENAIKIARAHTGRPGVIAFAGGFHGRTMMGMALTGKVAP 157 Query: 178 YRKGFGPFMGGVIHAPYPYAYRFPFDTSNKSAEQIAGEYVDYLLNTPYTAADDVAAVIVE 237 Y+ GFGPF G V HAPYP A TS + E + G + + A VAA+I+E Sbjct: 158 YKLGFGPFPGDVFHAPYPSALHG--ITSEDALEAVKGLFKSDI------EAKRVAAIILE 209 Query: 238 PVQGEGGYVPPSPEFLQILRKACDRSGALLIVDEVQAGAGRTGKMWAVEHSGVKPDMLTF 297 PVQGEGG+ +F++ LR CD G LLI DEVQ+G GRTGK++A+EH V PD++T Sbjct: 210 PVQGEGGFYAAPADFMRGLRALCDEHGILLIADEVQSGYGRTGKLFAMEHYDVLPDLMTM 269 Query: 298 GKGIGGDMPMAGLVMRSDLAAKIPDGSQPNTFAANSISAAVALTNISILQDPRLDLVNRA 357 K + G MP++ + R+++ G T+A N ++ A AL + ++++ + LV R Sbjct: 270 AKSLAGGMPLSAVNGRAEIMDAPAPGGLGGTYAGNPLAIASALAVLDVMEEEQ--LVTRG 327 Query: 358 HALGLEAQERIRSFNSH--WVGEVRGRGLMIGIELVE 392 LG + QE ++ S + EVRG G M+ +E + Sbjct: 328 QRLGDKLQEHLKELRSSVPQIAEVRGVGAMVAVEFAD 364 >gi|300312816|ref|YP_003776908.1| aminotransferase class III protein [Herbaspirillum seropedicae SmR1] Length = 440 Score = 163 bits (412), Expect = 3e-41, Method: Compositional matrix adjust. Identities = 124/370 (33%), Positives = 190/370 (51%), Gaps = 27/370 (7%) Query: 46 RMPIAMDQAFGVTFKDPDGNTFIDLSAGVGVSSVGRCNPRVVEAIRKQSESLM--HSMEV 103 ++P+A+ G+ D DG +ID S G VS +G +PRV+EAIRKQ L H+ Sbjct: 12 KLPMAV-AGEGMELIDQDGKRYIDASGGAAVSCLGHGHPRVIEAIRKQVGELAYAHTSFF 70 Query: 104 NSSKRTELAAKISEIMPEGLRGDCITFFTQGGSDALEAAVKFAKRVT------GRHQIIA 157 ++ ELAA +++ P L +F GGS+A+EAA+K A++ R IIA Sbjct: 71 TTAPAEELAAMLADAAPGSLNH---VYFLSGGSEAVEAALKLARQYYVEVGQPQRRHIIA 127 Query: 158 FHGGYHGIWNASNALTTG-TAYRKG-FGPFMGGVIHAPYPYAYRFPFDTSN--KSAEQIA 213 YHG N AL G A+R+ F P + H YAYR D + + +++A Sbjct: 128 RRQSYHG--NTLGALAIGGNAWRREMFMPMLIEAHHVSPCYAYRNRADGESDVQYVQRLA 185 Query: 214 GEYVDYLLNTPYTAADDVAAVIVEPVQG-EGGYVPPSPEFLQILRKACDRSGALLIVDEV 272 E +L+ AD V A + E V G G VPP ++ + +R CD+ G LLI+DEV Sbjct: 186 DELEQKILSL---GADQVIAFVAETVVGATAGAVPPVGDYFRKIRAVCDKYGVLLILDEV 242 Query: 273 QAGAGRTGKMWAVEHSGVKPDMLTFGKGIG-GDMPMAGLVMRSDLAAKIPDGS----QPN 327 +G GRTG ++A E GV PD++ KG+G G P+ ++ + + GS + Sbjct: 243 MSGMGRTGYLFACEEDGVVPDIVVIAKGLGAGYQPIGAMICSDHIYDAVLRGSGFFQHGH 302 Query: 328 TFAANSISAAVALTNISILQDPRLDLVNRAHALGLEAQERIRSFNSHWVGEVRGRGLMIG 387 T+ ++ + A A+ +Q+ RL R L ++ R + VG++RGRGL +G Sbjct: 303 TYIGHATACAAAVAVQKTIQEERLLENVRQRGEQLRSELRQAFGDQAHVGDIRGRGLFVG 362 Query: 388 IELVENRETR 397 +ELV R ++ Sbjct: 363 VELVAERSSK 372 >gi|300310456|ref|YP_003774548.1| family II aminotransferase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 140 bits (353), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 108/371 (29%), Positives = 174/371 (46%), Gaps = 28/371 (7%) Query: 48 PIAMDQAFGVTFKDPDGNTFIDLSAGVGVSSVGRCNPRVVEAIRKQSESLMHS---MEVN 104 P+ + G D +D +G+ + G V+ A +Q + L ++ Sbjct: 29 PLILKSGQGAYLTDHQDKQLLDAFSGLWCVNTGYGQESVIRAATEQMQRLPYATGYFHFA 88 Query: 105 SSKRTELAAKISEIMPEGLRGDCITFFTQGGSDALEAAVKFAKRVT------GRHQIIAF 158 S LA K+ +I P L+ +FT GGSDA++AA+++ G+ I+ Sbjct: 89 SEPAILLAKKLVDISPASLQH---VYFTLGGSDAVDAAIRYITHYHHSIGKPGKQHFISL 145 Query: 159 HGGYHGIWNASNALTTGTAYRKGFGPFMGGVIHAPYPYAYRFPFDTSNKSAEQIAGEYVD 218 GYHG + LT + F + + P PY YR + + E I V Sbjct: 146 QRGYHGSSSVGAGLTALPNFHYHFNLPLPTQHYLPSPYPYR----SELQGDEAIIAASVQ 201 Query: 219 YLLN-TPYTAADDVAAVIVEPVQGEGGYVPPSPEFLQILRKACDRSGALLIVDEVQAGAG 277 L + AD+VAA EP+QG GG V P +L+ ++ AC+ L +VDEV G G Sbjct: 202 ALRDKVAELGADNVAAFFCEPIQGSGGVVVPPKGWLKAMQLACNELDILFVVDEVITGFG 261 Query: 278 RTGKMWAVEHSGVKPDMLTFGKGI-GGDMPMAGLVMRSDLAAKIPDGS-------QPNTF 329 RTG M+A V+PD++T KG+ G PM ++M + I DG+ T+ Sbjct: 262 RTGPMFACLDEDVQPDLMTMAKGLTAGYAPMGAVMMSDRVYNGIADGAPMSTVVGHGQTY 321 Query: 330 AANSISAAVALTNISILQDPRLDLVNRAHALGLEAQERIRSFNSH-WVGEVRGRGLMIGI 388 +A+ +SAAVAL + + ++ L++ L +R+ +H VG+ R RGL+ + Sbjct: 322 SAHPVSAAVALEVLRLYEEG--GLLDNGRRLEPVFAGGLRALLAHPLVGDARSRGLLGAL 379 Query: 389 ELVENRETRDR 399 ELV +++T+ R Sbjct: 380 ELVADKQTKAR 390 >gi|300312624|ref|YP_003776716.1| acetylornithine aminotransferase [Herbaspirillum seropedicae SmR1] Length = 400 Score = 137 bits (346), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 104/356 (29%), Positives = 171/356 (48%), Gaps = 42/356 (11%) Query: 46 RMPIAMDQAFGVTFKDPDGNTFIDLSAGVGVSSVGRCNPRVVEAIRKQSESLMHSMEVNS 105 R + + G+ D +G ++D G V+++G + +A+ QS+ L++ + Sbjct: 17 RPELVFTEGHGMWLTDHNGKRYLDYLQGWAVNTLGHAPQCIADALAAQSKKLINP---SP 73 Query: 106 SKRTELAAKISEIMPEGLRGDCITFFTQGGSDALEAAVKFAKRV---------TGRHQII 156 + E + ++++++ D + FF G +A E A+K A++ + R +II Sbjct: 74 AFYNEPSIELAKLLTANSVFDRV-FFANSGGEANEGAIKLARKWGKKNPAADGSARFEII 132 Query: 157 AFHGGYHGIWNASNALTTGTAYRKGFGPFMGGVIHAPYPYAYRFPFDTSN--KSAEQIAG 214 F +HG A+ + + + F P + G FP N +S + + G Sbjct: 133 TFKHSFHGRTLATMSASGKDGWDTMFAPQVPG-----------FPKAVLNDLESVKALIG 181 Query: 215 EYVDYLLNTPYTAADDVAAVIVEPVQGEGGYVPPSPEFLQILRKACDRSGALLIVDEVQA 274 E+ AV++EPVQGEGG +P S EF+Q LR LLIVDEVQ+ Sbjct: 182 EHT--------------VAVMLEPVQGEGGVIPASKEFMQGLRSLTKEKNLLLIVDEVQS 227 Query: 275 GAGRTGKMWAVEHSGVKPDMLTFGKGIGGDMPMAGLVMRSDLAAKIPDGSQPNTFAANSI 334 G GRTG+++A +HSG++PD++T KGIGG +P+A L+ R ++A G Q T+ N + Sbjct: 228 GMGRTGQLFAYQHSGIEPDIMTLAKGIGGGVPLAALLAREEIAC-FEAGEQGGTYNGNPL 286 Query: 335 SAAVALTNISILQDPRLDLVNRAHALGLEAQERIRSFNSHWVGEVRGRGLMIGIEL 390 AV + I L P R L + S + GE RG GL+ ++L Sbjct: 287 MTAVGVAVIKELLKPGFMESVRERGQYLRQRSLEISEKYGFEGE-RGEGLLRALQL 341 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.133 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,321,422 Number of extensions: 58816 Number of successful extensions: 231 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 206 Number of HSP's successfully gapped: 10 Length of query: 417 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 329 Effective length of database: 1,203,190 Effective search space: 395849510 Effective search space used: 395849510 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)