BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0169.p (404 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313230|ref|YP_003777322.1| methyl-accepting chemotaxis I (... 538 e-154 gi|300310474|ref|YP_003774566.1| methyl-accepting chemotaxis tra... 198 6e-52 gi|300311614|ref|YP_003775706.1| methyl-accepting chemotaxis tra... 198 8e-52 gi|300312038|ref|YP_003776130.1| methyl-accepting chemotaxis tra... 187 1e-48 gi|300313024|ref|YP_003777116.1| methyl-accepting chemotaxis pro... 187 1e-48 >gi|300313230|ref|YP_003777322.1| methyl-accepting chemotaxis I (serine chemoreceptor protein) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 535 Score = 538 bits (1386), Expect = e-154, Method: Compositional matrix adjust. Identities = 321/381 (84%), Positives = 349/381 (91%) Query: 1 MRQALAALSIAARLRLAFALLLLMLVGMALFSYGRMSHINRQVEQLGSINVPAMESVSGI 60 MRQ A SIAARLRLAF LLLLML+GMA+FSY RMS IN QV+QLG INVPAMESVSGI Sbjct: 1 MRQWFAGFSIAARLRLAFCLLLLMLIGMAVFSYDRMSRINTQVDQLGRINVPAMESVSGI 60 Query: 61 RIGMAQYRMGVTLALLADDGSGGTAAAQARREGLAYAEKYLKILVDFASNDDERATSRQL 120 RIGMAQYRMGVTL+LL DDGSGG AA +ARR+GLAYAEKYL+ILVDFASNDDERATSRQL Sbjct: 61 RIGMAQYRMGVTLSLLPDDGSGGKAAEEARRDGLAYAEKYLQILVDFASNDDERATSRQL 120 Query: 121 SASWQAYRALAEKAVALAAAGQSAEALLLAADSAAPYEQMAGILKFISDSVAQESAASVA 180 ASW+AYR LA KAVALA AGQS+EAL+LAADSA PYEQMA ILKFISDSV+QESAASV Sbjct: 121 VASWKAYRELAGKAVALAQAGQSSEALMLAADSAQPYEQMASILKFISDSVSQESAASVV 180 Query: 181 DGQQAFVSARHALIAVSVAALLGTLVLARLLIGGIAGPLRRAVQAAHRIAEGALDQEITV 240 DG+QA+ SA AL+AVSVAALL TL LARLLI GIA PLRRAV+AA+RIAEGALDQ+I V Sbjct: 181 DGEQAYRSAWRALLAVSVAALLCTLGLARLLIRGIATPLRRAVEAANRIAEGALDQDIVV 240 Query: 241 SGRDEISQLLTAMQRMQQALVGIVAGVRREAEHVATASVQIAAGNADLSTRTEAQAAALE 300 GRDEI+QLL+AMQRMQ ALVGIVAGVRREAEHVATASV+IA+GNADLSTRTEAQAAALE Sbjct: 241 HGRDEIAQLLSAMQRMQHALVGIVAGVRREAEHVATASVEIASGNADLSTRTEAQAAALE 300 Query: 301 ETAATMNELSATVQKNAEDARQASGTARQASTVALQGGEVVQEVVQTMRGIEDSSQRISE 360 ETAATMNEL+ATVQKNA+DAR+AS TA+QAS VALQGG+VVQ+VVQTMRGIEDSSQRISE Sbjct: 301 ETAATMNELNATVQKNADDAREASSTAQQASAVALQGGQVVQQVVQTMRGIEDSSQRISE 360 Query: 361 IVGTIDGIAFQTNILALXAAL 381 IVGTIDGIAFQTNILAL AA+ Sbjct: 361 IVGTIDGIAFQTNILALNAAV 381 >gi|300310474|ref|YP_003774566.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 198 bits (504), Expect = 6e-52, Method: Compositional matrix adjust. Identities = 139/375 (37%), Positives = 210/375 (56%), Gaps = 1/375 (0%) Query: 8 LSIAARLRLAFALLLLMLVGMALFSYGRMSHINRQVEQLGSINVPAMESVSGIRIGMAQY 67 L+IA +L L+FA+++ + + LF+ + +N+ ++ S +P M++ ++ + Sbjct: 7 LNIAKKLALSFAVVIALTLLQGLFAIKELKQVNKASGEIVSKWMPTMDAARELQGSLPPL 66 Query: 68 RMGVTLALLADDGSGGTAAAQARREGLAYAEKYLKILVDFASNDDERATSRQLSASWQAY 127 R+ + A AA +A ++ L + I S E+ Q + AY Sbjct: 67 RLNELELVTAVTPQERAAANKAIKDRLDALTRQRAIFEQRMSEPQEKEQYAQFLKNLTAY 126 Query: 128 RALAEKAVALAAAGQSAEA-LLLAADSAAPYEQMAGILKFISDSVAQESAASVADGQQAF 186 + E+ +AA Q E L DSA Y M G L I SA + + F Sbjct: 127 VKVDEQLAEMAANQQYDETRALFNGDSAKLYRSMVGNLDAIVKINTSGSAQADEEASTVF 186 Query: 187 VSARHALIAVSVAALLGTLVLARLLIGGIAGPLRRAVQAAHRIAEGALDQEITVSGRDEI 246 A+ ++ + A +L +LA L+ ++ PL+ AVQ A R+A G L I +GRDE Sbjct: 187 RGAQRWIVGLLAATVLIASLLAFLVATNVSRPLKEAVQIAQRVARGDLTLRIRPAGRDET 246 Query: 247 SQLLTAMQRMQQALVGIVAGVRREAEHVATASVQIAAGNADLSTRTEAQAAALEETAATM 306 L+ A++ M ++L IV+ VR+ + + TAS +IA GN DLS RTE QA +LEETA+ M Sbjct: 247 GALMEALRAMNESLRDIVSEVRQGTDTITTASHEIARGNLDLSNRTEQQAGSLEETASAM 306 Query: 307 NELSATVQKNAEDARQASGTARQASTVALQGGEVVQEVVQTMRGIEDSSQRISEIVGTID 366 EL++TV +NA++ARQA+ A AS +A QGGEVV +VVQTM GI +SS+RI++I+ ID Sbjct: 307 EELTSTVTQNADNARQANQLAATASDIASQGGEVVGQVVQTMEGITESSRRIADIISVID 366 Query: 367 GIAFQTNILALXAAL 381 GIAFQTNILAL AA+ Sbjct: 367 GIAFQTNILALNAAV 381 >gi|300311614|ref|YP_003775706.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 581 Score = 198 bits (503), Expect = 8e-52, Method: Compositional matrix adjust. Identities = 143/396 (36%), Positives = 220/396 (55%), Gaps = 42/396 (10%) Query: 8 LSIAARLRLAFALLLLMLVGMALFSYGRMSHINRQVEQLGSINVPAMESVSGIRIGMAQ- 66 L +A RL FA ++ +L +++ ++ ++++INR ++Q+ + + +R G+ + Sbjct: 8 LKVAVRLAAVFAFIVALLAVVSVTAFIKINNINRAIDQIVNDRYLKVRWAFDLRDGVNEQ 67 Query: 67 --YRMGVTLALLADDGSGGTAAAQARREGLAYAEKYLKILVDFASNDDERATSRQLSASW 124 Y G+ + + A + R L A K K +D + T+RQ++A Sbjct: 68 IKYLRGIAIDI------ENRPANEKRFAQLEVAVKQTKEALD-------KITARQVTAVG 114 Query: 125 QAY-RALAEKAVALAAAGQSAEALLLAADSAAPYEQMAGILKFISDS------------- 170 Q +AL E +A A+ AL+ A +S + E +L+ I+D+ Sbjct: 115 QKKAKALDEARIAFDASKADFLALVKAGNSTSADEY---VLRKITDTQNNYLALARAFAD 171 Query: 171 -----VAQESAASVADGQQAFVSARHALIAVSVAALLGTLVLARLLIGGIAGPLRRAVQA 225 + E A +VADG A +A S+AA+L ++L L+ I PL AV+ Sbjct: 172 SQDAQLQAEGAKAVADGAMAI----EVTLACSMAAVLAAIILGYLMARSIVKPLTEAVRV 227 Query: 226 AHRIAEGALDQEITVSGRDEISQLLTAMQRMQQALVGIVAGVRREAEHVATASVQIAAGN 285 A +A G L I RDE QL+ A+++M L+ IV+GVRR E + TAS +IA+GN Sbjct: 228 AENVAAGDLTTRIEPHSRDETGQLMQALRKMNDNLIEIVSGVRRGTESITTASGEIASGN 287 Query: 286 ADLSTRTEAQAAALEETAATMNELSATVQKNAEDARQASGTARQASTVALQGGEVVQEVV 345 DLS RTE QA +LEETA+ M E+++TV++NA++ARQA+ A AS +ALQGG++V VV Sbjct: 288 LDLSARTEQQAGSLEETASAMEEMTSTVRQNADNARQANQMAVSASEIALQGGQIVGRVV 347 Query: 346 QTMRGIEDSSQRISEIVGTIDGIAFQTNILALXAAL 381 TM I SS++I +I+G IDGIAFQTNILAL AA+ Sbjct: 348 HTMDEINQSSRKIVDIIGVIDGIAFQTNILALNAAV 383 >gi|300312038|ref|YP_003776130.1| methyl-accepting chemotaxis transmembrane I protein [Herbaspirillum seropedicae SmR1] Length = 579 Score = 187 bits (476), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 138/384 (35%), Positives = 217/384 (56%), Gaps = 19/384 (4%) Query: 8 LSIAARLRLAFALLLLMLVGMALFSYGRMSHINRQVEQLGSINVPAMESVSGIRIGMAQY 67 L I+ +L F +++ M+ + +FS ++ +N ++ + +P++ +++ +++ M++ Sbjct: 7 LRISRKLITTFLVVVAMVAALGVFSIRQLDQVNSASTEISTNWLPSIRTLARLQLAMSRL 66 Query: 68 RMGVTLALLADDGSGGTAAAQARREGLAYAEKYLKI----LVDFASNDDERATSRQLSAS 123 R +LA D + G+ LK V S +E+A ++ Sbjct: 67 RSFELQHILAKDAK----EYEEVERGMQVQMNNLKTAQAEYVTQISEPEEKAIYPEVEKL 122 Query: 124 WQAYRALAEKAVALAAAGQSAEALLLA-ADSAAPYE---QMAGILKFISDSVAQESAASV 179 + + +K +AL+ G+ EA L +S Y Q L ++D A + Sbjct: 123 IATFVSEHDKIIALSRQGKDEEARNLQRGESTKAYRTSLQQIDKLAAVNDK-----GADI 177 Query: 180 ADGQQAFVSARHAL-IAVSVAALLG-TLVLARLLIGGIAGPLRRAVQAAHRIAEGALDQE 237 ++ Q + A+ L I V+VAA +G ++LA + IA PL AV A R+A G L Sbjct: 178 SNKQADAIYAQARLWIIVTVAACIGLAMLLAVWVSRLIARPLEEAVSVAQRVAGGDLTTR 237 Query: 238 ITVSGRDEISQLLTAMQRMQQALVGIVAGVRREAEHVATASVQIAAGNADLSTRTEAQAA 297 I +G+DE QLL A++ M +LVGIV VR + + TAS +IA+GN DLS+RTE QA+ Sbjct: 238 IEPAGKDETGQLLVALKSMNDSLVGIVGQVRMGTQTITTASTEIASGNLDLSSRTEEQAS 297 Query: 298 ALEETAATMNELSATVQKNAEDARQASGTARQASTVALQGGEVVQEVVQTMRGIEDSSQR 357 +LEETA+ M EL++TV++NA++ARQA+ A AS VA QGG VV +VV TM I SS++ Sbjct: 298 SLEETASAMEELTSTVKQNADNARQANQLAVSASEVASQGGAVVNQVVDTMGSINASSRK 357 Query: 358 ISEIVGTIDGIAFQTNILALXAAL 381 I++I+G IDGIAFQTNILAL AA+ Sbjct: 358 IADIIGVIDGIAFQTNILALNAAV 381 >gi|300313024|ref|YP_003777116.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 571 Score = 187 bits (475), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 130/375 (34%), Positives = 211/375 (56%), Gaps = 1/375 (0%) Query: 8 LSIAARLRLAFALLLLMLVGMALFSYGRMSHINRQVEQLGSINVPAMESVSGIRIGMAQY 67 L IA +L AF ++L+ ++G+ FS ++ +N+ + +P+++++S + + +A+ Sbjct: 7 LKIAHKLNAAFLVVLVFIIGLGGFSSLELAAVNKSSTDIARNWLPSIDALSKMSLALARS 66 Query: 68 RMGVTLALLADDGSGGTAAAQARREGLAYAEKYLKILVDFASNDDERATSRQLSASWQAY 127 R LL D A+ + + +A K + S E+ ++ + Y Sbjct: 67 RSFDMQQLLTADKQEEKASRERADKQMATLLKEVARYGKLVSEPREKELYPEIERNIALY 126 Query: 128 RALAEKAVALAAAGQSAEALLLAADSAAP-YEQMAGILKFISDSVAQESAASVADGQQAF 186 + E+ AL Q A L ++ P Y ++ + +S+ ++ +A S AD + Sbjct: 127 KEAHERMSALFEQDQKQAAHALMNSTSTPVYRKLQEQIGELSEVNSKGAALSDADADHIY 186 Query: 187 VSARHALIAVSVAALLGTLVLARLLIGGIAGPLRRAVQAAHRIAEGALDQEITVSGRDEI 246 A A++A+ ++ + L+ + +A PL AV A ++A G L +I + RDE Sbjct: 187 GQANMAILALVTTCVVLAMALSWWIARLVARPLANAVDIARQVAAGDLTADIPTAHRDET 246 Query: 247 SQLLTAMQRMQQALVGIVAGVRREAEHVATASVQIAAGNADLSTRTEAQAAALEETAATM 306 QLL A+++M L+ IV VR+ + + TAS +IA GN DLS+RTE QA ALEETA+ M Sbjct: 247 GQLLEALRQMNGNLLNIVREVRQGTDTITTASAEIATGNMDLSSRTEQQAGALEETASAM 306 Query: 307 NELSATVQKNAEDARQASGTARQASTVALQGGEVVQEVVQTMRGIEDSSQRISEIVGTID 366 EL++TV++N+++ARQA+ A AS VA GG VV +VV TM I DSS++I +I+G ID Sbjct: 307 EELTSTVRQNSDNARQANQLAVNASEVAQAGGTVVGKVVATMSAINDSSRKIVDIIGVID 366 Query: 367 GIAFQTNILALXAAL 381 GIAFQTNILAL AA+ Sbjct: 367 GIAFQTNILALNAAV 381 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.127 0.346 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 863,974 Number of extensions: 28151 Number of successful extensions: 305 Number of sequences better than 1.0e-08: 66 Number of HSP's gapped: 295 Number of HSP's successfully gapped: 66 Length of query: 404 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 317 Effective length of database: 1,207,925 Effective search space: 382912225 Effective search space used: 382912225 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)