BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0168.p (480 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrog... 782 0.0 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 533 e-153 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 379 e-106 gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semial... 315 5e-87 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 280 2e-76 >gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 480 Score = 782 bits (2020), Expect = 0.0, Method: Compositional matrix adjust. Identities = 417/479 (87%), Positives = 447/479 (93%) Query: 1 MYPDLQLYINGRFHGLDGRRYQEVRDPATGDVLGRLPWAEAGDIEEAIQAAHAAFQQWRK 60 MYPDLQLYI+GRFHG++GRRYQEVRDPATGDVLGRLPWAE DIEEAIQAAH AFQQWR+ Sbjct: 1 MYPDLQLYIDGRFHGVEGRRYQEVRDPATGDVLGRLPWAERADIEEAIQAAHRAFQQWRQ 60 Query: 61 VSPLERSDILRRAAALTRERAQAMAPAITMDNGKPLADAVAEILNAAEHLDWHAEEGRRL 120 VSPLERSDILRRAAAL RERAQAMAPAITMDNGKPLADAVAEI+NAAEHLDWHAEEGRRL Sbjct: 61 VSPLERSDILRRAAALARERAQAMAPAITMDNGKPLADAVAEIINAAEHLDWHAEEGRRL 120 Query: 121 YGRVVGARHPEVRQLVVREPVGVCAAISPWNFPFGQAMKKVAAAMAAGCTVVLKGPSESP 180 YGRVVGARHPEVRQLVVREPVGVCAAISPWNFPFGQAMKKVAAAMAAGCTVVLKGPSESP Sbjct: 121 YGRVVGARHPEVRQLVVREPVGVCAAISPWNFPFGQAMKKVAAAMAAGCTVVLKGPSESP 180 Query: 181 AAIVLMAQLFHDAGVPAGVFNIVWGVSSEISQQLIASPLVRTISFTGSIPVGSQLAAQAA 240 AAIVLMAQLFHDAGVPAGVFNIV GVSSEISQ LI SPLVR ISFTGS+PVGS+LAAQAA Sbjct: 181 AAIVLMAQLFHDAGVPAGVFNIVSGVSSEISQHLIESPLVRNISFTGSVPVGSKLAAQAA 240 Query: 241 LHMKRMSMELGGHAPVLVFDDAEVDAAARLLARLKIRNAGQVCVSPSRFFVHQRIYDRFV 300 LHMKRMSMELGGHAPVLVFDDAEV+ AARLLARLK RNAGQVCVSPSRF+V + IY RFV Sbjct: 241 LHMKRMSMELGGHAPVLVFDDAEVEPAARLLARLKTRNAGQVCVSPSRFYVQKGIYARFV 300 Query: 301 VALADALQQVKVGSGLEAGVQMGPLIHGRRFDAVHGLIEDAVSQGAQLVTGGRRIGERGH 360 A A+AL Q+KVG G E GVQMGPLIHGRRF+AV GLI+DA++QG++LVTGG R+G+RGH Sbjct: 301 TAFAEALLQIKVGPGHEPGVQMGPLIHGRRFEAVQGLIDDALAQGSELVTGGARLGQRGH 360 Query: 361 FHAPTLLADVPPTARVMQEEPFGPVAAVTPFAHTAQALAMANALPLGLASYVFTQSLGTA 420 F+APTL+ VPP+ARVMQEEPFGP+AAVTPF+ +A+AMANALPLGLASYVFTQSL TA Sbjct: 361 FYAPTLITGVPPSARVMQEEPFGPIAAVTPFSQAEEAIAMANALPLGLASYVFTQSLDTA 420 Query: 421 HEVASGLQAGMVNINHSGMAHPELPFGGIGESGFGSEGGTESFDSFLVTKMISQISHVP 479 H V+ LQAGMVNINHSGMAHPELPFGGIGESGFGSEGG E F+SFLVTKMISQI+HVP Sbjct: 421 HRVSRDLQAGMVNINHSGMAHPELPFGGIGESGFGSEGGVEGFESFLVTKMISQITHVP 479 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 533 bits (1374), Expect = e-153, Method: Compositional matrix adjust. Identities = 250/476 (52%), Positives = 348/476 (73%) Query: 1 MYPDLQLYINGRFHGLDGRRYQEVRDPATGDVLGRLPWAEAGDIEEAIQAAHAAFQQWRK 60 MY D+QL+ING + R +V +PAT +V+G++ A+ D++ A++AA F+ WR Sbjct: 1 MYKDVQLFINGEWTASASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRN 60 Query: 61 VSPLERSDILRRAAALTRERAQAMAPAITMDNGKPLADAVAEILNAAEHLDWHAEEGRRL 120 +S ERS I+RRAA L RERA+ +A +TM+ GKPLA+A E L AA+ +DW AEE RR Sbjct: 61 MSAFERSKIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRT 120 Query: 121 YGRVVGARHPEVRQLVVREPVGVCAAISPWNFPFGQAMKKVAAAMAAGCTVVLKGPSESP 180 YGR+V AR P Q+V++EPVG AA +PWNFP Q ++K++AA+AAGC++++K P E+P Sbjct: 121 YGRLVPARVPGAYQMVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETP 180 Query: 181 AAIVLMAQLFHDAGVPAGVFNIVWGVSSEISQQLIASPLVRTISFTGSIPVGSQLAAQAA 240 A+ + + + DAGVPAGV N+V+GV +EIS+ LI P+++ ISFTGS PVG QLAA A Sbjct: 181 ASPAELIRAYADAGVPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALAG 240 Query: 241 LHMKRMSMELGGHAPVLVFDDAEVDAAARLLARLKIRNAGQVCVSPSRFFVHQRIYDRFV 300 HMKR +MELGGHAP +VFDDA+VD AA++LA K RNAGQVCVSP+RF V + +++ FV Sbjct: 241 QHMKRATMELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAFV 300 Query: 301 VALADALQQVKVGSGLEAGVQMGPLIHGRRFDAVHGLIEDAVSQGAQLVTGGRRIGERGH 360 +Q+KVG+GLEAG+ MGP+ + RR A+ LI+DAVSQGAQL TGG+RIG +G+ Sbjct: 301 DKFVTYAEQLKVGNGLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRIGNKGY 360 Query: 361 FHAPTLLADVPPTARVMQEEPFGPVAAVTPFAHTAQALAMANALPLGLASYVFTQSLGTA 420 F PT+L ++P AR M EEPFGP+A + F + + AN LP GLA+Y F +S T Sbjct: 361 FFQPTVLTNLPLQARAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHTK 420 Query: 421 HEVASGLQAGMVNINHSGMAHPELPFGGIGESGFGSEGGTESFDSFLVTKMISQIS 476 H +A+ +++GM+ INH G+A PELPFGGI +SG+GSEGGTE+ ++++ TK+++Q+ Sbjct: 421 HALAARVESGMLTINHLGLALPELPFGGIKDSGYGSEGGTEAIEAYINTKLVTQLD 476 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 379 bits (972), Expect = e-106, Method: Compositional matrix adjust. Identities = 198/468 (42%), Positives = 286/468 (61%) Query: 6 QLYINGRFHGLDGRRYQEVRDPATGDVLGRLPWAEAGDIEEAIQAAHAAFQQWRKVSPLE 65 Q ++NG++ D V +PA G++LG +P A + AI+AA+AA+ W+K + E Sbjct: 13 QAFVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANAAWPAWKKKTAKE 72 Query: 66 RSDILRRAAALTRERAQAMAPAITMDNGKPLADAVAEILNAAEHLDWHAEEGRRLYGRVV 125 RS ILRR L +A +T + GKPLA+A EI AA ++W AEEG+R YG + Sbjct: 73 RSAILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWFAEEGKRTYGDTI 132 Query: 126 GARHPEVRQLVVREPVGVCAAISPWNFPFGQAMKKVAAAMAAGCTVVLKGPSESPAAIVL 185 + P R +V++E +GVCAAI+PWNFP +K A+AAGC +VLK +P + + Sbjct: 133 PSPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFSALA 192 Query: 186 MAQLFHDAGVPAGVFNIVWGVSSEISQQLIASPLVRTISFTGSIPVGSQLAAQAALHMKR 245 +A L AG+PAGVF++V G I ++ ++P+VR ISFTGS VG L Q+A +K+ Sbjct: 193 LAVLAERAGIPAGVFSVVTGTPKGIGGEMTSNPIVRKISFTGSTGVGKLLMQQSASSIKK 252 Query: 246 MSMELGGHAPVLVFDDAEVDAAARLLARLKIRNAGQVCVSPSRFFVHQRIYDRFVVALAD 305 +S+ELGG+AP +VFDDA++DAA K RNAGQ CV +R +V +YD F L + Sbjct: 253 LSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQDGVYDAFAAKLVE 312 Query: 306 ALQQVKVGSGLEAGVQMGPLIHGRRFDAVHGLIEDAVSQGAQLVTGGRRIGERGHFHAPT 365 A+++ KVG G E GV GPLI+ + V + DAV++GA+++ GG+R F PT Sbjct: 313 AVKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGKRHALGHSFFEPT 372 Query: 366 LLADVPPTARVMQEEPFGPVAAVTPFAHTAQALAMANALPLGLASYVFTQSLGTAHEVAS 425 +LADV P +V +EE FGP+A + F + LA+AN GLASY +++ +G VA Sbjct: 373 VLADVTPAMQVAREETFGPMAPLFRFKTDEEVLALANDTEFGLASYFYSRDIGRIWRVAE 432 Query: 426 GLQAGMVNINHSGMAHPELPFGGIGESGFGSEGGTESFDSFLVTKMIS 473 GL++GMV IN +++ PFGG+ +SG G EG D +LV K + Sbjct: 433 GLESGMVGINTGLISNEVAPFGGVKQSGLGREGSHYGIDDYLVVKYLC 480 >gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 315 bits (807), Expect = 5e-87, Method: Compositional matrix adjust. Identities = 193/481 (40%), Positives = 280/481 (58%), Gaps = 8/481 (1%) Query: 1 MYPDLQLYINGRFHGLDGRRYQEVRDPATGDVLGRLPWAEAGDIEEAIQAAHAAFQQWRK 60 + P QL I + G R +V DPATG V +P A D A++AA AAF WR Sbjct: 11 LMPGAQL-IGADWRGAADGRQLDVSDPATGQVFASVPDGGAADARAAVEAAVAAFAAWRA 69 Query: 61 VSPLERSDILRRAAALTRERAQAMAPAITMDNGKPLADAVAEILNAAEHLDWHAEEGRRL 120 +R+ I++R L + I+ + GKPLA+A E+ AA +++W EE R Sbjct: 70 TPAKQRAGIIKRWNDLLLAHQDDLGRLISREQGKPLAEAKGEVAYAASYVEWFGEEATRA 129 Query: 121 YGRVVGARHPEVRQLVVREPVGVCAAISPWNFPFGQAMKKVAAAMAAGCTVVLKGPSESP 180 G ++ A R + ++EPVGV AAI+PWNFP +K+A A+AAGCTVV K ++P Sbjct: 130 NGDIIPAPVTGRRMMALKEPVGVVAAITPWNFPAAMIARKIAPALAAGCTVVCKPAEDTP 189 Query: 181 AAIVLMAQLFHDAGVPAGVFNIVWGV---SSEISQQLIASPLVRTISFTGSIPVGSQLAA 237 + + +L +AGVP GV NIV + E+ +A VR ISFTGS VG LA Sbjct: 190 LTSLALVRLAQEAGVPVGVINIVTASRERTPEVVDVWLADGRVRKISFTGSTAVGKHLAR 249 Query: 238 QAALHMKRMSMELGGHAPVLVFDDAEVDAAARLLARLKIRNAGQVCVSPSRFFVHQRIYD 297 +A +K++S+ELGG+AP +VFDDA+VDAA + K RN GQ CVSP+R +V +++YD Sbjct: 250 HSADTLKKLSLELGGNAPFIVFDDADVDAAIDGVMAAKFRNGGQTCVSPNRIYVQEKVYD 309 Query: 298 RFVVALADALQQVKVGSGLEAGVQMGPLIHGRRFDAVHGLIEDAVSQGAQLVTGGRRIGE 357 FV L + +KVG + Q+GP+I+ R + + DA+++GA+++TGG+R+ Sbjct: 310 AFVDKLGARVAALKVGPATDPASQIGPMINARAIAKIDQHVRDAIARGARVITGGKRLQG 369 Query: 358 RG----HFHAPTLLADVPPTARVMQEEPFGPVAAVTPFAHTAQALAMANALPLGLASYVF 413 G +++APT+LADV + EE FGPVA +T FA + +A ANA P GLA+Y + Sbjct: 370 PGFGSDNYYAPTVLADVTGAMQCSCEETFGPVAPITRFATEDEVIAAANATPFGLAAYFY 429 Query: 414 TQSLGTAHEVASGLQAGMVNINHSGMAHPELPFGGIGESGFGSEGGTESFDSFLVTKMIS 473 + + H V L++G+V +N +A PFGG+ ESG+G EG D +L TK + Sbjct: 430 STDVRRIHRVTDALESGIVGVNEGALAAEAAPFGGVKESGYGREGSVHGLDDYLHTKYVC 489 Query: 474 Q 474 Q Sbjct: 490 Q 490 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 280 bits (715), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 179/475 (37%), Positives = 261/475 (54%), Gaps = 18/475 (3%) Query: 6 QLYINGRFHGLDGRRYQEVRDPATGDVLGRLPWAEAGDIEEAIQAAHAAFQQWRKVSPLE 65 YI+G++ G V PAT + + + A D+++A+QAA A W SPLE Sbjct: 17 HFYIDGKWQPPQGSERAAVVAPATEERVCEIALGNAADVDQAVQAARRAIPAWAATSPLE 76 Query: 66 RSDILRRAAALTRERAQAMAPAITMDNGKPLADA-VAEILNAAEHLDWHAEEGRRLYGRV 124 R+ +L R AL ER++ A A+T++ G P++ A A + AAEHL R Sbjct: 77 RAALLGRVHALLLERSELFAQALTLEMGAPISYARTAHVPLAAEHLR---------VARD 127 Query: 125 VGARHPEVR----QLVVREPVGVCAAISPWNFPFGQAMKKVAAAMAAGCTVVLKGPSESP 180 A +P +R +VREP+GVCA I+PWN+P Q KV A+AAGCTVVLK SP Sbjct: 128 NLADYPFIRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPSELSP 187 Query: 181 AAIVLMAQLFHDAGVPAGVFNIVWGVSSEISQQLIASPLVRTISFTGSIPVGSQLAAQAA 240 + +L A++ H+AGVPAGVFN+V G +E+ L A P V IS TGS G +A AA Sbjct: 188 LSALLFAEIVHEAGVPAGVFNLVNGSGAEVGAALSAHPQVDMISITGSTRAGVLVAQAAA 247 Query: 241 LHMKRMSMELGGHAPVLVFDDAEVDAAARLLARLKIRNAGQVCVSPSRFFVHQRIYDRFV 300 + +KR++ ELGG +P LV DA+++ A +RN GQ C +P+R V + R Sbjct: 248 VTVKRVAQELGGKSPNLVLPDADLERAIPPGVAAALRNMGQSCSAPTRLIVPRAALARVH 307 Query: 301 VALADALQQVKVGSGLEAGVQMGPLIHGRRFDAVHGLIEDAVSQGAQLVTG--GRRIG-E 357 L Q+KVG +A GPL + +F V +IE + GA+L+ G GR G Sbjct: 308 ELALATLAQMKVGDPNDAATTHGPLANRAQFLRVQQMIEAGLQDGARLLAGGPGRPDGLA 367 Query: 358 RGHFHAPTLLADVPPTARVMQEEPFGPVAAVTPFAHTAQALAMANALPLGLASYVFTQSL 417 +G++ PT+ ++V + QEE FGPV A+ P+ +A+A+AN GL ++V + Sbjct: 368 QGYYARPTIFSEVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLGAHVQGRDK 427 Query: 418 GTAHEVASGLQAGMVNINHSGMAHPELPFGGIGESGFGSEGGTESFDSFLVTKMI 472 VA+ +Q+G V++N+ P+ PFGG +SG G E G E + ++ K I Sbjct: 428 DQVRAVAARIQSGQVHLNYPAW-DPQAPFGGYKQSGNGREYGVEGMEEYMEVKAI 481 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,427,907 Number of extensions: 59667 Number of successful extensions: 278 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 232 Number of HSP's successfully gapped: 21 Length of query: 480 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 391 Effective length of database: 1,198,455 Effective search space: 468595905 Effective search space used: 468595905 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)