BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0167.p (536 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313232|ref|YP_003777324.1| 3-(3-hydroxyphenyl) propionate ... 983 0.0 gi|300309878|ref|YP_003773970.1| 2-polyprenyl-6-methoxyphenol hy... 111 1e-25 gi|300310882|ref|YP_003774974.1| 2-polyprenyl-6-methoxyphenol hy... 87 3e-18 >gi|300313232|ref|YP_003777324.1| 3-(3-hydroxyphenyl) propionate hydroxylase [Herbaspirillum seropedicae SmR1] Length = 544 Score = 983 bits (2540), Expect = 0.0, Method: Compositional matrix adjust. Identities = 482/534 (90%), Positives = 496/534 (92%), Gaps = 2/534 (0%) Query: 2 STAVQAQVLIIGAGPVGVTLANLLGTYGISTLIIERNLDVLDYPRAVGIDDEALRTFQSA 61 + +QAQVLI+GAGPVGVTL NLLGTYGISTL+IERNLDVLDYPRAVGIDDEALRTFQSA Sbjct: 12 AQVMQAQVLIVGAGPVGVTLGNLLGTYGISTLLIERNLDVLDYPRAVGIDDEALRTFQSA 71 Query: 62 GMADAIVRDVIQNVPMRMYTADRRCFAEIQPTTREFGWYRRNLFSQPLGEATLRQGLQRF 121 GMADAIVRDVIQNVPMRMYTADRRCFAEIQPTTREFGWYRRNLFSQPLGEATLR GL+RF Sbjct: 72 GMADAIVRDVIQNVPMRMYTADRRCFAEIQPTTREFGWYRRNLFSQPLGEATLRLGLRRF 131 Query: 122 PHVELRSGVELLDLVQDEQGVLATISDAHGMQSYLAAQYAVGCDGGRSTVREKILKLPYE 181 HV LRSGVELLDLVQDE GV A+I D HG Q+ L AQY VGCDGGRSTVREKILKLPYE Sbjct: 132 EHVSLRSGVELLDLVQDEAGVQASIIDEHGAQATLRAQYVVGCDGGRSTVREKILKLPYE 191 Query: 182 GKTHPAKWVVIECDGDPLDAPYTALHCEPQRPYVCLALPYGVRRWEFMLFPGEDGEQMLA 241 GKTHPAKWVVIECDGDPLDAPYTALHCEPQRPYVCLALPYGVRRWEFMLFPGEDGEQMLA Sbjct: 192 GKTHPAKWVVIECDGDPLDAPYTALHCEPQRPYVCLALPYGVRRWEFMLFPGEDGEQMLA 251 Query: 242 PVKVRELLREHVADVDALNIIRARVYTHNSRVAGSLVCGRICLAGDAAHITPPWIGQGLN 301 P KVRELLREHV DVDALNIIRARVYTHNSRVAGSLVCGRICLAGDAAHITPPWIGQGLN Sbjct: 252 PEKVRELLREHVRDVDALNIIRARVYTHNSRVAGSLVCGRICLAGDAAHITPPWIGQGLN 311 Query: 302 AGLRDAFNLAWKLAWVLQGRMKPALLASYHDERHAHVKAMIDLADLFGAVLSQKNRLLAW 361 AGLRDAFNLAWKLAWVLQGRMKP LLASYH+ERHAHVKAMIDLADLFGAVLSQKNRLLAW Sbjct: 312 AGLRDAFNLAWKLAWVLQGRMKPELLASYHEERHAHVKAMIDLADLFGAVLSQKNRLLAW 371 Query: 362 CRDKFFLAIKDIPRVRDYVLQMKFKPMPSFHSQAVMQGDPRNRDDLVGRMFLQSLVENGE 421 CRDKFFLAIKDIPRVRDYVLQMKFKPMPSF SQAVMQ D RNR DLVGRMFLQSLVENG Sbjct: 372 CRDKFFLAIKDIPRVRDYVLQMKFKPMPSFSSQAVMQDDVRNRSDLVGRMFLQSLVENGA 431 Query: 422 GEVMRLDDLCGSRFTLLSWRCDVLPAATVDPDLRRRMQAFGCDYLQGVRSRSGARGVPLT 481 GEVMRLDDLCG RF LLSWRCDVL AA +P LR RMQAFGCDYLQGVRSRSG RG L Sbjct: 432 GEVMRLDDLCGGRFVLLSWRCDVLAAA--EPGLRSRMQAFGCDYLQGVRSRSGPRGQVLA 489 Query: 482 GSGAVIQDHENKLNAWFKEKGVDWVLLRPDRFIAAAGRADDAPAVMQLFLDRVA 535 G+G VIQD+ENKLNAWFKEKGVDWVLLRPDRFIAAAGRA DAP V+ +LD+VA Sbjct: 490 GAGVVIQDYENKLNAWFKEKGVDWVLLRPDRFIAAAGRAGDAPEVVARYLDQVA 543 >gi|300309878|ref|YP_003773970.1| 2-polyprenyl-6-methoxyphenol hydroxylase [Herbaspirillum seropedicae SmR1] Length = 589 Score = 111 bits (278), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 101/342 (29%), Positives = 161/342 (47%), Gaps = 30/342 (8%) Query: 9 VLIIGAGPVGVTLANLLGTYGISTLIIERNLDVLDYPRAVGIDDEALRTFQSAGMADAIV 68 V+IIGAGPVG+ A L GI+ ++++ + + RA+ L F G +V Sbjct: 33 VVIIGAGPVGLACAIDLARQGIAVVLVDDDDTLSTGSRAICFAKRTLDIFDRIGCGQRMV 92 Query: 69 RDVIQNVPMRMYTADRRCFA-EIQPTTREFGWYRRNLFS--QPLGEATLRQGLQRFPHVE 125 + +++ D+ + ++ P E G R + Q E L + Q++PH+E Sbjct: 93 DKGVGWNRGKVFLRDQAVYEFDLLP---ESGHARPAFINLQQYYVEGYLLEQAQQWPHIE 149 Query: 126 LRSGVELLDLVQDEQGVLATISDAHGMQSYLAAQYAVGCDGGRSTVREKILKLPYEGKTH 185 +R ++ + E T+ G + L A+Y V DG RS +R K++ L +G T Sbjct: 150 IRWKNKVSAIAHHEDHEALTVDTPQGSYT-LKARYVVAADGSRSPLR-KMMGLESQGHTF 207 Query: 186 PAKWVVIECDGDPLDAPYTALH-------CEPQRPYVCLALPYGVRRWEFMLFPGEDGEQ 238 ++++ + +DAP+ A PQ+ + P V R +F L G D + Sbjct: 208 RDRFLIADVK---MDAPFPAERWFWFDPPFHPQQSVLLHRQPDDVWRIDFQL--GWDADP 262 Query: 239 MLAPV------KVRELLREHVADVDALNIIRARVYTHNSRVAGSLVCGRICLAGDAAHIT 292 +L +VR LL E V+ ++ VYT + GR+ GDAAH Sbjct: 263 VLEKTPERVIPRVRALLGEEVS----FSLEWVSVYTFSCVRMERFRHGRVLFVGDAAHCV 318 Query: 293 PPWIGQGLNAGLRDAFNLAWKLAWVLQGRMKPALLASYHDER 334 P+ +G N+G++DA NLAWKLA VLQG+ +LL SY ER Sbjct: 319 SPFGARGANSGIQDAENLAWKLAHVLQGKAPDSLLDSYASER 360 >gi|300310882|ref|YP_003774974.1| 2-polyprenyl-6-methoxyphenol hydroxylase [Herbaspirillum seropedicae SmR1] Length = 547 Score = 87.0 bits (214), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 125/533 (23%), Positives = 209/533 (39%), Gaps = 64/533 (12%) Query: 9 VLIIGAGPVGVTLANLLGTYGISTLIIERNLDVLDYPRAVGIDDEALRTFQSAGMADAIV 68 V++IGAGP+G+T A G+ ++++ N V RAV L + G+A +V Sbjct: 28 VVVIGAGPIGLTAALECAARGLPVVVLDDNDTVSIGSRAVCYAKRPLEIWDRLGVAPRMV 87 Query: 69 RDVIQNVPMRMYTADRRCFA-EIQPTTREFGWYRRNLFSQPLGEATLRQGLQRFPHVELR 127 + +++ +R + ++ P NL Q E L + Q P ++LR Sbjct: 88 EKGVSWKLGKVFFEEREVYRFDLLPEHAHKMPAMINL-QQYYLEQYLVEACQAQPLIDLR 146 Query: 128 SGVELLDLVQDEQGVLATISDAHGMQSYLAAQYAVGCDGGRSTVREKILKLPYEGKTHPA 187 +++ L QD+ + G+ + A + + CDG S R K++ + G+ Sbjct: 147 WKHKMISLHQDDTHATLDVETPDGI-FRMEADWVIACDGANSDTR-KMVGAEFSGQFFQD 204 Query: 188 KW----VVIECDGDP-----LDAPY-----TALH--CEPQRPYVCLALPYGVRRWEFMLF 231 ++ V+++ D D P+ LH C+ V R +F L Sbjct: 205 RFLIADVIMKADFPAERWFWFDPPFHRGQSVLLHKQCD------------NVWRIDFQLG 252 Query: 232 PGEDGEQMLAPVKVRELLREHVADVDALNIIRARVYTHNSRVAGSLVCGRICLAGDAAHI 291 D E+ P KV ++ + + VY R + R+ AGDAAH Sbjct: 253 WEADPEEEKKPEKVIPRIQAMLGKEVEFELEWVSVYQFACRRIDKMRYQRVLFAGDAAHQ 312 Query: 292 TPPWIGQGLNAGLRDAFNLAWKLAWVLQGRMKPALLASYHDERHAHVKAMIDLADLFGAV 351 P+ +G N G++D NL WKL V+ G+ P+ + SYH+ER + + Sbjct: 313 VSPFGARGANTGVQDIDNLVWKLKLVIDGKAPPSFIDSYHEERAWAADDNLRQSTRSTDF 372 Query: 352 LSQKNRLLAWCRDKFFLAIKDIPRVRDYVLQMKFK-PMPSFHSQAVMQGDPRNRDDLV-- 408 ++ K+R RD + R V + P HS P N D Sbjct: 373 ITPKSRASKALRDAVLGLAETEEFARTLVNSGRLSTPTSYVHS-------PLNTADAALF 425 Query: 409 -GRMFLQSLVENG----EGEVMRLDDLCGSRFTLLSWRCDVLPAATVDPDLRRRMQAFGC 463 GRM ++ + EG+ L + G+ F +L + P A ++ Sbjct: 426 GGRMAPGTVCADAPVLREGQPGWLLESLGAGFVVLFFGASA-PQAVIE------------ 472 Query: 464 DYLQGVRSRSGARGVPLTGSGAVIQDHENKLNAWFKEKGVDWVLLRPDRFIAA 516 G+ +R G P GSGA + D E + L+RPD+ +AA Sbjct: 473 --AAGISARVVTVGAP--GSGAELIDREGLAALRYAASEGCVYLVRPDQHVAA 521 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.140 0.435 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,765,238 Number of extensions: 78695 Number of successful extensions: 327 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 325 Number of HSP's successfully gapped: 3 Length of query: 536 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 446 Effective length of database: 1,193,720 Effective search space: 532399120 Effective search space used: 532399120 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 133 (55.8 bits)