BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0154.p (484 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 911 0.0 gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrog... 317 1e-87 gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrog... 314 1e-86 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 310 2e-85 gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde deh... 303 2e-83 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 911 bits (2354), Expect = 0.0, Method: Compositional matrix adjust. Identities = 464/484 (95%), Positives = 475/484 (98%) Query: 1 MSLHLQHFIAGQASEASGGQRMNLVNPINEEVYASAAQGTAEDVARAVAAARAQLDGGAW 60 MSLHLQHFIAG+A+EAS GQRMNLVNPINEE+YASAAQGTA DVARAVAAA+AQL+GGAW Sbjct: 1 MSLHLQHFIAGEATEASDGQRMNLVNPINEEIYASAAQGTAADVARAVAAAKAQLEGGAW 60 Query: 61 SRLSGLQRGTLLHALAALVERDADLLADMDANAIGRSPMEPRMMDVPNAVSHLRAAAGWA 120 S+LSGLQRG LLH LAALVERD DLLADMDA AIGRSPMEPRMMDVPNA+SHLRAAAGWA Sbjct: 61 SKLSGLQRGQLLHKLAALVERDTDLLADMDARAIGRSPMEPRMMDVPNAISHLRAAAGWA 120 Query: 121 NQMEGRTIPTAGYMGKPTLSYTIREPIGVVGAILPWNAPLMITSWKVAALLAAGCTVVIK 180 NQMEGRTIPTAGYMGKPTLSYT+REPIGVVGAILPWNAPLMITSWKVAALLAAGCTVVIK Sbjct: 121 NQMEGRTIPTAGYMGKPTLSYTVREPIGVVGAILPWNAPLMITSWKVAALLAAGCTVVIK 180 Query: 181 PAEETPQSALHLARLAQEAGFPDGVINVVTGYGNEVGRALCEHPDVAKISFTGSPEAGRA 240 PAEETPQSALHLARLAQEAGFPDGVINVVTGYGN VGRALCEHPDVAKISFTGSPEAGRA Sbjct: 181 PAEETPQSALHLARLAQEAGFPDGVINVVTGYGNSVGRALCEHPDVAKISFTGSPEAGRA 240 Query: 241 IQRIAGEQFKRVTLELGGKSPQIVFDDAPFEDALFGCTLGLFVNQGQVCAAGSRILVQRS 300 IQRIAGEQFKRVTLELGGKSPQIVFDDAPFEDALFGCTLGLFVNQGQVCAAGSRILVQRS Sbjct: 241 IQRIAGEQFKRVTLELGGKSPQIVFDDAPFEDALFGCTLGLFVNQGQVCAAGSRILVQRS 300 Query: 301 LAQRFAKALAEAAAGITVGDPRQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGGVS 360 LAQRFAKALAEAAAGITVGDP QPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGGVS Sbjct: 301 LAQRFAKALAEAAAGITVGDPSQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGGVS 360 Query: 361 RPDKGWFVQPTIFADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDSRYGLAATVWT 420 RPDKGWFVQPTIFADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDS+YGLAATVWT Sbjct: 361 RPDKGWFVQPTIFADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDSKYGLAATVWT 420 Query: 421 GNLARAHRVAAGVKVGAIGVNCWSPLDANLPWGGIKDSGMGREGGLAGALAYTEEKTITV 480 NLARAHRVAAGVKVGA+GVNCWSPLDANLPWGGIKDSGMGREGGLAGALAYTEEKT+TV Sbjct: 421 TNLARAHRVAAGVKVGAVGVNCWSPLDANLPWGGIKDSGMGREGGLAGALAYTEEKTVTV 480 Query: 481 LLAS 484 LLAS Sbjct: 481 LLAS 484 >gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 317 bits (812), Expect = 1e-87, Method: Compositional matrix adjust. Identities = 193/480 (40%), Positives = 271/480 (56%), Gaps = 13/480 (2%) Query: 6 QHFIAGQASEASGGQRMNLVNPINEEVYASAAQGTAEDVARAVAAARAQLDGGAWSRLSG 65 QHFIA + + G+ + +++P + + + + A+G A D+ +AV AAR +G AW RLS Sbjct: 3 QHFIANRWVAPTSGESIPVLDPSDGQPFDAIARGNATDIDQAVRAARLAYEG-AWGRLSA 61 Query: 66 LQRGTLLHALAALVERDADLLADMDANAIGRSPMEPRMMDVPNAVSHLRAAAGWANQMEG 125 +R LL LA ++ + LA ++ G+ PM+ D V + AG A+++ G Sbjct: 62 AERSRLLMKLAYKLQDHQEELAQLEGRDCGK-PMKQARADAAAIVRYFEFYAGAADKLHG 120 Query: 126 RTIP-TAGYMGKPTLSYTIREPIGVVGAILPWNAPLMITSWKVAALLAAGCTVVIKPAEE 184 TIP AGY T+REP GV G I+PWN P+ I V LAAG T V+KPAE+ Sbjct: 121 DTIPYQAGYT-----VLTLREPHGVTGHIVPWNYPMQIFGRCVGGALAAGNTCVVKPAED 175 Query: 185 TPQSALHLARLAQEAGFPDGVINVVTGYGNEVGRALCEHPDVAKISFTGSPEAGRAIQRI 244 S L +A LA E GFP+G +N+VTGYG+E G L +HPDV ISFTGSP G+ + R Sbjct: 176 ACLSILRVAELAAEVGFPEGALNIVTGYGHEAGDLLVKHPDVNHISFTGSPNVGKLVVRA 235 Query: 245 AGEQFKRVTLELGGKSPQIVFDDAPFEDALFGCTLGLFVNQGQVCAAGSRILVQRSLAQR 304 A E VTLELGGKSPQIVF+DA E L + N GQ C+AGSR+LVQR ++ Sbjct: 236 AAEHHTPVTLELGGKSPQIVFEDADMEAMLPVVVNAIVQNAGQTCSAGSRLLVQRGAYEQ 295 Query: 305 FAKALAEAAAGITVGDPRQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGG---VSR 361 + L+ + + VG Q + GP+ +K Q ERV ++ +G L A G Sbjct: 296 VLERLSGLFSQLQVGS-SQMDLDCGPLIRKTQQERVAGFLSDAQRDGIALAAQGKIAAGV 354 Query: 362 PDKGWFVQPTIFADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDSRYGLAATVWTG 421 P G++ P + D +A+EE+FGPV + F+ E AIALAN + YGL A+VWT Sbjct: 355 PTGGFYQAPALLRDVPPRHRLAQEEVFGPVLAAMPFEDEAHAIALANGTDYGLVASVWTR 414 Query: 422 NLARAHRVAAGVKVGAIGVNCW-SPLDANLPWGGIKDSGMGREGGLAGALAYTEEKTITV 480 + R R+A ++ G + +N + + LP+GG+K SG GRE G +T KTI + Sbjct: 415 DGGRQMRLARAIRSGQVFINNYGAGGGVELPFGGVKASGHGREKGFEALYGFTVLKTIAI 474 >gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 504 Score = 314 bits (805), Expect = 1e-86, Method: Compositional matrix adjust. Identities = 171/421 (40%), Positives = 240/421 (57%), Gaps = 5/421 (1%) Query: 60 WSRLSGLQRGTLLHALAALVERDADLLADMDANAIGRSPMEPRMMDVPNAVSHLRAAAGW 119 W LS RG L+A+ A++ +A+ LA ++A + G+ P+ ++ AGW Sbjct: 75 WWALSHAARGRALYAVGAVIRAEAEPLARLEAISSGK-PIRDCRAEMQKVAEMFEYYAGW 133 Query: 120 ANQMEGRTIPTAGYMGKPTLSYTIREPIGVVGAILPWNAPLMITSWKVAALLAAGCTVVI 179 A++ G IP L+YT REP G V + PWNAP W++ LA G V++ Sbjct: 134 ADKFYGEVIPVP----SSHLNYTRREPYGTVLQMTPWNAPAFTCGWQLGPALATGNAVLL 189 Query: 180 KPAEETPQSALHLARLAQEAGFPDGVINVVTGYGNEVGRALCEHPDVAKISFTGSPEAGR 239 KP+E TP S+L +ARL ++AG P G++NV+ G G + V K+ F GSP G Sbjct: 190 KPSELTPFSSLAIARLGEQAGLPAGLVNVLAGLGQTMVPQAMATWTVKKVIFVGSPATGA 249 Query: 240 AIQRIAGEQFKRVTLELGGKSPQIVFDDAPFEDALFGCTLGLFVNQGQVCAAGSRILVQR 299 I + A + LELGGKS I+F+DA A FG +F GQ C AGSR+LVQR Sbjct: 250 LIAKAAAARVMPCVLELGGKSANIIFEDADLRLAAFGAQAAIFSGAGQSCVAGSRLLVQR 309 Query: 300 SLAQRFAKALAEAAAGITVGDPRQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGGV 359 + RF + +A A I +G P +GP+ + Q+E + R + +GL+ GATL AG Sbjct: 310 KVYDRFVETVAAGAEKIRLGAPLDDSTEVGPINNRKQYEHIQRMVARGLEAGATLAAGHT 369 Query: 360 SRPDKGWFVQPTIFADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDSRYGLAATVW 419 ++G+FV+PT+ A A+N M +AR EIFGPV I F+ EEEAIA+ANDS +GLA VW Sbjct: 370 RYGEEGYFVRPTLLAHASNAMEVARSEIFGPVAVAIPFEDEEEAIAIANDSEFGLAGAVW 429 Query: 420 TGNLARAHRVAAGVKVGAIGVNCWSPLDANLPWGGIKDSGMGREGGLAGALAYTEEKTIT 479 T ++ARAHRVAA V G VN + ++ P+GG SG GR G+ YT+ K++ Sbjct: 430 TRDVARAHRVAASVNAGTFWVNSYKTINVASPFGGFNRSGYGRSSGMEALYDYTQTKSVW 489 Query: 480 V 480 V Sbjct: 490 V 490 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 310 bits (793), Expect = 2e-85, Method: Compositional matrix adjust. Identities = 198/478 (41%), Positives = 259/478 (54%), Gaps = 15/478 (3%) Query: 5 LQHF-IAGQASEASGGQRMNLVNPINEEVYASAAQGTAEDVARAVAAARAQLDGGAWSRL 63 L HF I G+ G +R +V P EE A G A DV +AV AAR + AW+ Sbjct: 15 LDHFYIDGKWQPPQGSERAAVVAPATEERVCEIALGNAADVDQAVQAARRAIP--AWAAT 72 Query: 64 SGLQRGTLLHALAALVERDADLLADMDANAIGRSPMEPRMMDVPNAVSHLRAAAGWANQM 123 S L+R LL + AL+ ++L A +G R VP A HLR A Sbjct: 73 SPLERAALLGRVHALLLERSELFAQALTLEMGAPISYARTAHVPLAAEHLRVARD----- 127 Query: 124 EGRTIPTAGYMGKPTLSYTIREPIGVVGAILPWNAPLMITSWKVAALLAAGCTVVIKPAE 183 + ++ + +REPIGV I PWN P+ + KV LAAGCTVV+KP+E Sbjct: 128 ---NLADYPFIRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPSE 184 Query: 184 ETPQSALHLARLAQEAGFPDGVINVVTGYGNEVGRALCEHPDVAKISFTGSPEAGRAIQR 243 +P SAL A + EAG P GV N+V G G EVG AL HP V IS TGS AG + + Sbjct: 185 LSPLSALLFAEIVHEAGVPAGVFNLVNGSGAEVGAALSAHPQVDMISITGSTRAGVLVAQ 244 Query: 244 IAGEQFKRVTLELGGKSPQIVFDDAPFEDALFGCTLGLFVNQGQVCAAGSRILVQRSLAQ 303 A KRV ELGGKSP +V DA E A+ N GQ C+A +R++V R+ Sbjct: 245 AAAVTVKRVAQELGGKSPNLVLPDADLERAIPPGVAAALRNMGQSCSAPTRLIVPRAALA 304 Query: 304 RFAKALAEAAAGITVGDPRQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGGVSRPD 363 R + A + VGDP GP+A +AQF RV + I+ GL +GA LLAGG RPD Sbjct: 305 RVHELALATLAQMKVGDPNDAATTHGPLANRAQFLRVQQMIEAGLQDGARLLAGGPGRPD 364 Query: 364 ---KGWFVQPTIFADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDSRYGLAATVWT 420 +G++ +PTIF++ + M IA+EEIFGPV I+ +DT EEAIA+AND+ YGL A V Sbjct: 365 GLAQGYYARPTIFSEVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLGAHVQG 424 Query: 421 GNLARAHRVAAGVKVGAIGVNCWSPLDANLPWGGIKDSGMGREGGLAGALAYTEEKTI 478 + + VAA ++ G + +N + D P+GG K SG GRE G+ G Y E K I Sbjct: 425 RDKDQVRAVAARIQSGQVHLN-YPAWDPQAPFGGYKQSGNGREYGVEGMEEYMEVKAI 481 >gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 303 bits (777), Expect = 2e-83, Method: Compositional matrix adjust. Identities = 183/486 (37%), Positives = 265/486 (54%), Gaps = 22/486 (4%) Query: 7 HFIAGQASEASGGQRMNLVNPINEEVYASAAQGTAEDVARAVAAARAQLDGGAWSRLSGL 66 +FI G+ G+ ++P+ + A+ +AEDV A+ AA A +W + S Sbjct: 21 NFIGGKFVPPVKGEYFENISPVIGRAFCEVARSSAEDVELALDAAHAA--KKSWGKTSPT 78 Query: 67 QRGTLLHALAALVERDADLLADMDANAIGRSPMEPRMMDVPNAVSHLRAAAGWANQMEGR 126 +R +L +A +E + +LLA + G+ E D+P A+ H R A EG Sbjct: 79 ERANMLLKIADRMEANLELLATAETLDNGKPIRETMAADIPLAIDHFRYFAAAVRTQEGS 138 Query: 127 TIPTAGYMGKPTLSYTIREPIGVVGAILPWNAPLMITSWKVAALLAAGCTVVIKPAEETP 186 P + T +Y EP+GVVG I+PWN P+++ WK+A LAAG VV+KPAE+TP Sbjct: 139 ICP----IDNDTYAYHFHEPLGVVGQIIPWNFPILMAVWKLAPALAAGNCVVLKPAEQTP 194 Query: 187 QSALHLARLAQEAGFPDGVINVVTGYGNEVGRALCEHPDVAKISFTGSPEAGRAIQRIAG 246 S + L L + P GV+N+V G+G E G+ L + +AKI+FTG GR I + A Sbjct: 195 ASIMVLIELIADL-IPPGVVNIVQGFGVEAGKPLASNKRIAKIAFTGETTTGRLIMQYAS 253 Query: 247 EQFKRVTLELGGKSPQIVFDDA------PFEDALFGCTLGLFVNQGQVCAAGSRILVQRS 300 + VTLELGGKSP I F D F+ AL G + +NQG+VC SR+LVQ S Sbjct: 254 QNLIPVTLELGGKSPNIFFADVLDKDDDFFDKALEGFAM-FALNQGEVCTCPSRVLVQES 312 Query: 301 LAQRFAKALAEAAAGITVGDPRQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGGVS 360 + +RF + + A I G+P +G A + Q E++ YI G EGA +LAGG Sbjct: 313 IYERFIERALKRVAAIKQGNPLDKSTMIGAQASQEQLEKILSYIDIGKQEGAKVLAGG-G 371 Query: 361 RPD------KGWFVQPTIFADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDSRYGL 414 R + G++V+PT+F NN M I +EEIFGPV ++ +F EEEA+A+AND+ YGL Sbjct: 372 REELGGDLASGYYVKPTVF-QGNNKMRIFQEEIFGPVVSVTTFKDEEEALAIANDTLYGL 430 Query: 415 AATVWTGNLARAHRVAAGVKVGAIGVNCWSPLDANLPWGGIKDSGMGREGGLAGALAYTE 474 A +WT + RA R+ ++ G + NC+ A+ +GG K SG+GRE Y + Sbjct: 431 GAGLWTRDGTRAFRMGREIQAGRVWTNCYHLYPAHAAFGGYKQSGIGRENHKMMLDHYQQ 490 Query: 475 EKTITV 480 K + V Sbjct: 491 TKNLLV 496 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,551,214 Number of extensions: 66782 Number of successful extensions: 313 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 262 Number of HSP's successfully gapped: 21 Length of query: 484 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 395 Effective length of database: 1,198,455 Effective search space: 473389725 Effective search space used: 473389725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)