BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0153.p (485 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313243|ref|YP_003777335.1| amino acid permease family prot... 833 0.0 gi|300310613|ref|YP_003774705.1| amino-acid transporter transmem... 234 9e-63 >gi|300313243|ref|YP_003777335.1| amino acid permease family protein [Herbaspirillum seropedicae SmR1] Length = 485 Score = 833 bits (2153), Expect = 0.0, Method: Compositional matrix adjust. Identities = 451/485 (92%), Positives = 471/485 (97%) Query: 1 MSQLDCSKHDKEENALRKNALGVGFIIFFVVSAASPLSVIAGGFPIGIMLGNGPGTPALL 60 MSQ+DC+ DKEENALRKNALGVGFIIFFVVSAASPLSVIAGGFPIGIMLGNG GTPALL Sbjct: 1 MSQVDCTTQDKEENALRKNALGVGFIIFFVVSAASPLSVIAGGFPIGIMLGNGAGTPALL 60 Query: 61 VLILLLLLAFSVGYTTMARHVTNAGGFYAFSSAGLGGMAGGVAGVLAMFAYNILQIGLYG 120 VLILLLLLAFSVGYTTMARHVTNAGGFYAFSSAGLGG+AGGVAGVLAMFAYNILQIGLYG Sbjct: 61 VLILLLLLAFSVGYTTMARHVTNAGGFYAFSSAGLGGIAGGVAGVLAMFAYNILQIGLYG 120 Query: 121 MFGGVMSATIFDIFGVQLAWWVYAALAMLSVAVLGYRNIDLSARLLSVVVVAEYLAVLVL 180 MFGGVMSAT DIFG+ LAWWVYAA+AMLSVA+LGYRNIDLSARLLSVVVVAEYLAVLVL Sbjct: 121 MFGGVMSATFSDIFGIHLAWWVYAAVAMLSVAILGYRNIDLSARLLSVVVVAEYLAVLVL 180 Query: 181 DFCILRAGGDHGVNLDSFAPANVMSGTPSIGLLFAFAAFIGFEATTIYGEEAKNPKRAIP 240 DFCILR+GGD GVNLDSF+PANVMSGTPSIGLLFAFAAFIGFEATTIYGEEAKNPKRAIP Sbjct: 181 DFCILRSGGDRGVNLDSFSPANVMSGTPSIGLLFAFAAFIGFEATTIYGEEAKNPKRAIP 240 Query: 241 IATYTSVLLIGAFYGISLWALIIGAGSDKVVKLLEHMQDPTAFIYVMSDHYAGGGLTAVI 300 IATYTSVLLIG FYGISLW+LIIGAGSDKVVKLLE MQDPTAFIY+MSDHYAG GLTAVI Sbjct: 241 IATYTSVLLIGGFYGISLWSLIIGAGSDKVVKLLEGMQDPTAFIYLMSDHYAGPGLTAVI 300 Query: 301 RILFVISIYAGLLAFHSSAARYFYAVGRDGLLHRLLGTTHKTYQSPHMGSALQSAIAAVV 360 RILFVISIYAGLLAFHSSAARYFYAVGRDGLLHRLLGTTH+TYQSPHMGSALQSAIAAVV Sbjct: 301 RILFVISIYAGLLAFHSSAARYFYAVGRDGLLHRLLGTTHRTYQSPHMGSALQSAIAAVV 360 Query: 361 VTLFAVSGAHPILHLFAWFSSLAALCLMLLMIMTSAAVICYFRRHPQACDSAWRVRILPA 420 VTLFAV AHPILHLFAWFSSLAALCLMLLMIMTSAAVICYFRR P+AC+SAWRVRILPA Sbjct: 361 VTLFAVFDAHPILHLFAWFSSLAALCLMLLMIMTSAAVICYFRRRPEACNSAWRVRILPA 420 Query: 421 VSLVALLYILCTAILHFDVLTGASKSMSWALCSMIPLAILIGVMLASRLRRCAPMRFAAL 480 VSL+ALLYIL TAILHFDVLTGAS+SMS+ALCSMIPLAI++GVMLA+RLRRCAP+RFAAL Sbjct: 421 VSLLALLYILSTAILHFDVLTGASRSMSYALCSMIPLAIVLGVMLATRLRRCAPLRFAAL 480 Query: 481 GSHKL 485 GSHKL Sbjct: 481 GSHKL 485 >gi|300310613|ref|YP_003774705.1| amino-acid transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 234 bits (598), Expect = 9e-63, Method: Compositional matrix adjust. Identities = 152/478 (31%), Positives = 253/478 (52%), Gaps = 6/478 (1%) Query: 7 SKHDKEENALRKNALGVGFIIFFVVSAASPLSVIAGGFPIGIMLGNGPGTPALLVLILLL 66 ++ + L+K+A+G I V++ PL+ + GG P+ I LGNG G P V++ L Sbjct: 5 ARSTPDHTGLKKDAIGWFGIFLMVITTNGPLTSMGGGVPVSITLGNGIGLPGSYVVVCLA 64 Query: 67 LLAFSVGYTTMARHVTNAGGFYAFSSAGLGGMAGGVAGVLAMFAYNILQIGLYGMFGGVM 126 L F++G+ M +V N G FYA+ + GLG +AG + +A+ +Y + + Y M G M Sbjct: 65 YLLFAIGFCAMTPYVENGGAFYAYITKGLGSVAGVGSACIAVLSYISILLACYAMLGHFM 124 Query: 127 SATIFDIFGVQLAWWVYAALAMLSVAVLGYRNIDLSARLLSVVVVAEYLAVLVLDFCILR 186 + G+ WW++A + + +L R I+ S RLL ++++ E A+L D L Sbjct: 125 Q-VMMAAMGIAAPWWIWALVCAVLAHLLCVRGIEFSGRLLLLLMLCEVGAILGADVLELL 183 Query: 187 AGGDHG--VNLDSFAPANVMSGTPSIGLLFAFAAFIGFEATTIYGEEAKNPKRAIPIATY 244 G V+ F P +V + L+F A+F+GFE IY +EA++P+R++P A Y Sbjct: 184 RQHFTGAVVSFAPFWPQSVFTAGFGPSLIFVVASFMGFETAAIYAQEARDPQRSVPRAMY 243 Query: 245 TSVLLIGAFYGISLWALIIGAGSDKVVKLLEHMQDPTAFIYVMSDHYAGGGLTAVIRILF 304 S++ IG Y S++ ++ G D V++ + DP M AG + +L Sbjct: 244 FSIVFIGVLYAASIFLMLNHFGVDHAVQVAAN--DPGGMWLSMFQTLAGPTAARIGAVLV 301 Query: 305 VISIYAGLLAFHSSAARYFYAVGRDGLLHRLLGTTHKTYQSPHMGSALQSAIAAVVVTLF 364 + S++A +L+F + ARY++A+ GL+ +L TH ++SP++ SA+ I+ ++ + Sbjct: 302 ITSLFAAVLSFTNVVARYWHALSMRGLVWTMLSRTHPRHRSPYVASAITMCISCALILMC 361 Query: 365 AVSGAHPILHLFAWFSSLAALCLMLLMIMTSAAVICYFRRHPQACDSAWRVRILPAVSLV 424 A++G P+L W S +A+ ++ ++ + AV +FRRH Q+ SAWR + P S V Sbjct: 362 AMAGLDPMLQALPWASVPSAIGVLTCQVLAALAVASFFRRH-QSSVSAWRGLVAPVCSAV 420 Query: 425 ALLYILCTAILHFDVLTGASKSMSWALCSMIPLAILIGVMLASRLRRCAPMRFAALGS 482 LLY L I H +L+G S+ L I +A + A RLRR +P FA L Sbjct: 421 VLLYFLWQMIAHVQLLSGLPSVPSYLLAVSILIAGGLASFYACRLRRSSPDVFARLSE 478 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.141 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,329,345 Number of extensions: 49861 Number of successful extensions: 246 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 241 Number of HSP's successfully gapped: 3 Length of query: 485 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 396 Effective length of database: 1,198,455 Effective search space: 474588180 Effective search space used: 474588180 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)