BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0147.p (407 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310634|ref|YP_003774726.1| methyl-accepting chemotaxis dom... 539 e-154 gi|300309958|ref|YP_003774050.1| methyl-accepting chemotaxis tra... 206 3e-54 gi|300311753|ref|YP_003775845.1| methyl-accepting chemotaxis tra... 196 2e-51 gi|300311614|ref|YP_003775706.1| methyl-accepting chemotaxis tra... 98 1e-21 gi|300313024|ref|YP_003777116.1| methyl-accepting chemotaxis pro... 97 3e-21 >gi|300310634|ref|YP_003774726.1| methyl-accepting chemotaxis domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 568 Score = 539 bits (1388), Expect = e-154, Method: Compositional matrix adjust. Identities = 258/353 (73%), Positives = 298/353 (84%) Query: 1 MNVSFIGRFTIRQIFILLTGVISALMLAIALVALALERSDATLNAANESRYASFQLATEL 60 M +S + RF+IRQIFI+LT VI+ LM I L ALA++R DA LNAANESRYASF LATEL Sbjct: 1 MKISLVSRFSIRQIFIMLTAVITVLMTLIVLAALAVDRYDARLNAANESRYASFLLATEL 60 Query: 61 RQSSDDLTRLARTYVITADPAYEKQYQAILDIRDGKKPRPENYGRIYWDFVAADGKPPRP 120 RQSSDDLTRLARTYV+T DP+YE+QY+AILDIR+GKK RPE+YGRIYWDFVAADGK PRP Sbjct: 61 RQSSDDLTRLARTYVVTGDPSYEQQYEAILDIRNGKKARPEHYGRIYWDFVAADGKAPRP 120 Query: 121 DSSRSVALIALMREAGFTDAELAKLSEAKANSDALVKTETMAMNMVKGLYEDAQGNFTRR 180 DS++ VAL LMR AGFT AE+ KL EAK NSDALVKTE +AMNMVKGLY DAQGNFT+R Sbjct: 121 DSNQHVALTDLMRAAGFTAAEMGKLEEAKNNSDALVKTEVIAMNMVKGLYADAQGNFTKR 180 Query: 181 GEPDLVRARELMHDHAYHVNKARIMRPVDQFFAMLDARTLAQVEDARAHSATMALIFYAL 240 +PDL +ARELMH+HAYHV KA+IMRPVD+FFA+LDART QVE ARA + MA +FY+L Sbjct: 181 ADPDLAQARELMHNHAYHVYKAKIMRPVDEFFALLDARTTEQVESARAQATHMATLFYSL 240 Query: 241 MGVAVVVLACTLMLVYKAIRDPLAHAARVARRVAQGDLTAEISHHVRGETGELLEALRSM 300 + +A+VVLACTLMLVY AIR+PLAHAA VARRVA GDLTA+I ++GETGELLEAL M Sbjct: 241 IAIALVVLACTLMLVYHAIRNPLAHAASVARRVAHGDLTAQIDPAIKGETGELLEALTIM 300 Query: 301 QTGLQIIVTDVRDGAHRIDNSATEIAAGTLDLSTRTEQQASSLEETLDGRDHL 353 Q+GLQ IV DVR+GA I+ SA EIA+GTLDLS+RTEQQASSLEET + L Sbjct: 301 QSGLQTIVLDVRNGAQYINTSANEIASGTLDLSSRTEQQASSLEETASSMEEL 353 >gi|300309958|ref|YP_003774050.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 595 Score = 206 bits (524), Expect = 3e-54, Method: Compositional matrix adjust. Identities = 129/350 (36%), Positives = 194/350 (55%), Gaps = 6/350 (1%) Query: 1 MNVSFIGRFTIRQIFILLTGVISALMLAIALVALALERSDATLNAANESRYASFQLATEL 60 M + R +I+Q+FI L V+S L+ + + AL+ ++ L A++SRY S LA EL Sbjct: 1 MRETMTHRMSIKQLFIWLALVLSILVAVVVSLTGALKDANLELERAHQSRYDSTALANEL 60 Query: 61 RQSSDDLTRLARTYVITADPAYEKQYQAILDIRDGKKPRPENYGRIYWDFVAADGKPPRP 120 R++++D+T+ AR YV T DP E +Y I+DIR+GK+ RP NY R WD V A P Sbjct: 61 RRTAEDMTKFARAYVTTGDPNDEDRYYMIMDIRNGKRARPSNYDRFNWDLVNARKLALDP 120 Query: 121 DSSRSVALIALMREAGFTDAELAKLSEAKANSDALVKTETMAMNMVKGLYEDAQGNFTRR 180 + V L LM+ AGFTD ELAK+ EA D L K E A + VKG ++D +G +T Sbjct: 121 GAP-GVPLGELMKRAGFTDEELAKMQEALKLGDQLAKIEITAFHAVKGEFDDGEGKYTVI 179 Query: 181 GEPDLVRARELMHDHAYHVNKARIMRPVDQFFAMLDARTLAQVEDARAHSATMALIFYAL 240 G P+ A+EL+ D Y ++M PV+ F ++D RT A V A A + + L Sbjct: 180 GAPNQAMAQELVFDQTYRTLFGKLMSPVNDFLRLVDERTQANVRQAGKDYAELEQKIFWL 239 Query: 241 MGVAVVVLACTLMLVYKAIRDPLAHAAR----VARRVAQGDLTAEISHHVRGETGELLEA 296 + +VVVL L L Y+ IR + R V RRVA+GDLT + +G+ +L + Sbjct: 240 LAASVVVLFACLWLSYRIIRSQIGGEPRDAMSVLRRVAEGDLTVVVPLP-KGDKLSVLYS 298 Query: 297 LRSMQTGLQIIVTDVRDGAHRIDNSATEIAAGTLDLSTRTEQQASSLEET 346 + M ++ DV A + +++ +I++ + LS QQA+++EET Sbjct: 299 TQQMINKWTDVIGDVSGTATSLASASEQISSSSQALSHNAAQQAANVEET 348 >gi|300311753|ref|YP_003775845.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 559 Score = 196 bits (499), Expect = 2e-51, Method: Compositional matrix adjust. Identities = 126/336 (37%), Positives = 200/336 (59%), Gaps = 12/336 (3%) Query: 3 VSFIGRFTIRQIFILLTGVISALMLAIALVALALERSDATLNAANESRYASFQLATELRQ 62 +S + RF++RQ L +++ L++ + ER+ L A E+R+ + LA E RQ Sbjct: 1 MSILHRFSVRQALRGLVALVAVLVVMWGTATVYAERARQALAQAQETRFTALALAAEFRQ 60 Query: 63 SSDDLTRLARTYVITADPAYEKQYQAILDIRDGKKPRPENYGRIYWDFVAADGKPPRPDS 122 DDL +LAR+YV++ +P +Q+QA+LDIRDG++ RP+ YGR+YWD V ADG+PPRPDS Sbjct: 61 GVDDLAQLARSYVVSGEPRLLEQHQAVLDIRDGQRARPQQYGRVYWDLVGADGQPPRPDS 120 Query: 123 SRSVALIALMREAGFTDAELAKLSEAKANSDALVKTETMAMNMVKGLYEDAQGNFTRRGE 182 + +V+L LM +AG++ AELA+L EA SDAL TE AM ++ QG+ RG Sbjct: 121 TVAVSLRKLMEQAGYSPAELARLDEAWLMSDALASTERRAM-------QEMQGDAGARGP 173 Query: 183 PDLVRARELMHDHAYHVNKARIMRPVDQFFAMLDARTLAQVEDARAHSATMALIFYALMG 242 A++++H Y +++ I++ D +L+ RT +V A+ + +A + L Sbjct: 174 -----AQQMLHGDGYQLHRTSILQAFDDMVGLLEQRTAREVALAQLLAQRLAWLSTLLAV 228 Query: 243 VAVVVLACTLMLVYKAIRDPLAHAARVARRVAQGDLTAEISHHVRGETGELLEALRSMQT 302 VV+LA +L L+Y+ PLA A + ++A+GDL+ RG+ LL+AL+ ++ Sbjct: 229 AVVVILAVSLRLLYRGSAGPLAQATSMLAQLARGDLSDSAPVRARGQVAALLDALQRLRK 288 Query: 303 GLQIIVTDVRDGAHRIDNSATEIAAGTLDLSTRTEQ 338 + +V V GA I N+A ++AAGT + RTE+ Sbjct: 289 AIVRLVKGVHAGAAAIQNAAGDMAAGTEHVVARTEE 324 >gi|300311614|ref|YP_003775706.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 581 Score = 97.8 bits (242), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 66/163 (40%), Positives = 85/163 (52%), Gaps = 14/163 (8%) Query: 217 ARTLAQVEDARAHSATMALIFYALMGVAVVVLACTL----------MLVYKAIRDPLAHA 266 AR A +DA+ + + M + V LAC++ L+ ++I PL A Sbjct: 166 ARAFADSQDAQLQAEGAKAVADGAMAIEVT-LACSMAAVLAAIILGYLMARSIVKPLTEA 224 Query: 267 ARVARRVAQGDLTAEISHHVRGETGELLEALRSMQTGLQIIVTDVRDGAHRIDNSATEIA 326 RVA VA GDLT I H R ETG+L++ALR M L IV+ VR G I ++ EIA Sbjct: 225 VRVAENVAAGDLTTRIEPHSRDETGQLMQALRKMNDNLIEIVSGVRRGTESITTASGEIA 284 Query: 327 AGTLDLSTRTEQQASSLEETLDGRDHLH---RQAECQQRPAGQ 366 +G LDLS RTEQQA SLEET + + RQ R A Q Sbjct: 285 SGNLDLSARTEQQAGSLEETASAMEEMTSTVRQNADNARQANQ 327 >gi|300313024|ref|YP_003777116.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 571 Score = 96.7 bits (239), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 62/139 (44%), Positives = 81/139 (58%), Gaps = 5/139 (3%) Query: 231 ATMALIFYALMGVAVVVLACTLMLVYKAIRDPLAHAARVARRVAQGDLTAEISHHVRGET 290 A MA++ AL+ VV+ + + + PLA+A +AR+VA GDLTA+I R ET Sbjct: 189 ANMAIL--ALVTTCVVLAMALSWWIARLVARPLANAVDIARQVAAGDLTADIPTAHRDET 246 Query: 291 GELLEALRSMQTGLQIIVTDVRDGAHRIDNSATEIAAGTLDLSTRTEQQASSLEETLDGR 350 G+LLEALR M L IV +VR G I ++ EIA G +DLS+RTEQQA +LEET Sbjct: 247 GQLLEALRQMNGNLLNIVREVRQGTDTITTASAEIATGNMDLSSRTEQQAGALEETASAM 306 Query: 351 DHLH---RQAECQQRPAGQ 366 + L RQ R A Q Sbjct: 307 EELTSTVRQNSDNARQANQ 325 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.133 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,022,478 Number of extensions: 37456 Number of successful extensions: 246 Number of sequences better than 1.0e-08: 60 Number of HSP's gapped: 233 Number of HSP's successfully gapped: 60 Length of query: 407 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 320 Effective length of database: 1,207,925 Effective search space: 386536000 Effective search space used: 386536000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)