BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0146.p (387 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313247|ref|YP_003777339.1| branched-chain amino acid ABC t... 411 e-116 gi|300310948|ref|YP_003775040.1| branched-chain amino acid ABC t... 161 1e-40 gi|300312761|ref|YP_003776853.1| branched-chain amino acid ABC t... 108 8e-25 gi|300309516|ref|YP_003773608.1| branched-chain amino acid ABC t... 106 3e-24 gi|300311113|ref|YP_003775205.1| branched-chain amino acid ABC t... 74 2e-14 >gi|300313247|ref|YP_003777339.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 385 Score = 411 bits (1056), Expect = e-116, Method: Compositional matrix adjust. Identities = 204/359 (56%), Positives = 266/359 (74%), Gaps = 3/359 (0%) Query: 23 MQPAQAEEVRQVTLGLSAWLTQPIVQSAWHGAELAVEDANTLAARKKSSWRFRLLAQDDQ 82 MQ A+E + + LG + L++PI QSA GA+LA+++AN LAA+++S RF LL QDD Sbjct: 20 MQAGWAQERKTIALGFTGRLSEPIAQSALQGAQLAIDEANALAAKRRSPLRFELLPQDDH 79 Query: 83 GSANFGVNVARYFIKEKVAGVIGPWSSDAAMATAELYEAARIPQIGFTAGTSQWTSQGNR 142 G+ NF VN+ARY++KEKVAGVIGPWSSDAA+ATA +YE+A+IPQIGFTA +SQWT GNR Sbjct: 80 GNENFAVNIARYYVKEKVAGVIGPWSSDAALATAAIYESAQIPQIGFTATSSQWTGMGNR 139 Query: 143 MPFRVVGGTAEVGLTMAEVITTDLKGKRVFVVHNDSAYSNALTDELTARLVNRPGLTA-T 201 MPFRVVGGT E+ T+AEV T L+G+R+FV+ +D+AYS AL D L L A Sbjct: 140 MPFRVVGGTRELAATLAEVATRSLQGRRIFVIQSDAAYSRALADTLVENLGGSAQQIALA 199 Query: 202 RYLVGRRTTDFNATIKAANQYQADVIVFLALFPQAAAFVEEVRRSGSEAKVLLVGGASSL 261 RY VGRR+TDF+A +KAA +QADVIVF+ L+PQA AF++ R+ + A++LL GGAS+L Sbjct: 200 RYQVGRRSTDFHAALKAAEAHQADVIVFVGLYPQARAFIDGANRTRTTARLLLTGGASNL 259 Query: 262 SFGEAALPQAYVLEYELAREQCPRWKAFTQAYMRRYNAAPSPYSYYAYDAASVLIAAILQ 321 S + +VLEYE+AR CP+WK TQ Y RR+ AAP+ YS+YAYDAASVL AAI+Q Sbjct: 260 SLPAPLELRLHVLEYEIARPHCPQWKVVTQTYQRRFGAAPTTYSFYAYDAASVLTAAIVQ 319 Query: 322 SDSTDGEKIAASLRQLRHAGLSGPISFAAGGANAAPRFRLYQYRSRGQWQQVGVFPRGA 380 + S +G+KIA +L ++RHAGL G +SF A GANA PR+ L +Y+ R WQ V VF G+ Sbjct: 320 AGSDNGDKIATALHRMRHAGLHGTLSFDATGANAHPRYTLLRYQER--WQAVEVFAPGS 376 >gi|300310948|ref|YP_003775040.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 394 Score = 161 bits (407), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 107/368 (29%), Positives = 182/368 (49%), Gaps = 9/368 (2%) Query: 16 ACLQPWTMQPAQAEEVRQVTLGLSAWLTQP---IVQSAWHGAELAVEDANTLAAR-KKSS 71 A QP P + +E + +G + L+ P + +S +G +LA+ +AN R S Sbjct: 23 ATAQPRAPLPDR-QETATLAIGFAGPLSGPSGGVGKSMQNGVQLALAEANRRGLRIGGKS 81 Query: 72 WRFRLLAQDDQGSANFGVNVARYFIKEKVAGVIGPWSSDAAMATAELYEAARIPQIGFTA 131 W+ RL+AQDD+ VAR ++++V V+G W+S ++A A +Y A + Q+ +A Sbjct: 82 WQLRLIAQDDRADPATAEFVARSLVQQQVIAVVGHWTSGTSLAAAPVYHRAGVIQVTPSA 141 Query: 132 GTSQWTSQGNRMPFRVVGGTAEVGLTMAEVITTDLKGKRVFVVHNDSAYSNALTDELTAR 191 + + T+ FR + +G A L+ + + + + + + L ++ Sbjct: 142 MSRRLTAMSYPRIFRTIPNNESLGRLAAGYAVDKLETRTIVTIDDRTPFGQGLAEQFART 201 Query: 192 LVNRPGLTATRYLVGRRTTDFNATIKAANQYQADVIVFLALFPQAAAFVEEVRRSGSEAK 251 R RY V +T+DFNA + A + Q D+I F L QA + +RR A+ Sbjct: 202 ASERGAQVVGRYSVSDKTSDFNAALLEARKLQPDLIFFGGLDWQAGVLAKSIRRLKLHAR 261 Query: 252 VLLVGGASSLSFGEAALPQ---AYVLEYELAREQCPRWKAFTQAYMRRYNAAPSPYSYYA 308 ++ G L F A P A VLE +++ P WK+F Q Y +++ Y+ +A Sbjct: 262 LMASPGTLGLPFLMRAGPDANGALVLEPGPPQDKMPGWKSFRQRYSENFDSDMDLYAVFA 321 Query: 309 YDAASVLIAAILQSDSTDGEKIAASLRQLRHAGLSGPISFAAGGANAAPRFRLYQYRSRG 368 Y+AA +I I Q+ S D E+IA ++ +LR G+SGP++F G P F +Y+ + + Sbjct: 322 YEAAQAIIQGIRQAGSADPERIAEAMHRLRFEGVSGPVAFNEEGDLLQPSFTMYEVKDQ- 380 Query: 369 QWQQVGVF 376 +WQ V F Sbjct: 381 RWQPVARF 388 >gi|300312761|ref|YP_003776853.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 380 Score = 108 bits (270), Expect = 8e-25, Method: Compositional matrix adjust. Identities = 84/330 (25%), Positives = 145/330 (43%), Gaps = 23/330 (6%) Query: 52 HGAELAVEDAN----TLAARKKSSWRFRLLAQDDQGSANFGVNVARYFIKEKVAGVIGPW 107 +GA +AV++ N T+ +K +F L+ +DD VA + +KVA VIG Sbjct: 49 NGARMAVDELNAKGFTIGGKK---VKFELVGEDDASDPKQATAVATKLVDQKVAAVIGHL 105 Query: 108 SSDAAMATAELYEAARIPQIGFTAGTSQWTSQGNRMPFRVVGGTAEVGLTMAEVITTDLK 167 +S + +++Y A IPQ+ +A ++T QG + FRVV A++G + + L Sbjct: 106 NSGTTIPASKIYSDAGIPQVSPSATNPKYTQQGFKTAFRVVANDAQLGAALGKYAVQKLG 165 Query: 168 GKRVFVVHNDSAYSNALTDELTARLVNRPGLTATRYLVGRRTTDFNATIKAANQYQADVI 227 K++ V+ + +AY + DE G + TDFNA + + + DV+ Sbjct: 166 TKQIAVIDDRTAYGQGVADEFAKGAKAAGGTIVGTQYTNDKATDFNAILTSLKGKKPDVV 225 Query: 228 VFLALFPQAAAFVEEVRRSGSEAKVLLVGGASSLSFGEAALP----------QAYVLEYE 277 F + + ++++ G AK + G + G +LP Q E Sbjct: 226 FFGGMDAVGGPMLRQMKQLGISAKFM---GGDGICTG--SLPGLAGDGLGDDQVICAEAG 280 Query: 278 LAREQCPR-WKAFTQAYMRRYNAAPSPYSYYAYDAASVLIAAILQSDSTDGEKIAASLRQ 336 E + F AY +++ + YAYDA + A+ ++ S D +K L + Sbjct: 281 GVDEAGKKGMDDFRAAYKKKFGIDVVYNAAYAYDATMTVADAMAKAGSADPKKYLPELAK 340 Query: 337 LRHAGLSGPISFAAGGANAAPRFRLYQYRS 366 + H G++G I+F A G LY Y+ Sbjct: 341 ISHKGVTGVIAFDAKGDIKDGSLTLYTYKG 370 >gi|300309516|ref|YP_003773608.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 378 Score = 106 bits (264), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 86/339 (25%), Positives = 153/339 (45%), Gaps = 21/339 (6%) Query: 26 AQAEEVRQVTLGLSAWLTQPIVQSAWH---GAELAVEDAN----TLAARKKSSWRFRLLA 78 A A+E + V +G S+ LT P + G +A+E N T+ +K +F ++A Sbjct: 22 AMAQETQVVKIGFSSPLTGPQASAGKDNQGGLMMAIERLNAQPITVGGKK---IKFDVIA 78 Query: 79 QDDQGSANFGVNVARYFIKEKVAGVIGPWSSDAAMATAELYEAARIPQIGFTAGTSQWTS 138 +DDQ GV VA+ + V ++GP++S + + +Y A I + A + T Sbjct: 79 EDDQADPKSGVAVAQKLADQGVKAIVGPYNSGVTIPASRVYNDAGI-VVATVASNPKITQ 137 Query: 139 QGNRMPFRVVGGTAEVGLTMAEVITTDLKGKRVFVVHNDSAYSNALTDE-LTARLVNRPG 197 QG FRV +++G MA +LK KRV V+ + +AY L E + N Sbjct: 138 QGFATLFRVAASDSQLGGKMALYAAKELKFKRVAVIDDRTAYGQGLAQEFIKVAKANGID 197 Query: 198 LTATRYLVGRRTTDFNATIKAANQYQADVIVFLALFPQAAAFVEEVRRSGSEAKVLLVGG 257 + +T + + TDF A + + + D + PQ ++++ G + V L+GG Sbjct: 198 VVSTDF-TNDKATDFTAILTSIKGKKPDAVFLGGYAPQGGPIKRQMKQLGVD--VPLMGG 254 Query: 258 ASSLS------FGEAALPQAYVLEYELAREQCPRWKAFTQAYMRRYNAAPSPYSYYAYDA 311 S G+A Y + ++ K F+ Y ++YN Y+ YD Sbjct: 255 DGICSPEMGRLGGDAIGESVYCTQGGTMLDKAKEGKVFSDEYQKKYNRPAETYAVSFYDG 314 Query: 312 ASVLIAAILQSDSTDGEKIAASLRQLRHAGLSGPISFAA 350 ++ A+ Q++S D ++ +L ++ + G++G F A Sbjct: 315 MMLIAQAMKQANSVDPKQFGPALAKISYKGVAGQYDFDA 353 >gi|300311113|ref|YP_003775205.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 383 Score = 73.9 bits (180), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 78/327 (23%), Positives = 139/327 (42%), Gaps = 32/327 (9%) Query: 34 VTLGLSAWLT---QPIVQSAWHGAELAVEDANTLAARKKSSWRFRLLAQDDQGSANFGVN 90 + +G+S T P+ S G +LAV + N AA + +L+ +DD+ GV Sbjct: 26 IKIGVSGPYTGGSAPMGVSMRDGVKLAVAEIN--AAGGLLGRQIQLIERDDEAKNERGVQ 83 Query: 91 VARYFI-KEKVAGVIGPWSSDAAMATAELYEAARIPQIGFTAG----TSQWTSQGNRMPF 145 +A+ I KEKV +G ++ A+A+ Y+ A+IP I A T Q+ + + F Sbjct: 84 IAQELINKEKVVATVGFINTGVALASQRFYQEAKIPVINNVATGTVITKQFEKEKDNYVF 143 Query: 146 RVVGGTAEVGLTMAEVITTDLKGKRVFVVHNDSAYSNALTDELTARLVNRPGLTATRYLV 205 R +A+ K +V ++ + + Y ++L L ++ Sbjct: 144 RTSAADKIQAKMIADEAVDKQKFTKVAILADSTNYGQLGREDLEKVLADKKVTPVAVEKY 203 Query: 206 GRRTTDFNATIKAANQYQADVIVFLALFPQAAAFVEEVRRSGSEAKVLLVGGASSLSFGE 265 + D A + + Q A ++ + P+ A + + ++ G+ LS G Sbjct: 204 NIKDVDMTAQLLKSKQGGAQAVLTYGIGPELAQIANGMEKLNWHVPII---GSWPLSMGN 260 Query: 266 -----------AALPQAYVLEYELAREQCPRWKAFTQAYMRRYNAAPSPYSYYA---YDA 311 A +PQ ++ + P+ KAF +AY + Y P + A YD+ Sbjct: 261 FIDNSGKNGNGARMPQTFIQD-----GNTPKRKAFIEAYQKAYKVDRMPSAVSAAQGYDS 315 Query: 312 ASVLIAAILQSDSTDGEKIAASLRQLR 338 +L AAI Q+ +TDG+K+ A+L L Sbjct: 316 ILLLAAAIKQAGTTDGDKVRAALENLN 342 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.130 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,137,639 Number of extensions: 43632 Number of successful extensions: 173 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 161 Number of HSP's successfully gapped: 8 Length of query: 387 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 300 Effective length of database: 1,207,925 Effective search space: 362377500 Effective search space used: 362377500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)