BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0140.p (550 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313253|ref|YP_003777345.1| methyl-accepting chemotaxis I M... 577 e-165 gi|300312730|ref|YP_003776822.1| methyl-accepting chemotaxis pro... 388 e-109 gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis pro... 388 e-109 gi|300311614|ref|YP_003775706.1| methyl-accepting chemotaxis tra... 385 e-108 gi|300312038|ref|YP_003776130.1| methyl-accepting chemotaxis tra... 382 e-107 >gi|300313253|ref|YP_003777345.1| methyl-accepting chemotaxis I MCP-I serine chemoreceptor transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 556 Score = 577 bits (1486), Expect = e-165, Method: Compositional matrix adjust. Identities = 300/487 (61%), Positives = 380/487 (78%), Gaps = 6/487 (1%) Query: 2 FSKLTIRARLIFVIGFLALMLVGSGVLGLVGLRSTNAQMRSLYEDRLVAFSQLERMSAAL 61 +K +I+ RLI I FL +ML+ SG++GLV L STN +MRSLYEDRLVA++ LE++ + L Sbjct: 1 MNKFSIKIRLILAISFLTIMLLASGIVGLVSLHSTNEKMRSLYEDRLVAYAYLEQIESTL 60 Query: 62 DAARNGISTSIVGDS------GDIDANMDRIEKAIKANDAIVKQYQDTQLTSDERVLLDK 115 ++AR+GI I DS +ID +D + +K + +++ YQ T LT +E LL++ Sbjct: 61 ESARSGIYGIITLDSSEGWDPNEIDRRLDLLVNNLKTVEPVIRSYQATYLTPEESTLLER 120 Query: 116 LIPQRQKFVTDGIKPAAEALRNRDFQGAIELYGGVMLKLYADAHTTLAQLMDLQRTVGRE 175 L+ QRQ + +G+ PA E+LR R FQGA ++Y G M K + H TL+ L +LQ VGRE Sbjct: 121 LLKQRQHYADEGVMPAVESLRKRSFQGADDIYSGAMRKQWDAVHQTLSDLKELQINVGRE 180 Query: 176 LHEQAQQRYQTLLVVMGVAMAAGLFVALLMGVLLVRAISRPLQRAVTVAQAVAAGDLTQQ 235 L+E QQRY+ L+++ +AM AGL VA+L LLVRAIS+PL +AV +AQAVA GDL++Q Sbjct: 181 LYEHEQQRYRQLILMALLAMGAGLIVAVLEAWLLVRAISQPLTQAVGIAQAVAGGDLSRQ 240 Query: 236 IDVHSQDETGQLMQALRQMNDNLQGIVGQVRLSTDTIATASSEIAHGNLDLSSRTEQQAG 295 I+V SQDETGQLMQAL+ M L IV VR T+TI AS EI GNLDLS+RTEQQAG Sbjct: 241 IEVRSQDETGQLMQALKDMTQRLYQIVAVVRQGTETITMASKEITSGNLDLSARTEQQAG 300 Query: 296 ALEETASAMEQLTSTVRHNADSAQQAKELAHSASEVAVRGGEVVRQVVDTMSSINQSSSK 355 +LEETASAM QLTSTV+ NAD+A QA +LA SASEVAVRGGEVVRQVVDTM+SINQSS++ Sbjct: 301 SLEETASAMAQLTSTVKQNADNASQANQLAASASEVAVRGGEVVRQVVDTMTSINQSSTR 360 Query: 356 IVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAAAAKEIKVL 415 IVDII+VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQR+A+AA+EIK L Sbjct: 361 IVDIIAVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRAASAAREIKDL 420 Query: 416 IDDSVTRVGTGSALVAQAGQTIDEVVRSVQRVSEVVTDISAAGQEQSQGIEEVNNAITQM 475 IDDSV +VG GSALV QAG+T++EVV +V++V+++V +IS+A QEQSQGI+E++ AI M Sbjct: 421 IDDSVEKVGKGSALVDQAGKTMEEVVTAVRKVTDMVGEISSASQEQSQGIQEIDRAIVLM 480 Query: 476 DETTQQN 482 D +TQQN Sbjct: 481 DSSTQQN 487 >gi|300312730|ref|YP_003776822.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 583 Score = 388 bits (997), Expect = e-109, Method: Compositional matrix adjust. Identities = 235/486 (48%), Positives = 313/486 (64%), Gaps = 10/486 (2%) Query: 2 FSKLTIRARLIFVIGFLALMLVGSGVLGLVGLRSTNAQMRSLYEDRLVAFSQLERMSAAL 61 F+ L+I RL L L++ G LGL L N + + DR + + +S + Sbjct: 3 FNNLSIATRLAIAFSILILVIAGIVALGLARLADINKALDLVVNDRYKKIAIINTISGKV 62 Query: 62 D----AARNGISTSIVGDSGDIDANMDRIEKAIKANDAIVKQYQDTQLTSDERVLLDKLI 117 D + RN + ++ D I A IE N + + + + LDK+ Sbjct: 63 DDVAISLRNQL---LMTDPAQIAAEQKTIESLSAENTKLYDELTRLVANPEAKAKLDKVT 119 Query: 118 PQRQKFVTDGIKPAAEALRNR-DFQGAIELYGGVMLKLYADAHTTLAQLMDLQRTVGREL 176 R+ + ++ + L++ D AI+L M+ L + L +L D Q+ + + Sbjct: 120 ETRKSYA--ALRKQIDKLQSSGDKDAAIKLMLSEMVPLQKIYFSQLDELSDTQQKLMDQS 177 Query: 177 HEQAQQRYQTLLVVMGVAMAAGLFVALLMGVLLVRAISRPLQRAVTVAQAVAAGDLTQQI 236 +QA+ Y+T ++M +A A + + R+I+RPL RAV VA+ VAAGDLT I Sbjct: 178 VDQAEAAYETTRMIMVIAGVLAATFAAFIAWTISRSITRPLSRAVEVAETVAAGDLTAVI 237 Query: 237 DVHSQDETGQLMQALRQMNDNLQGIVGQVRLSTDTIATASSEIAHGNLDLSSRTEQQAGA 296 HS DETG+L+QAL MN L+ IV +VR TD++ TAS++IA GNLDLSSRTE+QA A Sbjct: 238 AAHSNDETGRLLQALAAMNQKLKAIVLEVRRGTDSMVTASTQIATGNLDLSSRTEEQASA 297 Query: 297 LEETASAMEQLTSTVRHNADSAQQAKELAHSASEVAVRGGEVVRQVVDTMSSINQSSSKI 356 LEETAS++E+LTS+V+ NAD +++ ELA +AS+VA +GG V+QVV TM +IN SS KI Sbjct: 298 LEETASSLEELTSSVKQNADHTRKSSELAANASQVANQGGLAVKQVVQTMGAINDSSHKI 357 Query: 357 VDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAAAAKEIKVLI 416 VDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR LAQRSA AAKEIK LI Sbjct: 358 VDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRGLAQRSATAAKEIKELI 417 Query: 417 DDSVTRVGTGSALVAQAGQTIDEVVRSVQRVSEVVTDISAAGQEQSQGIEEVNNAITQMD 476 +DSV +V +G+ LVAQAG T+D VV S+++VS +V +ISAA +EQS GIE+VN AI QMD Sbjct: 418 NDSVNQVTSGTELVAQAGSTMDAVVNSIEQVSHIVGEISAATREQSDGIEQVNQAIMQMD 477 Query: 477 ETTQQN 482 TTQQN Sbjct: 478 NTTQQN 483 >gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 516 Score = 388 bits (996), Expect = e-109, Method: Compositional matrix adjust. Identities = 248/506 (49%), Positives = 329/506 (65%), Gaps = 48/506 (9%) Query: 1 MFSKLTIRARLIFVIGFLALMLVGSGVLGLVGLRSTNAQMRSLYEDRLVAFSQLERMSAA 60 F+ L I +LI + F A++++ + +LGL +R L A +Q A+ Sbjct: 3 WFNNLKIAKKLI--VSFAAVIVL-TVILGLFAIR------------ELKAVNQ-----AS 42 Query: 61 LDAARNGISTSIVGDSGDIDANMDRIE-------------------KAIKAN-DAIVKQ- 99 D A N + T + + I A++ R+ KAIK+ D + KQ Sbjct: 43 TDMATNWLPT--IKAAQSIQASLPRMRISELQMVTAATPAEREDALKAIKSRLDILAKQR 100 Query: 100 --YQDTQLTSDERVLLDKLIPQRQKFVTDGIKPAAEALRNRDFQGAIELYGGVMLKLYAD 157 Y+ ++E+VL +K ++ + A + ++ + A L+ G K + Sbjct: 101 AAYEAQISEAEEKVLYEKFSKSFATYLEIDKELARISAEGKE-EEARALFKGDSNKTFRS 159 Query: 158 AHTTLAQLMDLQRTVGRELHEQAQQRYQT-LLVVMGVAMAAGLFVALLMGVLLVRAISRP 216 + +++ + + + A+Q Y++ L ++G+ +AA + +AL + V++ RA+SRP Sbjct: 160 MIENMDEIIKVNDAGSKRSDDMAEQVYRSATLWIIGL-LAAIVVIALGLAVMVARAVSRP 218 Query: 217 LQRAVTVAQAVAAGDLTQQIDVHSQDETGQLMQALRQMNDNLQGIVGQVRLSTDTIATAS 276 L AV VA+ VA GDLT I S DETG LM +LR MND+LQ IVG+VR TDTI TAS Sbjct: 219 LNEAVAVAERVAQGDLTMDIRATSTDETGMLMHSLRAMNDSLQQIVGEVRTGTDTITTAS 278 Query: 277 SEIAHGNLDLSSRTEQQAGALEETASAMEQLTSTVRHNADSAQQAKELAHSASEVAVRGG 336 EIA GNLDLSSRTE+QAG+LEETASAME+LT+TV+ NAD+A+QA +LA SAS++A +GG Sbjct: 279 QEIATGNLDLSSRTEEQAGSLEETASAMEELTATVKQNADNARQANQLAASASDIATQGG 338 Query: 337 EVVRQVVDTMSSINQSSSKIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAS 396 VV QVV TM I +SS++I DIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAS Sbjct: 339 AVVGQVVQTMEGITESSNRIADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAS 398 Query: 397 EVRSLAQRSAAAAKEIKVLIDDSVTRVGTGSALVAQAGQTIDEVVRSVQRVSEVVTDISA 456 EVRSLAQRSA+AAKEIK LIDDSV +VG GS LV +AG T+ EVV SV+RV++VV +ISA Sbjct: 399 EVRSLAQRSASAAKEIKQLIDDSVEKVGAGSQLVERAGATMSEVVASVRRVTDVVAEISA 458 Query: 457 AGQEQSQGIEEVNNAITQMDETTQQN 482 A EQS GIEE+N AITQMDE TQQN Sbjct: 459 ASNEQSTGIEEINRAITQMDEVTQQN 484 >gi|300311614|ref|YP_003775706.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 581 Score = 385 bits (990), Expect = e-108, Method: Compositional matrix adjust. Identities = 205/294 (69%), Positives = 247/294 (84%), Gaps = 1/294 (0%) Query: 189 VVMGVAMAAGLFVALLMGVLLVRAISRPLQRAVTVAQAVAAGDLTQQIDVHSQDETGQLM 248 V + +MAA + A+++G L+ R+I +PL AV VA+ VAAGDLT +I+ HS+DETGQLM Sbjct: 194 VTLACSMAA-VLAAIILGYLMARSIVKPLTEAVRVAENVAAGDLTTRIEPHSRDETGQLM 252 Query: 249 QALRQMNDNLQGIVGQVRLSTDTIATASSEIAHGNLDLSSRTEQQAGALEETASAMEQLT 308 QALR+MNDNL IV VR T++I TAS EIA GNLDLS+RTEQQAG+LEETASAME++T Sbjct: 253 QALRKMNDNLIEIVSGVRRGTESITTASGEIASGNLDLSARTEQQAGSLEETASAMEEMT 312 Query: 309 STVRHNADSAQQAKELAHSASEVAVRGGEVVRQVVDTMSSINQSSSKIVDIISVIDGIAF 368 STVR NAD+A+QA ++A SASE+A++GG++V +VV TM INQSS KIVDII VIDGIAF Sbjct: 313 STVRQNADNARQANQMAVSASEIALQGGQIVGRVVHTMDEINQSSRKIVDIIGVIDGIAF 372 Query: 369 QTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAAAAKEIKVLIDDSVTRVGTGSA 428 QTNILALNAAVEAARAGEQGRGFAVVA+EVRSLAQRSAAAAKEIK LI DSV +V GSA Sbjct: 373 QTNILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSAAAAKEIKALIADSVGKVEGGSA 432 Query: 429 LVAQAGQTIDEVVRSVQRVSEVVTDISAAGQEQSQGIEEVNNAITQMDETTQQN 482 LVAQAG T+++VV SV+ V+++V +ISAA EQS GIEE+N A+TQMDE TQQN Sbjct: 433 LVAQAGATMEQVVSSVRSVTDIVAEISAASAEQSSGIEEINRAVTQMDEGTQQN 486 >gi|300312038|ref|YP_003776130.1| methyl-accepting chemotaxis transmembrane I protein [Herbaspirillum seropedicae SmR1] Length = 579 Score = 382 bits (981), Expect = e-107, Method: Compositional matrix adjust. Identities = 244/489 (49%), Positives = 318/489 (65%), Gaps = 14/489 (2%) Query: 1 MFSKLTIRARLIFVIGFLALMLVGSGVLGLVGLRSTNAQMRSLYEDRLVAFSQLERMSAA 60 F L I +LI + M+ GV + L N+ + + L + L R+ A Sbjct: 3 WFYDLRISRKLITTFLVVVAMVAALGVFSIRQLDQVNSASTEISTNWLPSIRTLARLQLA 62 Query: 61 LDAARNGISTSIVGDSGDIDANMDRIEKAIKANDAIVKQYQDTQLTSDERVLLDKLIPQR 120 + R+ I+ + +E+ ++ +K Q +T + P+ Sbjct: 63 MSRLRSFELQHILAKDAK---EYEEVERGMQVQMNNLKTAQAEYVTQISEPEEKAIYPEV 119 Query: 121 QK----FVTDGIKPAAEALRNRDFQGAIELYGGVMLKLYADAHTTLAQLMDLQ--RTVGR 174 +K FV++ K A + + +D + A L G K Y T+L Q+ L G Sbjct: 120 EKLIATFVSEHDKIIALSRQGKD-EEARNLQRGESTKAY---RTSLQQIDKLAAVNDKGA 175 Query: 175 EL-HEQAQQRYQTLLVVMGVAMAAGLFVALLMGVLLVRAISRPLQRAVTVAQAVAAGDLT 233 ++ ++QA Y + + V +AA + +A+L+ V + R I+RPL+ AV+VAQ VA GDLT Sbjct: 176 DISNKQADAIYAQARLWIIVTVAACIGLAMLLAVWVSRLIARPLEEAVSVAQRVAGGDLT 235 Query: 234 QQIDVHSQDETGQLMQALRQMNDNLQGIVGQVRLSTDTIATASSEIAHGNLDLSSRTEQQ 293 +I+ +DETGQL+ AL+ MND+L GIVGQVR+ T TI TAS+EIA GNLDLSSRTE+Q Sbjct: 236 TRIEPAGKDETGQLLVALKSMNDSLVGIVGQVRMGTQTITTASTEIASGNLDLSSRTEEQ 295 Query: 294 AGALEETASAMEQLTSTVRHNADSAQQAKELAHSASEVAVRGGEVVRQVVDTMSSINQSS 353 A +LEETASAME+LTSTV+ NAD+A+QA +LA SASEVA +GG VV QVVDTM SIN SS Sbjct: 296 ASSLEETASAMEELTSTVKQNADNARQANQLAVSASEVASQGGAVVNQVVDTMGSINASS 355 Query: 354 SKIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAAAAKEIK 413 KI DII VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSA AAKEIK Sbjct: 356 RKIADIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAGAAKEIK 415 Query: 414 VLIDDSVTRVGTGSALVAQAGQTIDEVVRSVQRVSEVVTDISAAGQEQSQGIEEVNNAIT 473 LI+ SV +V GS LV +AG T+ EVV+SV+RV+++V +IS+A QEQS+GIE+VN AI Sbjct: 416 ELIEGSVAQVDMGSKLVERAGITMSEVVQSVRRVTDIVGEISSASQEQSEGIEQVNLAIA 475 Query: 474 QMDETTQQN 482 QMDE TQQN Sbjct: 476 QMDEVTQQN 484 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.128 0.339 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,215,042 Number of extensions: 41164 Number of successful extensions: 583 Number of sequences better than 1.0e-08: 70 Number of HSP's gapped: 466 Number of HSP's successfully gapped: 70 Length of query: 550 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 460 Effective length of database: 1,193,720 Effective search space: 549111200 Effective search space used: 549111200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)