BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0128.p (541 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313265|ref|YP_003777357.1| pyruvate dehydrogenase E1 compo... 1032 0.0 gi|300310785|ref|YP_003774877.1| pyruvate dehydrogenase E1 compo... 560 e-160 >gi|300313265|ref|YP_003777357.1| pyruvate dehydrogenase E1 component protein [Herbaspirillum seropedicae SmR1] Length = 893 Score = 1032 bits (2669), Expect = 0.0, Method: Compositional matrix adjust. Identities = 510/537 (94%), Positives = 517/537 (96%) Query: 5 GLCAIGATLTDEEINRLRRGGHDMKKIFSAYQAAASHRGQPTVILAQTKKGYGMGAAGEG 64 G AIGATLTDEEINRLRRGGHDMKKI+SAYQAAA HRGQPTVILAQTKKGYGMGAAGEG Sbjct: 357 GTRAIGATLTDEEINRLRRGGHDMKKIYSAYQAAARHRGQPTVILAQTKKGYGMGAAGEG 416 Query: 65 KMTTHQQKKLDEESLLQFRDRFKLPLTDEQCRELAFYKPADDSPEMKHLHARRAALGGYL 124 KMTTHQQKKLDEESLLQFRDRFKLPL+DEQCR LAFYKPADDSPEMKHLHARRAAL GYL Sbjct: 417 KMTTHQQKKLDEESLLQFRDRFKLPLSDEQCRALAFYKPADDSPEMKHLHARRAALSGYL 476 Query: 125 PRRHAGDARRTVPPLEAHSKFALEADGKEMSSTMALVRQLGNLLKDKDFGQYVVPIVADE 184 PRR AG ARR VPPL AHSKFALEADGKEMSSTMALVRQLGNLLKDKDFGQYVVPIVADE Sbjct: 477 PRRQAGQARRAVPPLAAHSKFALEADGKEMSSTMALVRQLGNLLKDKDFGQYVVPIVADE 536 Query: 185 ARTFGMANLFRQVGIYSSQGQLYEPEDIGSILYYREAKDGQILEEGITEAGAISSWTAAA 244 ARTFGMANLFRQVGIYSSQGQLYEPEDIGSILYYREAKDGQILEEGITEAGAISSWTAAA Sbjct: 537 ARTFGMANLFRQVGIYSSQGQLYEPEDIGSILYYREAKDGQILEEGITEAGAISSWTAAA 596 Query: 245 TAYSVHGTPMLPFYIYYSMFGFQRIGDLIWAAADQRARGFLIGATSGRTTLGGEGLQHQD 304 TAYSVHGTPMLPFYIYYSMFGFQRIGDLIWAAADQRARGFLIGATSGRTTLGGEGLQHQD Sbjct: 597 TAYSVHGTPMLPFYIYYSMFGFQRIGDLIWAAADQRARGFLIGATSGRTTLGGEGLQHQD 656 Query: 305 GNSLVTAASIPNCVSYDPAYAYELAVILDEGMRRMMERGDDVFYYITVTNENEAQPSLPE 364 GNSLVTAASIPNCVSYDPAYAYELAVILDEGMRRMMERGDDVFYYITVTNENEAQPSLPE Sbjct: 657 GNSLVTAASIPNCVSYDPAYAYELAVILDEGMRRMMERGDDVFYYITVTNENEAQPSLPE 716 Query: 365 GVQDGILRGMYCLGRKPGAQVRLLGSGPILKEAVAAAALLQQHWQIDAEVWSVTSYTELA 424 GVQDGILRG+YCL RKP AQ RLLGSGPILKEAVAAAALLQQHWQI+AEVWSVTSYTELA Sbjct: 717 GVQDGILRGLYCLARKPQAQARLLGSGPILKEAVAAAALLQQHWQIEAEVWSVTSYTELA 776 Query: 425 RDGVAAQRAQRLSGSVGQPYVTAQLQDSAAPVIAVSDYIRTLPESIRAFVPAPYVTLGTD 484 RDGVAAQRAQRL+GS PYVT QLQ SA PVIAVSDYIRTLPESIRAFVPAPYVTLGTD Sbjct: 777 RDGVAAQRAQRLAGSAALPYVTQQLQGSAVPVIAVSDYIRTLPESIRAFVPAPYVTLGTD 836 Query: 485 GFGRSDTRVNLRDFFEIDARWTAYTALCEVWGDERARLQEAARTLGIDTNKPLSSSV 541 GFGRSDTRVNLRDFFEIDARW AYTALCEVWGD+RARLQEAARTLGIDT KPLSS+V Sbjct: 837 GFGRSDTRVNLRDFFEIDARWIAYTALCEVWGDDRARLQEAARTLGIDTAKPLSSTV 893 >gi|300310785|ref|YP_003774877.1| pyruvate dehydrogenase E1 component protein [Herbaspirillum seropedicae SmR1] Length = 898 Score = 560 bits (1442), Expect = e-160, Method: Compositional matrix adjust. Identities = 279/543 (51%), Positives = 377/543 (69%), Gaps = 12/543 (2%) Query: 6 LCAIGATLTDEEINRLRRGGHDMKKIFSAYQAAASHRGQPTVILAQTKKGYGMGAAGEGK 65 L + + ++D++I RL RGGHD KI++A++ A + QPTVILA++ KGYG G AGE + Sbjct: 351 LLEMVSKMSDDDIWRLTRGGHDPHKIYAAFKVAQESKDQPTVILAKSIKGYGFGKAGEAR 410 Query: 66 MTTHQQKKLDEESLLQFRDRFKLPLTDEQCRELAFYKPADDSPEMKHLHARRAALGGYLP 125 T H KKLD+E++ RDRF+LP++D + F+KP+DD+PEM++LH RR ALGGYLP Sbjct: 411 NTAHNTKKLDDEAIRAMRDRFQLPISDADLPNIPFFKPSDDAPEMQYLHERRKALGGYLP 470 Query: 126 -RRHAGDARRTVPPLEA-HSKFALEADGKEMSSTMALVRQLGNLLKDKDFGQYVVPIVAD 183 RR D + VP L A + A+G+E+S+T A R L LL+D + G VVPI+ D Sbjct: 471 QRRVQADEKLPVPELSAFQAMLEPTAEGREISTTAAYSRVLTALLRDPNLGPRVVPIMVD 530 Query: 184 EARTFGMANLFRQVGIYSSQGQLYEPEDIGSILYYREAKDGQILEEGITEAGAISSWTAA 243 E+RTFGM LFRQ+GI+S GQLYEP D ++YYRE K GQIL+EGI EAG +SSW AA Sbjct: 531 ESRTFGMEGLFRQIGIFSQVGQLYEPVDKDQVMYYREDKAGQILQEGINEAGGMSSWIAA 590 Query: 244 ATAYSVHGTPMLPFYIYYSMFGFQRIGDLIWAAADQRARGFLIGATSGRTTLGGEGLQHQ 303 AT+YS + M+PFY YYSMFG QRIGDL WAA D RARGFLIG T+GRTTL GEGLQH+ Sbjct: 591 ATSYSTNNRVMIPFYTYYSMFGLQRIGDLAWAAGDMRARGFLIGGTAGRTTLNGEGLQHE 650 Query: 304 DGNSLVTAASIPNCVSYDPAYAYELAVILDEGMRRMMERGDDVFYYITVTNENEAQPSLP 363 DG+S V A++IPNC+ YDP +A+E+AVI+ +G+RRM+ +DVFYYITV NEN P + Sbjct: 651 DGHSHVFASAIPNCIPYDPTFAHEVAVIIHDGLRRMVANQEDVFYYITVMNENYGHPGIK 710 Query: 364 EGVQDGILRGMYCL---GRKPGAQVRLLGSGPILKEAVAAAALLQQHWQIDAEVWSVTSY 420 G ++GI++G+Y L G++ +V+LLGSG IL+E +AAA LL+ W++DA+VWS S+ Sbjct: 711 PGQEEGIIKGLYLLNEGGKQNKLRVQLLGSGTILREVIAAADLLRDDWKVDADVWSAPSF 770 Query: 421 TELARDGVAAQRAQRL--SGSVGQPYVTAQLQDSAAPVIAVSDYIRTLPESIRAFVPA-- 476 T LARDG +R L + + + Y L+ S P++ +DY+RT E +RAFVP Sbjct: 771 TLLARDGQDVERWNMLHPAEAPKKSYFEQSLEGSEGPIVVSTDYMRTYAEQVRAFVPKGR 830 Query: 477 PYVTLGTDGFGRSDTRVNLRDFFEIDARWTAYTALCEVWGDERARLQEAARTL---GIDT 533 Y LGTDG+GRSDTR LR+FFE++ + AL + + + + + A+ + GID Sbjct: 831 SYKVLGTDGYGRSDTRAKLREFFEVNRYFVTVAALKSLADEGKIKPEVVAQAIAKYGIDP 890 Query: 534 NKP 536 NKP Sbjct: 891 NKP 893 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,807,342 Number of extensions: 81999 Number of successful extensions: 276 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 272 Number of HSP's successfully gapped: 2 Length of query: 541 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 451 Effective length of database: 1,193,720 Effective search space: 538367720 Effective search space used: 538367720 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)