BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0111.p (300 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313278|ref|YP_003777370.1| vanillate O-demethylase (vanill... 555 e-159 gi|300310671|ref|YP_003774763.1| flavodoxin reductases (ferredox... 238 5e-64 gi|300309798|ref|YP_003773890.1| flavodoxin reductases (ferredox... 183 2e-47 gi|300313415|ref|YP_003777507.1| phenylacetic acid degradation N... 81 1e-16 gi|300311823|ref|YP_003775915.1| nitric oxide dioxygenase [Herba... 62 7e-11 >gi|300313278|ref|YP_003777370.1| vanillate O-demethylase (vanillate degradation ferredoxin-like) oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 318 Score = 555 bits (1430), Expect = e-159, Method: Compositional matrix adjust. Identities = 275/300 (91%), Positives = 289/300 (96%) Query: 1 VGLELVSVDGAPLPGFTAGAHIDVHLPGGLLRQYSLCNAPHEQHRYQIGVLRDANSRGGS 60 + LELV+VDGA LP FTAGAHIDVHLPGGL+RQYSLCNAPHEQHRYQIGVLRD NSRGGS Sbjct: 19 IALELVAVDGAALPAFTAGAHIDVHLPGGLVRQYSLCNAPHEQHRYQIGVLRDVNSRGGS 78 Query: 61 VAVHEALKVGDLLTIGVPRNQFALVPAQHSLLLAGGIGVTPILCMAEALAASGASFEMHY 120 VAVHEALK+GD+LTI PRNQFALVPAQHSLLLAGGIGVTPILCMAEALAA+GASFEMHY Sbjct: 79 VAVHEALKLGDILTISAPRNQFALVPAQHSLLLAGGIGVTPILCMAEALAAAGASFEMHY 138 Query: 121 CARSPARQAFRERIAGSGFADRVVHHYDDGDAAQKLDLEALLSQADPATHLYVCGPAGFI 180 CARSPARQAFRERIA SGFA RVVHHYDDG+AAQKLDL+ALL+QADPATHLYVCGPAGFI Sbjct: 139 CARSPARQAFRERIAASGFAARVVHHYDDGEAAQKLDLDALLAQADPATHLYVCGPAGFI 198 Query: 181 AHVVETARAHGWPESQVHFEYFGAAPVTADGNGAFDVKLASSGQVYTIPADRTVIQVLCE 240 AHVV+TARA GWPE+QVHFEYFGAAPV ADGNGAF+VKLASSGQVYTIP +RTVIQVLCE Sbjct: 199 AHVVDTARARGWPEAQVHFEYFGAAPVKADGNGAFEVKLASSGQVYTIPPERTVIQVLCE 258 Query: 241 HGVDVPVSCEQGICGTCLTRVLEGEPDHRDQYLTDEERAANDQFTPCCSRARSSLLVLDL 300 HGVDVPVSCEQGICGTCLTRVLEGEPDHRDQYLTDEERAANDQFTPCCSRARS+LLVLDL Sbjct: 259 HGVDVPVSCEQGICGTCLTRVLEGEPDHRDQYLTDEERAANDQFTPCCSRARSALLVLDL 318 >gi|300310671|ref|YP_003774763.1| flavodoxin reductases (ferredoxin-NADPH reductases) family 1 protein [Herbaspirillum seropedicae SmR1] Length = 318 Score = 238 bits (607), Expect = 5e-64, Method: Compositional matrix adjust. Identities = 141/302 (46%), Positives = 179/302 (59%), Gaps = 5/302 (1%) Query: 3 LELVSVDGAPLPGFTAGAHIDVHLPGGLLRQYSLCNAPHEQHRYQIGVLRDANSRGGSVA 62 LV DGAPLP + GAHIDV P G++RQYSLC ++ Y I V R++ SRGGS A Sbjct: 18 FRLVRADGAPLPAYEPGAHIDVTGPTGIMRQYSLCGPLEDRCGYTIAVKRESASRGGSAA 77 Query: 63 VHEALKVGDLLTIGVPRNQFAL-VPAQHSLLLAGGIGVTPILCMAEALAASGASFEMHYC 121 +H+ +K G L +G PRN FAL A LL GIGVTP+L MA L A F +HY Sbjct: 78 LHDVVKAGMELEVGTPRNLFALDASAPEHLLFGAGIGVTPLLAMAYRLWAEEKRFTLHYF 137 Query: 122 ARSPARQAFRERIAGSGFADRVVHHYDDGDAAQKLDLEALLSQADPATHLYVCGPAGFIA 181 R+ AF + + + FA V HY A + L L H+Y CGPA F+ Sbjct: 138 VRAREHAAFADLLENAPFAAHVRFHYAVMPAQMEHYLTVCLEAGSKEAHVYTCGPAPFMD 197 Query: 182 HVVETARAHGWPESQVHFEYF---GAAPVTADGNGAFDVKLASSGQVYTIPADRTVIQVL 238 VV+ A A E +H E+F AAP A G+GAF+VKLASSG+V IP ++ +L Sbjct: 198 AVVKCASA-TRSEEAIHLEHFQAAPAAPAAAGGDGAFEVKLASSGKVVQIPVGIPIVDIL 256 Query: 239 CEHGVDVPVSCEQGICGTCLTRVLEGEPDHRDQYLTDEERAANDQFTPCCSRARSSLLVL 298 G+ + SC +GICGTC+ +LEGEPDHRD L+ +E+AANDQ C SRARS +LVL Sbjct: 257 AREGIVIDTSCREGICGTCVVPLLEGEPDHRDNCLSKKEKAANDQICTCVSRARSGMLVL 316 Query: 299 DL 300 DL Sbjct: 317 DL 318 >gi|300309798|ref|YP_003773890.1| flavodoxin reductases (ferredoxin-NADPH reductases) family 1 protein [Herbaspirillum seropedicae SmR1] Length = 339 Score = 183 bits (464), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 124/310 (40%), Positives = 171/310 (55%), Gaps = 15/310 (4%) Query: 3 LELVSVDGAPLPGFTAGAHIDVHLPGGLLRQYSLCNAPHEQHRYQIGVLRDANSRGGSVA 62 LELVS DGA LP +T GAH+ + L GL+R YSLCN P ++ Y I V R+ +SRGGS Sbjct: 33 LELVSQDGAALPPWTPGAHLRLQLQDGLVRAYSLCNTPQQRDCYLIAVKREPDSRGGSAF 92 Query: 63 VHEALKVGDLLTIGVPRNQFALVP-AQHSLLLAGGIGVTPILCMAEALAASGASFEMHYC 121 +HE ++ G ++ P N F L+ + LLLA GIG+TP+ MA LA G +HY Sbjct: 93 LHEQVQEGTTISASPPVNAFPLIADVRAPLLLAAGIGITPLHAMAAELATQGRRHRLHYF 152 Query: 122 ARSPARQAFRERIAGSGFADRVVHHYDDGDAAQKLDLEALLSQADPATHLYVCGPAGFIA 181 ARS A AF +A +G +H DG A + + A L+ A ++ LYVCGP+ FI Sbjct: 153 ARSLAHAAFFHELA-AGSQGMSLHLGLDG-AETEHAIAAALAAAPRSSPLYVCGPSPFID 210 Query: 182 HVVETARAHGWPESQVHFEYFGAA-----------PVTADGNGAFDVKLASSGQVYTIPA 230 A+ GW E +HFE F A PV A + F++ L SG + Sbjct: 211 AARRHAQLAGWREQDIHFERFAAPAMPAAEADPARPVQAP-DSTFELVLQRSGLRCQVLP 269 Query: 231 DRTVIQVLCEHGVDVPVSCEQGICGTCLTRVLEGEPDHRDQYLTDEERAANDQFTPCCSR 290 ++++ + GV + SC +G CG+C + VLEG+P HRD L+ ERA+ + PC SR Sbjct: 270 GQSIVAAAAQVGVVIGTSCGEGFCGSCESTVLEGQPWHRDSVLSAAERASGRRILPCVSR 329 Query: 291 ARSSLLVLDL 300 + LVLDL Sbjct: 330 CAGTRLVLDL 339 >gi|300313415|ref|YP_003777507.1| phenylacetic acid degradation NADH oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 356 Score = 80.9 bits (198), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 82/310 (26%), Positives = 135/310 (43%), Gaps = 49/310 (15%) Query: 30 LLRQYSLCNAPHEQHRYQIGVLRDANSR--GG--SVAVHEALKVGDLLTIGVPRNQFALV 85 L R YS+C+A E+ LR A R GG S +E+ G + + P F V Sbjct: 53 LRRSYSICSAVQERQ------LRVAIKRAPGGLFSNWANESFVPGQRIDVMPPMGHFN-V 105 Query: 86 PAQ-----HSLLLAGGIGVTPILCMAEA--LAASGASFEMHYCARSPARQAFRERIA--G 136 P + H L A G G+TP++ + + L+ + F + Y R+ + F+E + Sbjct: 106 PLEAGNRKHYLAFAAGSGITPMMSIIKTTLLSEPHSHFTLVYANRASSSVIFKEELTDLK 165 Query: 137 SGFADRVVHHYDDGDAAQKLDL----------EAL------LSQADPATHLYVCGPAGFI 180 + +R Y Q ++L +A L D A ++CGP + Sbjct: 166 DAYLERFNVVYVMSREQQDVELFNGRIDRAKCDAFFASWIDLKDVDAA---FLCGPEEMV 222 Query: 181 AHVVETARAHGWPESQVHFEYFGA-------APVTADGNGAFDVKLASSG--QVYTIPAD 231 V + +AHG P++Q+ E F A A T G +V + G V+T+ + Sbjct: 223 QAVAASLQAHGLPKTQIKTELFAASTPPRAHAARTVVGKQECEVTVIVDGYHNVFTMDKE 282 Query: 232 R-TVIQVLCEHGVDVPVSCEQGICGTCLTRVLEGEPDHRDQYLTDEERAANDQFTPCCSR 290 + +V++ +HG+D+ SC+ G+C TC +V+EG+ D Y ++ A C S Sbjct: 283 KESVLEAGLKHGIDLRYSCKGGVCATCRCKVVEGKVDMDANYALEDYEIARGFVLSCQSF 342 Query: 291 ARSSLLVLDL 300 S L+LD Sbjct: 343 PVSDKLLLDF 352 >gi|300311823|ref|YP_003775915.1| nitric oxide dioxygenase [Herbaspirillum seropedicae SmR1] Length = 397 Score = 61.6 bits (148), Expect = 7e-11, Method: Compositional matrix adjust. Identities = 64/222 (28%), Positives = 99/222 (44%), Gaps = 24/222 (10%) Query: 5 LVSVDGAPLPGFTAGAHIDVHLP---GGLLRQYSLC---NAPHEQHRYQIGVLRDANSRG 58 V DG L F G +I + + RQYSL + +Y+I V R+ G Sbjct: 175 FVPQDGGELLDFAPGQYIGLRMVVDGEEQRRQYSLSALLRQAGQPPQYRISVKREP---G 231 Query: 59 GSVA--VHEALKVGDLLTIGVPRNQFALVPAQHSL-LLAGGIGVTPILCMAEALAASGAS 115 G V+ +H+ + VG ++ + P +F L+ + L L++GG+G+TP++ M A SG Sbjct: 232 GKVSRQLHDQIHVGSVVELFPPAGEFTLMASDKPLALISGGVGITPMMSMLAAALPSGRP 291 Query: 116 FEMHYCARSPARQAFR---ERIAGSGFADRVVHHYD---DGDA---AQKLDLEALLSQ-- 164 + AR AFR E +A ++ Y+ GDA A L +LL Q Sbjct: 292 IHFIHAARHGGVHAFRRQLEELAARHPQLQLRFCYEQPRQGDAAPHATGLIERSLLQQWL 351 Query: 165 -ADPATHLYVCGPAGFIAHVVETARAHGWPESQVHFEYFGAA 205 +P Y GP F+ + G P +Q +E+FG A Sbjct: 352 PGNPDLDAYFVGPKSFMKAIKRQLAELGVPAAQSRYEFFGPA 393 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,046,720 Number of extensions: 47194 Number of successful extensions: 140 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 133 Number of HSP's successfully gapped: 6 Length of query: 300 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 215 Effective length of database: 1,217,395 Effective search space: 261739925 Effective search space used: 261739925 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)