BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0098.p (373 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313291|ref|YP_003777383.1| porin transmembrane protein [He... 627 0.0 gi|300310614|ref|YP_003774706.1| outer membrane porin [Herbaspir... 322 3e-89 gi|300311557|ref|YP_003775649.1| outer membrane porin [Herbaspir... 156 3e-39 gi|300313813|ref|YP_003777905.1| transmembrane outer membrane po... 151 9e-38 gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) ... 138 7e-34 >gi|300313291|ref|YP_003777383.1| porin transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 365 Score = 627 bits (1616), Expect = 0.0, Method: Compositional matrix adjust. Identities = 316/342 (92%), Positives = 333/342 (97%) Query: 32 QSSVTIYGIIDTGVVYTTNANANGNSVFKMPSLTGSFPSRIGFRGTEDLGGGLQALFVLE 91 QSSVTIYGIIDTGVVYTTNANANGNSVFKMPSLTGSFPSRIGF+GTEDLGGGLQA+FVLE Sbjct: 24 QSSVTIYGIIDTGVVYTTNANANGNSVFKMPSLTGSFPSRIGFKGTEDLGGGLQAMFVLE 83 Query: 92 SGFAPDTGAMGQGGRLFGRQSYVGLKSGWGQIMLGRQLNMTYISQLKTDVMGPNIFAIGS 151 SGFAPDTGAMGQGGRLFGRQSYVGLK+ WGQ+MLGRQ+NMTY+SQLKTDVMGPN+FAIGS Sbjct: 84 SGFAPDTGAMGQGGRLFGRQSYVGLKNSWGQLMLGRQVNMTYLSQLKTDVMGPNLFAIGS 143 Query: 152 IDPYLPNARSDNAIGYLGNFSGFTVGATYSFGRDAAGPAGPAATNCAGEVAGNSKACRQV 211 ID YLPNARSDNAIGYLGNFSGFTVGATYSFGRDAAGPAGPAATNC GEVAGNSKACRQV Sbjct: 144 IDAYLPNARSDNAIGYLGNFSGFTVGATYSFGRDAAGPAGPAATNCPGEVAGNSKACRQV 203 Query: 212 TGLLGYDGKGWGFTSSYDILYGNTGASNGLTSSDNSDQRITLNGYYMIGDVKIGAGVVDR 271 T LLGYDGKGWG TSSYDILYGNTGASNG+TSSDN+DQR+TLNGY+M+GDVKIG GVVDR Sbjct: 204 TALLGYDGKGWGVTSSYDILYGNTGASNGMTSSDNTDQRVTLNGYFMLGDVKIGGGVVDR 263 Query: 272 RTRAAANTNTDSDLYYLGVSYPLTTTLVLDAQVSRLDVKNSPNDVTLSVARLTYNLSKRT 331 RTRAAANTNTDSDLYYLGVSYPL+ LVLDAQVSRLDVKNSPNDVTLSVARLTYNLSKRT Sbjct: 264 RTRAAANTNTDSDLYYLGVSYPLSVALVLDAQVSRLDVKNSPNDVTLSVARLTYNLSKRT 323 Query: 332 ALYTSLGYIKNGGTSAVAIDAGGTVGAGKNQLGVMSGIRHTF 373 A+Y SLGYIKNGGTS+VA+DAGGTVGAGKNQLGVM+GIRHTF Sbjct: 324 AVYGSLGYIKNGGTSSVALDAGGTVGAGKNQLGVMTGIRHTF 365 >gi|300310614|ref|YP_003774706.1| outer membrane porin [Herbaspirillum seropedicae SmR1] Length = 369 Score = 322 bits (826), Expect = 3e-89, Method: Compositional matrix adjust. Identities = 178/370 (48%), Positives = 239/370 (64%), Gaps = 12/370 (3%) Query: 13 ALAAAACAMTAGAAGSAMAQSSVTIYGIIDTGVVYTTNAN---ANGNSVFKMPSLTGSFP 69 AL A A+ +A + AQS VT+YGIIDTGV Y +N + A + ++P+LT + P Sbjct: 3 ALKAIIAALLTMSATLSQAQS-VTLYGIIDTGVEYVSNVSNVGAGQQGLLRVPNLTATTP 61 Query: 70 SRIGFRGTEDLGGGLQALFVLESGFAPDTGAMGQGGRLFGRQSYVGLKSGWGQIMLGRQL 129 SRIG RG+EDLGGGL+++F+LESGFAPD+GA+ QGGR+FGR +YVGL WGQ+ GRQ Sbjct: 62 SRIGLRGSEDLGGGLRSVFMLESGFAPDSGALNQGGRMFGRIAYVGLSDHWGQVSFGRQA 121 Query: 130 NMTYISQLKTDVMGPNIFAIGSIDPYLPNARSDNAIGYLGNFSGFTVGATYSFGRDAAGP 189 M + L +D++G N++ ++D Y PNAR+DN+I Y G+F FT+G TYSFGRD Sbjct: 122 TMLLWATLDSDILGANVYGANALDNYRPNARADNSIAYRGSFGAFTLGGTYSFGRDTVNA 181 Query: 190 -AGPAATNCAGEVAGNSKACRQVTGLLGYDGKGWGFTSSYDILYGNTGASNGLTSSDNSD 248 PA TNCAGE AG+S ACR+ + ++ YD WG + +YD L G GA+ GL+SS SD Sbjct: 182 GQNPAGTNCAGEQAGDSLACREWSLMVKYDTAAWGASLAYDTLRGGKGAAAGLSSSQLSD 241 Query: 249 QRITLNGYYMIGDVKIGAGVVDRRTRAAANTNTDSDLYYLGVSYPLTTTLVLDAQVSRLD 308 R+ L Y +K+G G R + +A + S LYY G+SY LT TL L Q+ L Sbjct: 242 SRMLLGAYIKADRLKVGIGGSLRHNQGSALSK--SQLYYAGLSYDLTPTLNLAGQLYYLH 299 Query: 309 VKNSPNDVTLSVARLTYNLSKRTALYTSLGYIKNGGTSAVAIDAGGTVGA-----GKNQL 363 +S N L R TY+LS RTALY + G+I N G S +++ AGG+VG G NQ Sbjct: 300 YDDSANKAWLGALRATYSLSARTALYLTGGFIDNRGQSNLSVSAGGSVGTANPAPGGNQF 359 Query: 364 GVMSGIRHTF 373 G+M+GIRH+F Sbjct: 360 GMMAGIRHSF 369 >gi|300311557|ref|YP_003775649.1| outer membrane porin [Herbaspirillum seropedicae SmR1] Length = 374 Score = 156 bits (394), Expect = 3e-39, Method: Compositional matrix adjust. Identities = 138/384 (35%), Positives = 187/384 (48%), Gaps = 63/384 (16%) Query: 27 GSAMAQSSVTIYGIIDTGVVYTTNANANGNSVFKMPSLTGSFP-SRIGFRGTEDLGGGLQ 85 G+A AQS+VTIYGI+DT + Y +NANA GNS +M + G+ SRIGF+GTEDLGGGL+ Sbjct: 17 GAAFAQSNVTIYGIVDTSIHYLSNANAQGNSNVRMDN--GAIANSRIGFKGTEDLGGGLK 74 Query: 86 ALFVLESGFAPDTGAMGQGGRLFGRQSYVGLKSGWGQIMLGRQLNMTYISQLKTDVMGPN 145 A+F LE+GF+ DTGAM QGG LFGRQS+VGL+ G+G++ +GRQ T + L D P Sbjct: 75 AMFQLENGFSSDTGAMQQGGSLFGRQSWVGLQGGFGKVRVGRQ--NTPLFDLLADHFDPL 132 Query: 146 IFAIGSIDPYLPNA----RSDNAIGYLGNFSGFTVGATYSFGRDAAG------------- 188 + + +LP A RS N + Y G+F A+++FG A Sbjct: 133 TVGNYASNSWLPAAASLVRSPNMLRYDGDFGPVAASASWAFGEKAGSVRTGSQFSSALRY 192 Query: 189 PAGPAATNCAGE--VAGNSKACRQVTGLLGYDGKGWGFTSSYDI----LYG---NTGASN 239 AGP A + V+ + + + T W ++SYD ++G N + Sbjct: 193 TAGPLALGGGYQQTVSSTNSSLKHTT---------WNLSASYDFGVAKVFGGWLNIKDDS 243 Query: 240 GLTSSDNSDQRITLNGYYMIGDVKIGAGVVDRRTRAAANTNTDSDLYYLGVSYPLTTTLV 299 GLT S + NG+ G G+ V R+ ++LG SY T L Sbjct: 244 GLTGSVMAADGDVSNGWTTAGAANNGSTGVKRKDNG----------FFLGSSYKATNALT 293 Query: 300 LDAQVSRLDVKNSPNDVTLSV---ARLT------YNLSKRTALYTSLGYIKNGGTSAVAI 350 L KN T +V R T Y LSKRT LYT++ Y K A+ Sbjct: 294 LTGAFYYDRSKNVSYVSTGNVGDGKRYTLVGWADYALSKRTQLYTTIDYNK---AKDAAV 350 Query: 351 DAGGTVGAGKNQLGVMS-GIRHTF 373 GKN L + GIRH F Sbjct: 351 YELTNSANGKNSLTTVGVGIRHIF 374 >gi|300313813|ref|YP_003777905.1| transmembrane outer membrane porin signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 354 Score = 151 bits (381), Expect = 9e-38, Method: Compositional matrix adjust. Identities = 130/362 (35%), Positives = 177/362 (48%), Gaps = 39/362 (10%) Query: 27 GSAMAQSSVTIYGIIDTGVVYTTNANANG-NSVFKMPSLTGSFPSRIGFRGTEDLGGGLQ 85 GS AQSSVTIYG++DTGV Y NA NG SV +M S + SRIGFRGTEDLGGGL+ Sbjct: 17 GSVQAQSSVTIYGLVDTGVEYLNNAGPNGAGSVTRMTS-GATNTSRIGFRGTEDLGGGLK 75 Query: 86 ALFVLESGFAPDTGAM-GQGGRLFGRQSYVGLKSGWGQIMLGRQLNMTYISQLKTDVMGP 144 ALF LE+GF D G+ G +LF RQS VGL+ G+G+++ GR + TY D MG Sbjct: 76 ALFQLENGFKLDNGSFDGDANQLFNRQSTVGLEGGFGRVVAGRSYSTTYDFLGPFDPMGY 135 Query: 145 NIF--------AIGSIDPYLPNARSDNAIGYLGNFSGFTVGATYSFGRDAAGPAGPAATN 196 + A G+ +P S N + Y G FSG +GA Y F Sbjct: 136 SANYSWATTAGATGNRKDGMPTGVS-NLVKYQGEFSGVKIGAMYGF-------------- 180 Query: 197 CAGEVAGNSKACRQVTGLLGYDGKGWGFTSSYDILYGNTGASNGLTSSDNSDQRITLNGY 256 GEVAG ++ + LG+ + ++YD N A+ T D + GY Sbjct: 181 --GEVAGETRNGAKYGVGLGWGTGPFSVVATYDR---NNSATATNTPYDRATSAHLAAGY 235 Query: 257 YMIGDVKIGAGV-VDRRTRAAANTNTDSDLYYLGVSYPLTTTLVLDAQVSRLDVKNSPND 315 K G +++ A + SD ++ G +Y T + L + ++KN ND Sbjct: 236 QPSEAWKFNLGYRYYKKSLATGAADLRSDFFWGGAAYKFTPAMTLIGAIYYQNIKNVAND 295 Query: 316 V--TLSVARLTYNLSKRTALYTSLGY--IKNGGTSAVAIDAGGTVGAGKNQLGVMSGIRH 371 + RL Y +SKRT LY S Y KNG ++ D G G +Q G+ G++H Sbjct: 296 ADPIMYSLRLKYAMSKRTDLYLSTAYAKAKNGQLVGLSRD---DAGYGSSQFGMTLGMQH 352 Query: 372 TF 373 F Sbjct: 353 RF 354 >gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 352 Score = 138 bits (348), Expect = 7e-34, Method: Compositional matrix adjust. Identities = 129/373 (34%), Positives = 175/373 (46%), Gaps = 46/373 (12%) Query: 19 CAMTAGAAGSAMAQSSVTIYGIIDTGVVYTTNANANGNSVFKMPSLTGSFP--SRIGFRG 76 AM AG A +A AQSSV+IYG+ID G+ Y ++A +G ++ + SRIGF+G Sbjct: 8 AAMLAGGATTAHAQSSVSIYGLIDLGLTYYSSAPKSGGGGARLLRMDSGIAQGSRIGFKG 67 Query: 77 TEDLGGGLQALFVLESGFAPDTGAMGQGGRLFGRQSYVGLKS-GWGQIMLGRQLN-MTYI 134 TEDLGGG+ A F LE+GF D G++GQGG LFGRQS+VGL S WG + +GRQ + M I Sbjct: 68 TEDLGGGMNAFFTLETGFGADDGSLGQGGLLFGRQSFVGLGSTQWGSLSVGRQYDFMANI 127 Query: 135 -SQLKTDVMGP------NIFAIGSIDPYLPNA-----RSDNAIGY-LGNFSGFTVGATYS 181 Q + P + A + L N R++N+I Y F GF+ GA Sbjct: 128 GGQYAMGALSPAGSFGWGLHADAAHGTALNNHIYAGDRTNNSIKYNSAKFGGFSFGAMVG 187 Query: 182 FGRDAAGPAGPAATNCAGEVAGNSKACRQVTGLLGYDGKGWGFTSSYDILYGNTGASNGL 241 GE AGN+ A R V+ YD + +Y + +TG+ + Sbjct: 188 L----------------GETAGNNNAGRTVSATGQYDNGPFSGALAYTDIKDSTGSFS-- 229 Query: 242 TSSDNSDQRITLNG-YYMIGDVKIGAGVVDRRTRAAANTNTDSDLYYLGVSYPLTTTLVL 300 RI G Y IG GVV + NT+ + Y LG Y LT L Sbjct: 230 -------TRIYGGGARYQIGAFTP-FGVVTQVKN--TNTSAKATTYELGTVYALTPLWDL 279 Query: 301 DAQVSRLDVKNSPNDVTLSVARLTYNLSKRTALYTSLGYIKNGGTSAVAIDAGGTVGAGK 360 A + VA + Y +SKRT LY Y ++ G +A + GG A Sbjct: 280 AAAYQFQKRNQGVGNAQALVATVDYKISKRTDLYVGAVYDRDKGYNAYPVFGGGIQSATG 339 Query: 361 NQLGVMSGIRHTF 373 NQ + G+RH F Sbjct: 340 NQTALRLGMRHRF 352 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.133 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,094,050 Number of extensions: 48352 Number of successful extensions: 185 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 144 Number of HSP's successfully gapped: 14 Length of query: 373 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 286 Effective length of database: 1,207,925 Effective search space: 345466550 Effective search space used: 345466550 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)