BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0095.p (264 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313293|ref|YP_003777385.1| enoyl-CoA hydratase/carnithine ... 491 e-140 gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspiri... 92 4e-20 gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [... 88 7e-19 gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspiri... 77 1e-15 gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein ... 64 9e-12 >gi|300313293|ref|YP_003777385.1| enoyl-CoA hydratase/carnithine racemase [Herbaspirillum seropedicae SmR1] Length = 259 Score = 491 bits (1263), Expect = e-140, Method: Compositional matrix adjust. Identities = 240/259 (92%), Positives = 248/259 (95%) Query: 6 MNQHSDLLDIQVHGAIAEIALNRAAKRNALNDPLVRAIRDCFQDLPEGVKVAIIHGIGEH 65 MNQHS+LLDIQ HGAIAEI LNRAAKRNALNDPLVRAIRDCF++LPEGVKVAI+ G GEH Sbjct: 1 MNQHSELLDIQQHGAIAEITLNRAAKRNALNDPLVRAIRDCFENLPEGVKVAILRGHGEH 60 Query: 66 FCAGLDLSELRERDTTESLHHSRMWHAAFEQIAFGRVPVIAVLHGAVIGGGLELASAAHI 125 FCAGLDLSELRERDTTESLHHSRMWHAAFEQIAFGRVPVIAVLHGAVIGGGLELA+AAHI Sbjct: 61 FCAGLDLSELRERDTTESLHHSRMWHAAFEQIAFGRVPVIAVLHGAVIGGGLELAAAAHI 120 Query: 126 RVAEPSAFYGLPEGQRGLFVGGGGSVRISRLIGVARMADMMLTGRVLTAEEGHQAGISQY 185 RVAEPS FYGLPEGQRGLFVGGGGSVRISRLIGVARM DMMLTGRVL+AEEGHQAGI QY Sbjct: 121 RVAEPSTFYGLPEGQRGLFVGGGGSVRISRLIGVARMTDMMLTGRVLSAEEGHQAGIPQY 180 Query: 186 SVAAGTGLDKARELAERIARNAPMTNYGIMHVLPRIHDQSIQDGLMTESLMAAVAGSDPD 245 SV AG G+DKARELAERIARNAPMTNYG+MHVLPRIHDQSIQDGLMTESLMAAVAGSDPD Sbjct: 181 SVDAGAGMDKARELAERIARNAPMTNYGVMHVLPRIHDQSIQDGLMTESLMAAVAGSDPD 240 Query: 246 TKGRLAAFLDHKQNKVKPT 264 TKGRLAAFLD KQNKVKP Sbjct: 241 TKGRLAAFLDLKQNKVKPV 259 >gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 258 Score = 92.4 bits (228), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 75/255 (29%), Positives = 122/255 (47%), Gaps = 9/255 (3%) Query: 13 LDIQVHGAIAEIALNRAAKRNALNDPLVRAIRDCFQ--DLPEGVKVAIIHGIGEHFCAGL 70 + ++ H + I L+R NAL+D L+ + + D E + II G + F AG Sbjct: 6 IQVETHEKVGLIRLHRPKALNALSDGLMTELGEALLAFDAQEEIGCIIITGSEKAFAAGA 65 Query: 71 DLSELRERDTTESLHH---SRMWHAAFEQIAFGRVPVIAVLHGAVIGGGLELASAAHIRV 127 D+S + D + +R W E + R PVIA + G +GGG ELA + Sbjct: 66 DISAMAGYDYMDVFKGEFITRNW----ETLRRIRKPVIAAVAGYALGGGCELAMMCDFII 121 Query: 128 AEPSAFYGLPEGQRGLFVGGGGSVRISRLIGVARMADMMLTGRVLTAEEGHQAGISQYSV 187 A +A +G PE + G+ G GG+ R+ R + A+ D+ LTGR++ AEE +AG+ V Sbjct: 122 AADNAKFGQPEIKLGIVPGAGGTQRLPRAVSKAKAMDLALTGRMMDAEEAERAGLVSRIV 181 Query: 188 AAGTGLDKARELAERIARNAPMTNYGIMHVLPRIHDQSIQDGLMTESLMAAVAGSDPDTK 247 AA L++A A IA + + R ++ ++ +G+ E + + D K Sbjct: 182 AADKLLEEAMAAAIIIAEMPRQVAMMVKDSVNRAYETTLSEGMKYERALFYSCFATEDQK 241 Query: 248 GRLAAFLDHKQNKVK 262 + AFL+ +Q K Sbjct: 242 EGMKAFLEKRQPVFK 256 >gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [Herbaspirillum seropedicae SmR1] Length = 262 Score = 87.8 bits (216), Expect = 7e-19, Method: Compositional matrix adjust. Identities = 80/258 (31%), Positives = 126/258 (48%), Gaps = 18/258 (6%) Query: 9 HSDLLDIQVHGA-IAEIALNRAAKRNALNDPLVRAIRDCFQDLPE--GVKVAIIHGIGEH 65 + DLL + HG + + LNR A RNAL + +R I E V+V +I G + Sbjct: 7 YEDLLAAR-HGEHVLHLTLNRPALRNALRNQSLREIVAALARAEEDDSVRVVVISGNEKA 65 Query: 66 FCAGLDLSELRERDT--TESLHHSRMWHAAFEQIAFGRVPVIAVLHGAVIGGGLELASAA 123 F AG DL+E+ +D T+ ++ W A IA P++A ++G +G G EL A Sbjct: 66 FAAGADLNEMIHKDAIATQLDVRAQYWRA----IARFPKPILAAVNGYALGAGCELLMHA 121 Query: 124 HIRVAEPSAFYGLPEGQRGLFVGGGGSVRISRLIGVARMADMMLTGRVLTAEEGHQAGIS 183 I +A A G PE G G GG+ R+ R +G M+L+G ++A++ QAG+ Sbjct: 122 DIAIAARGAKIGQPEINVGTLPGAGGTQRLIRTVGKPLAMKMVLSGEFISADQALQAGLV 181 Query: 184 QYSVAAGTGLDKARELAERIARNAPMTNYGIMHVLPRIHDQSIQDGLMTE----SLMAAV 239 V L++ LA IA+ +P+ + + + ++ GL+ E SLMAA Sbjct: 182 AEVVDDDATLERTLALAHSIAQKSPLAVRLAKEAMLQSFELGLEAGLLFERKSFSLMAAS 241 Query: 240 AGSDPDTKGRLAAFLDHK 257 A D + +AAF + + Sbjct: 242 A----DRQEGIAAFQEKR 255 >gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 77.4 bits (189), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 65/232 (28%), Positives = 105/232 (45%), Gaps = 4/232 (1%) Query: 32 RNALNDPLVRAIRDCFQDLPEGVKVAIIHGIGEHF-CAGLDLSELRERDTTESLHHSRMW 90 R AL D L R +++ D + ++ G GE F AG DL + D + +R + Sbjct: 30 RAALAD-LTRLVKELNAD--RDIYTLVVTGEGEKFFSAGADLKLFADGDKAMAREMARRF 86 Query: 91 HAAFEQIAFGRVPVIAVLHGAVIGGGLELASAAHIRVAEPSAFYGLPEGQRGLFVGGGGS 150 AFE ++ R IA ++G +GGGLE A A IR+AE A LPE GL GG+ Sbjct: 87 GEAFETLSAFRGVSIAAINGYAMGGGLECALACDIRIAEEQAQMALPEAAVGLLPCAGGT 146 Query: 151 VRISRLIGVARMADMMLTGRVLTAEEGHQAGISQYSVAAGTGLDKARELAERIARNAPMT 210 + L+G M+L G + AE + G+ + V G ++A LA++ + +P + Sbjct: 147 QNLPWLVGEGWAKRMILCGERVNAETALRIGLVEEVVPRGQARERALALAQQAEKQSPSS 206 Query: 211 NYGIMHVLPRIHDQSIQDGLMTESLMAAVAGSDPDTKGRLAAFLDHKQNKVK 262 ++ + L TE + D + + AFL+ + + K Sbjct: 207 VAASKQLIQGARHNPLGTILQTERELFVDLFDTQDQREGVQAFLEKRSPQWK 258 >gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein [Herbaspirillum seropedicae SmR1] Length = 263 Score = 64.3 bits (155), Expect = 9e-12, Method: Compositional matrix adjust. Identities = 56/191 (29%), Positives = 86/191 (45%), Gaps = 6/191 (3%) Query: 21 IAEIALNRAAKRNALNDPLVRAIRDCFQDLP--EGVKVAIIHGIGEHFCAGLDLSE--LR 76 IA I LNR K N+ + +RD L V+V ++ G G FCAG DL + ++ Sbjct: 14 IAVITLNRPDKLNSFTVAMHLELRDAIATLQADPSVRVLLLTGAGRGFCAGQDLGDRAVK 73 Query: 77 ERDTTESLHHS--RMWHAAFEQIAFGRVPVIAVLHGAVIGGGLELASAAHIRVAEPSAFY 134 D L S + + + + PVI ++G G G L A I +A SA + Sbjct: 74 PGDDGVDLGESIDKYYGPLVKSLRALPFPVICAVNGVAAGAGANLPLACDIVLAARSASF 133 Query: 135 GLPEGQRGLFVGGGGSVRISRLIGVARMADMMLTGRVLTAEEGHQAGISQYSVAAGTGLD 194 + GL GG+ + RL+G AR M L G ++AE+ + G+ V ++ Sbjct: 134 VEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAERWGLIWKCVDDDQLME 193 Query: 195 KARELAERIAR 205 +A +A AR Sbjct: 194 EAHAMAAHFAR 204 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 833,919 Number of extensions: 36322 Number of successful extensions: 142 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 142 Number of HSP's successfully gapped: 6 Length of query: 264 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 181 Effective length of database: 1,226,865 Effective search space: 222062565 Effective search space used: 222062565 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)