BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0094.p (621 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313294|ref|YP_003777386.1| long chain acyl-CoA synthetase ... 1224 0.0 gi|300311882|ref|YP_003775974.1| long-chain acyl-CoA synthetase ... 95 2e-20 gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligas... 68 2e-12 >gi|300313294|ref|YP_003777386.1| long chain acyl-CoA synthetase [Herbaspirillum seropedicae SmR1] Length = 620 Score = 1224 bits (3167), Expect = 0.0, Method: Compositional matrix adjust. Identities = 589/621 (94%), Positives = 609/621 (98%), Gaps = 1/621 (0%) Query: 1 MNTPPRYRSFRFGIGGVDVVPGAEGISIVKTRQPLGDYPARLTDKLIHWATVDPDRTYMA 60 MNTPPRYRSFRFGIGGVDVVPGA+G+SIVKTRQPL YPARLTDKLIHWATVDPDRTYMA Sbjct: 1 MNTPPRYRSFRFGIGGVDVVPGADGVSIVKTRQPLDAYPARLTDKLIHWATVDPDRTYMA 60 Query: 61 RRDKDGQWRRISYGQALQSARRIGQALLQRGLSAERPLLILSENDLEHAMLVLGCHYAGV 120 RRD+DGQWRRISYGQALQSARRIGQALLQRGLSAERPLLILSENDLEHAMLVLGCHYAGV Sbjct: 61 RRDQDGQWRRISYGQALQSARRIGQALLQRGLSAERPLLILSENDLEHAMLVLGCHYAGV 120 Query: 121 PYAPVSSAYSLLSKDYAKLRHAVQLLTPGLIFAAHGEKFAAAVNAVAPDIEFVVTEAPPA 180 PYAPVSSAYSLLSKDYAKLRHAVQLLTPGLIFAAHGEKFAAAVNAVAP+IEFVVTEAPPA Sbjct: 121 PYAPVSSAYSLLSKDYAKLRHAVQLLTPGLIFAAHGEKFAAAVNAVAPEIEFVVTEAPPA 180 Query: 181 GRECTLYAELEATRAGDEVERAYAATGPDTIVKFLFTSGSTKMPKAVVNTQRMMCSNLQM 240 GRECTLYAELEATRAGDEVERA+AATGP+TIVKFLFTSGSTKMPKAV+NTQRMMCSNLQM Sbjct: 181 GRECTLYAELEATRAGDEVERAFAATGPETIVKFLFTSGSTKMPKAVINTQRMMCSNLQM 240 Query: 241 IVQTFPFLAEEPPILIDWLPWNHTFGANHNVGIVIYNGGTMYIDEGKPTPQGLATTLANL 300 IVQTFPFL EEPPILIDWLPWNHTFGANHNVGIV+YNGGTMYIDEGKPTPQGLATTLANL Sbjct: 241 IVQTFPFLGEEPPILIDWLPWNHTFGANHNVGIVLYNGGTMYIDEGKPTPQGLATTLANL 300 Query: 301 HEISPTVYFNVPVGWEGIAHALEKDQALREKFYSRLRMQFYAGAALAQPVWDKLHATAEA 360 H+ISPTVYFNVPVGWEGIAHALEKDQALREKFYSRLRMQFYAGAALAQPVWDKLHATAEA Sbjct: 301 HDISPTVYFNVPVGWEGIAHALEKDQALREKFYSRLRMQFYAGAALAQPVWDKLHATAEA 360 Query: 361 TCGERIVMCCGLGMTETSPSALFVADLEVQAGQIGIPTPGMEIKLVPNGDKIEIRYRGPN 420 TCGERIVMCCGLGMTETSPSALFVADLEVQAGQIGIPTPGMEIKLVPNGDKIEIRYRGPN Sbjct: 361 TCGERIVMCCGLGMTETSPSALFVADLEVQAGQIGIPTPGMEIKLVPNGDKIEIRYRGPN 420 Query: 421 VTPGYWRAPEQTAEAFDEEGYFRSGDAVRWLDPAHPDRGFMFDGRVAEDFKLYTGTWVSV 480 VTPGYWRAPEQTAEAFDEEGYFRSGDAVRWLDPAHPDRGFMFDGRVAEDFKLYTGTWVSV Sbjct: 421 VTPGYWRAPEQTAEAFDEEGYFRSGDAVRWLDPAHPDRGFMFDGRVAEDFKLYTGTWVSV 480 Query: 481 GPLRALIAHEGAPYLQDAVITGQDRNEVGMLIVPNIERCRXRISISPADASRADVLNAAP 540 GPLRALIAHEGAPYLQDAVITGQDR E+GMLIVPNIERCR +++ PA+ASRA+VLNAAP Sbjct: 481 GPLRALIAHEGAPYLQDAVITGQDRKEIGMLIVPNIERCR-QLARLPAEASRAEVLNAAP 539 Query: 541 VRDFFQGLLERLQTHATGSSTRVARALVLIDPPSFDRGEITDKGSINQRAVLTHRAALVE 600 VRDFFQG+L+RLQ+HATGSS RV RALVL+DPPSFDRGEITDKGSINQRAVLTHRAALVE Sbjct: 540 VRDFFQGVLDRLQSHATGSSNRVTRALVLVDPPSFDRGEITDKGSINQRAVLTHRAALVE 599 Query: 601 ALYAGTDPAVLTARAALSPAH 621 LYAGTDPAVLT + ALSPAH Sbjct: 600 TLYAGTDPAVLTVQPALSPAH 620 >gi|300311882|ref|YP_003775974.1| long-chain acyl-CoA synthetase [Herbaspirillum seropedicae SmR1] Length = 653 Score = 94.7 bits (234), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 107/470 (22%), Positives = 183/470 (38%), Gaps = 91/470 (19%) Query: 197 DEVERAYAATGPDTIVKFLFTSGSTKMPKAVVNTQRMMCSNLQMIVQTFPFLAEEPPILI 256 D ++ AA PD + L+TSG+T PK V ++ R M + +V+ F L E IL Sbjct: 175 DFFDQEIAAGSPDDVAIILYTSGTTGKPKGVCHSHRAMITTATTLVE-FDHLDENDDILC 233 Query: 257 DWLP------WNHTFGANHNVGIVIYNGGTMYIDEGKPTPQGLATTLANLHEISPTVYFN 310 +LP + ++F H G + P+ T + +L EI PT YF Sbjct: 234 -YLPLAWVGDFLYSFAQQHVAGFCL------------NCPESPNTVMTDLREIGPTYYFA 280 Query: 311 VPVGWEGIAHA----LEKDQALREKFY--------------------------------- 333 P +E I +E L+ K + Sbjct: 281 PPRVYENILTQVMIRIEDAGWLKRKMFHGFMKVARRVGMRILDGKPGVSLLDRLQYGLGS 340 Query: 334 -------------SRLRMQFYAGAALAQPVWDKLHATAEATCGERIVMCCGLGMTETSPS 380 SRLR+ + G A+ ++D + I + GMTET + Sbjct: 341 LLVYGPLKNVLGMSRLRVAYTGGEAIGPDLFDFYRSLG-------INLKQLYGMTETCVT 393 Query: 381 ALFVADLEVQAGQIGIPTPGMEIKLVPNGDKIEIRYRGPNVTPGYWRAPEQTAEAFDEEG 440 +V+ +G P G+E+++ NG E+ R P + Y++ P+ TAEA D G Sbjct: 394 VCMQPSGDVKLDSVGRPMKGVEVRIDENG---EVLVRSPGLMKEYFKRPDATAEAIDANG 450 Query: 441 YFRSGDAVRWLDPAHPDRGFMFDGRVAEDFKLYTGTWVSVGPLRALIAHEGAPYLQDAVI 500 YF +GDA + H R + K+ +G+ + + + P++++AV Sbjct: 451 YFHTGDAGFFDSDGH----LKIIDRAKDVGKMASGSMFAPKYIENKLKF--FPFIKEAVT 504 Query: 501 TGQDRNEVGMLIVPNIERC-----RXRISISPADASRADVLNAAPVRDFFQGLLERLQTH 555 G R + I +++ R ++ S A+ A +R+ + + L Sbjct: 505 FGNGREQCMAFINIDMDAVGNWAERRNLAYSGYTDLAANPAVYALLRECVEKVNADLAAD 564 Query: 556 ATGSSTRVARALVLIDPPSFDRGEITDKGSINQRAVLTHRAALVEALYAG 605 + +++ R L+L D E+T + + + A L+EALYAG Sbjct: 565 PLLADSQIHRFLILHKELDPDDEELTRTRKVRRGFIADKYAVLIEALYAG 614 >gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 566 Score = 68.2 bits (165), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 67/251 (26%), Positives = 102/251 (40%), Gaps = 26/251 (10%) Query: 209 DTIVKFLFTSGSTKMPKAVVNTQRMMCSN-LQMIVQTFPFLAEEPPI----LIDWLPWNH 263 D I +T G+T + K V T R + +N LQ P L PI + LP H Sbjct: 205 DDIAFLQYTGGTTGVAKGAVLTHRNLVANVLQAEEWLTPALKAGKPIDQLVFVCALPLYH 264 Query: 264 TFGANHNVGIVIYNGGTMYIDEGKPTPQGLATTLANLHEISPTVYFNVPVGWEGIAHALE 323 F + G + P P+ + + L + + V + +AL Sbjct: 265 IFALTVCNMLGTREGALNLL---IPNPRDIPGFVKELAKYKVNTFPAV----NTLYNALL 317 Query: 324 KDQALREKFYSRLRMQFYAGAALAQPVWDKLHATAEATCGERIVMCCGLGMTETSP-SAL 382 + +S R G A+ + V DK E G G++ETSP ++ Sbjct: 318 NNADFARLDFSGYRCCVGGGMAVQKSVADKWKKLTGCPIIE------GYGLSETSPIASA 371 Query: 383 FVADLEVQAGQIGIPTPGMEIKL-------VPNGDKIEIRYRGPNVTPGYWRAPEQTAEA 435 ++ G IG+P P E + VP G EI RGP V PGYW+ P++TA+ Sbjct: 372 NPCTIDEYTGTIGLPLPSTEFAILDDDGQPVPLGQPGEIAIRGPQVMPGYWQRPDETAKV 431 Query: 436 FDEEGYFRSGD 446 +G+F++GD Sbjct: 432 MTPDGFFKTGD 442 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,122,612 Number of extensions: 95038 Number of successful extensions: 267 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 269 Number of HSP's successfully gapped: 4 Length of query: 621 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 530 Effective length of database: 1,188,985 Effective search space: 630162050 Effective search space used: 630162050 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)