BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0092.p (194 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313296|ref|YP_003777388.1| type II 3-hydroxyacyl-CoA dehyd... 316 7e-88 gi|300310258|ref|YP_003774350.1| 3-hydroxyacyl-CoA (short-chain)... 209 2e-55 gi|300310506|ref|YP_003774598.1| type II 3-hydroxyacyl-CoA dehyd... 204 5e-54 gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herb... 80 1e-16 gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reduc... 77 1e-15 >gi|300313296|ref|YP_003777388.1| type II 3-hydroxyacyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 254 Score = 316 bits (810), Expect = 7e-88, Method: Compositional matrix adjust. Identities = 171/176 (97%), Positives = 173/176 (98%) Query: 19 RILINCAGIGGAKRMVGKDGSPMPLEDFSRIVNVNLIGTFNVIRLTAARMAAAEPLAEGE 78 RILINCAGIGGAKRMVGKDG PMPLEDFSRIVNVNLIGTFNVIRL AARMAAAE LAEGE Sbjct: 79 RILINCAGIGGAKRMVGKDGKPMPLEDFSRIVNVNLIGTFNVIRLAAARMAAAEALAEGE 138 Query: 79 RGVIVATASVAAFDGQVGQAGYAASKGGITSMTLPLARDLAQHGIRVVTIAPGLFLTPLL 138 RGVIVATASVAAFDGQVGQAGYAASKGGIT+MTLPLARDLAQ+GIRVVTIAPGLFLTPLL Sbjct: 139 RGVIVATASVAAFDGQVGQAGYAASKGGITAMTLPLARDLAQYGIRVVTIAPGLFLTPLL 198 Query: 139 YKLPEEVQQSLASSIPFPKRLGKAEEYAQLALHIATNLSLNGEVIRLDGALRLAPR 194 YKLPEEVQQSLASSIPFPKRLGKAEEYAQLALHIATNLSLNGEVIRLDGALRLAPR Sbjct: 199 YKLPEEVQQSLASSIPFPKRLGKAEEYAQLALHIATNLSLNGEVIRLDGALRLAPR 254 >gi|300310258|ref|YP_003774350.1| 3-hydroxyacyl-CoA (short-chain) dehydrogenase type II oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 254 Score = 209 bits (531), Expect = 2e-55, Method: Compositional matrix adjust. Identities = 100/174 (57%), Positives = 134/174 (77%), Gaps = 1/174 (0%) Query: 21 LINCAGIGGAKRMVGKDGSPMPLEDFSRIVNVNLIGTFNVIRLTAARMAAAEPLAEGERG 80 L+NCAGI ++++GK G L+ F R +N+NL+G+FN+ RL A ++A A GERG Sbjct: 82 LVNCAGIAPGEKLLGKGGVHR-LDSFMRALNINLVGSFNMARLAAQHISAGPADATGERG 140 Query: 81 VIVATASVAAFDGQVGQAGYAASKGGITSMTLPLARDLAQHGIRVVTIAPGLFLTPLLYK 140 VI+ TAS+AAFDGQ+GQ YAASKGG+ SMTLP+AR+LA+HGIRVVTIAPG+ TP++ Sbjct: 141 VIIHTASIAAFDGQMGQVAYAASKGGVVSMTLPMARELARHGIRVVTIAPGIIETPMMLG 200 Query: 141 LPEEVQQSLASSIPFPKRLGKAEEYAQLALHIATNLSLNGEVIRLDGALRLAPR 194 +PEEV +SL+ ++PFP RLGK E+A L +HI N LNGEVIR+DGALR++ + Sbjct: 201 MPEEVLESLSRNVPFPPRLGKPSEFAGLVMHILENRYLNGEVIRMDGALRMSAK 254 >gi|300310506|ref|YP_003774598.1| type II 3-hydroxyacyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 204 bits (518), Expect = 5e-54, Method: Compositional matrix adjust. Identities = 100/176 (56%), Positives = 135/176 (76%), Gaps = 1/176 (0%) Query: 19 RILINCAGIGGAKRMVGKDGSPMPLEDFSRIVNVNLIGTFNVIRLTAARMAAAEPLAEGE 78 R L+NCAGI A+++VGK G P + F + +N+NL+G+FN+ R A MA + + EGE Sbjct: 78 RGLVNCAGIAPAEKLVGKSG-PHTMALFMKTLNINLVGSFNMARFAAEAMARSAAVEEGE 136 Query: 79 RGVIVATASVAAFDGQVGQAGYAASKGGITSMTLPLARDLAQHGIRVVTIAPGLFLTPLL 138 RGVI+ TASVAA+DGQ+GQ YAASKG I +MTLP+AR+L++ GIRV+TIAPG+F TP++ Sbjct: 137 RGVIIHTASVAAYDGQIGQTAYAASKGAIVAMTLPMARELSRSGIRVMTIAPGIFETPMM 196 Query: 139 YKLPEEVQQSLASSIPFPKRLGKAEEYAQLALHIATNLSLNGEVIRLDGALRLAPR 194 +P+EV+ SL ++PFP RLG+A EYAQLA I N+ LNGE IRLDGA+R+ P+ Sbjct: 197 LAMPQEVRDSLGQAVPFPPRLGRAAEYAQLARTIIENVMLNGETIRLDGAIRMQPK 252 >gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 246 Score = 79.7 bits (195), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 56/172 (32%), Positives = 86/172 (50%), Gaps = 25/172 (14%) Query: 20 ILINCAGIGGAKRMVGKDGS--PMPLEDFSRIVNVNLIGTFNVIRLTAARMAAAEPLAEG 77 +L+N AGI +DG M +D+ +++ NL FNV + E + E Sbjct: 84 VLVNNAGIT-------RDGQFRKMSKDDWDAVIDTNLNSLFNVTK------QVIEGMCER 130 Query: 78 ERGVIVATASVAAFDGQVGQAGYAASKGGITSMTLPLARDLAQHGIRVVTIAPGLFLTPL 137 G I+ +SV GQ GQ Y+ +K GI T+ LA+++A G+ V T++PG T + Sbjct: 131 GFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNTVSPGYIGTDM 190 Query: 138 LYKLPEEVQQSLASSIPFPKRLGKAEEYAQLALHIA---------TNLSLNG 180 + + EV + + S IP KRLG+ EE A + IA ++ SLNG Sbjct: 191 VRAIRPEVLEKIVSGIPV-KRLGEPEEIASIVAWIASSEGGYATGSDFSLNG 241 >gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 241 Score = 76.6 bits (187), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 60/177 (33%), Positives = 90/177 (50%), Gaps = 19/177 (10%) Query: 20 ILINCAGIGGAKRMVGKDGSPMPLEDFSRIVNVNLIGTFNVIRLTAARMAAAEPLAEGER 79 I++NCAGI K G+ + E F +++ VNL GT V AAA L R Sbjct: 80 IVVNCAGII-------KRGAELETEVFEQVIAVNLTGTMRVC-------AAARELLRQSR 125 Query: 80 GVIVATASVAAFDGQVGQAGYAASKGGITSMTLPLARDLAQHGIRVVTIAPGLFLTPLLY 139 G IV TAS+ +F G GY+ASKGG+ +T LA A GIRV +APG TPL Sbjct: 126 GCIVNTASMLSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGIRVNAVAPGWIATPLTQ 185 Query: 140 KLPEEVQQS--LASSIPFPKRLGKAEEYAQLALHIATNLS--LNGEVIRLDGALRLA 192 L ++ ++ + + P R G+ E+ Q+ + + + + G ++ +DG +A Sbjct: 186 ALQDDPVRAGPILARTPL-GRWGQPEDVGQVVAFLCSPAASFMTGAIVPVDGGYLVA 241 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 618,181 Number of extensions: 23970 Number of successful extensions: 126 Number of sequences better than 1.0e-08: 21 Number of HSP's gapped: 126 Number of HSP's successfully gapped: 21 Length of query: 194 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 114 Effective length of database: 1,241,070 Effective search space: 141481980 Effective search space used: 141481980 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)