BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0090.p (226 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313298|ref|YP_003777390.1| branched-chain amino acid ABC t... 430 e-122 gi|300313408|ref|YP_003777500.1| branched-chain amino acid ABC t... 182 3e-47 gi|300310681|ref|YP_003774773.1| amino acid ABC transporter peri... 157 9e-40 gi|300312236|ref|YP_003776328.1| amino acid ABC transporter peri... 65 4e-12 gi|300312847|ref|YP_003776939.1| branched-chain amino acid ABC t... 61 1e-10 >gi|300313298|ref|YP_003777390.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 381 Score = 430 bits (1106), Expect = e-122, Method: Compositional matrix adjust. Identities = 214/224 (95%), Positives = 219/224 (97%) Query: 1 MKTKLAIKAGVLAAMLACAGAAQAELTIGVVMSLTGPGSGLGIPAKNGFALWPETIGGEK 60 MKTKLAIKAGVLAAMLACAG A AELTIGVVMSLTGPGSGLGIPAKNGFALWPETI GEK Sbjct: 1 MKTKLAIKAGVLAAMLACAGIAHAELTIGVVMSLTGPGSGLGIPAKNGFALWPETIAGEK 60 Query: 61 VKLIILDDATDPTQASKNARRLVAEDKVDLLIGSAAVPPTLAVADVALESQTVQLAISPI 120 VK+IILDDATDPTQASKNARRLVAEDKVDL+IGSAAVPPTLA+ADVALE+QTVQLAISPI Sbjct: 61 VKMIILDDATDPTQASKNARRLVAEDKVDLIIGSAAVPPTLAIADVALETQTVQLAISPI 120 Query: 121 ETAEGKDAWTFRLPQSTAVMAGGVVEQMKKMGVKTIGFLGYSDSYGENWLKEVQRLAIAA 180 ETAEGKDAWTFRLPQSTAVMAGGVV QMKK+GVKTIGFLGYSDSYGENWLKEVQRLA AA Sbjct: 121 ETAEGKDAWTFRLPQSTAVMAGGVVNQMKKLGVKTIGFLGYSDSYGENWLKEVQRLATAA 180 Query: 181 GMKMGTVERFSRADTSVTAQALRVVSSNPDAVLVVASGSGAAMP 224 GMKMGTVERFSRADTSVTAQALRVVSSNPDAVLVVASGSGAAMP Sbjct: 181 GMKMGTVERFSRADTSVTAQALRVVSSNPDAVLVVASGSGAAMP 224 >gi|300313408|ref|YP_003777500.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 381 Score = 182 bits (461), Expect = 3e-47, Method: Compositional matrix adjust. Identities = 87/206 (42%), Positives = 138/206 (66%), Gaps = 4/206 (1%) Query: 23 QAELTIGVVMSLTGPGSGLGIPAKNGFALWPETIGGEKVKLIILDDATDPTQASKNARRL 82 +A++ +GV +S TGP + LGIP KN AL P TI G KV IILDDA+D TQA KNARR Sbjct: 20 RADINVGVTVSTTGPAASLGIPEKNTIALLPTTIAGHKVNYIILDDASDTTQAVKNARRF 79 Query: 83 VAEDKVDLLIGSAAVPPTLAVADVALESQTVQLAISP----IETAEGKDAWTFRLPQSTA 138 V+E+ VD+++GS+ P ++++ +VA ++QT ++++ +E + K W F+ PQ+ Sbjct: 80 VSENNVDVIVGSSVSPNSISMIEVAADAQTPMISLAAAARIVEPMDAKKKWVFKTPQNDI 139 Query: 139 VMAGGVVEQMKKMGVKTIGFLGYSDSYGENWLKEVQRLAIAAGMKMGTVERFSRADTSVT 198 +M+ + M G + I F+GYS++YGE W +E ++ A +++ ERF+ D+S T Sbjct: 140 MMSLAIARDMVARGYQRIAFIGYSNAYGEGWYEEFKKAAGLKKLELVANERFAPTDSSTT 199 Query: 199 AQALRVVSSNPDAVLVVASGSGAAMP 224 AQAL++V++ PDA+L+ ASG+ A +P Sbjct: 200 AQALKLVAAKPDAILIAASGTPAVVP 225 >gi|300310681|ref|YP_003774773.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 385 Score = 157 bits (396), Expect = 9e-40, Method: Compositional matrix adjust. Identities = 95/231 (41%), Positives = 133/231 (57%), Gaps = 9/231 (3%) Query: 1 MKTKLAIKAGVLAAMLACA-GAAQAELTIGVVMSLTGPGSGLGIPAKNGFALWPETIGGE 59 M K I A VL M + A A A++ +GVV S TGP S +GIP KN L P IG Sbjct: 1 MLHKRLIAAVVLLGMSSLAMHPALAQVKVGVVTSSTGPVSLVGIPQKNTVPLLPTKIGDL 60 Query: 60 KVKLIILDDATDPTQASKNARRLVAEDKVDLLIGSAAVPPTLAVADVALESQTVQLAISP 119 KV+ I LDDA+DPT++ ++L+AE VD +IG + P + V E+ LA P Sbjct: 61 KVEYISLDDASDPTKSVTEIKKLIAEHNVDAIIGPSGSPNAMGVISFVAEAGVPLLA--P 118 Query: 120 IETA------EGKDAWTFRLPQSTAVMAGGVVEQMKKMGVKTIGFLGYSDSYGENWLKEV 173 + TA + W F+ Q+ ++A +V+QM K VKT GF+ +D +GENW + Sbjct: 119 VGTAAVVLPMTEQKKWVFKTTQNDDLIAKALVDQMVKTKVKTAGFIAVNDPFGENWARVF 178 Query: 174 QRLAIAAGMKMGTVERFSRADTSVTAQALRVVSSNPDAVLVVASGSGAAMP 224 LA +K+ ER+ R DTSVTAQ+L++V++NPDAVLV A G+ +P Sbjct: 179 SDLAAKNNIKIVANERYQRTDTSVTAQSLKIVAANPDAVLVAAPGAATVLP 229 >gi|300312236|ref|YP_003776328.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 385 Score = 65.5 bits (158), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 65/229 (28%), Positives = 105/229 (45%), Gaps = 34/229 (14%) Query: 7 IKAGVLAAMLACAGAAQAELTIGVVMSLTGPGSGLGIPAKNGFALWPETI------GGEK 60 I A ++ A L+ A A A++ +GV +LTGP + G+ KNGF L E I G+K Sbjct: 10 IPALLIGATLSQAALA-ADIKLGVAEALTGPAAKYGVAIKNGFTLASEEINAKGGVNGDK 68 Query: 61 VKLIILDDATDPTQASKNARRLVAEDKVDLLIGSAAVPPTLAVADVALESQTVQLAISPI 120 + L+I D+ +A ++L+ +DKV + G A +A ++ V S Sbjct: 69 LALVIEDEQGKKEEAINVFKKLIFQDKVLAVFGPTLSNSAFAADPIANAAKVVVFGTS-- 126 Query: 121 ETAEGKDA---WTFRLPQSTAVMAGGVVEQMKKMGVKTIG------FLGYSDSY---GEN 168 TA+G A +TFR +VM V+ K VK G G D++ G + Sbjct: 127 NTADGITAMGPFTFR----NSVMEADVLPVTVKAAVKHFGIKKVAVIYGNDDAFTKSGYD 182 Query: 169 WLK---EVQRLAIAAGMKMGTVERFSRADTSVTAQALRVVSSNPDAVLV 214 K E Q++ + T E +++ D AQ ++ + NPDA++ Sbjct: 183 VFKATLEHQKIPVT------TTEAYAKGDVDFKAQLTKIKAGNPDAIVC 225 >gi|300312847|ref|YP_003776939.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 383 Score = 60.8 bits (146), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 60/232 (25%), Positives = 108/232 (46%), Gaps = 17/232 (7%) Query: 10 GVLAAMLACAGAAQ-----AELTIGVVMSLTGPGSGLG----IPAKNGFALWPET--IGG 58 G+ ++LA AA+ E+ +G +GP S LG + A++ FA+ + G Sbjct: 14 GLAMSLLAVGAAAEDGVSATEVVLGQSAPFSGPASDLGRDFLVGAQSYFAMVNAKGGVHG 73 Query: 59 EKVKLIILDDATDPTQASKNARRLVAEDKVDLLIGSAAVPPTLAVADVALESQTVQLAIS 118 +V+L+ LDD +P + + N ++L+ DKV L G + TLA A A+ V L + Sbjct: 74 RRVRLLSLDDGYEPERTAANTQQLIWRDKVFALFGYSGTATTLA-ALPAIREANVPL-FA 131 Query: 119 PIETA----EGKDAWTFRLPQSTAVMAGGVVEQMKKMGVKTIGFLGYSDSYGENWLKEVQ 174 P+ A E + F + S A ++ Q++ G + + +D G++ L++++ Sbjct: 132 PVSGALALREPFNRLIFHVRASYAQETDFLLRQLQGTGRRKVAVFYQNDELGKSMLEQLR 191 Query: 175 RLAIAAGMKMGTVERFSRADTSVTAQALRVVSSNPDAVLVVASGSGAAMPIS 226 A+ G+ + R +V A ++ PD V+ V S AA I+ Sbjct: 192 LRALVYGLTLVGTAAVERNSDAVEGAAQALLKVKPDVVIQVVSYRPAAALIA 243 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.129 0.358 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 595,212 Number of extensions: 23347 Number of successful extensions: 128 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 127 Number of HSP's successfully gapped: 11 Length of query: 226 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 144 Effective length of database: 1,231,600 Effective search space: 177350400 Effective search space used: 177350400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)