BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0087.p (327 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313300|ref|YP_003777392.1| branched-chain amino acid ABC t... 582 e-167 gi|300313405|ref|YP_003777497.1| branched-chain amino acid ABC t... 338 4e-94 gi|300310683|ref|YP_003774775.1| amino acid ABC transporter comp... 274 6e-75 gi|300311115|ref|YP_003775207.1| branched-chain amino acid ABC t... 121 6e-29 gi|300309520|ref|YP_003773612.1| branched-chain amino acid ABC t... 101 7e-23 >gi|300313300|ref|YP_003777392.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 597 Score = 582 bits (1500), Expect = e-167, Method: Compositional matrix adjust. Identities = 297/302 (98%), Positives = 300/302 (99%) Query: 1 MMMNKRLPLYALLVVLALFPILPTPQFWVIQANYIGLYALVAIGLVLLTGIGGMTSFGQA 60 M MNKR+PLYALLVVLALFP+LPTPQFWVIQANYIGLYALVAIGLVLLTGIGGMTSFGQA Sbjct: 1 MSMNKRIPLYALLVVLALFPLLPTPQFWVIQANYIGLYALVAIGLVLLTGIGGMTSFGQA 60 Query: 61 AFVGMGAYTTSYLTTVHGVSPWLTLPIGLFITGVSAYVLGRITLRMSGHYLPLATIAWGI 120 AFVGMGAYTTSYLTTVHGVSPWLTLP+GLFITGVSAYVLGRITLRMSGHYLPLATIAWGI Sbjct: 61 AFVGMGAYTTSYLTTVHGVSPWLTLPVGLFITGVSAYVLGRITLRMSGHYLPLATIAWGI 120 Query: 121 SLYFLFGKVDFLGKYDGISGIPALQLAGLDLAKERHLYYLIWVIVLAAAWVSLNLLDSRA 180 SLYFLFGKVDFLGKYDGISGIPALQLAGLDLAKERHLYYLIW IVLAAAWVSLNLLDSRA Sbjct: 121 SLYFLFGKVDFLGKYDGISGIPALQLAGLDLAKERHLYYLIWAIVLAAAWVSLNLLDSRA 180 Query: 181 GRAIRALKGGRSMAEAMGVDTGRYKILIFVFAALLASVSGWLFAHMQRTVNPSPFSLAMG 240 GRAIRALKGGRSMAEAMGVDTGRYKILIFVFAALLASVSGWLFAHMQRTVNPSPFSLAMG Sbjct: 181 GRAIRALKGGRSMAEAMGVDTGRYKILIFVFAALLASVSGWLFAHMQRTVNPSPFSLAMG 240 Query: 241 IEYLFIVVVGGIAHIWGALLGAGVLKLLSDQLQVLLPALLGDGGSYESIVFGLILILLLK 300 IEYLFIVVVGGIAHIWGALLGAGVLKLLSDQLQVLLPALLGDGGSYESIVFGLILILLLK Sbjct: 241 IEYLFIVVVGGIAHIWGALLGAGVLKLLSDQLQVLLPALLGDGGSYESIVFGLILILLLK 300 Query: 301 YA 302 YA Sbjct: 301 YA 302 >gi|300313405|ref|YP_003777497.1| branched-chain amino acid ABC transporter ATP-binding protein/permease [Herbaspirillum seropedicae SmR1] Length = 596 Score = 338 bits (867), Expect = 4e-94, Method: Compositional matrix adjust. Identities = 170/297 (57%), Positives = 230/297 (77%), Gaps = 1/297 (0%) Query: 7 LPLYALLVVLALFPILPTPQFWVIQANYIGLYALVAIGLVLLTGIGGMTSFGQAAFVGMG 66 LP +VLAL P L P FWV +Y+GLYA+VA+GLVLLTG+ G+ SFGQAAFVGMG Sbjct: 11 LPALFFAIVLAL-PQLGLPPFWVTLLSYVGLYAIVALGLVLLTGVAGLISFGQAAFVGMG 69 Query: 67 AYTTSYLTTVHGVSPWLTLPIGLFITGVSAYVLGRITLRMSGHYLPLATIAWGISLYFLF 126 AYTT+YLTT G+SPW+ L G+ +T SA+++G +T+R+SGHYLPL+TIAWG+SLY+LF Sbjct: 70 AYTTAYLTTQFGLSPWIGLLAGIALTACSAFLIGSLTMRISGHYLPLSTIAWGLSLYYLF 129 Query: 127 GKVDFLGKYDGISGIPALQLAGLDLAKERHLYYLIWVIVLAAAWVSLNLLDSRAGRAIRA 186 G ++ LGK+DGI+GIP + + ++ +Y LIWV+++ A +N LDSRAGRA+RA Sbjct: 130 GNLEMLGKHDGINGIPVITIGSVEFGNGASMYLLIWVVLILALVSVVNFLDSRAGRAVRA 189 Query: 187 LKGGRSMAEAMGVDTGRYKILIFVFAALLASVSGWLFAHMQRTVNPSPFSLAMGIEYLFI 246 L+ G M EAMGVDT R KI+IFV+AA+LA +SGWL+AH+Q+ VNP+PF L GI+YL + Sbjct: 190 LRFGTVMPEAMGVDTARLKIVIFVYAAVLAGISGWLYAHLQQAVNPTPFGLKYGIDYLLM 249 Query: 247 VVVGGIAHIWGALLGAGVLKLLSDQLQVLLPALLGDGGSYESIVFGLILILLLKYAR 303 VVVGG H+ GA+LGAG+L +LS+ LQ LP +LG GG++E+IVFG+ L++ L++A Sbjct: 250 VVVGGAGHVAGAILGAGLLAVLSNFLQEWLPGVLGTGGNFEAIVFGIALVIFLQFAN 306 >gi|300310683|ref|YP_003774775.1| amino acid ABC transporter composite ATP-binding protein/permease [Herbaspirillum seropedicae SmR1] Length = 627 Score = 274 bits (701), Expect = 6e-75, Method: Compositional matrix adjust. Identities = 153/290 (52%), Positives = 212/290 (73%), Gaps = 9/290 (3%) Query: 27 FWVIQANYIGLYALVAIGLVLLTGIGGMTSFGQAAFVGMGAYTTSYLTT---------VH 77 F++ N IGL +LV +GLVLLTG+ G+TSFGQAAFVG+GAY ++ L+ + Sbjct: 31 FYMTLLNNIGLASLVVLGLVLLTGVAGLTSFGQAAFVGLGAYFSAALSAKVLPLPEALLW 90 Query: 78 GVSPWLTLPIGLFITGVSAYVLGRITLRMSGHYLPLATIAWGISLYFLFGKVDFLGKYDG 137 SPW L + L +T + A+ +G +TL++SGHYLPL TIAWGISLYF+FG ++ +G G Sbjct: 91 LQSPWPALAVALVLTALLAFAVGSVTLKLSGHYLPLGTIAWGISLYFIFGTLESMGGQTG 150 Query: 138 ISGIPALQLAGLDLAKERHLYYLIWVIVLAAAWVSLNLLDSRAGRAIRALKGGRSMAEAM 197 +SGIP++ G+ L +R ++ LIWV VL NLLDSR GRAIRALKGG MAEAM Sbjct: 151 MSGIPSIAFLGVVLDSDRAMFGLIWVAVLLGVAALRNLLDSREGRAIRALKGGIVMAEAM 210 Query: 198 GVDTGRYKILIFVFAALLASVSGWLFAHMQRTVNPSPFSLAMGIEYLFIVVVGGIAHIWG 257 GV+T R +I++FV AA+LA++SGWL+ HMQR VNPSPF L GI+YLF+ V+GG +H+WG Sbjct: 211 GVNTARMRIVVFVIAAVLAALSGWLYVHMQRFVNPSPFGLGFGIDYLFMAVLGGASHVWG 270 Query: 258 ALLGAGVLKLLSDQLQVLLPALLGDGGSYESIVFGLILILLLKYARFFGW 307 A+LG+ ++ +L + LQ LLP +LG G++E+IVFGL++++LL+ +R W Sbjct: 271 AVLGSSLIVVLKEWLQGLLPVVLGRSGNFETIVFGLLIVVLLQRSREGIW 320 >gi|300311115|ref|YP_003775207.1| branched-chain amino acid ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 655 Score = 121 bits (304), Expect = 6e-29, Method: Compositional matrix adjust. Identities = 87/283 (30%), Positives = 155/283 (54%), Gaps = 13/283 (4%) Query: 3 MNKRLPLYALLVVLAL--FPILPTPQFWVIQANYIGLYALVAIGLVLLTGIGGMTSFGQA 60 MNK++ L +LL ++AL P++ +++ I +YA++ GL ++ G G S G A Sbjct: 1 MNKKILLLSLLGIVALAGIPMVVHNPYYLHLIETILIYAILLFGLDIVVGYTGQVSLGHA 60 Query: 61 AFVGMGAYTTSYLTTVHGVSPWLTLPIGLFITGVSAYVLGRITLRMSGHYLPLATIAWGI 120 G+G+Y L G+ ++T+P + +T V +L LR++G YL + T+A+G Sbjct: 61 GLFGIGSYAAGVLFFKLGMPIFVTVPAAIAVTAVFGAILALPALRVTGPYLAMVTLAFGT 120 Query: 121 SLYFLFGKVDFLGKYDGISGIPALQ-LAGLDLAKERHLYYLIWVIVLAAAWVSLNLLDSR 179 + L ++ FL +G GI + + G D E +YL+ V+++ + V +L S Sbjct: 121 IIQILINEMTFL--TEGPLGIKLQKPMIGGDKMSEVQFFYLVAVLMVLSMLVVHRILKSN 178 Query: 180 AGRAIRALKGGRSMAEAMGVDTGRYKILIFVFAALLASVSGWLFAHMQRTVNPSPFSLAM 239 GRA +AL+ ++ MGV RYK+ F+ +A LA +SG L+A+ + ++P+ ++ + Sbjct: 179 LGRAFQALRDSPIASDCMGVSVYRYKVYAFIISAALAGLSGALYAYSEEYISPNTYNFEL 238 Query: 240 GIEYLFIVVVGGIAHIWGALLGAGVLKLLSDQLQVLLPALLGD 282 I +L V++GG G+L+GA ++ V+LP+LL D Sbjct: 239 TIMFLLAVIMGGRKSRIGSLIGALIV--------VMLPSLLSD 273 >gi|300309520|ref|YP_003773612.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 404 Score = 101 bits (252), Expect = 7e-23, Method: Compositional matrix adjust. Identities = 94/333 (28%), Positives = 152/333 (45%), Gaps = 61/333 (18%) Query: 28 WVIQANYIGLYALVAIGLVLLTGIGGMTSFGQAAFVGMGAYT-------------TSYLT 74 WV + LY ++A+GL ++ G G+ G AF +GAY+ S++ Sbjct: 40 WVRIMDVALLYIMLALGLNVVVGFAGLLDLGYIAFYAIGAYSAGLLASPQFAAVIESFVN 99 Query: 75 TVHGV-------------------SPWLTLPIGLFITGVSAYVLGRITLRMSGHYLPLAT 115 T V S WL +PI F+ + +LG TL++ G YL + T Sbjct: 100 TYPSVGNFLVWLCGPEIVQNGIHLSLWLIVPISAFLAALFGALLGAPTLKLRGDYLAIVT 159 Query: 116 IAWG----ISLYFLFGKVDFLGKYDGISGIPALQLAGLDLAKE----------------- 154 + +G I + L V+ GI+ I +++ G+ LA E Sbjct: 160 LGFGEIIRIFMNNLNAPVNITNGPQGINLIDPIKVFGVSLAGEPGSGSMVKVFGMSMPSV 219 Query: 155 RHLYYLIWVIVLAAAWVSLNLLDSRAGRAIRALKGGRSMAEAMGVDTGRYKILIFVFAAL 214 Y+L ++ + + S+ L DSR GRA A++ A+AMG++T K+L F A Sbjct: 220 NAYYFLFLLLCIGVIFFSVRLQDSRLGRAWVAIREDEIAAKAMGINTRNVKLLAFAMGAS 279 Query: 215 LASVSGWLFAHMQRTVNPSPFSLAMGIEYLFIVVVGGIAHIWGALLGAGVLKLLSDQLQ- 273 V+G +F Q V+P FSL I L +VV+GGI HI G +LG +L L + L+ Sbjct: 280 FGGVAGAMFGAFQGFVSPESFSLTESIAVLAMVVLGGIGHIPGVVLGGVILAALPEVLRH 339 Query: 274 VLLP---ALLG----DGGSYESIVFGLILILLL 299 V+ P A+ G D +++GL +++++ Sbjct: 340 VVEPVQMAIFGKVWIDAEVLRQLLYGLAMVVIM 372 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.145 0.449 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,029,833 Number of extensions: 44433 Number of successful extensions: 282 Number of sequences better than 1.0e-08: 30 Number of HSP's gapped: 245 Number of HSP's successfully gapped: 31 Length of query: 327 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 242 Effective length of database: 1,217,395 Effective search space: 294609590 Effective search space used: 294609590 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 131 (55.1 bits)