BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0083.p (608 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbasp... 1091 0.0 gi|300309596|ref|YP_003773688.1| acyl-CoA dehydrogenase [Herbasp... 529 e-151 gi|300310835|ref|YP_003774927.1| acyl-CoA dehydrogenase [Herbasp... 387 e-108 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 132 8e-32 gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbasp... 122 1e-28 >gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 591 Score = 1091 bits (2821), Expect = 0.0, Method: Compositional matrix adjust. Identities = 549/607 (90%), Positives = 566/607 (93%), Gaps = 17/607 (2%) Query: 1 MTMHYTPPLRDFAFVLNEVLQAPQVLRALPAHAEIDGELMMQVLEEAGRFAADVVAPLNG 60 MTMHY PPLRDFAFVL+EVLQAPQVLRALPAHAEIDG+LMMQVLEEAGRFAADVVAPLNG Sbjct: 1 MTMHYAPPLRDFAFVLDEVLQAPQVLRALPAHAEIDGDLMMQVLEEAGRFAADVVAPLNG 60 Query: 61 IGDREGCRLENGTVTTPPGFERAYAQFCAGGWPALACAPEHGGQGLPHVLDCALYEMLSA 120 IGDREGCR +NGTVTTPPGFERAYAQFCAGGWPALACAPEHGGQGLPHVLDCALYEMLSA Sbjct: 61 IGDREGCRFKNGTVTTPPGFERAYAQFCAGGWPALACAPEHGGQGLPHVLDCALYEMLSA 120 Query: 121 ANHGWTMYPGLLHGAYACLAAHGSPELQARYLEKIASGQWLATMCLTEAQAGSDLGQLRT 180 ANHGWTMYPGLLHGAYACLAAHGS LQARYL+KIASG+WLATMCLTEAQAGSDLGQLRT Sbjct: 121 ANHGWTMYPGLLHGAYACLAAHGSAALQARYLDKIASGEWLATMCLTEAQAGSDLGQLRT 180 Query: 181 RAVPQEDGSYRLTGDKIFISGGEHDLTPNIIHLVLARLPDALPGSKGLSLFLAPRVLDDG 240 RAVPQ+DGSYRL+GDKIFISGGEHDLTPNI+HLVLARLPDA GSKGLSLFLAPRVLDDG Sbjct: 181 RAVPQDDGSYRLSGDKIFISGGEHDLTPNIVHLVLARLPDAPAGSKGLSLFLAPRVLDDG 240 Query: 241 SRNGVHCTGIEHKMGIHGSATCSLHFEQAQAWLVGEAGRGLAAMFVMMNAARLHVGAQGL 300 SRNGVHCTGIEHKMGIHGSATCSLHFEQAQAWLVGEAGRGLAAMFVMMNAARLHVGAQGL Sbjct: 241 SRNGVHCTGIEHKMGIHGSATCSLHFEQAQAWLVGEAGRGLAAMFVMMNAARLHVGAQGL 300 Query: 301 GLAEAAWQRASAYARERRQSRAPGSSGNDLIVRHPAVQKLLMEQRCRIEGARLLTCWAGL 360 GLAEAA+QRASAYARERRQSRAPGSSGNDLIV HPAVQKLLMEQRCRIEGARLLTCWAGL Sbjct: 301 GLAEAAYQRASAYARERRQSRAPGSSGNDLIVHHPAVQKLLMEQRCRIEGARLLTCWAGL 360 Query: 361 LLDLAEHDPDAAQRRPGQGDYIQPDRHRRQRRRHHERLEFITPVIKAFLTDQGFQCASRA 420 LLDLAEHDP+AA R RHHERLEFITPVIKAFLTDQGFQCASRA Sbjct: 361 LLDLAEHDPEAA-----------------LRSRHHERLEFITPVIKAFLTDQGFQCASRA 403 Query: 421 LQVFGGHGYVVETGIEQFMRDARITMIYEGTNEIQAIDFLMRKVLGDEGKRLDDFLTLVR 480 LQVFGGHGYVVETGIEQFMRDARITMIYEGTNEIQAIDFLMRKVLGDEG+RLDDFL LVR Sbjct: 404 LQVFGGHGYVVETGIEQFMRDARITMIYEGTNEIQAIDFLMRKVLGDEGRRLDDFLGLVR 463 Query: 481 ADIEDGTLLGEQARLLANAVDKLSGLATRIASAAVQSPSLPYYVADEMLRLTGHVALGWM 540 ADI + + LGEQARLL AVDKL A+RIA+AA Q+P+LPYYVADEMLRL GHVALGWM Sbjct: 464 ADIAEASGLGEQARLLGEAVDKLRIFASRIAAAAAQAPALPYYVADEMLRLCGHVALGWM 523 Query: 541 WLRAGRRALSAMASDPAWYGGKRDAACYHFAFVFAEVHQLSSVIEGCLATGLPPLPTDLD 600 WLRAGRRALSAMA DP WYGGKRDAACYHFAFVFAEV QLSSVIE CLATGLPPLPTDLD Sbjct: 524 WLRAGRRALSAMAQDPLWYGGKRDAACYHFAFVFAEVQQLSSVIEACLATGLPPLPTDLD 583 Query: 601 HHAAQEA 607 HHAAQEA Sbjct: 584 HHAAQEA 590 >gi|300309596|ref|YP_003773688.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 596 Score = 529 bits (1363), Expect = e-151, Method: Compositional matrix adjust. Identities = 276/592 (46%), Positives = 369/592 (62%), Gaps = 32/592 (5%) Query: 4 HYTPPLRDFAFVLNEVLQAPQVLRALPAHAEIDGELMMQVLEEAGRFAADVVAPLNGIGD 63 Y PLRD FVL+E+L +V + LP HA+ + EL+ QVLEE G+FA+ V+ PLN GD Sbjct: 3 QYNAPLRDMQFVLHELLNVEEVFKELPPHADTNRELVDQVLEEGGKFASQVLFPLNHSGD 62 Query: 64 REGCRL--ENGTVTTPPGFERAYAQFCAGGWPALACAPEHGGQGLPHVLDCALYEMLSAA 121 REGC E+ TVTTP GF++AY Q+ GW AL+C PE GGQGLP ++ + YEML+++ Sbjct: 63 REGCHYDRESKTVTTPKGFKQAYQQYVEAGWAALSCDPEFGGQGLPLTINNSFYEMLNSS 122 Query: 122 NHGWTMYPGLLHGAYACLAAHGSPELQARYLEKIASGQWLATMCLTEAQAGSDLGQLRTR 181 N WTMYPGL HGAY CL AHGS E + YL K+ SG+W TMCLTEA G+DLG LR++ Sbjct: 123 NQAWTMYPGLSHGAYECLHAHGSEEQKQLYLPKLVSGEWTGTMCLTEAHCGTDLGLLRSK 182 Query: 182 AVPQEDGSYRLTGDKIFISGGEHDLTPNIIHLVLARLPDALPGSKGLSLFLAPRVLDD-- 239 A PQ DGSY++TG+KIFIS GE+D++ NIIHLVLARLPDA GSKG+SLFL P+ L + Sbjct: 183 AEPQADGSYKITGNKIFISAGENDVSANIIHLVLARLPDAPEGSKGISLFLVPKFLPNAD 242 Query: 240 ---GSRNGVHCTGIEHKMGIHGSATCSLHFEQAQAWLVGEAGRGLAAMFVMMNAARLHVG 296 G+RN + C IE KMGIHG+ATC ++ + A WL+G+ +GL AMFV MNAARL VG Sbjct: 243 GSLGARNPIFCGAIEEKMGIHGNATCQMNLDGATGWLIGQPHKGLQAMFVFMNAARLGVG 302 Query: 297 AQGLGLAEAAWQRASAYARERRQSRA-----PGSSGNDLIVRHPAVQKLLMEQRCRIEGA 351 QGLGL E A+Q A YA++R Q R+ D I+ HP V+++L+ + EG Sbjct: 303 MQGLGLTEVAYQNALVYAKDRLQMRSLSGIKAADKPADPIIVHPDVRRMLLTAKAFAEGG 362 Query: 352 RLLTCWAGLLLDLAEHDPDAAQRRPGQGDYIQPDRHRRQRRRHHERLEFITPVIKAFLTD 411 R T + L LD PD + R+ + + +TPV+KAFLTD Sbjct: 363 RAFTSYVALELDKELTHPD-----------------EQVRKDSADIVALLTPVVKAFLTD 405 Query: 412 QGFQCASRALQVFGGHGYVVETGIEQFMRDARITMIYEGTNEIQAIDFLMRKVLGDEGKR 471 + S LQV+GGHGY+ E G+EQ++RDARI MIYEGTN IQ++D L RK+L D G + Sbjct: 406 NAWMATSECLQVYGGHGYIAEWGMEQYVRDARINMIYEGTNTIQSLDLLGRKILMDNGAK 465 Query: 472 LDDFLTLVRADIED-GT--LLGEQARLLANAVDKLSGLATRIASAAVQSPSLPYYVADEM 528 L F ++A +E+ GT + E LA+ DK++ L I + + A Sbjct: 466 LKKFGAQIQAFVEEHGTDESMSEFITPLADIGDKVTKLTMEIGMKSFANQDEAGAAAVPY 525 Query: 529 LRLTGHVALGWMWLRAGRRALSAMASDPAWYGGKRDAACYHFAFVFAEVHQL 580 LR+ GH+ +++ R + AL S ++Y K A ++FA + E L Sbjct: 526 LRVVGHLVFSYLFARMAKIALEKQDSGDSFYAAKLATARFYFARLLPETAML 577 >gi|300310835|ref|YP_003774927.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 387 bits (993), Expect = e-108, Method: Compositional matrix adjust. Identities = 231/557 (41%), Positives = 305/557 (54%), Gaps = 46/557 (8%) Query: 3 MHYTPPLRDFAFVLNEVLQAPQVLRALPAHAEIDGELMMQVLEEAGRFAADVVAPLNGIG 62 M YT P++D FV+NE L + ALP + E +LEE +F ++VVAPLN Sbjct: 1 MSYTAPVKDMLFVMNE-LAGLAAVNALPGCEDATPETAEAILEENAKFCSEVVAPLNHSA 59 Query: 63 DREGCRLENGTVTTPPGFERAYAQFCAGGWPALACAPEHGGQGLPHVLDCALYEMLSAAN 122 D+E +G VTT PGF+ A+ QF GW + E GGQGLP +L EML++AN Sbjct: 60 DKEPSSWADGKVTTSPGFKEAFRQFGEAGWQGVQHPVEFGGQGLPKLLATPCIEMLNSAN 119 Query: 123 HGWTMYPGLLHGAYACLAAHGSPELQARYLEKIASGQWLATMCLTEAQAGSDLGQLRTRA 182 + + P L G L G+ + + ++ SG+W TM LTE QAGSDL +RTRA Sbjct: 120 LSFALCPLLTDGTIEALMTAGTQQQKETFIANFISGKWTGTMNLTEPQAGSDLALVRTRA 179 Query: 183 VPQEDGSYRLTGDKIFISGGEHDLTPNIIHLVLARLPDALPGSKGLSLFLAPRVLDD--- 239 VP+ DG+Y+L G KIFI+ GEHD+ NI+HLVLAR P+A G KG+SLF+ P+ L + Sbjct: 180 VPEGDGTYKLFGTKIFITYGEHDMAENIVHLVLARTPNAPEGVKGISLFIVPKFLVNADG 239 Query: 240 --GSRNGVHCTGIEHKMGIHGSATCSLHFEQ---AQAWLVGEAGRGLAAMFVMMNAARLH 294 G+RN VHC IEHK+GI S T L F A LVGE RGL MF+MMNAAR Sbjct: 240 SLGARNDVHCVSIEHKLGIKASPTAVLQFGDHGGAIGTLVGEENRGLEYMFIMMNAARFA 299 Query: 295 VGAQGLGLAEAAWQRASAYARERRQSR-APGSSGNDLIVRHPAVQKLLMEQRCRIEGARL 353 VG QGL ++E A+Q+A AYAR+R QSR GS G I+ P V+++LM R +IE AR Sbjct: 300 VGIQGLSVSERAYQKAVAYARDRIQSRDLAGSPGPVAIIHQPDVRRMLMSMRAQIEAARA 359 Query: 354 LTCWAGLLLDLAEHDPDAAQRRPGQGDYIQPDRHRRQRRRHHERLEFITPVIKAFLTDQG 413 L+ A D A D A R+ Q Y E++ P++K ++T+ Sbjct: 360 LSYVAAATSDAAHFSDDEATRKSNQAFY-----------------EYLVPIVKGWITEMS 402 Query: 414 FQCASRALQVFGGHGYVVETGIEQFMRDARITMIYEGTNEIQAIDFLMRKVLGDEGKRLD 473 S +QV GG G++ ETG Q+ RDARI IYEGT IQA D + RK D G Sbjct: 403 IDVTSTGVQVHGGMGFIEETGAAQYYRDARILPIYEGTTAIQANDLVGRKTARDGGAVAK 462 Query: 474 DFLTLVRADIED----------GTLLGEQARLLANAVDK-LSGLATRIASAAVQSPSLPY 522 L VR + + LGE A L VD ++ + + I AV + S+PY Sbjct: 463 GILAQVRKTVSELQGDAQLQPIAARLGEAADALEQVVDYVVANVKSDI--KAVFAGSVPY 520 Query: 523 YVADEMLRLTGHVALGW 539 L++ G V GW Sbjct: 521 ------LKMAGVVLGGW 531 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 132 bits (332), Expect = 8e-32, Method: Compositional matrix adjust. Identities = 115/367 (31%), Positives = 167/367 (45%), Gaps = 59/367 (16%) Query: 97 CAPEH-GGQGLPHV-LDCALYEMLSAANHGWTMYPGLLHGAYAC--LAAHGSPELQARYL 152 C PE GG G+ ++ L AL E+ AA G T + + AC +GSP + ++L Sbjct: 52 CVPEQWGGAGMDYMSLVLALEEI--AAGDGATSTIVSVQNSLACGITEKYGSPTQKEQWL 109 Query: 153 EKIASGQWLATMCLTEAQAGSDLGQLRTRAVPQEDG-SYRLTGDKIFISGGEHDLTPNII 211 + +A G+ L CLTE GSD +RTRA + DG + L G K FI+ G+H + Sbjct: 110 KPLARGEMLGCFCLTEPHTGSDAAAIRTRA--ERDGDDFILNGTKQFITSGKH----AGV 163 Query: 212 HLVLARLPDALPGSKGLSLFLAPRVLDDGSRNGVHCTGIEHKMGIHGSATCSLHFEQAQ- 270 +V A + D G KG+S FL P G E KMG H S T + + + Sbjct: 164 AIVFA-VTDRSAGKKGISCFLVP-----CDTPGFVVGRSEEKMGQHASDTVQIMLDNCRV 217 Query: 271 --AWLVGEAGRGLAAMFVMMNAARLHVGAQGLGLAEAAWQRASAYARERRQSRAPGSSGN 328 L+G+ G G + A R+ + AQ +G+A AA++ A +YAR+R P Sbjct: 218 PATALLGKEGEGYRIALSNLEAGRIGIAAQSVGMARAAFEAAVSYARQRESFGVP----- 272 Query: 329 DLIVRHPAVQKLLMEQRCRIEGARLLTCWAGLLLDLAEHDPDAAQRRPGQGDYIQPDRHR 388 I+ H AV L + ++ ARL+ A L D Q RP + Sbjct: 273 --IIEHQAVNFRLADMNTLLDAARLMVWRAAQLKD---------QGRPCLKE-------- 313 Query: 389 RQRRRHHERLEFITPVIKAFLTDQGFQCASRALQVFGGHGYVVETGIEQFMRDARITMIY 448 + K F ++ + AS A+Q+ GG GY + +E+ RD RI IY Sbjct: 314 -------------ASMAKMFASEAAEKIASDAIQIHGGVGYTSDFPVERIYRDVRICQIY 360 Query: 449 EGTNEIQ 455 EG N+IQ Sbjct: 361 EGANDIQ 367 >gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 122 bits (305), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 114/389 (29%), Positives = 179/389 (46%), Gaps = 56/389 (14%) Query: 91 GWPALACAPEHGGQGLPHVLDCALYEMLSAANHGWTMYPGLLHGAYACLAAHGSPELQAR 150 G+ L GG GL + ++E L+ A Y + + +A+ G P L+A+ Sbjct: 49 GFCGLYTPEADGGLGLSRLDATVVFEELARACTSTAAYLTIHNMVSWMIASWGQPSLKAQ 108 Query: 151 YLEKIASGQWLATMCLTEAQAGSDLGQLRTRAVPQEDGSYRLTGDKIFISG-GEHDLTPN 209 + E++A+G+ + + CLTE +GSD L+T A EDG Y L G K FISG G D Sbjct: 109 WCEQLAAGRKIGSYCLTEPGSGSDAASLKTTA-RLEDGHYVLNGSKAFISGAGSTD---- 163 Query: 210 IIHLVLARLPDALPGSKGLSLFLAPRVLDDGSRNGVHCTGIEHKMGIHGSATCSLHFEQA 269 + +V+AR G++G+S + P G+ GV E KMG + T ++ F+ Sbjct: 164 -VLVVMARTGG--EGARGVSAIVVP-----GNAPGVSYGKKESKMGWNSQPTRTISFDNV 215 Query: 270 QA---WLVGEAGRGLAAMFVMMNAARLHVGAQGLGLAEAAWQRASAYARERRQSRAPGSS 326 + L+G+ G+G ++ R+++ +G A+AA A AY +ER+Q P Sbjct: 216 KVPADHLLGQEGQGFIFAMKGLDGGRINIATCSVGAAQAALDAAHAYMKERKQFGRP--- 272 Query: 327 GNDLIVRHPAVQKLLMEQRCRIEGARLLTCWAGLLLDLAEHDPDAAQRRPGQGDYIQPDR 386 I A+Q L + + + AR + A LD P+A Sbjct: 273 ----IADFQALQFKLADMQTELVAARQMVRLAATKLDAGS--PEATT------------- 313 Query: 387 HRRQRRRHHERLEFITPVIKAFLTDQGFQCASRALQVFGGHGYVVETGIEQFMRDARITM 446 + K TD GF+ + ALQ+ GG+GY+ E +E++ RD R+ Sbjct: 314 --------------YCAMAKRLATDLGFRVCNEALQLHGGYGYIREYPLERYFRDVRVHQ 359 Query: 447 IYEGTNEIQAIDFLMRKVLGDEGKRLDDF 475 I EGTNEI + + RK+L + LDD Sbjct: 360 ILEGTNEIMRV-IISRKLL--DRDNLDDI 385 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.137 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,099,871 Number of extensions: 95606 Number of successful extensions: 314 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 293 Number of HSP's successfully gapped: 10 Length of query: 608 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 517 Effective length of database: 1,188,985 Effective search space: 614705245 Effective search space used: 614705245 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)