BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0022.p (119 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313351|ref|YP_003777443.1| glutamine amidotransferase [Her... 225 6e-61 gi|300313488|ref|YP_003777580.1| glutamine amidotransferase [Her... 72 8e-15 >gi|300313351|ref|YP_003777443.1| glutamine amidotransferase [Herbaspirillum seropedicae SmR1] Length = 212 Score = 225 bits (574), Expect = 6e-61, Method: Compositional matrix adjust. Identities = 110/112 (98%), Positives = 112/112 (100%) Query: 1 MNKIVVVDYGMGNLRSVAQALRHVAPEADVRISGEVADIRAADRVVLPGQGAMPDCMRSL 60 MNKIVVVDYGMGNLRSVAQALRHVAPEADVRISGEVADIRAADRVVLPGQGAMPDCMRSL Sbjct: 1 MNKIVVVDYGMGNLRSVAQALRHVAPEADVRISGEVADIRAADRVVLPGQGAMPDCMRSL 60 Query: 61 RESGVQDAVLEASRTKPLFGVCVGEQMLFDWSEEGDTPGLGLLPGKVVRFEL 112 RESGVQDAV+EASRTKPLFGVCVGEQMLFDWSEEGDTPGLGLLPGKVVRF+L Sbjct: 61 RESGVQDAVIEASRTKPLFGVCVGEQMLFDWSEEGDTPGLGLLPGKVVRFDL 112 >gi|300313488|ref|YP_003777580.1| glutamine amidotransferase [Herbaspirillum seropedicae SmR1] Length = 202 Score = 72.4 bits (176), Expect = 8e-15, Method: Compositional matrix adjust. Identities = 42/110 (38%), Positives = 66/110 (60%), Gaps = 5/110 (4%) Query: 4 IVVVDYGMGNLRSVAQALRHVAPEADVRISGEVADIRAADRVVLPGQGAMPDCMRSLRES 63 I +V+YGMGNL S+ + + +A RI + +R A+++VLPG GA M + E+ Sbjct: 2 ITIVNYGMGNLGSLLNMFKRIGVKA--RIESDPEAVRDAEKLVLPGVGAFDAAMHRINET 59 Query: 64 -GVQDAVLEASRTK--PLFGVCVGEQMLFDWSEEGDTPGLGLLPGKVVRF 110 G++ A+ + + P+ GVC+G Q+L SEEG PGLG + G+ +RF Sbjct: 60 PGLRAALDHKALIERVPVIGVCLGMQLLTRSSEEGKLPGLGWIDGEAIRF 109 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.139 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 399,890 Number of extensions: 15484 Number of successful extensions: 57 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 57 Number of HSP's successfully gapped: 2 Length of query: 119 Length of database: 1,619,870 Length adjustment: 74 Effective length of query: 45 Effective length of database: 1,269,480 Effective search space: 57126600 Effective search space used: 57126600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 124 (52.4 bits)