BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0008.p (145 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313363|ref|YP_003777455.1| organic solvents resistance ABC... 274 2e-75 >gi|300313363|ref|YP_003777455.1| organic solvents resistance ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 158 Score = 274 bits (700), Expect = 2e-75, Method: Compositional matrix adjust. Identities = 136/145 (93%), Positives = 141/145 (97%) Query: 1 VLAGVAALAFLALKVGNMSSMSFGQKTYALKANFDNIGGLKSRAAVKSAGVVVGRVDSIR 60 VL GVAAL FLALK GNMSSMSFGQKTY +KA+FDNIGGLKSRAAVKSAGVVVGRVDSIR Sbjct: 14 VLLGVAALVFLALKAGNMSSMSFGQKTYTIKASFDNIGGLKSRAAVKSAGVVVGRVDSIR 73 Query: 61 FDDQTFRAVVTLNMNESYKFPKDSSAKILTSGLLGEQYIGIEPGGDTANLAAGDTIKMTQ 120 FDDQTFRAVV+LNM+ESYKFPKDSSAKILTSGLLGEQYIGIEPGGDTANLA+GDTIKMTQ Sbjct: 74 FDDQTFRAVVSLNMDESYKFPKDSSAKILTSGLLGEQYIGIEPGGDTANLASGDTIKMTQ 133 Query: 121 SAIVLENLIGQFLYSKAAEGKDDSK 145 SAIVLENLIGQFLYSKAAEGKDDSK Sbjct: 134 SAIVLENLIGQFLYSKAAEGKDDSK 158 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.132 0.353 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 354,032 Number of extensions: 13016 Number of successful extensions: 39 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 39 Number of HSP's successfully gapped: 1 Length of query: 145 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 68 Effective length of database: 1,255,275 Effective search space: 85358700 Effective search space used: 85358700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 126 (53.1 bits)