BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0023.0020.p (314 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309487|ref|YP_003773579.1| LysR family transcription regul... 577 e-166 gi|300313164|ref|YP_003777256.1| LysR family transcription regul... 307 8e-85 gi|300310701|ref|YP_003774793.1| LysR family transcription regul... 274 8e-75 gi|300310375|ref|YP_003774467.1| LysR family transcription regul... 149 3e-37 gi|300310484|ref|YP_003774576.1| LysR family transcription regul... 91 1e-19 >gi|300309487|ref|YP_003773579.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 314 Score = 577 bits (1487), Expect = e-166, Method: Compositional matrix adjust. Identities = 299/314 (95%), Positives = 305/314 (97%) Query: 1 MHTTLPSLASITSRLHLRQLRLLIALSDHGSLLKAAEQVALTQPGASKALQEIESTLGAQ 60 MH TLPSLASITSRLHLRQLRLLIALSDHGSLLKAAEQVALTQPGASKALQEIESTLG Q Sbjct: 1 MHNTLPSLASITSRLHLRQLRLLIALSDHGSLLKAAEQVALTQPGASKALQEIESTLGVQ 60 Query: 61 LFTRTNRGLEATELGHCVIRYARLIQTDLTHLREEMLSIMQGHGGRLAVGTIMGAVPLLT 120 LFTRTNRGLE TELGHCVIRYARLIQTDLTHLREEML+IMQGHGGRLAVGTIMGAVPLLT Sbjct: 61 LFTRTNRGLEPTELGHCVIRYARLIQTDLTHLREEMLAIMQGHGGRLAVGTIMGAVPLLT 120 Query: 121 DALTQLLEAHPTLSVEIVEDTSARLLRLLDQGRLDLAICRTSISQRPDLYDAFNIHEEEL 180 DALT+LLEAHP LSVEIVEDTSARLLRLLDQGRLDLAICRTSISQRPDLYDAFNIHEEEL Sbjct: 121 DALTRLLEAHPALSVEIVEDTSARLLRLLDQGRLDLAICRTSISQRPDLYDAFNIHEEEL 180 Query: 181 AVVASVQHPLAEASSLQLADLADSRWVVYSANMPMRLTLEREFHDADLRFPLHLMETTSA 240 AVVASVQHPLA+A SLQLADLADSRWVVYSANMPMRLTLEREFHDADLRFPLHLMETTSA Sbjct: 181 AVVASVQHPLADAPSLQLADLADSRWVVYSANMPMRLTLEREFHDADLRFPLHLMETTSA 240 Query: 241 FTTLSLLQKNPHFVALLSVDVAQFCTRFGMTRILPLKLHTRSEPYQLVTRRGTLLSPVAR 300 FTTLSLLQKNPHFVALLSVDVAQFCTRFGMTRILPL+LHTRSEPYQLVTRRGT+LSPVAR Sbjct: 241 FTTLSLLQKNPHFVALLSVDVAQFCTRFGMTRILPLRLHTRSEPYQLVTRRGTMLSPVAR 300 Query: 301 LFVDGFARAVAAPG 314 LF+D FAR VA G Sbjct: 301 LFLDDFARTVAPQG 314 >gi|300313164|ref|YP_003777256.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 324 Score = 307 bits (786), Expect = 8e-85, Method: Compositional matrix adjust. Identities = 152/301 (50%), Positives = 217/301 (72%), Gaps = 1/301 (0%) Query: 3 TTLPSLASITSRLHLRQLRLLIALSDHGSLLKAAEQVALTQPGASKALQEIESTLGAQLF 62 + LPS+ SI SRL RQ+ LL AL + GSL KAAE + +TQP A+KAL E+E LG LF Sbjct: 18 SELPSVGSIVSRLRFRQVALLTALDEQGSLHKAAEVMHMTQPAATKALHEMEDALGVTLF 77 Query: 63 TRTNRGLEATELGHCVIRYARLIQTDLTHLREEMLSIMQGHGGRLAVGTIMGAVPLLTDA 122 R+ RG+EATELG CVIRYARLIQ+D+ +LR+E+ +++ G GGRLA+GTIMGA P +T A Sbjct: 78 DRSPRGIEATELGRCVIRYARLIQSDVANLRDELQNMISGRGGRLAIGTIMGAAPFVTRA 137 Query: 123 LTQLLEAHPTLSVEIVEDTSARLLRLLDQGRLDLAICRTSISQRPDLYDAFNIHEEELAV 182 L++L E P +S+EI EDTSAR L LLDQGR+DL I R+S+S +P+LY + E + + Sbjct: 138 LSRLREIQPEVSIEISEDTSARQLLLLDQGRIDLMIGRSSVSSQPNLYHYEMLRGEPMCI 197 Query: 183 VASVQHPLAEASSLQLADLADSRWVVYSANMPMRLTLEREFHDADLRFPLHLMETTSAFT 242 V+ + HPLA A ++L +LA++ W++YS+NMPMR+ +E EF ++ P +++ET S F Sbjct: 198 VSGIDHPLATAQKVRLQELAEASWILYSSNMPMRIWIEHEFKLEGVKVPGNVIETASPFV 257 Query: 243 TLSLLQKNPHFVALLSVDVAQFCTRFGMTRILPLKLHTRSEPYQLVTRRGTLLSPVARLF 302 T++LL ++ + VA++ +D+A F M ILP+ L TR E Y +VTR+ T LS +A++F Sbjct: 258 TITLLAQS-NMVAVMPLDIAHFFAAKQMLGILPVNLKTRIEHYGIVTRKNTTLSSLAKMF 316 Query: 303 V 303 + Sbjct: 317 I 317 >gi|300310701|ref|YP_003774793.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 321 Score = 274 bits (700), Expect = 8e-75, Method: Compositional matrix adjust. Identities = 149/299 (49%), Positives = 192/299 (64%), Gaps = 1/299 (0%) Query: 7 SLASITSRLHLRQLRLLIALSDHGSLLKAAEQVALTQPGASKALQEIESTLGAQLFTRTN 66 SL S+ SRL ++QL+LLIAL +H SL KA+ +A+TQ ASK+L E+ES L AQLF RT Sbjct: 16 SLNSLLSRLRMKQLQLLIALDEHKSLHKASSALAMTQSAASKSLAELESMLDAQLFERTK 75 Query: 67 RGLEATELGHCVIRYARLIQTDLTHLREEMLSIMQGHGGRLAVGTIMGAVP-LLTDALTQ 125 GL A GHCVIRYARL+ TDL L EEM I G GGRLA+G +MGAVP L+ A+ Sbjct: 76 SGLIANPFGHCVIRYARLMATDLGSLCEEMAQIRSGQGGRLAIGAVMGAVPALIVPAVDA 135 Query: 126 LLEAHPTLSVEIVEDTSARLLRLLDQGRLDLAICRTSISQRPDLYDAFNIHEEELAVVAS 185 L HP LS+EIVEDTS +L LLD+G LDL + R S+S P Y ++ EE L+VV S Sbjct: 136 LQRRHPALSIEIVEDTSLHMLDLLDEGHLDLLVARASVSAHPGKYHYQSLSEEPLSVVVS 195 Query: 186 VQHPLAEASSLQLADLADSRWVVYSANMPMRLTLEREFHDADLRFPLHLMETTSAFTTLS 245 +HP LQLA LA RW+ Y ++MP+ LERE A L P + + T S F T++ Sbjct: 196 AEHPRPRGKELQLAALAGYRWITYPSHMPLHAVLEREMDLAGLSMPANTIATASTFVTVA 255 Query: 246 LLQKNPHFVALLSVDVAQFCTRFGMTRILPLKLHTRSEPYQLVTRRGTLLSPVARLFVD 304 LLQ+ V++L VA+ + GM RILP+KL + S+ +VTR+G LS + FV Sbjct: 256 LLQQGADMVSILPTAVAEMFVQRGMLRILPVKLRSMSQTIGIVTRKGGQLSGAGQAFVQ 314 >gi|300310375|ref|YP_003774467.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 313 Score = 149 bits (376), Expect = 3e-37, Method: Compositional matrix adjust. Identities = 103/302 (34%), Positives = 166/302 (54%), Gaps = 7/302 (2%) Query: 13 SRLHLRQLRLLIALSDHGSLLKAAEQVALTQPGASKALQEIESTLGAQLFTRTNRGLEAT 72 +RL RQL LLIAL D ++ +AA ++ +TQP ASK L+++E LG LF R RG+ T Sbjct: 12 ARLKTRQLLLLIALDDTRNIHRAASELNMTQPAASKQLKDLEDMLGVALFERLPRGMRPT 71 Query: 73 ELGHCVIRYARLIQTDLTHLREEMLSIMQGHGGRLAVGTIMG-AVPLLTDALTQLLEAHP 131 G +IR+AR+ T L+ E+++++ G G++ VG I+ A+ LL A+ ++ E P Sbjct: 72 IYGEAMIRHARMALTSLSKAHEDIVALKSGLSGQVDVGGILSPAMALLPPAIARVKEQAP 131 Query: 132 TLSVEIVEDTSARLLRLLDQGRLDLAICRTSISQRPD--LYDAFNIHEEELAVVASVQHP 189 L + + ++S LL L G LD I R + LY+ + +E + VA V HP Sbjct: 132 MLRIGVEVESSNILLSRLLHGELDFIIGRILDDEARGGLLYE--ELADEPICAVARVGHP 189 Query: 190 LAEASSLQLADLADSRWVVYSANMPMRLTLEREFHDADLRFPLHLMETTSAFTTLSLLQK 249 + L LADLA W++ +R ++ F + L P ++++TT+ SLLQ+ Sbjct: 190 FHARTDLSLADLASEAWIMAPKGSILRARCDQMFREQGLAPPSNVVDTTAILFITSLLQQ 249 Query: 250 NPHFVALLSVDVAQFCTRFGMTRILPLKLHTRSEPYQLVTRRGTLLSPVARLFVDGFARA 309 F+ + +VA++ T+ + ILP++L R + ++TR+ LLSP A L + R Sbjct: 250 T-DFIYAMPTEVARYYTQARLMDILPIELPCRMAGFGIITRQDNLLSPGANLVLQAI-RE 307 Query: 310 VA 311 VA Sbjct: 308 VA 309 >gi|300310484|ref|YP_003774576.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 322 Score = 90.9 bits (224), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 86/293 (29%), Positives = 136/293 (46%), Gaps = 9/293 (3%) Query: 15 LHLRQLRLLIALSDHGSLLKAAEQVALTQPGASKALQEIESTLGAQLFTRTNRGLEATEL 74 +L+QL + A+ GSL +AA + LTQP S+A++ +E ++G LF R +G+ T L Sbjct: 3 FNLQQLEMFNAIVSAGSLGRAAALLNLTQPALSRAIKRLEESVGGALFERHTKGMHLTVL 62 Query: 75 GHCVIRYARLIQTDLTHLREEMLSIMQGHGGRLAVGTIMGAVPL-LTDALTQLLEAHPTL 133 G ++ +A +Q + REE+ +I G + VG + L L A+ +L+ P L Sbjct: 63 GQALLPHAMSLQREAQQAREELDAIRGLAKGVIKVGAVGSIASLVLPLAVRGVLDKWPNL 122 Query: 134 SVEIVEDTSARLLRLLDQGRLDLAICRTSISQRPDLYDAFNIHEEELA-VVASVQHPLAE 192 VEI+E RL+ L +DLA+ TS Q + + +L+ VVA+ HPL + Sbjct: 123 RVEIIEGVWDRLVEGLLTHEIDLALS-TSGGQTEGVAAIADCCWTDLSYVVAAPDHPLRQ 181 Query: 193 ASSLQLADLADSRWVVYSANMPMRLTLEREFHDADLRFPLHLMETTSAFTTLSLLQKNPH 252 + L RW + L ++ F L P +E+ S T L L Sbjct: 182 QGRIALEQTLGQRWALTPKGTGPYLHVQETFARHGLPMPEIAVESRS-VTVLKSLVARCG 240 Query: 253 FVALLSVDVAQFCTRFGMTRILPLKLHTRSEPYQLVT---RRGTLLSPVARLF 302 F+ +S + F T I+ L++ P L R+G L +P ARL Sbjct: 241 FIGWMSGPM--FDTERQAGVIVALEVAGLDAPRTLTAFRRRQGILPAPAARLL 291 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.134 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 810,852 Number of extensions: 28139 Number of successful extensions: 181 Number of sequences better than 1.0e-08: 41 Number of HSP's gapped: 173 Number of HSP's successfully gapped: 41 Length of query: 314 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 229 Effective length of database: 1,217,395 Effective search space: 278783455 Effective search space used: 278783455 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 130 (54.7 bits)