BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0023.0016.p (364 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309490|ref|YP_003773582.1| branched-chain amino acid ABC t... 739 0.0 gi|300314071|ref|YP_003778163.1| amino acid ABC transporter peri... 536 e-153 gi|300312339|ref|YP_003776431.1| branched-chain amino acid ABC t... 489 e-139 gi|300312372|ref|YP_003776464.1| branched-chain amino acid ABC t... 332 2e-92 gi|300313931|ref|YP_003778023.1| branched-chain amino acid ABC t... 91 1e-19 >gi|300309490|ref|YP_003773582.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 739 bits (1909), Expect = 0.0, Method: Compositional matrix adjust. Identities = 356/364 (97%), Positives = 361/364 (99%) Query: 1 MSGTYADFDGQGGVEAIRMAIADAGGTINGKKVELVFADHQNKADIAANKAREWFDRDGV 60 MSGTYADFDGQGGVEAIRMAIADAGG INGKKVELVFADHQNKADIAANKAREWFDRDGV Sbjct: 41 MSGTYADFDGQGGVEAIRMAIADAGGAINGKKVELVFADHQNKADIAANKAREWFDRDGV 100 Query: 61 DMLVGGVNSAASLAMGKVAGEKKKVFISVGAGTTRQTNEECNAYTVQYAYDTTALARGTG 120 DMLVGGVNSAASLAMGKVAGEKKKVFISVGAGTTRQTNEECNAYT+QYAYDTTALARGTG Sbjct: 101 DMLVGGVNSAASLAMGKVAGEKKKVFISVGAGTTRQTNEECNAYTIQYAYDTTALARGTG 160 Query: 121 AAITRQGGKSWYFLTADYAFGTSLEKDTSEVVKSNGGNVVGATRVPLATSDFSSFLLQAQ 180 AAITRQGGKSWYFLTADYAFGTSLEKDTSEVVKSNGGNVVGATRVPLATSDFSSFLLQAQ Sbjct: 161 AAITRQGGKSWYFLTADYAFGTSLEKDTSEVVKSNGGNVVGATRVPLATSDFSSFLLQAQ 220 Query: 181 SSKAQILGLAVAGGDVINAIKAANEFGVNKTMKLAGLLIFINDTHSLGLQMTQGMYLTDG 240 SSKAQILGLAVAGGDVINAIKAANEFGVNKTMKLAGLLIFINDTHSLGL +TQGMYLTDG Sbjct: 221 SSKAQILGLAVAGGDVINAIKAANEFGVNKTMKLAGLLIFINDTHSLGLSLTQGMYLTDG 280 Query: 241 WYWDLNDESRAWANRYFMKMKKMPSMFQAGDASAVGQYLKAVKAVGSTDADQVMDYLRKN 300 WYWDLND++RAWANRYFMKMKKMPSMFQAGDASAVGQYLKAVKAVGSTDAD+VMDYLRKN Sbjct: 281 WYWDLNDQTRAWANRYFMKMKKMPSMFQAGDASAVGQYLKAVKAVGSTDADKVMDYLRKN 340 Query: 301 RIKDFFTSNGVVRPDGRMVHDMYLMEVKKPSESKRPWDYYKLVQTIPGEQAYMTKAESKC 360 RI DFFTSNGVVRPDGRMVHDMYLMEVKKPSESKRPWDYYKLVQTIPGEQAYMTKAESKC Sbjct: 341 RINDFFTSNGVVRPDGRMVHDMYLMEVKKPSESKRPWDYYKLVQTIPGEQAYMTKAESKC 400 Query: 361 ALWK 364 ALWK Sbjct: 401 ALWK 404 >gi|300314071|ref|YP_003778163.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 402 Score = 536 bits (1380), Expect = e-153, Method: Compositional matrix adjust. Identities = 251/364 (68%), Positives = 297/364 (81%) Query: 1 MSGTYADFDGQGGVEAIRMAIADAGGTINGKKVELVFADHQNKADIAANKAREWFDRDGV 60 +SG Y+D DGQGG EAI+MAIAD GG INGKK+EL+ ADHQNKAD+AA+KAREWFD+ GV Sbjct: 39 ISGVYSDIDGQGGAEAIKMAIADMGGAINGKKIELLVADHQNKADVAASKAREWFDQQGV 98 Query: 61 DMLVGGVNSAASLAMGKVAGEKKKVFISVGAGTTRQTNEECNAYTVQYAYDTTALARGTG 120 DML+GG NS +LA KVA EKKKV++ +GAG+ R TNEEC+ YTV YAYDT ALA+GTG Sbjct: 99 DMLIGGTNSGTALASAKVAAEKKKVYMQIGAGSARLTNEECSPYTVHYAYDTVALAKGTG 158 Query: 121 AAITRQGGKSWYFLTADYAFGTSLEKDTSEVVKSNGGNVVGATRVPLATSDFSSFLLQAQ 180 +A+ +QGGKSW+FLTADYAFG SLE DT+ VVK++GG VVGA + PL SDFSSFLLQAQ Sbjct: 159 SAVVKQGGKSWFFLTADYAFGHSLESDTANVVKASGGTVVGAVKHPLGASDFSSFLLQAQ 218 Query: 181 SSKAQILGLAVAGGDVINAIKAANEFGVNKTMKLAGLLIFINDTHSLGLQMTQGMYLTDG 240 SSKAQI+GLA AGGD IN++KAANEFG+ KT KLAGLL+FIND HSL L+ T+G+YLTD Sbjct: 219 SSKAQIIGLANAGGDFINSVKAANEFGITKTAKLAGLLVFINDVHSLTLKNTEGLYLTDS 278 Query: 241 WYWDLNDESRAWANRYFMKMKKMPSMFQAGDASAVGQYLKAVKAVGSTDADQVMDYLRKN 300 WYWD N+E+RA+A RYF KKMPS QA D SA QYLK VK +G+ D+D+VM LRK Sbjct: 279 WYWDANEETRAFAKRYFAVRKKMPSSLQAADYSAALQYLKVVKEIGTDDSDKVMAALRKA 338 Query: 301 RIKDFFTSNGVVRPDGRMVHDMYLMEVKKPSESKRPWDYYKLVQTIPGEQAYMTKAESKC 360 + D + NG +R DGR++HDMYL +VK SESK PWDYYKLVQTIP EQA+ TKAESKC Sbjct: 339 KFNDMYQKNGYLRADGRVMHDMYLYQVKAQSESKYPWDYYKLVQTIPAEQAWTTKAESKC 398 Query: 361 ALWK 364 A WK Sbjct: 399 AAWK 402 >gi|300312339|ref|YP_003776431.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 489 bits (1259), Expect = e-139, Method: Compositional matrix adjust. Identities = 230/364 (63%), Positives = 286/364 (78%), Gaps = 1/364 (0%) Query: 1 MSGTYADFDGQGGVEAIRMAIADAGGTINGKKVELVFADHQNKADIAANKAREWFDRDGV 60 MSG YAD DG GG+EAIRMA+AD GGT+ GK +E++ ADHQNKADIAA +AREW D G+ Sbjct: 41 MSGPYADTDGLGGLEAIRMAVADFGGTVLGKPIEVISADHQNKADIAATRAREWADERGL 100 Query: 61 DMLVGGVNSAASLAMGKVAGEKKKVFISVGAGTTRQTNEECNAYTVQYAYDTTALARGTG 120 DML+GGVNSA +L+M KV EKK+V+I++GAGT R TNEEC YTV Y YDT ALA+GT Sbjct: 101 DMLIGGVNSATALSMNKVMAEKKRVYINIGAGTARLTNEECTPYTVHYEYDTVALAKGTA 160 Query: 121 AAITRQGGKSWYFLTADYAFGTSLEKDTSEVVKSNGGNVVGATRVPLATSDFSSFLLQAQ 180 +A+ +QGGKSW+FL ADYAFG SL DTS VVK++GG VVG+ + P +SD SSFLLQAQ Sbjct: 161 SAVIKQGGKSWFFLVADYAFGHSLANDTSAVVKASGGTVVGSIKHPPQSSDLSSFLLQAQ 220 Query: 181 SSKAQILGLAVAGGDVINAIKAANEFGVNKTMKLAGLLIFINDTHSLGLQMTQGMYLTDG 240 +SKAQILGLA AG D +N+IKAA EFGV KTMKL GLL+ IND H+LGL +G+ +TD Sbjct: 221 ASKAQILGLANAGEDTVNSIKAAKEFGVTKTMKLVGLLMVINDIHALGLANAEGLMMTDS 280 Query: 241 WYWDLNDESRAWANRYFMKMKKMPSMFQAGDASAVGQYLKAVKAVGSTDADQVMDYLRKN 300 WYWD ++ SR +A R+F KMKKMPS QA SA YLKAV+A+G+ D+ +VM L+K Sbjct: 281 WYWDKDEPSRKFAERFFQKMKKMPSTHQAAAYSATTTYLKAVQAIGTDDSTKVMAQLKKT 340 Query: 301 RIKDFFTSNGVVRPDGRMVHDMYLMEVKKPSESKRPWDYYKLVQTIPGEQAYMTKAESKC 360 RI DF+ + G +R DGR +HDMYL +VK P+ESK+PWDY KL++TIPGEQA+ + A+SKC Sbjct: 341 RIDDFY-NKGSIRADGRNIHDMYLYQVKSPAESKKPWDYLKLLETIPGEQAFTSVADSKC 399 Query: 361 ALWK 364 +L K Sbjct: 400 SLLK 403 >gi|300312372|ref|YP_003776464.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 410 Score = 332 bits (852), Expect = 2e-92, Method: Compositional matrix adjust. Identities = 174/367 (47%), Positives = 239/367 (65%), Gaps = 4/367 (1%) Query: 1 MSGTYADFDGQGGVEAIRMAIADA-GGTINGKKVELVFADHQNKADIAANKAREWFDRDG 59 MSG Y+ GQG V A +MA+ D G K+E++ AD+QNK D+ +AREW DRD Sbjct: 44 MSGPYSAMGGQGSVVAAKMAVEDCLKNECKGMKIEILSADNQNKPDVGVTRAREWLDRDK 103 Query: 60 VDMLVGGVNSAASLAMGKVAGEKKKVFISVGAGTTRQTNEECNAYTVQYAYDTTALARGT 119 V+ + NS+ +LA+ K+ +K + + G T T EC + +DT + A G Sbjct: 104 VEAIADLTNSSVALAVQKLIKDKGGIAMYSGPATGALTTAECAPNGFHWMFDTYSQAAGA 163 Query: 120 GAAITRQGGKSWYFLTADYAFGTSLEKDTSEVVKSNGGNVVGATRVPLATSDFSSFLLQA 179 AA+T+ G KSWYF+T DYAFG SLEKD S+VVK+NGG VVG+ R PL SDFSSFLLQA Sbjct: 164 AAALTKLGNKSWYFVTVDYAFGHSLEKDASDVVKANGGTVVGSVRHPLNASDFSSFLLQA 223 Query: 180 QSSKAQILGLAVAGGDVINAIKAANEFGV-NKTMKLAGLLIFINDTHSLGLQMTQGMYLT 238 Q+SKAQ++GLA G DV+NAIK A F V K +L LL+F++D H+LGL+ QG+ T Sbjct: 224 QASKAQVIGLADGGTDVVNAIKQARSFAVGGKDQRLVALLVFLSDVHALGLEAAQGLVYT 283 Query: 239 DGWYWDLNDESRAWANRYFMKMKKM-PSMFQAGDASAVGQYLKAVKAVGSTDADQVMDYL 297 DG+YWD ++++RA++ R+ + K + P+M QAG S+V Q+LKA A ++D V + Sbjct: 284 DGFYWDYDEQTRAFSKRFESQFKNIKPTMVQAGVYSSVYQFLKARAAAKTSDWKVVAQKM 343 Query: 298 RKNRIKDFFTSNGVVRPDGRMVHDMYLMEVKKPSESKRPWDYYKLVQTIPGEQAYMTKAE 357 R+ I D N +RPDGR++HDMYL +VK P+ESK PWDY KL+ IP +QA+ + Sbjct: 344 REIPIADAVMRNASIRPDGRVIHDMYLYQVKTPAESKGPWDYLKLLSVIPAQQAF-RPMD 402 Query: 358 SKCALWK 364 + C L K Sbjct: 403 AACPLVK 409 >gi|300313931|ref|YP_003778023.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 397 Score = 90.9 bits (224), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 97/374 (25%), Positives = 164/374 (43%), Gaps = 27/374 (7%) Query: 1 MSGTYADFDGQGGVEAIRMAIADAGGTINGKKVELVFADHQNKADIAANKAREWFDRDGV 60 MSG +A +G+ +++A+ G + GK V D + K A K +E ++ G Sbjct: 39 MSGPFAA-NGKFADLGMKLALQKYGKVL-GKSTSYVNLDTEGKPATAVRKMQESIEK-GT 95 Query: 61 DMLVGGVNSAASLAMGKVAGEKKKVFISVGAGTTRQTNEECNAYTVQYAYDTTALARGTG 120 GG+ S +LAMGK A + VFI+ AG T +CN T +++ T T Sbjct: 96 RFFAGGILSGEALAMGKEADKAGAVFITT-AGADEITGSDCNRATFRWSVPTFGAIEQTV 154 Query: 121 AAITRQ--GGKSWYFLTADYAFGTSLEKDTSEVVKSNGGNVVGATRVPLATSDFSSFLLQ 178 + + K WY +T Y FG L + K G VG + L +FS +L Sbjct: 155 RPLLEKMPNAKRWYTITPQYVFGEGLLSAAKNIFKEKGIEHVGNSYHSLTEKEFSGYLTN 214 Query: 179 AQSSKAQILGLAVAGGDVINAIKAANEFGV--NKTMKLA---GLLIFINDTHSLGLQMTQ 233 A ++K +L L G + ++ A FG+ N T+ +A GL F +LG + Sbjct: 215 AIAAKPDVLLLLNFGSQSSDTLRQAVSFGMKNNCTILMAWASGLEQF----ETLGADICD 270 Query: 234 GMYLTDGWYWDLNDESRAWANRYFMK-----MKKMPSMFQAGDASAVGQYLKAVKAVGST 288 G+Y YW D A N+ F+K +K P+ AG L A+ ST Sbjct: 271 GVYF-GAQYWHAID---APFNKEFVKLVNDNLKIDPNYSLAGSYICTKIILDAIIKANST 326 Query: 289 DADQVMDYLRKNRIKDFFTSNGVVRPDGRMVHDMYLMEVKKPSESKRPWDYYKLVQTIPG 348 D V+ + + + S + + D +++ + YL++ K ++ DY +V + Sbjct: 327 DPKAVIAAMEGMKYEGLTGSEEIRKGDHQVIKNYYLLKGKAKAKMANKNDYADIVSS--- 383 Query: 349 EQAYMTKAESKCAL 362 ++++ +++C L Sbjct: 384 GKSFLPLEKTQCKL 397 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.131 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,115,014 Number of extensions: 45918 Number of successful extensions: 179 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 174 Number of HSP's successfully gapped: 10 Length of query: 364 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 278 Effective length of database: 1,212,660 Effective search space: 337119480 Effective search space used: 337119480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)