BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0316.p (678 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314078|ref|YP_003778170.1| uncharacterized protein involve... 1077 0.0 gi|300311615|ref|YP_003775707.1| outer membrane biogenesis prote... 363 e-101 >gi|300314078|ref|YP_003778170.1| uncharacterized protein involved in outer membrane biogenesis protein [Herbaspirillum seropedicae SmR1] Length = 682 Score = 1077 bits (2785), Expect = 0.0, Method: Compositional matrix adjust. Identities = 544/682 (79%), Positives = 614/682 (90%), Gaps = 4/682 (0%) Query: 1 MPRLTRILLWLLASLILVIAAAVIFILTFDWNRAKPWINTRVSAATGREFAIDGNLSLHW 60 MPRLT+I+LWL ASLIL+I AAVIFIL+FDWNRAKPWIN RVSA+ GREFAIDG+LSLHW Sbjct: 1 MPRLTKIILWLCASLILLITAAVIFILSFDWNRAKPWINERVSASIGREFAIDGDLSLHW 60 Query: 61 QRPE--PTTPPAGFWARWVPWPRLQAENLRLGNPAAFASSGQMATVRQVTFTLNPLPLLV 118 QR + P P GFW RW+PWPRLQAE LR+GNP AF+ +GQMAT+ QVTFTLNPLPLLV Sbjct: 61 QRAQTLPGAAPPGFWERWLPWPRLQAEQLRIGNPPAFSQAGQMATIGQVTFTLNPLPLLV 120 Query: 119 HKISLPSLEVQDPSVSLLRNREAVNNWTIKTDSSTPSAWQLQLGRISLRHGKLALNDAVQ 178 H I+LPSL+++DPSVSLLRN +AVNNWT K+ S+ PSAW L+LGRI+LR GKL+L+DAV Sbjct: 121 HDIALPSLDIRDPSVSLLRNAQAVNNWTFKSSSAGPSAWNLRLGRITLRQGKLSLDDAVD 180 Query: 179 KINIKADIDTLDPDKEKVYGLGWKLSGSFNGAPVAGEGKAGGVLSLQDQQQPYPLQAQVK 238 KI +KA+IDTLDP EK+YGLGWKLSGSFNGAPV+G+GKAGGVLSLQ+ QQPYPLQAQV+ Sbjct: 181 KIKLKAEIDTLDPAVEKIYGLGWKLSGSFNGAPVSGQGKAGGVLSLQNVQQPYPLQAQVR 240 Query: 239 VGKTTIGVRGTLTKPAELAALDLRLSIAGASMGNLYPLTGVLLPETPPFATEGRLIGKIS 298 VGKT IGV GTLT+PA LAALDLRLS++GASMGNLYPLTGVLLPETPPFATEGRLIGKI Sbjct: 241 VGKTAIGVHGTLTRPAALAALDLRLSVSGASMGNLYPLTGVLLPETPPFATEGRLIGKID 300 Query: 299 PDRSEWHYQDFSGKVGDSDLNGSLDYLTGG--ARPQLTGALTSNVLRFDDLAPLIGADSN 356 S+W Y+DF GKVG SD+NGSL Y TG AR QLTG+LTS VLRFDDLAPLIGADSN Sbjct: 301 AGHSQWDYEDFRGKVGQSDINGSLRYTTGAGAARSQLTGSLTSEVLRFDDLAPLIGADSN 360 Query: 357 AEKAQRGAEERQPGDKVLPVKTFSTDAWGALDADVRFTGKSITRQQGLPISDLQAHLILK 416 AEKA+RGA+ERQPGDKVLPVKTFSTDAWGALDADV+ TGK I +Q+ LPISDLQAHLILK Sbjct: 361 AEKAERGAQERQPGDKVLPVKTFSTDAWGALDADVKLTGKRIIKQKNLPISDLQAHLILK 420 Query: 417 DKVLTLDPLNFGVAGGNLTSTIRLDGQAARMKSEMKLSARKLKIQKLYPQLDASRTSFGE 476 DKVLTL PL+FGVAGGNL STIRLDGQAA MKSE+ LSAR+LKIQKLYPQLDASRTSFGE Sbjct: 421 DKVLTLAPLDFGVAGGNLRSTIRLDGQAAPMKSELILSARRLKIQKLYPQLDASRTSFGE 480 Query: 477 INGDAKLSATGNSVAAMLGSANGEVKTLVNQGAISKFLLEAAGLNVGNVVISKLFGDKEV 536 INGDA+LSATGNSVAAMLGS+NGEVKTLV QGAISKFLLEAAGLN+GNVVISKLFGDKEV Sbjct: 481 INGDARLSATGNSVAAMLGSSNGEVKTLVTQGAISKFLLEAAGLNLGNVVISKLFGDKEV 540 Query: 537 RLNCLAGDLPVRDGVMNVKTLLLDTEDALIVVDGSIDMKHEQLDLNVHPRSKGLRIISLR 596 +LNCLAGDLPVR+G+M +TL+LDTEDALI VDG+IDM+ E+LDL+VHP+SKGLRIISLR Sbjct: 541 KLNCLAGDLPVREGIMTARTLMLDTEDALISVDGTIDMRREKLDLHVHPKSKGLRIISLR 600 Query: 597 SPLYVKGSFKNPDVGVNKGVLALRAGGAVALGLLAPVTAILPLINIDGDQKAECGQLLEE 656 SPLYV G+FK PDVGV+KG+LALRAG AVALGLLAPVTAILPLIN+DGD++A+C LLEE Sbjct: 601 SPLYVAGTFKQPDVGVDKGMLALRAGSAVALGLLAPVTAILPLINVDGDEQADCAHLLEE 660 Query: 657 VKKAPQAPPPGKSLPLKSTPKK 678 +KAP+AP GK+LP + +PKK Sbjct: 661 ARKAPRAPAAGKTLPAERSPKK 682 >gi|300311615|ref|YP_003775707.1| outer membrane biogenesis protein [Herbaspirillum seropedicae SmR1] Length = 654 Score = 363 bits (931), Expect = e-101, Method: Compositional matrix adjust. Identities = 217/595 (36%), Positives = 325/595 (54%), Gaps = 30/595 (5%) Query: 30 DWNRAKPWINTRVSAATGREFAIDGNLSLHWQRPEPTTPPAGFWARWVPWPRLQAENLRL 89 DWN AKPW+ ++SAA GR+ +DGNL L W + AG AR P RL+AE L + Sbjct: 41 DWNSAKPWLGRQLSAALGRDIRVDGNLDLSWSLAQ-ADDEAGALARLAPQLRLRAERLFI 99 Query: 90 GNPAAFASSGQMATVRQVTFTLNPLPLLVHKISLPSLEVQDPSVSLLRNREAVNNWTIKT 149 GNP SS + + +PLPLL + L V+ + + R + NW Sbjct: 100 GNPDWAHSSDHLVRAAALDVRFSPLPLLHWHWHVTDLRVEGLDLVMERADDQRKNWRF-- 157 Query: 150 DSSTP-SAWQLQLGRISLRHGKLALNDAVQKINIKADIDTLDP-----DKEKVYGLGWKL 203 SS P S W + RI H + + ++++ D L+ L + Sbjct: 158 -SSQPRSRWSFDIHRIVFGHALIRYVELPLDLDLRFDARPLNATATNQKDSAALVLQAAV 216 Query: 204 SGSFNGAPVAGEGKAGGVLSLQDQQQPYPLQAQVKVGKTTIGVRGTLTKPAELAALDLRL 263 +G + A V G+ K G + L ++ +PLQA+ +G + G L P +L+ DLR+ Sbjct: 217 TGRYGQAEVNGQIKGGALPDLLNEGSVFPLQAEGDIGGVQTQLSGNLINPHQLSRADLRI 276 Query: 264 SIAGASMGNLYPLTGVLLPETPPFATEGRL-IGKISPDRS--EWHYQDFSGKVGDSDLNG 320 +AG S+ LYP TGV LP T F TEG L I ++ P +W Y FSGKVG+SD G Sbjct: 277 GLAGDSLDRLYPATGVPLPATRAFHTEGALTITRLDPQTKAWDWRYSGFSGKVGESDFAG 336 Query: 321 SLDYLTGGARPQLTGALTSNVLRFDDLAPLIGADSNAEKAQRGAEERQPGDKVLPVKTFS 380 Y+ G + L + +LR D P G D++ K P K Sbjct: 337 DAQYVRGPPKNHLKVKAEAGLLRLSDYLP--GDDADQGK---------------PSKPAQ 379 Query: 381 TDAWGALDADVRFTGKSITRQQGLPISDLQAHLILKDKVLTLDPLNFGVAGGNLTSTIRL 440 + W LDAD+ K + + GL A L LKD+VLT DPL F +AGG +RL Sbjct: 380 PERWSKLDADIDLQAKRVQWRAGLEWQQAAATLTLKDQVLTADPLRFNMAGGQGQGQVRL 439 Query: 441 DGQAARMKSEMKLSARKLKIQKLYPQLDASRTSFGEINGDAKLSATGNSVAAMLGSANGE 500 DG+ ++S +++ + ++++L+P+L + + SF + NG+A LSATGNS AA+L SA+GE Sbjct: 440 DGRQPDIRSRLQIRLQDAQVRELFPRLASLKASFSKFNGEATLSATGNSAAAVLASADGE 499 Query: 501 VKTLVNQGAISKFLLEAAGLNVGNVVISKLFGDKEVRLNCLAGDLPVRDGVMNVKTLLLD 560 +K + G IS+F+LEAAGLN+ N V +KL+ D++V+L C A D+ ++ G ++ +L+ Sbjct: 500 IKADLGPGTISQFILEAAGLNLANAVFAKLYRDQQVKLLCGAADVAIQHGQATIRHGILN 559 Query: 561 TEDALIVVDGSIDMKHEQLDLNVHPRSKGLRIISLRSPLYVKGSFKNPDVGVNKG 615 TEDA I V G +D+ + L L++HPR+K +RI+SLR+PL+V+GSF +P++G + G Sbjct: 560 TEDAAIDVGGQVDLGRQTLALDIHPRTKQVRILSLRTPLHVRGSFSHPEIGADSG 614 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,096,806 Number of extensions: 90375 Number of successful extensions: 275 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 269 Number of HSP's successfully gapped: 2 Length of query: 678 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 586 Effective length of database: 1,184,250 Effective search space: 693970500 Effective search space used: 693970500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 134 (56.2 bits)