BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0311.p (539 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314074|ref|YP_003778166.1| choline dehydrogenase [Herbaspi... 1080 0.0 gi|300314045|ref|YP_003778137.1| choline dehydrogenase [Herbaspi... 328 7e-91 >gi|300314074|ref|YP_003778166.1| choline dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 539 Score = 1080 bits (2794), Expect = 0.0, Method: Compositional matrix adjust. Identities = 516/539 (95%), Positives = 525/539 (97%) Query: 1 MQSAGQYDYIIIGAGTAGCVMANRLSRQTGKKVLLLEAGAKDDYIWIHIPVGYLYCINNP 60 MQSAGQYDYIIIGAGTAGCVMANRLSR+TGKKVLL+EAGAKDDYIWIHIPVGYLYCINNP Sbjct: 1 MQSAGQYDYIIIGAGTAGCVMANRLSRKTGKKVLLIEAGAKDDYIWIHIPVGYLYCINNP 60 Query: 61 RTDWMFRTEADPGLNGRSLIYPRGKVLGGCSSINGMIYMRGQARDYDHWAEVTGDDSWRW 120 RTDWMFRTEAD GLNGRSLIYPRGKVLGGCSSINGMIYMRGQARDYDHWA+VTGDDSWRW Sbjct: 61 RTDWMFRTEADAGLNGRSLIYPRGKVLGGCSSINGMIYMRGQARDYDHWADVTGDDSWRW 120 Query: 121 QNVLPLFKKSEDYHLGASQFHGAGGEWRVEKQRLRWDILDAFRDAAAENGIPKVEDFNCG 180 QNVLPLFKKSEDYHLGA QFHGAGGEWRVEKQRLRWDILDAFRDAAAENGIPKVEDFNCG Sbjct: 121 QNVLPLFKKSEDYHLGAGQFHGAGGEWRVEKQRLRWDILDAFRDAAAENGIPKVEDFNCG 180 Query: 181 DNEGSGYFDVNQKRGVRWNASKAFLRPAMKDGNLTIMTGSHVSRLRMEQGEQGPVCTGVE 240 DNEG GYFDVNQKRGVRWNASKAFLRPAMKDGNLTIMTGSHVSRLRMEQGEQGPVCTGVE Sbjct: 181 DNEGCGYFDVNQKRGVRWNASKAFLRPAMKDGNLTIMTGSHVSRLRMEQGEQGPVCTGVE 240 Query: 241 FTGGGSAWFAEAKETILCAGAIGSPHILQMSGIADPVLLQQHQIPVVHALPGVGENLQDH 300 FTGGGSAWFAEAKETILCAGAIGSPHILQMSGIADP LLQQHQIPVVHALPGVGENLQDH Sbjct: 241 FTGGGSAWFAEAKETILCAGAIGSPHILQMSGIADPALLQQHQIPVVHALPGVGENLQDH 300 Query: 301 LQMRMVFKVDGARTLNAMASTLVGKMKIGLEYLLLQSGPMSMAPSQLGAFARSDAQQASA 360 LQMRMVFKV+GA+TLNAMASTLVGKM+IGL+YL QSGPMSMAPSQLGAFA+SDAQQASA Sbjct: 301 LQMRMVFKVNGAKTLNAMASTLVGKMQIGLQYLFTQSGPMSMAPSQLGAFAKSDAQQASA 360 Query: 361 NLQYHVQPLSLEKFGDPLHAFPAFTASVCNLRPTSRGHVRLGSTDHALAPRITTNYLSTE 420 NLQYHVQPLSLEKFGDPLHAFPAFTASVCNLRPTSRGHVRL S DHALAPRITTNYLSTE Sbjct: 361 NLQYHVQPLSLEKFGDPLHAFPAFTASVCNLRPTSRGHVRLASGDHALAPRITTNYLSTE 420 Query: 421 EDLKVAANALRLTRRIAASPALSRYRPEEYKPGTQYESEADLYRAAGEVGTTIFHPVGTC 480 EDLKVAANALRLTRRIAASPALSRYRPEEYKPG YE+E DLYRAAGEVGTTIFHPVGTC Sbjct: 421 EDLKVAANALRLTRRIAASPALSRYRPEEYKPGAHYETEEDLYRAAGEVGTTIFHPVGTC 480 Query: 481 RMGRDDDAMAVVDAQCRVRGVSGLSVADASVMPSITSGNTNSPTVMIAEKVASSLLSRA 539 RMGR DDAMAVVDAQ RVRGV GLSVADASVMPSITSGNTNSPTVMIAEKVASSLLSRA Sbjct: 481 RMGRSDDAMAVVDAQGRVRGVGGLSVADASVMPSITSGNTNSPTVMIAEKVASSLLSRA 539 >gi|300314045|ref|YP_003778137.1| choline dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 543 Score = 328 bits (841), Expect = 7e-91, Method: Compositional matrix adjust. Identities = 204/538 (37%), Positives = 279/538 (51%), Gaps = 16/538 (2%) Query: 7 YDYIIIGAGTAGCVMANRLSRQTGKKVLLLEAGAKDDYIWIHIPVGYLYCINN--PRTDW 64 +DY+I+G G+AG +A RLS V LLEAG + D + + P + + +W Sbjct: 3 FDYVIVGGGSAGATLAARLSEDPRISVCLLEAGGQGDSLLVRTPAAVVAMLPGYGKLNNW 62 Query: 65 MFRTEADPGLNGRSLIYPRGKVLGGCSSINGMIYMRGQARDYDHWAEVTGDDSWRWQNVL 124 +T PGLNGR PRG+ LGG S+IN M+Y+RGQ +DYD WA+ G W W++VL Sbjct: 63 ALQTTPQPGLNGRRGYQPRGRALGGSSAINAMLYVRGQRQDYDGWAQ-AGCPGWDWESVL 121 Query: 125 PLFKKSEDYHLGASQFHGAGGEWRVEKQRLRWDILDAFRDAAAENGIPKVEDFNCGDNEG 184 P FK++E+ GA +HGA G +V +Q I AF +A G +DFN GDNEG Sbjct: 122 PYFKRAENNVRGADAWHGASGPLQVSEQNRPRPITRAFIEAGQARGHRLCQDFNTGDNEG 181 Query: 185 SGYFDVNQ-----KRGVRWNASKAFLRPAM-KDGNLTIMTGSHVSRLRMEQGEQGPVCTG 238 G + V Q RG R +A+ A+L P M + NL+++ RL E G G Sbjct: 182 VGLYQVTQFHTPAHRGERCSAAAAYLHPVMGQRPNLSVLRQVRAQRLLFE----GKRAIG 237 Query: 239 VEF-TGGGSAWFAEAKETILCAGAIGSPHILQMSGIADPVLLQQHQIPVVHALPGVGENL 297 V + G A A+E I+ AGA GSP +LQ+SG+ + H I + H LPGVG+NL Sbjct: 238 VAYRQQQGDAQVRAAREVIIAAGAFGSPQLLQLSGVGRSDDILPHGIALHHPLPGVGQNL 297 Query: 298 QDHLQMRMVFKVDGARTLNAMASTLVGKMKIGLEYLLLQSGPMSMAPSQLGAFARSDAQQ 357 QDHL + + + L + G ++ ++ AF ++ + Sbjct: 298 QDHLDFTQGWTTRDTDNFGLGVVGGLRLLGQLLPWKRHGEGLIATPFAEGAAFLKTRPEL 357 Query: 358 ASANLQYHVQPLSLEKFGDPLHAFPAFTASVCNLRPTSRGHVRLGSTDHALAPRITTNYL 417 ++Q H ++ LHA F+ +C LRP SRG V L S D P I YL Sbjct: 358 DRPDIQLHFCIAIVDDHARKLHAGYGFSLHMCMLRPHSRGRVGLQSADPMADPLIDPGYL 417 Query: 418 STEEDLKVAANALRLTRRIAASPALSRYRPEEYKPGTQYESEADLYRAAGEVGTTIFHPV 477 S DL + R+ R+I + L Y E G +A TI+HP Sbjct: 418 SDPRDLATMIDGARMARQIVMTEPLRHYCRRELFGGRDDMDDAQWESMIRHRADTIYHPA 477 Query: 478 GTCRMGRDDDAMAVVDAQCRVRGVSGLSVADASVMPSITSGNTNSPTVMIAEKVASSL 535 GTCRMG +DAMAVVDAQ RV G+ GL V DASVMP++ SGNTN+PT+MIAEK A + Sbjct: 478 GTCRMG--EDAMAVVDAQLRVHGLQGLRVVDASVMPTLVSGNTNAPTIMIAEKAADMI 533 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,796,163 Number of extensions: 79838 Number of successful extensions: 239 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 233 Number of HSP's successfully gapped: 2 Length of query: 539 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 449 Effective length of database: 1,193,720 Effective search space: 535980280 Effective search space used: 535980280 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)