BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0306.p (294 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314070|ref|YP_003778162.1| branched-chain amino acid ABC t... 561 e-161 gi|300313932|ref|YP_003778024.1| branched-chain amino acid ABC t... 181 7e-47 gi|300312954|ref|YP_003777046.1| amino acid ABC transporter perm... 153 2e-38 gi|300312237|ref|YP_003776329.1| amino acid ABC transporter perm... 138 6e-34 gi|300313286|ref|YP_003777378.1| branched-chain amino acid ABC t... 130 2e-31 >gi|300314070|ref|YP_003778162.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 294 Score = 561 bits (1447), Expect = e-161, Method: Compositional matrix adjust. Identities = 289/294 (98%), Positives = 293/294 (99%) Query: 1 MELFGIPLQALLSQLLLGLVNGSFYAMLSLGLAVIFGLLNVINFAHGTLYMLGAFLAWMG 60 MELFGIPLQALLSQLLLGLVNGSFYAMLSLGLAVIFGLLNVINFAHGTLYMLGAFLAWMG Sbjct: 1 MELFGIPLQALLSQLLLGLVNGSFYAMLSLGLAVIFGLLNVINFAHGTLYMLGAFLAWMG 60 Query: 61 LTYLGLNYWVMLIVAPILVGLFGIVIEKTMLRWLYKLDHLYGLLLTFGITLMIEGIFRSI 120 L+YLGLNYWVMLIVAPILVG FGIVIEKTMLRWLYKLDHLYGLLLTFGITLMIEGIFRSI Sbjct: 61 LSYLGLNYWVMLIVAPILVGAFGIVIEKTMLRWLYKLDHLYGLLLTFGITLMIEGIFRSI 120 Query: 121 YGVSGQPYSVPDLLQGATNLGFMVLPNYRAWVVVASLVICFATWFVIEKTRLGAYLRAGT 180 YGVSGQPYSVPDLLQGATNLGFMVLPNYRAWVVVASL++CFATWFVIEKTRLGAYLRAGT Sbjct: 121 YGVSGQPYSVPDLLQGATNLGFMVLPNYRAWVVVASLIVCFATWFVIEKTRLGAYLRAGT 180 Query: 181 ENPKLVEAFGINVPLMVTLTYGFGVALAAFAGVLAAPVIQVSPLMGSNLIITVFAVVVIG 240 ENPKLVEAFGINVPLMVTLTYGFGVALAAFAGVLAAPVIQVSPLMGSNLIITVFAVVVIG Sbjct: 181 ENPKLVEAFGINVPLMVTLTYGFGVALAAFAGVLAAPVIQVSPLMGSNLIITVFAVVVIG 240 Query: 241 GMGSILGSIITGLVLGLIEGLTRVFYPELSATVVFIVMAIVLMVRPAGLFGKEK 294 GMGSILGSIITGLVLGLIEGLTRVFYPELSATVVFI+MAIVLMVRPAGLFGKEK Sbjct: 241 GMGSILGSIITGLVLGLIEGLTRVFYPELSATVVFIIMAIVLMVRPAGLFGKEK 294 >gi|300313932|ref|YP_003778024.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 288 Score = 181 bits (459), Expect = 7e-47, Method: Compositional matrix adjust. Identities = 108/281 (38%), Positives = 179/281 (63%), Gaps = 1/281 (0%) Query: 12 LSQLLLGLVNGSFYAMLSLGLAVIFGLLNVINFAHGTLYMLGAFLAWMGLTYLGLNYWVM 71 L Q+ G+ G Y +L++GL+++FGLL +NF+HG ++LGA+L + +T LGL +W Sbjct: 5 LLQIANGVGIGMLYFLLAVGLSIVFGLLRFVNFSHGAFFLLGAYLCFQ-VTDLGLGFWWS 63 Query: 72 LIVAPILVGLFGIVIEKTMLRWLYKLDHLYGLLLTFGITLMIEGIFRSIYGVSGQPYSVP 131 L++AP++VGLF ++E+ +LR +Y L H + +L T G+ L+I+ S +G G + P Sbjct: 64 LLIAPLVVGLFAWIVERFVLRHVYALPHHFHILFTVGMALVIQEAVISTWGPLGNNVAPP 123 Query: 132 DLLQGATNLGFMVLPNYRAWVVVASLVICFATWFVIEKTRLGAYLRAGTENPKLVEAFGI 191 DLLQG G V P YR +V+ + V+ W+V+E TRLG+ +RAG+E+ ++V G+ Sbjct: 124 DLLQGVVMWGGFVYPKYRLFVIGLTAVMALLLWWVLEGTRLGSIVRAGSESTEMVSLLGL 183 Query: 192 NVPLMVTLTYGFGVALAAFAGVLAAPVIQVSPLMGSNLIITVFAVVVIGGMGSILGSIIT 251 N+ + +L + G A A AG LAAP+ P MG + F VVVIGG+GS G++I Sbjct: 184 NINRIFSLVFALGAATAGLAGALAAPIRGADPFMGVEALGIAFVVVVIGGLGSFWGALIG 243 Query: 252 GLVLGLIEGLTRVFYPELSATVVFIVMAIVLMVRPAGLFGK 292 G+++G+++ + + E + ++++ MA V+++RP GL G+ Sbjct: 244 GILVGIVQSVMSTVWSEGAHLMIYVAMAAVMLLRPNGLLGR 284 >gi|300312954|ref|YP_003777046.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 304 Score = 153 bits (386), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 101/297 (34%), Positives = 168/297 (56%), Gaps = 18/297 (6%) Query: 11 LLSQLLLGLVNGSFYAMLSLGLAVIFGLLNVINFAHGTLYMLGAFLAWMGLTYLGLNYWV 70 + Q+L L G +++ GL +IFG++N +N AHG+ +M+GA++A + ++W+ Sbjct: 5 IFEQVLNSLQFGVMLFLIASGLTLIFGVMNFVNLAHGSFFMIGAYVA-AVVAGASGSFWL 63 Query: 71 MLIVAPILVGLFGIVIEKTMLRWLYKLDHLYGLLLTFGITLMIEGIFRSIYGVSGQPYSV 130 L+VA G ++E + R LY+ DH+ +L TF L+ R I+G P ++ Sbjct: 64 GLLVALPATAALGFLLEWVVFRRLYQKDHMVQVLATFAFILIFNDGVRMIWGPQPLPLNM 123 Query: 131 PDLLQGATNLGFMVLPNYRAWVVVASLVICFATWFVIEKTRLGAYLRAGTENPKLVEAFG 190 P G NL + +YR V+ +V+ A +F+I +TR+G ++RAG N ++V A G Sbjct: 124 PAAFAGPVNLFGLPYSSYRLLVMAVGVVVAIALYFLINRTRIGMWVRAGANNLEMVTAMG 183 Query: 191 INVPLMVTLTYGFGVALAAFAGVLAAPVIQVSPLMGSNLIITVFAVVVIGGMGSILGSII 250 IN+ L+ L + FG+ LAA AG++ P++ V MG +++I F VVVIGG+GS+ G+ I Sbjct: 184 INIRLLFRLVFSFGLLLAALAGIMLGPLVAVQAGMGDDVLILAFVVVVIGGIGSVPGAAI 243 Query: 251 TGLVLGLIEGLTRVFYPELSA-----------------TVVFIVMAIVLMVRPAGLF 290 L++G+++ L R P+L A ++++VM VL RP+GLF Sbjct: 244 GALIVGVVDTLGRSLLPQLFALFLSADAAASVGPALGSMMIYLVMIAVLFWRPSGLF 300 >gi|300312237|ref|YP_003776329.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 293 Score = 138 bits (347), Expect = 6e-34, Method: Compositional matrix adjust. Identities = 93/286 (32%), Positives = 161/286 (56%), Gaps = 4/286 (1%) Query: 11 LLSQLLLGLVNGSFYAMLSLGLAVIFGLLNVINFAHGTLYMLGAFLAWMGLTYLGLNYWV 70 L QL+ L GS YA+ +LG ++FG+L VIN +HG ++MLG++ A + + + L WV Sbjct: 2 LEQQLINALSLGSVYALFALGFTLVFGVLGVINLSHGAIFMLGSYAALLLVEQMALPLWV 61 Query: 71 MLIVAPILVGLFGIVIEKTMLRWLYKLD--HLYGLLLTFGITLMIEGIFRSIYGVSGQPY 128 L++A + GL G++I+ +L+ L K + HL ++ T G+ +MI I + ++G + + Sbjct: 62 ALLLAMVATGLLGLIIDVLVLKPLRKRNAPHLIPMIATIGVAIMITNISQGLFGAENKRF 121 Query: 129 SVPDLLQGATNLGFMVLPNYRAWVVVASLVICFATWFVIEKTRLGAYLRAGTENPKLVEA 188 + + + +LG + + + ++V + V+ V+ KT+LG LRA E+PK Sbjct: 122 PQGTIPEESVSLGNLHITAVQVAIIVIAFVLMVVLLGVMRKTQLGRALRAIAESPKAAYL 181 Query: 189 FGINVPLMVTLT-YGFGVALAAFAGVLAAPVIQVSPLMGSNLIITVFAVVVIGGMGSILG 247 GINV + LT + A ++ +SP MG ++ AV+++GGMG I G Sbjct: 182 LGINVEGLFLLTSFAAAALGGAAGVLVGVSFNAISPFMGQPMLHKGIAVIILGGMGDIRG 241 Query: 248 SIITGLVLGLIEGLTRVF-YPELSATVVFIVMAIVLMVRPAGLFGK 292 ++I GL LG E LT + + V F ++ ++L+V+P+G+FGK Sbjct: 242 AMIGGLFLGFAEVLTVAYISSDFRDAVAFGLLFLILLVKPSGMFGK 287 >gi|300313286|ref|YP_003777378.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 288 Score = 130 bits (326), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 90/282 (31%), Positives = 154/282 (54%) Query: 12 LSQLLLGLVNGSFYAMLSLGLAVIFGLLNVINFAHGTLYMLGAFLAWMGLTYLGLNYWVM 71 L L G+ GS ++S+GL+V G++N IN AHG MLG ++ L +G+ + Sbjct: 6 LGVLFDGIAYGSLLFLISVGLSVTMGMMNFINLAHGAFAMLGGYVCVTLLNGMGVPFLAT 65 Query: 72 LIVAPILVGLFGIVIEKTMLRWLYKLDHLYGLLLTFGITLMIEGIFRSIYGVSGQPYSVP 131 L +A I + G+V+E+++ R LYK HL +L + G+T M +G + QP +P Sbjct: 66 LPLAFIAAAIVGLVLERSLYRRLYKASHLDQVLFSIGLTFMAVAGATWQWGPTQQPVVLP 125 Query: 132 DLLQGATNLGFMVLPNYRAWVVVASLVICFATWFVIEKTRLGAYLRAGTENPKLVEAFGI 191 D L+G +L + + YR +++ +++ A +IE+TR GA +RA +N GI Sbjct: 126 DWLRGQISLLGLEVGAYRVFLIGVVVIVTVALGLLIERTRFGAQIRASVDNQVAAAGLGI 185 Query: 192 NVPLMVTLTYGFGVALAAFAGVLAAPVIQVSPLMGSNLIITVFAVVVIGGMGSILGSIIT 251 NV + +LT+ G LA G L V+ + P ++ VV +GG G+I G ++ Sbjct: 186 NVSRVFSLTFALGSGLAGLGGGLGIDVLGLDPTFPVKYMVYFLLVVAVGGAGTIKGPLLA 245 Query: 252 GLVLGLIEGLTRVFYPELSATVVFIVMAIVLMVRPAGLFGKE 293 L+LG+ + + + PE+ A V++ +M ++L++ PAGL + Sbjct: 246 ALILGVFDVAGKYYVPEIGAFVIYGLMVVLLILFPAGLMRRR 287 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.330 0.147 0.443 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 697,985 Number of extensions: 24740 Number of successful extensions: 228 Number of sequences better than 1.0e-08: 24 Number of HSP's gapped: 197 Number of HSP's successfully gapped: 24 Length of query: 294 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 210 Effective length of database: 1,222,130 Effective search space: 256647300 Effective search space used: 256647300 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 130 (54.7 bits)