BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0300.p (112 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309403|ref|YP_003773495.1| nitrate/sulfonate/bicarbonate A... 152 8e-39 gi|300311839|ref|YP_003775931.1| nitrate/sulfonate/bicarbonate A... 81 2e-17 >gi|300309403|ref|YP_003773495.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 342 Score = 152 bits (383), Expect = 8e-39, Method: Compositional matrix adjust. Identities = 87/96 (90%), Positives = 89/96 (92%) Query: 1 MTTRLSSKGRLRRLFTTTLALSLLGAPLVARAEGQLRIAEQFGIVYLLLNVAQDQQLIEK 60 MT S K RLRRL T TLALSLLGAPL+ARAEGQLRIAEQFGIVYLLLNVAQDQQLIEK Sbjct: 1 MTLFPSFKERLRRLLTATLALSLLGAPLMARAEGQLRIAEQFGIVYLLLNVAQDQQLIEK 60 Query: 61 HGKAAGVDIKVEWLKLSGGSAVNDALLSGSVDIAGA 96 HGKAAGVDIKVEWLKLSGGSA+NDALLSGSVDIAGA Sbjct: 61 HGKAAGVDIKVEWLKLSGGSAINDALLSGSVDIAGA 96 >gi|300311839|ref|YP_003775931.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 341 Score = 80.9 bits (198), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 44/103 (42%), Positives = 67/103 (65%), Gaps = 5/103 (4%) Query: 12 RRLFTTTL---ALSLLGAPLVARAEGQ-LRIAEQFGIVYLLLNVAQDQQLIEKHGKAAGV 67 RR FT+ + AL+L G A AE Q +RI+ +G++YL L V +DQ L+EKH KA G+ Sbjct: 6 RRTFTSLMIASALALSGVSGTAHAEAQEVRISHGYGLLYLPLMVIRDQNLVEKHAKAMGL 65 Query: 68 -DIKVEWLKLSGGSAVNDALLSGSVDIAGAXACAASPRWATSR 109 ++K +W+ L GG+ +NDA+L+G++DIAG A W+ + Sbjct: 66 GEVKTKWVLLDGGNVINDAMLAGNLDIAGTGAPGFVTLWSKAH 108 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.131 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 265,813 Number of extensions: 8662 Number of successful extensions: 34 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 33 Number of HSP's successfully gapped: 2 Length of query: 112 Length of database: 1,619,870 Length adjustment: 73 Effective length of query: 39 Effective length of database: 1,274,215 Effective search space: 49694385 Effective search space used: 49694385 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 124 (52.4 bits)