BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0299.p (778 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309406|ref|YP_003773498.1| potassium uptake protein [Herba... 1129 0.0 gi|300314069|ref|YP_003778161.1| amino acid ABC transporter perm... 347 2e-96 gi|300313933|ref|YP_003778025.1| branched-chain amino acid ABC t... 80 5e-16 gi|300312953|ref|YP_003777045.1| amino acid ABC transporter perm... 76 8e-15 gi|300311771|ref|YP_003775863.1| branched-chain amino acid ABC t... 65 3e-11 >gi|300309406|ref|YP_003773498.1| potassium uptake protein [Herbaspirillum seropedicae SmR1] Length = 633 Score = 1129 bits (2921), Expect = 0.0, Method: Compositional matrix adjust. Identities = 578/595 (97%), Positives = 587/595 (98%) Query: 1 MGSSHTNSGLSLRSTKFHLITLAALGVVFGDIGTSPLYALKECFSPDHGIAFSPGAVLGI 60 M SSHTN LSLRS+KFHLITLAALGVVFGDIGTSPLYALKECFSPDHGIAFSPGAVLGI Sbjct: 1 MSSSHTNPSLSLRSSKFHLITLAALGVVFGDIGTSPLYALKECFSPDHGIAFSPGAVLGI 60 Query: 61 ISMLFWSISIVVTIKYVMFVLRADNNGEGGVLALMALSLRSAISGSPRAKLLMMLGVFGA 120 ISMLFWSISIVVTIKYVMFVLRADNNGEGGVLALMALSLRSA+SGSPRAKLLMMLGVFGA Sbjct: 61 ISMLFWSISIVVTIKYVMFVLRADNNGEGGVLALMALSLRSALSGSPRAKLLMMLGVFGA 120 Query: 121 CMFYGDVVITPAISVLSAVEGLEIAAPRTAHAVIPLTLVIVVILFLIQRHGTSVVGRFFG 180 CMFYGDVVITPAISVLSAVEGLEIAAP+TAHAVIPLTLVIVVILFLIQRHGTSVVGRFFG Sbjct: 121 CMFYGDVVITPAISVLSAVEGLEIAAPQTAHAVIPLTLVIVVILFLIQRHGTSVVGRFFG 180 Query: 181 PVMFLWFFSLALLGLYNIVKAPEVLVAINPYYAVHFMAEHSLQAFIVLGSVVLVLTGAEA 240 PVMFLWFFSLALLGLYNIVKAPEVLVAINPYYAVHFMAEHSLQAFIVLGSVVLVLTGAEA Sbjct: 181 PVMFLWFFSLALLGLYNIVKAPEVLVAINPYYAVHFMAEHSLQAFIVLGSVVLVLTGAEA 240 Query: 241 LYADMGHFGIKPIRYAWLFTVMPSLLINYFGQGANLIANPKAIANPFYLMIPEPLVLPMV 300 LYADMGHFGIKPIRYAWLFTVMPSLLINYFGQGANLIANP AI NPFYLMIP+PLVLPMV Sbjct: 241 LYADMGHFGIKPIRYAWLFTVMPSLLINYFGQGANLIANPDAIKNPFYLMIPDPLVLPMV 300 Query: 301 LLATCATVIASQAVISGAFSLTSQAILLGFVPRMRILHTSEDEQGQIYIPLINWMLLVLV 360 +LATCATVIASQAVISGAFSLTSQAILLGFVPRMRILHTSEDEQGQIYIPLINWMLLV+V Sbjct: 301 VLATCATVIASQAVISGAFSLTSQAILLGFVPRMRILHTSEDEQGQIYIPLINWMLLVVV 360 Query: 361 VAVVLAFKKSDNLAAAYGVAVTTTMVITTMLLGVVMRHIWKWRMTAVVVATTCFMVVDVA 420 VAVVLAFKKSDNLAAAYGVAVTTTMVITTMLLGVVMRHIWKWRM AVV A +CF+VVDVA Sbjct: 361 VAVVLAFKKSDNLAAAYGVAVTTTMVITTMLLGVVMRHIWKWRMPAVVAAISCFLVVDVA 420 Query: 421 FFAANLLKLTEGGWFPLVIGGAAFFLLMTWYSGRMLLRMRIKDDGIPIEPFIEGLLAHPP 480 FFAANLLKLTEGGWFPLVIGGAAFFLLMTWYSGRMLLRMRIKDDGIPIEPFIEGLLAHPP Sbjct: 421 FFAANLLKLTEGGWFPLVIGGAAFFLLMTWYSGRMLLRMRIKDDGIPIEPFIEGLLAHPP 480 Query: 481 HRVGGTAVFMTGNIATVPVALLHNLKHNRILHERVFFLKISIWDVPYVADSERLTMKDLG 540 HRVGGTAVFMTGNIATVPVALLHNLKHNRILHERVFFLKISIWDVPYVADSERLTMK+LG Sbjct: 481 HRVGGTAVFMTGNIATVPVALLHNLKHNRILHERVFFLKISIWDVPYVADSERLTMKELG 540 Query: 541 GDVYLLRAAFGFKEAPEVQKVMTLTEQQFGHHFDLMDTSFFLARDTVVPSKLLGM 595 GDVYLLRAAFGFKEAPEVQKVMTLTEQQFGHHFDLMDTSFFLARDTVVPSKLLGM Sbjct: 541 GDVYLLRAAFGFKEAPEVQKVMTLTEQQFGHHFDLMDTSFFLARDTVVPSKLLGM 595 >gi|300314069|ref|YP_003778161.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 326 Score = 347 bits (890), Expect = 2e-96, Method: Compositional matrix adjust. Identities = 177/189 (93%), Positives = 182/189 (96%) Query: 590 SKLLGMIDLSSDMTLYYVVLAIAVAGFALIVRTIHSPFGQVLKAIKENEPRAISLGYDVA 649 KLLG ++L SDMTLYYVVLAIAVAGFALIVRTIHSPFGQVLKAIKENEPRAISLGYDVA Sbjct: 138 GKLLGFLNLESDMTLYYVVLAIAVAGFALIVRTIHSPFGQVLKAIKENEPRAISLGYDVA 197 Query: 650 RYKLLAFVLSAGLSGLAGATKTLVLGFETLTDVHWSMSGLVVLMTLVGGLGTIVGPIVGA 709 RYKLLAFVLSAGLSGLAGATKTLVLGFETLTDVHWSMSGLVVLMTLVGGLGTI+GPIVGA Sbjct: 198 RYKLLAFVLSAGLSGLAGATKTLVLGFETLTDVHWSMSGLVVLMTLVGGLGTILGPIVGA 257 Query: 710 IIIIMLENKLGDIGAWLANVTSIDWFNTLGDSVTIVTGFIFIVCVLAFRKGIVGEILALA 769 +IIIMLENKLGDIGAWLA+VT IDWFNTLGDSVTIVTGFIFIVCVLAFRKGIVGEILAL Sbjct: 258 VIIIMLENKLGDIGAWLASVTHIDWFNTLGDSVTIVTGFIFIVCVLAFRKGIVGEILALT 317 Query: 770 GKGQAKGSH 778 KGQA GSH Sbjct: 318 RKGQASGSH 326 >gi|300313933|ref|YP_003778025.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 334 Score = 80.5 bits (197), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 49/136 (36%), Positives = 80/136 (58%), Gaps = 2/136 (1%) Query: 589 PSKLLGMIDLSSDMTL-YYVVLAIA-VAGFALIVRTIHSPFGQVLKAIKENEPRAISLGY 646 P L G+ L + + YY V+AI V F L+ R + S G+ L A++ENE RA +LGY Sbjct: 144 PLALFGLTLLPTTSSWQYYTVVAILFVLVFWLLQRVVDSVLGKTLLAVRENEARASALGY 203 Query: 647 DVARYKLLAFVLSAGLSGLAGATKTLVLGFETLTDVHWSMSGLVVLMTLVGGLGTIVGPI 706 DV KL AFV+S ++GLAG ++ G L+++ + S +++MT++GG G + + Sbjct: 204 DVRLLKLAAFVISGAVTGLAGGLHAMMTGVAPLSNIEYHTSEAILVMTVIGGTGNLFASV 263 Query: 707 VGAIIIIMLENKLGDI 722 +GA +++ N L + Sbjct: 264 LGASFYVLVGNWLSTL 279 >gi|300312953|ref|YP_003777045.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 317 Score = 76.3 bits (186), Expect = 8e-15, Method: Compositional matrix adjust. Identities = 49/116 (42%), Positives = 73/116 (62%), Gaps = 1/116 (0%) Query: 586 TVVPSKLLGM-IDLSSDMTLYYVVLAIAVAGFALIVRTIHSPFGQVLKAIKENEPRAISL 644 V LLG +D+S D Y+VVLA A F L+ R + S FG L AI++NE RA ++ Sbjct: 139 QVTARPLLGFGLDMSGDNAFYWVVLAWLAASFILLQRLLASRFGLALNAIRQNERRAQAI 198 Query: 645 GYDVARYKLLAFVLSAGLSGLAGATKTLVLGFETLTDVHWSMSGLVVLMTLVGGLG 700 GY RYKL AFV++A ++G+AG+ + F + +HWS SG++++M +GG+G Sbjct: 199 GYPALRYKLAAFVIAAVIAGIAGSLIGMSNLFASPKLLHWSQSGILLVMVALGGVG 254 >gi|300311771|ref|YP_003775863.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 628 Score = 64.7 bits (156), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 42/122 (34%), Positives = 66/122 (54%), Gaps = 4/122 (3%) Query: 598 LSSDMTLYYVVLAIAVAGFALIVRTIHSPFGQVLKAIKENEPRAISLGYDVARYKLLAFV 657 L+S + YY+ L + G L+ R IH+PFG L+A ++ RA +LG DV + F Sbjct: 467 LASPLAYYYLALLCSALGVYLLRRVIHAPFGLALRAARDASGRAQALGMDVRALQWGGFA 526 Query: 658 LSAGLSGLAGATKTLVLG--FETLTDVHWSMSGLVVLMTLVGGLGTIVGPIVGAIIIIML 715 L+ GLAG G + V+ S+ GLV M L+GG+ ++ GP++GA + L Sbjct: 527 LAGLFCGLAGVLFAFSKGSISPEVAGVNRSVDGLV--MVLLGGVQSLAGPLLGASVFTWL 584 Query: 716 EN 717 ++ Sbjct: 585 QD 586 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.142 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,214,629 Number of extensions: 88550 Number of successful extensions: 363 Number of sequences better than 1.0e-08: 21 Number of HSP's gapped: 365 Number of HSP's successfully gapped: 21 Length of query: 778 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 685 Effective length of database: 1,179,515 Effective search space: 807967775 Effective search space used: 807967775 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 134 (56.2 bits)