BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0291.p (266 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309409|ref|YP_003773501.1| glutamine ABC transporter perip... 469 e-133 gi|300313610|ref|YP_003777702.1| amino acid ABC transporter/sign... 204 5e-54 gi|300312810|ref|YP_003776902.1| glutamine ABC transporter/signa... 121 6e-29 gi|300311472|ref|YP_003775564.1| amino acid ABC transporter/sign... 100 1e-22 gi|300310494|ref|YP_003774586.1| amino acid ABC transporter/sign... 84 1e-17 >gi|300309409|ref|YP_003773501.1| glutamine ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 259 Score = 469 bits (1206), Expect = e-133, Method: Compositional matrix adjust. Identities = 230/239 (96%), Positives = 236/239 (98%) Query: 28 GSTFAADQTYVVGAGGTYRPFEFETPKKELVGFDIDLIKAISQASNFKIQLVSTPWEGIF 87 G+ AADQTYVVGAGGTYRPFEFETPKKELVGFDIDLIKAISQASNFKIQLVSTPWEGIF Sbjct: 21 GTALAADQTYVVGAGGTYRPFEFETPKKELVGFDIDLIKAISQASNFKIQLVSTPWEGIF 80 Query: 88 ATLGQGDRDIIISGITITDKRAQMVDFSLPYFPAEQVIITNADAKIASLADLKKTQVAVV 147 ATLGQGDRDIIISGITITDKRAQMVDFSLPYFPAEQVIITNADAKI SL+DLKKTQVAVV Sbjct: 81 ATLGQGDRDIIISGITITDKRAQMVDFSLPYFPAEQVIITNADAKITSLSDLKKTQVAVV 140 Query: 148 NSTAGDIVVSEELGKSSTAIHRFDNTVLMLEELYRGGVDAAVGDVGVIKFYIKSHPEKKF 207 NSTAGDIVVSEELGK+STAIHRFDNTVLMLEELYRGGVDAAVGDVGVIKFYIKSHPEKKF Sbjct: 141 NSTAGDIVVSEELGKASTAIHRFDNTVLMLEELYRGGVDAAVGDVGVIKFYIKSHPEKKF 200 Query: 208 KVVGDTKFVRQYFGIAVKKGNKELLDKINAGLKQIVADGTYAKIYKTWFDGEVPTLPLK 266 KVVGD+KFVRQYFGIAVKKGNKELLDKINAGLK+IVADGTYAKIYKTWFDG+VPTLPLK Sbjct: 201 KVVGDSKFVRQYFGIAVKKGNKELLDKINAGLKKIVADGTYAKIYKTWFDGDVPTLPLK 259 >gi|300313610|ref|YP_003777702.1| amino acid ABC transporter/signal transduction system periplasmic protein/domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 271 Score = 204 bits (520), Expect = 5e-54, Method: Compositional matrix adjust. Identities = 105/223 (47%), Positives = 148/223 (66%), Gaps = 1/223 (0%) Query: 35 QTYVVGAGGTYRPFEFETPKKELVGFDIDLIKAISQASNFKIQLVSTPWEGIFATLGQGD 94 + YVVG Y PF E +K++VGFDID++KA+++ +++ V TP+EG F L QGD Sbjct: 47 RVYVVGVESAYAPFSSENEQKDVVGFDIDIMKALAKKIGIEVKFVPTPFEGFFNFLAQGD 106 Query: 95 RDIIISGITITDKRAQMVDFSLPYFPAEQVI-ITNADAKIASLADLKKTQVAVVNSTAGD 153 RD++IS ITITD+R + V FS PYF A Q I + AD K++ + DLK V ++T+GD Sbjct: 107 RDLLISAITITDERKKSVAFSEPYFVATQTIALPAADTKVSKMEDLKPLTVGTQSATSGD 166 Query: 154 IVVSEELGKSSTAIHRFDNTVLMLEELYRGGVDAAVGDVGVIKFYIKSHPEKKFKVVGDT 213 +V + LGK+S I RFD+T L L+EL GGVDA V D V+K YI ++P K + V D Sbjct: 167 ELVQQVLGKNSAKIKRFDSTPLALKELESGGVDAVVADEPVVKNYIANNPNSKLRTVTDP 226 Query: 214 KFVRQYFGIAVKKGNKELLDKINAGLKQIVADGTYAKIYKTWF 256 F ++ +GIAV+K + ELL KIN GL ++ ADG++A I +F Sbjct: 227 SFPKEDYGIAVRKDDPELLAKINKGLAEMKADGSFAAISAQYF 269 >gi|300312810|ref|YP_003776902.1| glutamine ABC transporter/signal transduction system periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 249 Score = 121 bits (304), Expect = 6e-29, Method: Compositional matrix adjust. Identities = 80/227 (35%), Positives = 121/227 (53%), Gaps = 5/227 (2%) Query: 35 QTYVVGAGGTYRPFEFETPKKELVGFDIDLIKAISQASNFKIQLVSTPWEGIFATLGQGD 94 +T VV + PFEF+ + + VGFDIDL AI++ +L + GI L + Sbjct: 26 ETLVVACDTAFVPFEFKD-RNQYVGFDIDLWDAIAKDIGVTYKLQPMDFNGIIPALQTKN 84 Query: 95 RDIIISGITITDKRAQMVDFSLPYFPAEQVIITNADAKIASLADLKKTQVAVVNSTAGDI 154 D+ ++GITI D+R +++DFS Y+ + ++ + I S+ADL +AV + T+ Sbjct: 85 VDVALAGITIKDERKKVIDFSDGYYDSGFSLMVPVGSSIKSVADLAGKALAVKSGTSAFD 144 Query: 155 VVSEELGKSSTAIHRFDNTVLMLEELYRGGVDAAVGDVGVIKFYIKSHPEKKFKVVGDTK 214 +T +H+F N EL G VDAA+ D + +YI + + + KVVG Sbjct: 145 YAKANF--KATELHQFPNIDNAYLELQTGRVDAAMHDTPNVLYYIATAGKGRAKVVGTQM 202 Query: 215 FVRQYFGIAVKKGNKELLDKINAGLKQIVADGTYAKIYKTWFDGEVP 261 QY GI KG+ L+ K+NA L +I ADG YA+IY+ WF E P Sbjct: 203 MAHQY-GIGFPKGS-PLVPKVNAALAKIKADGRYAEIYRKWFGAEPP 247 >gi|300311472|ref|YP_003775564.1| amino acid ABC transporter/signal transduction system periplasmic protein/domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 267 Score = 100 bits (250), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 76/236 (32%), Positives = 112/236 (47%), Gaps = 16/236 (6%) Query: 36 TYVVGAGGTYRPFEFETPKK-ELVGFDIDLIKAISQASNFKIQLVSTPWEGIFATLGQGD 94 T V G Y PF F+ PK EL GF++D+ K ++ K +T W GI A LG G Sbjct: 38 TLKVALEGNYPPFNFKDPKTGELTGFEVDVAKLLAAKLGVKPVFTTTEWSGILAGLGAGK 97 Query: 95 RDIIISGITITDKRAQMVDFSLPYFPAEQVIITNADAK--IASLADLKKTQVAVVNSTAG 152 D+II+ + ITD+R + DFS PY + +I D K SL DLK ++ + T Sbjct: 98 YDVIINQVGITDERQKAFDFSDPYTLSSAQLIVRKDEKREFKSLEDLKGKKLGLGQGTN- 156 Query: 153 DIVVSEELGKSSTAI--HRFDNTVLMLEELYRGGVDAAVGDVGVIKFYIKSH--PEKKFK 208 E+ K+ I + + L +L G +DAA+ D ++ + +KS P K Sbjct: 157 ----FEQKAKAVPGIDVRTYPGSPEYLADLAAGRIDAALNDSLLVGYILKSTNLPLKAGS 212 Query: 209 VVGDTKFVRQYFGIAVKKGNKELLDKINAGLKQIVADGTYAKIYKTWFDGEVPTLP 264 +G + GI +KGN E +N L I ADG++ + WF +V P Sbjct: 213 PIGAVDKI----GIPFRKGNPEFKAALNKALADIKADGSFKAASEKWFGIDVSQPP 264 >gi|300310494|ref|YP_003774586.1| amino acid ABC transporter/signal transduction system periplasmic protein/domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 258 Score = 84.3 bits (207), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 61/238 (25%), Positives = 117/238 (49%), Gaps = 12/238 (5%) Query: 23 GXVPAGSTFAADQTYVVGAGGTYRPFEF-ETPKKELVGFDIDLIKAISQASNFKIQLVST 81 G PA A +Y VGA T PF F + +L G +D + A+ + F + + T Sbjct: 25 GSAPA----MAQASYNVGATSTGVPFTFLDIKTNKLQGMMVDTVTAVGNKAGFSVNIQQT 80 Query: 82 PWEGIFATLGQGDRDIIISGITITDKRAQMVDFSLPYFPAEQVIITNA-DAKIASLADLK 140 P+ + +L DII + + T+ RAQ+VDFS P + + + NA D K S +L Sbjct: 81 PFAALIPSLTGNKIDIIAAAMLKTEARAQIVDFSDPVYSYGEGLFVNAQDGK--SYKNLD 138 Query: 141 KTQVAVVNSTAGDIVVSEELGKSS--TAIHRFDNTVLMLEELYRGGVDAAVGDVGVIKFY 198 + +V + G + V + L K + +D+ M+ ++ G + A GD ++ + Sbjct: 139 DLKGEIVGAQVGTVFV-DALNKKGGFKEVKTYDSVADMMRDVALGRIKAGFGDRPIVAYQ 197 Query: 199 IKSHPEKKFKVVGDTK-FVRQYFGIAVKKGNKELLDKINAGLKQIVADGTYAKIYKTW 255 + + ++V + + + + ++KG+ +L++++N + ++ ADGT A+I + W Sbjct: 198 LAQGANPQVRLVKEYQPTLMGDVCLVIRKGDPKLMERLNKAIAELKADGTLARIVEQW 255 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.136 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 742,976 Number of extensions: 27833 Number of successful extensions: 100 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 89 Number of HSP's successfully gapped: 14 Length of query: 266 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 182 Effective length of database: 1,222,130 Effective search space: 222427660 Effective search space used: 222427660 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)