BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0022.0260.p
         (322 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300309433|ref|YP_003773525.1| glutathione synthetase [Herbasp...   614   e-177

>gi|300309433|ref|YP_003773525.1| glutathione synthetase
           [Herbaspirillum seropedicae SmR1]
          Length = 316

 Score =  614 bits (1583), Expect = e-177,   Method: Compositional matrix adjust.
 Identities = 297/314 (94%), Positives = 306/314 (97%)

Query: 1   MKIAFLADPLDTFKTYKDSTFAMMREAARRGHSIYAFEQGDLVLENGLVTANAFKITLTG 60
           MKIAFLADPLDTFKTYKDSTFAMMREAARRGH +YAFEQ DLVLE+GLVTANA K+TLTG
Sbjct: 1   MKIAFLADPLDTFKTYKDSTFAMMREAARRGHVLYAFEQADLVLESGLVTANACKLTLTG 60

Query: 61  EADDWYRADKPASSFLSEFDAVIVRKDPPFDMEYIYTTYLLELAEKQGARVFNKPAAIRS 120
           +ADDWYR DKP SSFLSEFDAVIVRKDPPFDMEYIYTTYLLELAEKQGARVFNKPAAIRS
Sbjct: 61  DADDWYRTDKPVSSFLSEFDAVIVRKDPPFDMEYIYTTYLLELAEKQGARVFNKPAAIRS 120

Query: 121 HNEKLAIAQFPQYTAPTLVTRDAKRLRAFHDEHQDVILKPLDGMGGMGIFRVKADGMNLG 180
           HNEKLAIAQFPQYTAPTLVTRDAKRLRAFH EHQDVILKPLDGMGGMGIFRVKADGMNLG
Sbjct: 121 HNEKLAIAQFPQYTAPTLVTRDAKRLRAFHAEHQDVILKPLDGMGGMGIFRVKADGMNLG 180

Query: 181 SIIETLTDNGQRTIMAQRFIPQISAGDKRILLIGGKVVPNVLARIPQGNEVRGNLAAGGL 240
           SIIETLTDNGQRTIMAQRFIPQISAGDKRILLIGGKVVPNVLARIPQGNEVRGNLAAGGL
Sbjct: 181 SIIETLTDNGQRTIMAQRFIPQISAGDKRILLIGGKVVPNVLARIPQGNEVRGNLAAGGL 240

Query: 241 GKAQPASARDIEIGEALGPILYQRGLLLVGLDAIGDYLTEINVTSPTCFQEITQQTGFDV 300
           G+AQPASARDIEIGEALGP+L++RGLLLVGLDAIGDYLTEINVTSPTCFQEITQQTGFDV
Sbjct: 241 GRAQPASARDIEIGEALGPVLHERGLLLVGLDAIGDYLTEINVTSPTCFQEITQQTGFDV 300

Query: 301 AAMFVDALEAAAGA 314
           AAMFVDALEA  G+
Sbjct: 301 AAMFVDALEAEVGS 314


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.320    0.137    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,009,350
Number of extensions: 44779
Number of successful extensions: 127
Number of sequences better than 1.0e-08: 1
Number of HSP's gapped: 127
Number of HSP's successfully gapped: 1
Length of query: 322
Length of database: 1,619,870
Length adjustment: 85
Effective length of query: 237
Effective length of database: 1,217,395
Effective search space: 288522615
Effective search space used: 288522615
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 130 (54.7 bits)