BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0252.p (396 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309454|ref|YP_003773546.1| GTPase translation elongation f... 806 0.0 gi|300309440|ref|YP_003773532.1| EF-Tu elongation factor protein... 806 0.0 gi|300312403|ref|YP_003776495.1| sulfate adenylyltransferase sub... 82 7e-17 gi|300312895|ref|YP_003776987.1| GTP-binding elongation factor p... 76 5e-15 gi|300312898|ref|YP_003776990.1| translation initiation factor I... 65 1e-11 >gi|300309454|ref|YP_003773546.1| GTPase translation elongation factor TU [Herbaspirillum seropedicae SmR1] Length = 396 Score = 806 bits (2082), Expect = 0.0, Method: Compositional matrix adjust. Identities = 396/396 (100%), Positives = 396/396 (100%) Query: 1 MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLSKKFGGEAKAYDQIDAAPEEKARG 60 MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLSKKFGGEAKAYDQIDAAPEEKARG Sbjct: 1 MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLSKKFGGEAKAYDQIDAAPEEKARG 60 Query: 61 ITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI 120 ITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI Sbjct: 61 ITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI 120 Query: 121 LLSRQVGVPYIIVFLNKADMVDDAELLELVEMEVRELLSKYEFPGDDLPIVKGSAKLALE 180 LLSRQVGVPYIIVFLNKADMVDDAELLELVEMEVRELLSKYEFPGDDLPIVKGSAKLALE Sbjct: 121 LLSRQVGVPYIIVFLNKADMVDDAELLELVEMEVRELLSKYEFPGDDLPIVKGSAKLALE 180 Query: 181 GDTGPLGEQAIMALAEALDTYIPTPERAVDGTFLMPVEDVFSISGRGTVVTGRVERGIIK 240 GDTGPLGEQAIMALAEALDTYIPTPERAVDGTFLMPVEDVFSISGRGTVVTGRVERGIIK Sbjct: 181 GDTGPLGEQAIMALAEALDTYIPTPERAVDGTFLMPVEDVFSISGRGTVVTGRVERGIIK 240 Query: 241 VGEEIEIVGIADTQKTTCTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVQRGQVLAKPGS 300 VGEEIEIVGIADTQKTTCTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVQRGQVLAKPGS Sbjct: 241 VGEEIEIVGIADTQKTTCTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVQRGQVLAKPGS 300 Query: 301 IKPHKHFTGEIYVLSKDEGGRHTPFFNNYRPQFYFRTTDVTGAIELPKDKEMVMPGDNVS 360 IKPHKHFTGEIYVLSKDEGGRHTPFFNNYRPQFYFRTTDVTGAIELPKDKEMVMPGDNVS Sbjct: 301 IKPHKHFTGEIYVLSKDEGGRHTPFFNNYRPQFYFRTTDVTGAIELPKDKEMVMPGDNVS 360 Query: 361 ITVQLINPIAMEEGLRFAIREGGRTVGAGVVAKIFD 396 ITVQLINPIAMEEGLRFAIREGGRTVGAGVVAKIFD Sbjct: 361 ITVQLINPIAMEEGLRFAIREGGRTVGAGVVAKIFD 396 >gi|300309440|ref|YP_003773532.1| EF-Tu elongation factor protein [Herbaspirillum seropedicae SmR1] Length = 396 Score = 806 bits (2082), Expect = 0.0, Method: Compositional matrix adjust. Identities = 396/396 (100%), Positives = 396/396 (100%) Query: 1 MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLSKKFGGEAKAYDQIDAAPEEKARG 60 MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLSKKFGGEAKAYDQIDAAPEEKARG Sbjct: 1 MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLSKKFGGEAKAYDQIDAAPEEKARG 60 Query: 61 ITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI 120 ITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI Sbjct: 61 ITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI 120 Query: 121 LLSRQVGVPYIIVFLNKADMVDDAELLELVEMEVRELLSKYEFPGDDLPIVKGSAKLALE 180 LLSRQVGVPYIIVFLNKADMVDDAELLELVEMEVRELLSKYEFPGDDLPIVKGSAKLALE Sbjct: 121 LLSRQVGVPYIIVFLNKADMVDDAELLELVEMEVRELLSKYEFPGDDLPIVKGSAKLALE 180 Query: 181 GDTGPLGEQAIMALAEALDTYIPTPERAVDGTFLMPVEDVFSISGRGTVVTGRVERGIIK 240 GDTGPLGEQAIMALAEALDTYIPTPERAVDGTFLMPVEDVFSISGRGTVVTGRVERGIIK Sbjct: 181 GDTGPLGEQAIMALAEALDTYIPTPERAVDGTFLMPVEDVFSISGRGTVVTGRVERGIIK 240 Query: 241 VGEEIEIVGIADTQKTTCTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVQRGQVLAKPGS 300 VGEEIEIVGIADTQKTTCTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVQRGQVLAKPGS Sbjct: 241 VGEEIEIVGIADTQKTTCTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVQRGQVLAKPGS 300 Query: 301 IKPHKHFTGEIYVLSKDEGGRHTPFFNNYRPQFYFRTTDVTGAIELPKDKEMVMPGDNVS 360 IKPHKHFTGEIYVLSKDEGGRHTPFFNNYRPQFYFRTTDVTGAIELPKDKEMVMPGDNVS Sbjct: 301 IKPHKHFTGEIYVLSKDEGGRHTPFFNNYRPQFYFRTTDVTGAIELPKDKEMVMPGDNVS 360 Query: 361 ITVQLINPIAMEEGLRFAIREGGRTVGAGVVAKIFD 396 ITVQLINPIAMEEGLRFAIREGGRTVGAGVVAKIFD Sbjct: 361 ITVQLINPIAMEEGLRFAIREGGRTVGAGVVAKIFD 396 >gi|300312403|ref|YP_003776495.1| sulfate adenylyltransferase subunit 1 protein [Herbaspirillum seropedicae SmR1] Length = 443 Score = 82.0 bits (201), Expect = 7e-17, Method: Compositional matrix adjust. Identities = 109/436 (25%), Positives = 187/436 (42%), Gaps = 83/436 (19%) Query: 17 TIGHVDHGKTTLTA---------------AIATVLSKKFGGEAKAYDQI-DAAPEEKARG 60 T G VD GK+TL AI+ K+ G+ + D E+ +G Sbjct: 27 TAGSVDDGKSTLIGRLLFDSKGIFADQLDAISRAKHKRTVGDTVDLSLLTDGLEAEREQG 86 Query: 61 ITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAA------DGP-- 112 ITI+ A+ + T R + D PGH Y +NM+TGA+ D I++ + DG Sbjct: 87 ITIDVAYRYFATPKRKFIIADTPGHEQYTRNMVTGASTADAVIILVDVSKVKLGEDGSVE 146 Query: 113 -MPQTREHILLSRQVGVPYIIVFLNKADMVD-DAELLELVEMEVRELLSKY--------- 161 + QT+ H ++ + + ++IV +NK D+VD D + + + ++ + Sbjct: 147 LLTQTKRHSTIAHLLQIQHVIVAVNKMDLVDYDQTVYDRIVGAYQQFAQQLGLQDVHAIP 206 Query: 162 --EFPGDDLPIVKGSAKLALEGDTGPLGEQAIMALAEALDTYIPTPERAVDGTFLMPVED 219 GD+ +V+ SA++ GP ++ L E+L Y E A + F PV+ Sbjct: 207 LSALAGDN--VVEASARMPWY--QGP----TLITLLESLSVY----EDAHEEAFRFPVQL 254 Query: 220 VFSISG------RGTVVTGRVERGIIKVGEEIEIVGIADTQKTTCTGVEMFRKLLDQGQA 273 V +G RG + GR+E G + G+ ++V + Q T ++ L+ A Sbjct: 255 VARHNGHEANDFRGYM--GRIEAGKVARGD--KLVVLPSGQSATVKDIQTLDGSLESASA 310 Query: 274 GDNVGVLLRGTKREDVQRGQVL---AKPGSIKPHKHFTGEIYVLSKDE-GGRHTPFFNNY 329 G +V +LL + D+ RG +L A+P +I K ++ LS++ R + + Sbjct: 311 GQSVTILL--DEYLDISRGDLLASAAQPSTIL--KTVNADVCWLSEEPLDLRRKYWLKHT 366 Query: 330 RPQFYFRTTDVTGAIELPKDK----EMVMPGDNVSITVQLINPIAMEEGLRFAIREGG-- 383 Q R V +++ + E + D I+V + PIA + AIR G Sbjct: 367 TKQVAARVAKVDTLLDINTQERRPAETLKLNDIARISVNVQQPIAADA--YQAIRATGAF 424 Query: 384 --------RTVGAGVV 391 +TV AG++ Sbjct: 425 ILIDEVSHQTVAAGMI 440 >gi|300312895|ref|YP_003776987.1| GTP-binding elongation factor protein [Herbaspirillum seropedicae SmR1] Length = 627 Score = 76.3 bits (186), Expect = 5e-15, Method: Compositional matrix adjust. Identities = 88/297 (29%), Positives = 134/297 (45%), Gaps = 35/297 (11%) Query: 20 HVDHGKTTLTAAIATVLSKKFGGEAKAYDQI-DAAPEEKARGITINTAHVEYETANRHYA 78 HVDHGKTTL + S F + +++ D+ EK RGITI + + E H Sbjct: 30 HVDHGKTTLVDQLLR-QSGTFRDNQQVDNRVMDSNDIEKERGITILSKNCAVEYKGTHIN 88 Query: 79 HVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLSRQVGVPYIIVFLNKA 138 VD PGHAD+ + + +D +L+ A +GPMPQTR + +G+ I+V LNK Sbjct: 89 IVDTPGHADFGGEVERVLSMVDSVLLLVDAQEGPMPQTRFVTRKALALGLKPIVV-LNKI 147 Query: 139 DMVD-DAELLELVEMEVRELLSKYEFPGDDLPIV-----KGSAKL---ALEGDTGPLGEQ 189 D AE E+ + L E D P++ G A L A EG+ P Sbjct: 148 DRPGARAEWAINATFELFDKLGATE-EQLDFPVIYASGLNGYASLDPEAREGNMEP---- 202 Query: 190 AIMALAEALDTYIPTPERAVDGTFLMPVEDVFSISGRGTVVTGRVERGIIKVGEEIEIVG 249 L +A+ Y+P + D + + + S G + GR+ G +K G+++ + Sbjct: 203 ----LFDAILKYVPARKDDPDAPLQLQITSLEYSSYVGKIGVGRILAGRVKGGQDVVWMN 258 Query: 250 IADTQKT--------TCTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVQRGQVLAKP 298 D + T T G+E R L+D+ AGD VL+ G E++ G + P Sbjct: 259 GPDDKPTKARINQVLTFKGLE--RVLVDEALAGDI--VLINGI--EEIGIGSTICSP 309 >gi|300312898|ref|YP_003776990.1| translation initiation factor IF-2 [Herbaspirillum seropedicae SmR1] Length = 954 Score = 64.7 bits (156), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 77/232 (33%), Positives = 102/232 (43%), Gaps = 33/232 (14%) Query: 15 VGTIGHVDHGKTTLTAAIATVLSKKFGGEAKAYDQIDAAPEEKARGITINTAHVEYETAN 74 V +GHVDHGKT+L I +K GEA GIT + ET Sbjct: 459 VTVMGHVDHGKTSLLDYIRR--TKVASGEAG--------------GITQHIGAYHVETPR 502 Query: 75 RHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLSRQVGVPYIIVF 134 +D PGH + GA D ILV +A DG MPQT+E I ++ GVP ++V Sbjct: 503 GMITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTKEAIAHAKAGGVP-LVVA 561 Query: 135 LNKAD---MVDDAELLELVEMEVRELLSKYEFPGDDLPIVKGSAKLALEGDTGPLGEQAI 191 +NK D D EL+ EV + +Y G D P + SAK EG L + + Sbjct: 562 INKIDKPGANPDRVKQELIAEEV--VPEEY---GGDAPFIPVSAKTG-EGIDSLL--ENV 613 Query: 192 MALAEALDTYIPTPERAVDGTFLMPVEDVFSISGRGTVVTGRVERGIIKVGE 243 + AE L+ P E G + D GRG V T V+ G +K G+ Sbjct: 614 LLQAEVLELKAPV-EVPARGLVIEAKLD----KGRGPVATILVQSGTLKRGD 660 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.137 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,151,181 Number of extensions: 44617 Number of successful extensions: 134 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 127 Number of HSP's successfully gapped: 9 Length of query: 396 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 309 Effective length of database: 1,207,925 Effective search space: 373248825 Effective search space used: 373248825 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)