BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0242.p (179 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309724|ref|YP_003773816.1| transmembrane protein [Herbaspi... 285 2e-78 >gi|300309724|ref|YP_003773816.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 179 Score = 285 bits (728), Expect = 2e-78, Method: Compositional matrix adjust. Identities = 150/177 (84%), Positives = 168/177 (94%) Query: 1 VLHSIFTMIVNAVASILAGVLLLRFWMQVVRVRPPQSVGQFVYQLSDWLVLPLRRVMPGG 60 +LHSIF +IVNAVASILAGV+LLRFWMQVVRVRPPQSVGQFVYQLSDWLV PLRRV+PGG Sbjct: 1 MLHSIFMLIVNAVASILAGVMLLRFWMQVVRVRPPQSVGQFVYQLSDWLVKPLRRVLPGG 60 Query: 61 RHDWASLLGAFLIVLLSIIAILGFQSGFDFKLIFLVALERFFEWICYGFMATLIIEAVFS 120 +DWASLLGAFLIV+L+ +A +G QSGFDFK+IFL++LERF EWICYGFMATLIIEA+FS Sbjct: 61 AYDWASLLGAFLIVVLATVAAVGLQSGFDFKIIFLLSLERFVEWICYGFMATLIIEAIFS 120 Query: 121 WVNPHAPLAPFVRALNDPLLRPLRKIIPPLGGVDLSPLVLLILLQIAVRVVSGLLFG 177 WVNP+APLAPFVRALN+PLLRPLR++IPPLGGVDLSPLVLLILLQIAVRV+S LLF Sbjct: 121 WVNPYAPLAPFVRALNEPLLRPLRRVIPPLGGVDLSPLVLLILLQIAVRVISTLLFA 177 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.335 0.150 0.477 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 492,043 Number of extensions: 18745 Number of successful extensions: 104 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 102 Number of HSP's successfully gapped: 1 Length of query: 179 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 100 Effective length of database: 1,245,805 Effective search space: 124580500 Effective search space used: 124580500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 39 (21.6 bits) S2: 127 (53.5 bits)