BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0232.p (325 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309715|ref|YP_003773807.1| nitrate/sulfonate/bicarbonate A... 639 0.0 gi|300312620|ref|YP_003776712.1| ABC transporter periplasmic pro... 68 8e-13 gi|300313870|ref|YP_003777962.1| nitrate/sulfonate/bicarbonate A... 66 3e-12 gi|300310977|ref|YP_003775069.1| alkanesulfonates ABC transporte... 61 2e-10 gi|300309986|ref|YP_003774078.1| nitrate/sulfonate/bicarbonate A... 59 5e-10 >gi|300309715|ref|YP_003773807.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 319 Score = 639 bits (1647), Expect = 0.0, Method: Compositional matrix adjust. Identities = 312/318 (98%), Positives = 314/318 (98%) Query: 8 QKQWIALALLACAGFSGASHAADDVIRLGNLKFAHYGAVSYIKEIAPKCGIKVDEKVFAK 67 QK+ IALALLA AGFS ASHAADDVIRLGNLKFAHYGAVSYIKEIAPKCGIKVDEKVFAK Sbjct: 2 QKKLIALALLASAGFSAASHAADDVIRLGNLKFAHYGAVSYIKEIAPKCGIKVDEKVFAK 61 Query: 68 GPDVMQGILAGELDVGTTASEAAISGRANGAPIYVVAGFAKGGARLVAGKDSGIKSVKDL 127 GPDVMQGILAGELDVGTTASEAAISGRANGAPIYVVAGFAKGGARLVAGKDSGIKSVKDL Sbjct: 62 GPDVMQGILAGELDVGTTASEAAISGRANGAPIYVVAGFAKGGARLVAGKDSGIKSVKDL 121 Query: 128 KGKKVGVTRGGIHEVLLDAELGQNGLSAKDVTIVYLAFADLNQALLGKNIDAMMQSEPQS 187 KGKKVGVTRGGIHEVLLDAELGQNGLSAKDVTIVYLAFADLNQALLGKNIDAMMQSEPQS Sbjct: 122 KGKKVGVTRGGIHEVLLDAELGQNGLSAKDVTIVYLAFADLNQALLGKNIDAMMQSEPQS 181 Query: 188 SQAINKGFGVEVMKPYDTPIGEPYRTMVMTEKFYTEKRPLAEKFMRCFLEATKTFIDKPE 247 SQAINKGFGVEVMKPYDTPIGEPYRTMVMTEKFY EKRPLAEKFMRCFLEATKTFIDKPE Sbjct: 182 SQAINKGFGVEVMKPYDTPIGEPYRTMVMTEKFYNEKRPLAEKFMRCFLEATKTFIDKPE 241 Query: 248 VAEKYVREVIFKNQISKEDFYDAIGNSPYAYDITAEHIQVTTDTMAKYGTGKMAKPPVAK 307 VAEKYVREVIFKNQISKEDFYDAIGNSPYAYDITAEHIQ+TTDTMAKYGTGKMAKPPVAK Sbjct: 242 VAEKYVREVIFKNQISKEDFYDAIGNSPYAYDITAEHIQITTDTMAKYGTGKMAKPPVAK 301 Query: 308 DWVKTDLLEQAKKSMNLK 325 DWVKTDLLEQAKKSMNLK Sbjct: 302 DWVKTDLLEQAKKSMNLK 319 >gi|300312620|ref|YP_003776712.1| ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 333 Score = 68.2 bits (165), Expect = 8e-13, Method: Compositional matrix adjust. Identities = 84/301 (27%), Positives = 133/301 (44%), Gaps = 31/301 (10%) Query: 22 FSGASHAADDVIRLGNLKFAH--YGAVSYIKEIAPKCGIKVDEKVFAKGPDVMQGILAGE 79 FSG + A + ++G L+ +Y + K G+ V+ F G D+++ I+ G+ Sbjct: 18 FSGNAFALEK-FKIGYLRVMDDAQAMAAYEAGLYKKYGLDVELIEFKSGTDLVKAIVGGQ 76 Query: 80 LDVGTTASEAAISGRANGAPIYVVAGFAKGGARLVAGKDSGIKSVKDLKGKKVGVTRGG- 138 LD G A++ + GA + VV G G LVA +DSGI V DLKGK + G Sbjct: 77 LDSGVFGFSNAVAWASKGADLKVVGGAQLGYHALVAREDSGINKVADLKGKTLASQAEGS 136 Query: 139 -IHEVLLDAELGQNGLSAKDVTIVYLAFADLNQALLGKNIDAMMQSEPQSSQAINKGFGV 197 VL L G+ A DV ++ ++ Q+L+GK +DA EPQ+ A Sbjct: 137 TADTVLKGVVLRDAGIKADDVNVMGVSPQVAVQSLVGKRVDAAFLFEPQARIA------- 189 Query: 198 EVMKPYDT--PIGE--PYRTMVMTEKFYT--EKRPLAEKFMRCFLEATKTFIDKPEVAEK 251 +++ P IGE P+ MV+ T +KR K + +A KP A K Sbjct: 190 QLIAPVKQVYEIGEVWPFPCMVVITSGETLKKKREAVWKSLDAQRDAITLLQKKPAEASK 249 Query: 252 -----YVREVIFKN----QISKED-FYDAIGNSPYAYDIT---AEHIQVTTDTMAKYGTG 298 ++ E K ++ +ED DAI + + I+ + +Q D + G+ Sbjct: 250 LIAGYFIAEPTLKTLKHGEMLREDVIRDAIKSQTFTPKISKKDQDRMQEIADILQSQGSL 309 Query: 299 K 299 K Sbjct: 310 K 310 >gi|300313870|ref|YP_003777962.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 323 Score = 66.2 bits (160), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 62/197 (31%), Positives = 96/197 (48%), Gaps = 14/197 (7%) Query: 1 MQQQKSKQKQWIALALLACAGFSGAS----HAADDVIRLGNLKFAHY----GAVSYIKEI 52 M + + K+ + L LA + + AAD V+R+G KF G+ + + Sbjct: 1 MDAHQPRHKRRLLLQGLAAGALASGTPLVRAAADKVLRIGYQKFNTLNILKGSGKLEQAL 60 Query: 53 APKCGIKVDEKVFAKGPDVMQGILAGELDVGTTASEAAISGRANGA-PIYVVA--GFAKG 109 P+ G V FA GP + + + A +D+G A ++ A+G +Y+ A + KG Sbjct: 61 QPQ-GWSVKWFEFAAGPQLTEALNANAIDLGHAADTPSVFANASGVNAVYLGAEQPYPKG 119 Query: 110 GARLVAGKDSGIKSVKDLKGKKVGVTRGGIHEVLLDAELGQNGLSAKDVTIVYLA-FADL 168 VA ++S I+SV DLKGKKV RG + LL L + GLS +D+ VY+ AD+ Sbjct: 120 IGIFVA-QESPIRSVADLKGKKVATGRGWNVQFLLLKALEEVGLSYRDIEPVYVTNAADV 178 Query: 169 NQALLGKNIDAMMQSEP 185 A N+DA +P Sbjct: 179 RAAFQSGNVDAATLWDP 195 >gi|300310977|ref|YP_003775069.1| alkanesulfonates ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 327 Score = 60.8 bits (146), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 56/183 (30%), Positives = 82/183 (44%), Gaps = 12/183 (6%) Query: 50 KEIAPKCGIKVDEKVFAKGPDVMQGILAGELDVGTTASEAAISGRANGAP-IYVVAGFAK 108 K + P G++V FA GP +++ + G +D G + +A GA +Y Sbjct: 67 KRLQP-LGVEVKWAEFAAGPQLLEALNVGSVDFGYVGEAPPVFAQAAGADFVYTAYEIPT 125 Query: 109 GGAR-LVAGKDSGIKSVKDLKGKKVGVTRGGIHEVLLDAELGQNGLSAKDVTIVYLAFAD 167 A L+ K+S IKSV +LKGKK+ +G L+ A L + GL D+ VYLA AD Sbjct: 126 PDAEGLLVQKNSPIKSVAELKGKKIAFNKGSDVHWLVVALLKKAGLQYSDIQPVYLAPAD 185 Query: 168 LNQALLGKNIDAMMQSEPQSSQAINKGFGVEVMKPYDTPIGEPYRTMVMTEKFYTEKRPL 227 A +DA +P + A K G + T +G V +F+ RP Sbjct: 186 ARAAFERGAVDAWAIWDPFQAAA-EKQVGARRLA---TGVG-----AVNHHQFFLSARPF 236 Query: 228 AEK 230 A K Sbjct: 237 AAK 239 >gi|300309986|ref|YP_003774078.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 328 Score = 58.9 bits (141), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 60/185 (32%), Positives = 93/185 (50%), Gaps = 15/185 (8%) Query: 13 ALALLACAGFSGASHAADD--VIRLGNLKFAHYGAVS-------YIKEIAPKCGIKVDEK 63 AL + A G S AA VIRL +A+Y S +++E G +V + Sbjct: 13 ALGVTALGGIPLLSSAAQPPKVIRL---DYAYYSPPSLVLRKFGWLEEDLKAQGTEV-KW 68 Query: 64 VFAKGPD-VMQGILAGELDVGTTASEAAISGRANGAPIYVVAGFAKG-GARLVAGKDSGI 121 V ++G + ++ + + +D G+TA AA+ RANG P+ V +++ L GK+S + Sbjct: 69 VLSQGSNRALEYLNSDSVDFGSTAGLAALLARANGNPVKAVYIYSRPEWTALAVGKESPV 128 Query: 122 KSVKDLKGKKVGVTRGGIHEVLLDAELGQNGLSAKDVTIVYLAFADLNQALLGKNIDAMM 181 KS+ +LKGKK+ T+G + L L +NGL DV IV+L AD AL + A Sbjct: 129 KSINELKGKKIAATKGTDPYLFLLRALHENGLKKSDVEIVHLQHADGRAALEQGRVAAWA 188 Query: 182 QSEPQ 186 +P Sbjct: 189 GLDPH 193 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.133 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 981,326 Number of extensions: 40344 Number of successful extensions: 163 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 160 Number of HSP's successfully gapped: 8 Length of query: 325 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 240 Effective length of database: 1,217,395 Effective search space: 292174800 Effective search space used: 292174800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)