BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0205.p (423 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309700|ref|YP_003773792.1| Mg2+/Co2+ transporter transmemb... 793 0.0 gi|300311018|ref|YP_003775110.1| CBS domains containing hemolysi... 105 9e-24 gi|300310418|ref|YP_003774510.1| hemolysin protein [Herbaspirill... 104 1e-23 gi|300310054|ref|YP_003774146.1| Mg2+ and Co2+ transporter prote... 104 1e-23 >gi|300309700|ref|YP_003773792.1| Mg2+/Co2+ transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 434 Score = 793 bits (2049), Expect = 0.0, Method: Compositional matrix adjust. Identities = 389/415 (93%), Positives = 406/415 (97%) Query: 1 VQALALLILILLSAFFSMTETALMAANRHRLRHLAKQGNRAAITILWLLDRTERLLSLIL 60 VQ LAL ILILLSAFFSMTETALMAANRHRLRHLAKQGNRAAITILWLLDRTERLLSL+L Sbjct: 8 VQVLALFILILLSAFFSMTETALMAANRHRLRHLAKQGNRAAITILWLLDRTERLLSLVL 67 Query: 61 IANTLINALATALVTALAIAAFGNHQRVITAATAFVAALLIIFAEIVPKVIGATHPERIA 120 IANT INALATALVTALAIAAFGNHQRVITAATA VAALLIIFAEIVPKVIGAT+PERI+ Sbjct: 68 IANTAINALATALVTALAIAAFGNHQRVITAATACVAALLIIFAEIVPKVIGATYPERIS 127 Query: 121 LFTSFILKPLMRVAKPLVWFVNIFVSAILKVLHIKTGARAAEQRISPEELRSIVLEGGSF 180 LFT FILKPLMRVAKPL+WFVN+FVSAIL++L IKTGARAAEQRISPEELRSIVLEGGSF Sbjct: 128 LFTGFILKPLMRVAKPLIWFVNLFVSAILRLLRIKTGARAAEQRISPEELRSIVLEGGSF 187 Query: 181 IPQKHKSILLNLFDLEKISVEDVMTPRAQVEALNLAAPVEEILHQLATCYHNKLPVFEGE 240 IPQKHKSILLNLFDLEKISVEDVMTPR+QVEALNLAAPV++ILHQLATCYHNKLPVFEGE Sbjct: 188 IPQKHKSILLNLFDLEKISVEDVMTPRSQVEALNLAAPVDDILHQLATCYHNKLPVFEGE 247 Query: 241 INHIVGILHVRKTVSLFRQEDDITHDDIRTLLSAPYYVPVETDVFTQLQYFQENHERLGI 300 INHIVGILHVRKTVSLFR+E++I+HDDIR LLSAPYYVPVETDVFTQLQYFQENHERLG+ Sbjct: 248 INHIVGILHVRKTVSLFRREEEISHDDIRELLSAPYYVPVETDVFTQLQYFQENHERLGV 307 Query: 301 VVDEYGEVQGLVTLEDIIEEMIGEFTTSSPGSSGEGFGWDKDGTCLLEGSTTLRDINKRL 360 VVDEYGEVQGLVTLEDIIEEMIGEFTTSSPGSSGEGFGWDKDG CLLEGSTTLRDINKRL Sbjct: 308 VVDEYGEVQGLVTLEDIIEEMIGEFTTSSPGSSGEGFGWDKDGACLLEGSTTLRDINKRL 367 Query: 361 GLKLPLDGPKTINGLLLETLQDIPEASVAVKIDGCVIEIVQVQNQAIRMVKLIAL 415 GL+LPLDGPKTINGLLLE LQDIPEASVAVK+DGCVIEIVQVQNQAIRMVKLIAL Sbjct: 368 GLQLPLDGPKTINGLLLEYLQDIPEASVAVKMDGCVIEIVQVQNQAIRMVKLIAL 422 >gi|300311018|ref|YP_003775110.1| CBS domains containing hemolysin protein [Herbaspirillum seropedicae SmR1] Length = 442 Score = 105 bits (261), Expect = 9e-24, Method: Compositional matrix adjust. Identities = 102/418 (24%), Positives = 195/418 (46%), Gaps = 37/418 (8%) Query: 13 SAFFSMTETALMAANRHRLRHLAKQGNRAAITILWLLDRTERLLSLILIANTLINALATA 72 SAF S E ++ A+ + RL ++K+G A +L L + +++ I +NA+A Sbjct: 17 SAFLSCAEISIAASRKIRLEMMSKEGVHNADRVLSLQAQPGNFFTVVQIG---LNAVAI- 72 Query: 73 LVTALAIAAFGNHQRVITA-------------ATAF--VAALLIIFAEIVPKVIGATHPE 117 L + A H R I + A +F V A I+FA+++PK + PE Sbjct: 73 LGGVVGEPALTPHMREIISSFYIGPWLENASFAMSFLVVTAFFILFADLMPKRLAMVAPE 132 Query: 118 RIALFTSFILKPLM---RVAKPLVWFVNIFVSAILKVLHIKTGARAAEQRISPEELRSIV 174 R A+ ++KP++ + KPLVWF N I + + + ++ I+P E+ ++V Sbjct: 133 RAAVA---LVKPMLLLETLFKPLVWFFNSLSKWIFTLFGL---PQVRQEVITPNEIHAMV 186 Query: 175 LEG---GSFIPQKHKSILLNLFDLEKISVEDVMTPRAQVEALNLAAPVEEILHQLATCYH 231 G G + Q+H ++ N+F+LE V MTPR + L I ++ H Sbjct: 187 DAGAQAGVLLTQEHH-LIENVFELESRYVPSAMTPRDSIVYFLLTDSEATIRQKIIDHPH 245 Query: 232 NKLPVFEGEINHIVGILHVRKTVSLFRQEDDITHDDIRTLLSAPYYVPVETDVFTQLQYF 291 ++ V +G I+ ++G + + + + + + D + L+ P +P +++ L+ Sbjct: 246 SRFLVCDGHIDAVLGYIDSKDVLKRLLKGEQFSLAD-KGLVHPPLSIPDSLNLWEVLERM 304 Query: 292 QENHERLGIVVDEYGEVQGLVTLEDIIEEMIGEFTTSSPGSSGEGFGWDKDGTCLLEGST 351 + E L IVV+EY V G+++ D+ ++G + + + + L++G T Sbjct: 305 KAMDEDLAIVVNEYALVVGIISTTDVTGVLMGNLLAIT--EEEQQIVRRDENSWLMDGLT 362 Query: 352 TLRDINKRLGL-KLPLD-GPKTINGLLLETLQDIPEASVAVKIDGCVIEIVQVQNQAI 407 L D+ L + + P G +T++G ++ L+ IP+ + V E+V ++ I Sbjct: 363 PLNDVMSELDIDEYPNPVGYETLSGFIMYMLRKIPKKTDFVLFAEFKFEVVDIEGNRI 420 >gi|300310418|ref|YP_003774510.1| hemolysin protein [Herbaspirillum seropedicae SmR1] Length = 440 Score = 104 bits (260), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 94/429 (21%), Positives = 192/429 (44%), Gaps = 31/429 (7%) Query: 7 LILILLSAFFSMTETALMAANRHRLRHLAK-QGNRAAITILWLLDRTERLLSLILIANTL 65 L+ L+ FF E +L+ + R+R +AK QG R I + + ++ + LS + TL Sbjct: 11 FFLVALNGFFVAAEFSLVKLRQTRIRAIAKTQGMRGRILAV-VHNQLDAYLSACQLGITL 69 Query: 66 IN---------ALATALVTALAIAAFGNHQRVITAATAF----VAALLIIFAEIVPKVIG 112 + A A L ++A N + + + F ++ L I+ E+ PK + Sbjct: 70 ASLGLGWIGEPAFARILEPLFSLAGVTNQELIHGVSFVFAFFVISFLHIVAGELAPKSMA 129 Query: 113 ATHPERIALFTSFILKPLMRVAKPLVWFVNIFVSAILKVLHIKTGARAAEQRISPEELRS 172 PE++ L+ + L PL+W +N + +L+V + G + S +EL+ Sbjct: 130 IRSPEKLGLWCAMPLYGFYWGMYPLIWVLNASSNWLLRVAGLGAG-HGHDAHYSSDELKL 188 Query: 173 IVLEG------GSFIPQKHKSILLNLFDLEKISVEDVMTPRAQVEALNLAAPVEEILHQL 226 I+ G G F + ++L + ++ V D+M P +++ AL +EE L + Sbjct: 189 ILRAGNKSGKNGKFT-RDEWNVLTQSLNFAELDVADIMRPASEIVALGDDKSLEENLDII 247 Query: 227 ATCYHNKLPVFEGEINHIVGILHVRKTVSLFRQEDDITHDDIRTLLSAPYYVPVETDVFT 286 +++ P ++ E ++G++H++ Q+D +++ L Y+ Sbjct: 248 YRNRYSRYPYYDAERQQVLGLVHLKDV--FLAQQDGRAIANLKDYLRPVQYISPALPALD 305 Query: 287 QLQYFQENHERLGIVVDEYGEVQGLVTLEDIIEEMIGEFTTSSPGSSGEGFGWDK--DGT 344 L+ F+ ++ + G +TL++++ ++GE ++GE W + DGT Sbjct: 306 LLRRFRTGSPHFAVIGKKGQPPAGFITLDNMLSLLVGEIRDEFRHNTGE---WTRQDDGT 362 Query: 345 CLLEGSTTLRDINKRLGLKLPLDGP-KTINGLLLETLQDIPEASVAVKIDGCVIEIVQVQ 403 L +GS + + LG+ + D ++ GL++E L D+P+ + + I ++ Sbjct: 363 LLGKGSLPIVTLENMLGIDIEYDDSIDSVGGLVMEKLGDLPKEGQKIDFPAFDVVIKRMS 422 Query: 404 NQAIRMVKL 412 I +VK+ Sbjct: 423 GPKIVLVKV 431 >gi|300310054|ref|YP_003774146.1| Mg2+ and Co2+ transporter protein [Herbaspirillum seropedicae SmR1] Length = 292 Score = 104 bits (259), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 59/235 (25%), Positives = 120/235 (51%), Gaps = 9/235 (3%) Query: 187 SILLNLFDLEKISVEDVMTPRAQVEALNLAAPVEEILHQLATCYHNKLPVFEGEINHIVG 246 S++ +F + ++V D+M PR+Q++ ++++ P+E + ++ H++ P +GE + +VG Sbjct: 55 SMIEGVFQVSDLAVRDIMVPRSQMDTIDISKPIETWMPEVLATSHSRFPAVDGERDKVVG 114 Query: 247 ILHVRKTVSLFRQEDDITHDDIRTLLSAPYYVPVETDVFTQLQYFQENHERLGIVVDEYG 306 IL + + + +E D+R +L ++P + L+ F+ N + +VVDEYG Sbjct: 115 ILLAKDLLRYYAEE----SFDVRDMLRPAIFIPESKRLNVLLRDFRANRNHMAVVVDEYG 170 Query: 307 EVQGLVTLEDIIEEMIGEFTTSSPGSSGEGFGWD-KDGT----CLLEGSTTLRDINKRLG 361 V GL+T+ED++E+++G+ E KDG ++ T + N+ +G Sbjct: 171 GVAGLITIEDVLEQIVGDIEDEYDFDEAEDNVLALKDGPHGARWRVKALTEIEQFNEEVG 230 Query: 362 LKLPLDGPKTINGLLLETLQDIPEASVAVKIDGCVIEIVQVQNQAIRMVKLIALP 416 + D TI GL+ + +P IDG E+++ + + ++ + LP Sbjct: 231 SRFSDDDADTIGGLVANHVGRVPRKGDEFDIDGLHFEVLRADARQVHVLLVSQLP 285 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.138 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,114,301 Number of extensions: 43423 Number of successful extensions: 134 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 124 Number of HSP's successfully gapped: 4 Length of query: 423 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 335 Effective length of database: 1,203,190 Effective search space: 403068650 Effective search space used: 403068650 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 132 (55.5 bits)