BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0184.p (843 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309679|ref|YP_003773771.1| UDP-N-acetylmuramate-L-alanine ... 885 0.0 gi|300309678|ref|YP_003773770.1| UDP-N-acetylglucosamine-N-acety... 488 e-139 gi|300309737|ref|YP_003773829.1| UDP-N-acetylmuramate:L-alanyl-g... 114 3e-26 >gi|300309679|ref|YP_003773771.1| UDP-N-acetylmuramate-L-alanine ligase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 885 bits (2287), Expect = 0.0, Method: Compositional matrix adjust. Identities = 464/480 (96%), Positives = 466/480 (97%), Gaps = 5/480 (1%) Query: 364 MKHRVKNIHFVGIGGSGMSGIAEVLLNLGYGVSGSDLGSNAATQRLAQLGASIRYGHAAE 423 MKHRVKNIHFVGIGGSGMSGIAEVLLNLGYGVSGSDLGSNAATQRLAQLGA IRYGHAA Sbjct: 1 MKHRVKNIHFVGIGGSGMSGIAEVLLNLGYGVSGSDLGSNAATQRLAQLGACIRYGHAAG 60 Query: 424 NIEGADAIVTSTAVKGDNPEVLAARKKHIPIVPRAVMLGELMRLKKGIAIAGTHGKTTTT 483 NIEGADAIVTSTAVKGDNPEVLAARKKHIPIVPRAVMLGELMRLKKGIAIAGTHGKTTTT Sbjct: 61 NIEGADAIVTSTAVKGDNPEVLAARKKHIPIVPRAVMLGELMRLKKGIAIAGTHGKTTTT 120 Query: 484 SLVASVLAQGGLDPTFVIGGPQGDCGLTSAGANAKLGTGEFIVAEADESDASFLNLTPVI 543 SLVASVLAQGGLDPTFVIGG LTSAGANAKLGTGEFIVAEADESDASFLNLTPVI Sbjct: 121 SLVASVLAQGGLDPTFVIGGR-----LTSAGANAKLGTGEFIVAEADESDASFLNLTPVI 175 Query: 544 EVITNIDADHMETYNHDFARLKQAFVEFTQRLPFYGVAVLCLDDPHVREIMPMVSKPILT 603 EVITNIDADHMETYNHDFARLKQAFVEFTQRLPFYGVAVLCLDDPHVREIMPMVSKPILT Sbjct: 176 EVITNIDADHMETYNHDFARLKQAFVEFTQRLPFYGVAVLCLDDPHVREIMPMVSKPILT 235 Query: 604 YGFHEDADVRAIDAKAVDGKMEFTVIQDGYAPMQVSLNQPGMHNVQNACAAIAIARELDV 663 YGFHEDADVRAIDAKAVDGKMEFTVIQDGYAPMQVSLNQPGMHNVQNACAAIAIARELDV Sbjct: 236 YGFHEDADVRAIDAKAVDGKMEFTVIQDGYAPMQVSLNQPGMHNVQNACAAIAIARELDV 295 Query: 664 DDAATQKALTEFNGVGRRFTRYGEVPITDAQGNPAGHFTLVDDYGHHPVETAATIAAARG 723 DDAATQKALTEFNGVGRRFTRYGEVP+ DA+G PAGHFTLVDDYGHHPVETAATIAAARG Sbjct: 296 DDAATQKALTEFNGVGRRFTRYGEVPLWDAEGKPAGHFTLVDDYGHHPVETAATIAAARG 355 Query: 724 AYPGRRLVLAFQPHRYTRTRDLFEDFVKVLSSPDVLVLGEVYSAGEAPIVAADGRSLAHA 783 AYPGRRLVLAFQPHRYTRTRDLFEDFVKVLSSPDVLVL EVYSAGEAPIVAADGRSLAHA Sbjct: 356 AYPGRRLVLAFQPHRYTRTRDLFEDFVKVLSSPDVLVLAEVYSAGEAPIVAADGRSLAHA 415 Query: 784 LRAAGKVEPVFVDDIGDMPETIRNVVRDGDVVMTMGAGSISAVPGRLVAQAGGKNEQGAA 843 LRAAGKVEPVFVDDIGDMPETIRNVVRDGDVVMTMGAGSISAVPGRL AQA KNEQGAA Sbjct: 416 LRAAGKVEPVFVDDIGDMPETIRNVVRDGDVVMTMGAGSISAVPGRLTAQADVKNEQGAA 475 >gi|300309678|ref|YP_003773770.1| UDP-N-acetylglucosamine-N-acetylmuramyl-pentapeptide pyrophosphoryl-undecaprenol N-acetylglucosamine transferase [Herbaspirillum seropedicae SmR1] Length = 360 Score = 488 bits (1256), Expect = e-139, Method: Compositional matrix adjust. Identities = 238/267 (89%), Positives = 250/267 (93%) Query: 1 MNAPRKLLIMAAGTGGHIFPGLAIADTMRARGWQVSWLGTTHGMERELVPRHGVEMDSIV 60 M+APRKLLIMAAGTGGHIFPGLAIADTMRARGW+VSWLGT+HGMERELVP HGVEMDSIV Sbjct: 1 MSAPRKLLIMAAGTGGHIFPGLAIADTMRARGWEVSWLGTSHGMERELVPCHGVEMDSIV 60 Query: 61 FAGLRGKGLMHTVKGVWRLAASFLHCHAILGRRRPDVVLGMGGYVTVPGGMMARLRGKPL 120 FAGLRGKGLMHTVKG WRL ASF C AILGRRRPDVVLGMGGYVTVPGG MARLRGKPL Sbjct: 61 FAGLRGKGLMHTVKGAWRLVASFFDCFAILGRRRPDVVLGMGGYVTVPGGAMARLRGKPL 120 Query: 121 VLVNADAALLLSNKTLTPMATKVLFGFPADFGKAAGKAEVTGNPVRKEIIALPQPAQRYA 180 VLVNADAALLLSNK LTP+A KVLFGFPADFGKAAGKAEVTGNPVR+EI ALP P QRYA Sbjct: 121 VLVNADAALLLSNKALTPLADKVLFGFPADFGKAAGKAEVTGNPVRQEITALPPPVQRYA 180 Query: 181 GRSGPLKILVVGGSLGAKVLNDNVPAALAKLPAELRPQVTHQSGKQHIAALRSAYEAAGV 240 GR GPLKILVVGGSLGAKVLN+ VPAALA+LP E RPQVTHQSGKQ+I ALR+AYEAAGV Sbjct: 181 GRRGPLKILVVGGSLGAKVLNETVPAALAQLPLEQRPQVTHQSGKQNIVALRAAYEAAGV 240 Query: 241 QAEVVDFIDDMPRRYAEADLVICRAGA 267 +AEV+DFI+DMPRRYAEADLVICRAGA Sbjct: 241 EAEVLDFIEDMPRRYAEADLVICRAGA 267 >gi|300309737|ref|YP_003773829.1| UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso- diaminopimelate ligase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 460 Score = 114 bits (285), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 111/384 (28%), Positives = 173/384 (45%), Gaps = 28/384 (7%) Query: 370 NIHFVGIGGSGMSGIAEVLLNLGYGVSGSDLGSN--AATQRLAQLGASIRYGHAAENIE- 426 +IH +GI G+ M G+A + G+ V+G D +TQ AQ G ++ G A +E Sbjct: 2 HIHILGICGTFMGGLAVLAKEAGHTVTGCDANVYPPMSTQLEAQ-GITLTQGFDAAQVEL 60 Query: 427 GADAIVTSTAVKGDNPEVLAARKKHIPIVPRAVMLGE-LMRLKKGIAIAGTHGKTTTTSL 485 D V V NP + + +P V +GE +++ K +A+AGTHGKTTT+++ Sbjct: 61 EPDLFVIGNVVSRGNPLMEEILNRGLPYVSGPQWMGENILQDKWVLAVAGTHGKTTTSAM 120 Query: 486 VASVLAQGGLDPTFVIGGPQGDCGLTS-AGANAKLGTGEFIVAEADESDASFLNLTPVI- 543 +A VL G P F+IGG + G+++ G++ + G F V EADE D +F + Sbjct: 121 LAWVLEHAGYQPGFLIGGVPMNFGVSARLGSDGQGGPAPFFVIEADEYDTAFFDKRSKFV 180 Query: 544 ------EVITNIDADHMETYNHDFARLKQAFVEFTQRLPFYGVAVLCLDDPHVREIMPM- 596 V+ N++ DH + + D A ++ F + +P G ++ +P ++ ++ Sbjct: 181 HYRTRTAVLNNLEYDHADIFP-DLAAIETQFHHLVRTVPGIGRLIVNGREPALQRVLARG 239 Query: 597 VSKPILTYGFHEDADVRAIDAKAVDGKMEFTVIQDGYAPMQVSLNQPGMHNVQNACAAIA 656 +G + ++ DG+ F V DG V G HN NA AAIA Sbjct: 240 CWSEREDFGVAQGQSGWSLLTHE-DGR--FDVFFDGQTQGTVEWQLTGEHNRMNALAAIA 296 Query: 657 IARELDVDDAATQKALTEFNGVGRRFTRYGEVPITDAQGNPAGHFTLVDDYGHHPVETAA 716 AR + V A AL F V RR G A + DD+ HHP A Sbjct: 297 AARHVGVPVAQAIAALGAFQSVKRRMELRGS----------ANGVAVYDDFAHHPTAIAT 346 Query: 717 TIAAARGAYPGRRLVLAFQPHRYT 740 T+A R R++ +P T Sbjct: 347 TVAGLRKKVGAARILAVLEPRSNT 370 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,853,600 Number of extensions: 129864 Number of successful extensions: 481 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 474 Number of HSP's successfully gapped: 3 Length of query: 843 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 750 Effective length of database: 1,179,515 Effective search space: 884636250 Effective search space used: 884636250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 135 (56.6 bits)