BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0178.p (592 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309672|ref|YP_003773764.1| cell division FtsI protein/peni... 1197 0.0 gi|300313947|ref|YP_003778039.1| cell division protein FtsI [Her... 127 2e-30 gi|300313465|ref|YP_003777557.1| penicillin-binding 1 (peptidogl... 57 4e-09 >gi|300309672|ref|YP_003773764.1| cell division FtsI protein/penicillin-binding protein 2 [Herbaspirillum seropedicae SmR1] Length = 592 Score = 1197 bits (3097), Expect = 0.0, Method: Compositional matrix adjust. Identities = 588/592 (99%), Positives = 590/592 (99%) Query: 1 MTKMPPRTNLSGGRVAASKGVAFSSNPILQVKLPAWRSRFVLFVLFAAFVALVLRALWLQ 60 MTKMPPRTNLSGGRVAASKGVAFSSNPILQVKLPAWRSRFVLFVLF AFVALVLRALWLQ Sbjct: 1 MTKMPPRTNLSGGRVAASKGVAFSSNPILQVKLPAWRSRFVLFVLFVAFVALVLRALWLQ 60 Query: 61 GVSTDFLQKQGASRYARTLELPATRGKITDRNGQVLASSVPVKAIWAIPEDVQAAPKDKL 120 GVSTDFLQKQGASRYARTLELPATRGKITDRNGQVLASSVPVKAIWAIPEDVQAAPKDKL Sbjct: 61 GVSTDFLQKQGASRYARTLELPATRGKITDRNGQVLASSVPVKAIWAIPEDVQAAPKDKL 120 Query: 121 VQLAKLLEMSDVELRKKLDSDRSFVYLKRQVEQDTADKIVKLGIPGIETRKEYKRFYPEG 180 VQLAKLLEMSD +LRKKLDSDRSFVYLKRQVEQDTADKIVKLGIPGIETRKEYKRFYPEG Sbjct: 121 VQLAKLLEMSDADLRKKLDSDRSFVYLKRQVEQDTADKIVKLGIPGIETRKEYKRFYPEG 180 Query: 181 EVMAHVVGFTNIEDAGQDGMELAAQKTLAGVTGSRRVIKDRLGRVVEDIESIREPHDGRD 240 EVMAHVVGFTNIEDAGQDGMELAAQKTLAGVTGSRRVIKDRLGRVVEDIESIREPHDGRD Sbjct: 181 EVMAHVVGFTNIEDAGQDGMELAAQKTLAGVTGSRRVIKDRLGRVVEDIESIREPHDGRD 240 Query: 241 LTLSIDSKIQYIAFTQLKEAVEKFKAKAGGIIVVDAKTGEVLALANLPTYNPNDRSVLTG 300 LTLSIDSKIQYIAFTQLKEAVEKFKAKAGGIIVVDAKTGEVLALANLPTYNPNDRSVLTG Sbjct: 241 LTLSIDSKIQYIAFTQLKEAVEKFKAKAGGIIVVDAKTGEVLALANLPTYNPNDRSVLTG 300 Query: 301 AQLRNRVVTDTFEPGSTLKPFTVALALDTHRVTPSTVFQTAPGKMTIGTATIGDSHAHGP 360 AQLRNRVVTDTFEPGST+KPFTVALALDTHRVTPSTVFQTAPGKMTIGTATIGDSHAHGP Sbjct: 301 AQLRNRVVTDTFEPGSTMKPFTVALALDTHRVTPSTVFQTAPGKMTIGTATIGDSHAHGP 360 Query: 361 LTVAQIIEKSSNIGTAKIALGMPPEEMWEMFTTVGFGQQPKFGFPGAVAGRVRPYKSWRP 420 LTVAQIIEKSSNIGTAKIALGMPPEEMWEMFTTVGFGQQPKFGFPGAVAGRVRPYKSWRP Sbjct: 361 LTVAQIIEKSSNIGTAKIALGMPPEEMWEMFTTVGFGQQPKFGFPGAVAGRVRPYKSWRP 420 Query: 421 IEQATMSYGHGISVSLIQLAHAYLIFARNGDIIPLSFTKVTDSPIGQRVISADTALQMRR 480 IEQATMSYGHGISVSLIQLAHAYLIFARNGDIIPLSFTKVTDSPIGQRVISADTALQMRR Sbjct: 421 IEQATMSYGHGISVSLIQLAHAYLIFARNGDIIPLSFTKVTDSPIGQRVISADTALQMRR 480 Query: 481 MLETVVAPGGTAPQAQVPGYRVGGKTGTAYKIEGGKYVRKYVASFSGIAPMSDPRLIISV 540 MLETVVAPGGTAPQAQVPGYRVGGKTGTAYKIEGGKYVRKYVASFSGIAPMSDPRLIISV Sbjct: 481 MLETVVAPGGTAPQAQVPGYRVGGKTGTAYKIEGGKYVRKYVASFSGIAPMSDPRLIISV 540 Query: 541 MIDEPQGQHYGGPVAGPVFANVAANALRALNVPPDSSVTNIIIPKEPLEESM 592 MIDEPQGQHYGGPVAGPVFANVAANALRALNVPPDSSVTNIIIPKEPLEESM Sbjct: 541 MIDEPQGQHYGGPVAGPVFANVAANALRALNVPPDSSVTNIIIPKEPLEESM 592 >gi|300313947|ref|YP_003778039.1| cell division protein FtsI [Herbaspirillum seropedicae SmR1] Length = 656 Score = 127 bits (319), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 151/600 (25%), Positives = 236/600 (39%), Gaps = 113/600 (18%) Query: 48 AFVALVLRALWLQGVSTDFLQKQGASRYARTLELPATRGKITDRNGQVLASSVPVKAIWA 107 F L+ R LWLQ V D Q + + RG I DRNG VLA + + Sbjct: 28 CFSLLLARFLWLQVVRHDAYAAQAEENRISVVPIVPNRGLILDRNGVVLARNYSAYTLEI 87 Query: 108 IPEDVQAAPKDKLVQLAKLLEMS------DVELRKKLDSDRSFVYLKRQVEQDTADKIV- 160 P ++ D + +L L+E+ L+++ + S R +++ A Sbjct: 88 TPSKIRTNIDDLIDELGTLVEIQPRDRRRFRRLQEESKNFESVPIRTRLTDEEVAKFTAQ 147 Query: 161 KLGIPGIETRKEYKRFYPEGEVMAHVVGF---------TNIEDA------------GQDG 199 + PG+E + R YP G+ AHV+G+ I D+ G++G Sbjct: 148 RYRFPGVEVQARLFRQYPYGKTAAHVIGYIGRISQRDAERIADSDDEANYNGTDYIGKEG 207 Query: 200 MELAAQKTLAGVTGSRRVIKDRLGRVVEDIESIREPHD-GRDLTLSIDSKIQYIAFTQLK 258 +E + + L G TG V GR V + R P G++L LSID ++Q K Sbjct: 208 LEKSYESQLHGTTGYEEVEISASGRAVRSLS--RTPATPGKNLILSIDIELQ-------K 258 Query: 259 EAVEKFKAKAGGIIVVDAKTGEVLALANLPTYNPN------------------DRSVLTG 300 E F + G ++ ++ TG++LA + P+Y+PN DR +L Sbjct: 259 VVEEAFGDQKGALVAINPATGDILAYVSQPSYDPNLFVEGIDQQSWDELNKSPDRPLL-- 316 Query: 301 AQLRNRVVTDTFEPGSTLKPFTVALALDTHRVTPSTVFQTAPGKMTIGTATIGDSH--AH 358 NR ++ + PGST KPF AL+ + P + PG +G D H Sbjct: 317 ----NRPLSGLYSPGSTYKPFMALAALELGKRRPQDAIRD-PGYFILGNHRFRDDKEGGH 371 Query: 359 GPLTVAQIIEKSSNIGTAKIALGMPPEEMWEMFTTVGFGQ-------QPKFG-FPGA--- 407 G + + + + S + +A M + + GFGQ K G P Sbjct: 372 GVVDMYRSVVVSCDTYYYMLANDMGVNAIHDFMKPFGFGQITGIDLEHEKRGILPSTEWK 431 Query: 408 -VAGRVRPYKSWRPIEQATMSYGHGI-SVSLIQLAHAYLIFARNGDIIPLSFTKVTDS-- 463 A R K W + ++ G G S + +QLA A I A NG ++ K+ + Sbjct: 432 RTAFRKPEQKRWIAGDTVSLGIGQGYNSFTPLQLAQATAILANNGVVMKPHLVKIVEDGV 491 Query: 464 ---------------PIGQRVISADTALQMRRMLETVVAPGGTAPQAQVPGYRVGGKTGT 508 P+ Q I ++R + VV G A + Y GGKTGT Sbjct: 492 THERTETVPKESYRIPMKQENIDF-----IKRAMVGVVKEGTGAAAFRGAPYESGGKTGT 546 Query: 509 A--------YKIEGGKYVRKYVAS--FSGIAPMSDPRLIISVMIDEPQGQHYGGPVAGPV 558 A K + K R Y + + G AP PR+ I+ ++ + +G VA P+ Sbjct: 547 AQVVNIAKNQKYDSKKLSRIYHDNGLYIGFAPADAPRIAIAAVV---ENGGWGAGVAAPL 603 >gi|300313465|ref|YP_003777557.1| penicillin-binding 1 (peptidoglycan synthetase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 771 Score = 57.0 bits (136), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 80/321 (24%), Positives = 129/321 (40%), Gaps = 63/321 (19%) Query: 271 IIVVDAKTGEVLALANLPTYNPNDRSVLTGAQLRNRVVTDTFEPGSTLKPFTVALALDTH 330 + V+++ G + +L +N N N V +PGS+ KPF + +L+ Sbjct: 433 FVSVNSQDGAIRSLVGGFDFNRNKF---------NHVTQAWRQPGSSFKPFIYSSSLEKG 483 Query: 331 RVTPSTVFQTAPGKMTIGTATIGDSHAHG-----PLTVAQIIEKSSNIGTAKIALGMPPE 385 ++P+T+ AP T + +G P+++ + ++KS N+ + +I + + Sbjct: 484 -LSPATIINDAPLTFTQTGGQVWQPKNYGGKFEGPISMRRALQKSINLVSIRILERVGVK 542 Query: 386 EMWEMFTTVGFGQQPKFGFPGAVAGRVRPYKSWRPIEQATMSYGHGISVSLIQLAHAYLI 445 E T GF A + PY T++ G G +V+ +Q+ AY + Sbjct: 543 YAQEYITRFGFD-----------ADKNPPY--------LTLALGAG-NVTPLQMVGAYSV 582 Query: 446 FARNG-DIIPLSFTKVTDS--------------PIGQRVISADTALQMRRMLETVVAPGG 490 FA G I P TKVTDS RVI A A M ML VV G Sbjct: 583 FANGGYKINPYLITKVTDSNGEVLSQAKPATADDESNRVIDARNAFIMDSMLRDVVRHGT 642 Query: 491 TAPQAQVPGYRVGGKTGTAYKIEGGKYVRKYVASFSGIAPMSDPRLIISVMIDEPQG--- 547 + + GKTGT A F+G P I + D+P+ Sbjct: 643 AVKALSLKRTDLAGKTGTTND--------SMDAWFAGYQP--GLTAIAWIGYDQPKSLGD 692 Query: 548 QHYGGPVAGPVFANVAANALR 568 + GG +A PV+ + + AL+ Sbjct: 693 RETGGGLALPVWISYMSKALK 713 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,759,261 Number of extensions: 71130 Number of successful extensions: 260 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 255 Number of HSP's successfully gapped: 3 Length of query: 592 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 501 Effective length of database: 1,188,985 Effective search space: 595681485 Effective search space used: 595681485 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)