BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0174.p (152 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309670|ref|YP_003773762.1| S-adenosyl-methionine-dependent... 230 3e-62 >gi|300309670|ref|YP_003773762.1| S-adenosyl-methionine-dependent methyltransferase MraW protein [Herbaspirillum seropedicae SmR1] Length = 324 Score = 230 bits (587), Expect = 3e-62, Method: Compositional matrix adjust. Identities = 114/126 (90%), Positives = 118/126 (93%) Query: 1 MNLQPVPELQHRTVLLEEAVDALAIAGERANGIYLDGTFGRGGHSRAILSRLGPQGRLIA 60 MNLQPVPELQHRTVLLEEAVDALAI GERANG+YLDGTFGRGGHSRAILSRLGPQGRLIA Sbjct: 1 MNLQPVPELQHRTVLLEEAVDALAIEGERANGVYLDGTFGRGGHSRAILSRLGPQGRLIA 60 Query: 61 FDKDPLAIATAQKIDDARFEIVHDSFATMDEALAARGIAQVDGVLMDLGISSPQVDDAAR 120 FDKDPLAIATAQKI D RFEIVHDSFATMD+ALA RGIA+VDGVLMDLGISSPQVDDAAR Sbjct: 61 FDKDPLAIATAQKIADPRFEIVHDSFATMDQALAERGIARVDGVLMDLGISSPQVDDAAR 120 Query: 121 GIXLQF 126 G + Sbjct: 121 GFSFRL 126 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.138 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 545,727 Number of extensions: 23195 Number of successful extensions: 73 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 73 Number of HSP's successfully gapped: 1 Length of query: 152 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 75 Effective length of database: 1,255,275 Effective search space: 94145625 Effective search space used: 94145625 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)