BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0170.p (386 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309667|ref|YP_003773759.1| enoyl-coenzyme A hydratase [Her... 698 0.0 gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspiri... 72 9e-14 gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [... 68 1e-12 gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspiri... 65 9e-12 gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspiri... 61 2e-10 >gi|300309667|ref|YP_003773759.1| enoyl-coenzyme A hydratase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 698 bits (1802), Expect = 0.0, Method: Compositional matrix adjust. Identities = 336/385 (87%), Positives = 355/385 (92%) Query: 1 MNQTAAPVVFEELTAANGRRIGIATLASEKTLHAISLEMVRLLTPQLQQWQADPGVAMVI 60 MNQ AAPVVFEELTAANGRRIGIATLASEKTLHAISLEMV+LLTPQLQ WQADP VAMV+ Sbjct: 1 MNQAAAPVVFEELTAANGRRIGIATLASEKTLHAISLEMVQLLTPQLQHWQADPEVAMVV 60 Query: 61 LQAQGEKAFCAGGDLQQLYRSMREHHASPAREDIRANTYAAEFFEHEYRLDYLVHSFAKP 120 LQAQGEKAFCAGGDLQQLYRSMREHHASPAREDIRAN YAAEFFE EYRLDYL+HSF KP Sbjct: 61 LQAQGEKAFCAGGDLQQLYRSMREHHASPAREDIRANRYAAEFFEQEYRLDYLIHSFTKP 120 Query: 121 ILCWGHGIAMGGGIGLMAGCSHRVVTEKSRLAMPEITIGLYPDVGGSWFLARMPGKTGIF 180 ILCWGHGI MGGGIGLMAGCSHRVVTEKSRLAMPEITIGLYPDVGGSWFLARMPGK GIF Sbjct: 121 ILCWGHGIVMGGGIGLMAGCSHRVVTEKSRLAMPEITIGLYPDVGGSWFLARMPGKIGIF 180 Query: 181 LALTGANLNANDALFAGLADYRVAHAAKASVIDALLMQPWGQDNDAELLDRVLASAQASS 240 LALTGANLNA DALFAGLADY +AHA+KA+V DALL QPWGQ DAELLDRVLA AQA+S Sbjct: 181 LALTGANLNAQDALFAGLADYGMAHASKAAVFDALLSQPWGQGRDAELLDRVLAGAQAAS 240 Query: 241 SQEGAAVFAAANLRTHFDLIESLCRRPTLPEIVDGILTLETEDAWLVKAQATLRAGSPGS 300 +EG A F +NLR+HFD IE+LCRRPTLPEIVD IL ++T+DAWL+KAQ+TLRAG+PGS Sbjct: 241 RREGVAAFVPSNLRSHFDQIEALCRRPTLPEIVDAILAVQTDDAWLLKAQSTLRAGAPGS 300 Query: 301 AWLGYALQKRVRPLSLAQVFRLEFVVSLHCAARPDFVEGIRALIIEKDQKPQWNPASLAA 360 AWLGYALQK+VRPLSLA+VFRLEFVVSLHCAARPDFVEGIRALIIEKDQKPQW PASL Sbjct: 301 AWLGYALQKKVRPLSLAEVFRLEFVVSLHCAARPDFVEGIRALIIEKDQKPQWKPASLKQ 360 Query: 361 ATPQWVEGFFADPWPQADHPLADLG 385 ATP WVEGFFADPW Q HPLADLG Sbjct: 361 ATPAWVEGFFADPWTQGGHPLADLG 385 >gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 263 Score = 71.6 bits (174), Expect = 9e-14, Method: Compositional matrix adjust. Identities = 61/199 (30%), Positives = 89/199 (44%), Gaps = 22/199 (11%) Query: 22 GIATLASEKTLH--AISLEMVRLLTPQLQQWQADPGVAMVILQAQGEKAFCAGGDLQQLY 79 GI TL + H A+S EM+ L QL ++D V VIL A G KAFCAG DL+Q+ Sbjct: 16 GITTLTLNRPQHFNALSEEMLTALQAQLDDIKSDSTVRCVILAANG-KAFCAGHDLRQM- 73 Query: 80 RSMREHHASPAREDIRANTYAAEFFEHEYRLDYLVHSFAK---PILCWGHGIAMGGGIGL 136 A+P + TY F +R +L+ S + P++ G+A G L Sbjct: 74 ------QATPQQ------TYYEALF---HRCSHLMQSLRELPVPVIAKVQGLATAAGCQL 118 Query: 137 MAGCSHRVVTEKSRLAMPEITIGLYPDVGGSWFLARMPGKTGIFLALTGANLNANDALFA 196 +A C + E +R A+ I +GL+ +P K + +TG ++A A Sbjct: 119 VATCDLAIAAESARFAVSGINVGLFCSTPAVALTRNLPIKRAFEMLVTGKFIDAATAAAW 178 Query: 197 GLADYRVAHAAKASVIDAL 215 GL + V S AL Sbjct: 179 GLINQAVPEQELDSATQAL 197 >gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [Herbaspirillum seropedicae SmR1] Length = 262 Score = 68.2 bits (165), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 57/193 (29%), Positives = 91/193 (47%), Gaps = 19/193 (9%) Query: 8 VVFEELTAA-NGRRIGIATLASEKTLHAISLEMVRLLTPQLQQWQADPGVAMVILQAQGE 66 V +E+L AA +G + TL +A+ + +R + L + + D V +V++ E Sbjct: 5 VAYEDLLAARHGEHVLHLTLNRPALRNALRNQSLREIVAALARAEEDDSVRVVVISGN-E 63 Query: 67 KAFCAGGDLQQLYRSMREHHASPAREDIRANTYAAEFFEHEYRLDYLVHSFAKPILCWGH 126 KAF AG DL + M A + D+RA + A + F KPIL + Sbjct: 64 KAFAAGADLNE----MIHKDAIATQLDVRAQYWRA------------IARFPKPILAAVN 107 Query: 127 GIAMGGGIGLMAGCSHRVVTEKSRLAMPEITIGLYPDVGGSWFLARMPGK-TGIFLALTG 185 G A+G G L+ + +++ PEI +G P GG+ L R GK + + L+G Sbjct: 108 GYALGAGCELLMHADIAIAARGAKIGQPEINVGTLPGAGGTQRLIRTVGKPLAMKMVLSG 167 Query: 186 ANLNANDALFAGL 198 ++A+ AL AGL Sbjct: 168 EFISADQALQAGL 180 >gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 258 Score = 65.1 bits (157), Expect = 9e-12, Method: Compositional matrix adjust. Identities = 53/198 (26%), Positives = 86/198 (43%), Gaps = 21/198 (10%) Query: 10 FEELTAANGRRIGIATLASEKTLHAISLEMVRLLTPQLQQWQADPGVAMVILQAQGEKAF 69 +E + ++G+ L K L+A+S ++ L L + A + +I+ EKAF Sbjct: 3 YENIQVETHEKVGLIRLHRPKALNALSDGLMTELGEALLAFDAQEEIGCIIITGS-EKAF 61 Query: 70 CAGGDLQQL--YRSMREHHASPAREDIRANTYAAEFFEHEYRLDYLVHSFAKPILCWGHG 127 AG D+ + Y M + + EF + + KP++ G Sbjct: 62 AAGADISAMAGYDYM--------------DVFKGEFITRNWET---LRRIRKPVIAAVAG 104 Query: 128 IAMGGGIGLMAGCSHRVVTEKSRLAMPEITIGLYPDVGGSWFLARMPGK-TGIFLALTGA 186 A+GGG L C + + ++ PEI +G+ P GG+ L R K + LALTG Sbjct: 105 YALGGGCELAMMCDFIIAADNAKFGQPEIKLGIVPGAGGTQRLPRAVSKAKAMDLALTGR 164 Query: 187 NLNANDALFAGLADYRVA 204 ++A +A AGL VA Sbjct: 165 MMDAEEAERAGLVSRIVA 182 >gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 60.8 bits (146), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 60/212 (28%), Positives = 94/212 (44%), Gaps = 35/212 (16%) Query: 43 LTPQLQQWQADPGVAMVILQAQGEKAFCAGGDLQQLYRSMREHHASPAREDIRANTYAAE 102 LT +++ AD + +++ +GEK F AG DL+ + + ARE R E Sbjct: 36 LTRLVKELNADRDIYTLVVTGEGEKFFSAGADLKLFA----DGDKAMAREMAR---RFGE 88 Query: 103 FFEHEYRLDYLVHSFAKPILCWGHGIAMGGGIGLMAGCSHRVVTEKSRLAMPEITIGLYP 162 FE + +F + +G AMGGG+ C R+ E++++A+PE +GL P Sbjct: 89 AFET-------LSAFRGVSIAAINGYAMGGGLECALACDIRIAEEQAQMALPEAAVGLLP 141 Query: 163 DVGGSWFLARMPGKT-GIFLALTGANLNANDALFAGLADYRVAHAAKASVIDALLMQPWG 221 GG+ L + G+ + L G +NA AL GL + V P G Sbjct: 142 CAGGTQNLPWLVGEGWAKRMILCGERVNAETALRIGLVEEVV---------------PRG 186 Query: 222 QDNDAELLDRVLASAQASSSQEGAAVFAAANL 253 Q +R LA AQ + Q ++V A+ L Sbjct: 187 QAR-----ERALALAQQAEKQSPSSVAASKQL 213 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.134 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,204,613 Number of extensions: 46822 Number of successful extensions: 158 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 154 Number of HSP's successfully gapped: 6 Length of query: 386 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 299 Effective length of database: 1,207,925 Effective search space: 361169575 Effective search space used: 361169575 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)